SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene9698

Length: 1357 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene9698

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Folylpolyglutamate synthase, putative n=1 Tax=Ricinus communis RepID=B9SE17_RICCO	-	-	0.0	65%
FL-Next	Isoform 2 of Folylpolyglutamate synthase OS=Arabidopsis thaliana GN=FPGS1	-	-	0.0	64%
Sma3	Folylpolyglutamate synthase	-	-	1.731e-22	-

Source	ECs	Term	Type	e value	Identity
Sma3	Tetrahydrofolate synthase.	EC:6.3.2.17	-	1.731e-22	-

Source	KEGGs	Term	e value	Identity
Sma3	Folate biosynthesis	00790	1.731e-22	%
Sma3	Biosynthesis of phenylpropanoids	01061	1.731e-22	%
Sma3	Metabolic pathways	01100	1.731e-22	%

Source	Gene names
Sma3	AT5G05980; At3g55630; At5g05980; F1I16_40; FPGS1; FPGS2; FPGS3; FPGS4; GSVIVT00018404001; GSVIVT00025328001; LOC_Os03g02030; LOC_Os10g35940; MICPUCDRAFT_25571; MICPUN_88042; OSJNBb0073N24.10; OSTLU_16744; Os03g0111100; Os10g0503200; OsI_09694; OsI_34225;

Source	GOs	Term	Type	Identity
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	tetrahydrofolylpolyglutamate synthase activity	GO:0004326	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	folic acid-containing compound biosynthetic process	GO:0009396	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Folylpolyglutamate synthetase	IPR001645	-	-
Sma3	Mur ligase, C-terminal	IPR004101	-	-
Sma3	Exostosin-like	IPR004263	-	-
Sma3	Mur ligase, central	IPR013221	-	-
Sma3	Folylpolyglutamate synthetase, conserved site	IPR018109	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G05980.1	ATDFB, DFB, FPGS1 DHFS-FPGS homolog B chr5:1799738-1804441 REVERSE LENGTH=571	64%
RefSeq	Arabidopsis thaliana	NP_001031840.1	DHFS-FPGS homolog B [Arabidopsis thaliana]	64%
RefSeq	Populus trichocarpa	XP_002311192.1	folylpolyglutamate synthase [Populus trichocarpa]	64%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: sp_plants

Fln subject: F4K2A1-2

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 137 aas, your sequence is shorter than subject: 330 - 513

Fln protein: MDKSGSPSHSVKYTDEEPNTVIETSNTSSSSSSYGAALDALSSLITPRIRANETEINFPIMFDYLKILDLEEPLSQLKVIHVAGTKGKGSTCTFVESILRKCGFRTGLYTSPHLINIFERYRLDGVEISEEKFLKYFWWCWNRLKENHLEYVHFFRFLTLLAFKIFTEEKVDVAILEVGLGGKFDATNVVKSPVVCGISSLGYDHMDILGNSLTEIAREKAGIMKPQVPAITVAQPEEAMEVLKDTASQLSIPLHVALPLETRMLGSMKLGLAGDHQYINASLAVALCHSWLRRTGHSEDFNLTESVCSCVLFGNIDCYFYEFMVYCKV*

Protein Length: 331

Fln nts: ATGGGGGATTCTGTCTCCTGAGCGAAACATTACTCACATATTGGCTGCTCCTGCTTCTTTGTGGCTTACATCAGACGAAAGTTATACTATATTGACTGTACTTGCCAAGGTAGCTGCTGTTCTTCGGATTCTCTCTTCTTGCCATTCACCTCTGCGTTGAATCGGGACC_-_ATGGACAAGTCAGGGAGTCCCTCGCATTCTGTCAAGTACACAGACGAGGAGCCGAACACTGTAATTGAAACGTCAAATACAAGTTCTTCATCCTCATCATATGGAGCAGCTTTGGATGCACTTTCTTCTCTCATCACTCCACGTATACGAGCAAATGAGACTGAAATCAATTTCCCTATTATGTTTGATTATTTGAAGATACTAGACTTGGAGGAACCTCTGTCCCAACTGAAAGTTATCCATGTTGCTGGAACGAAAGGGAAGGGTTCTACATGCACGTTTGTAGAAAGCATATTGCGGAAATGTGGCTTTCGAACTGGCCTTTACACATCCCCCCATCTGATTAATATTTTTGAGAGATATCGCCTTGATGGTGTGGAAATATCAGAAGAAAAGTTTCTGAAATACTTTTGGTGGTGTTGGAACCGTTTGAAGGAAAATCATTTAGAGTATGTACATTTTTTCCGATTCCTCACACTCTTGGCCTTCAAGATATTTACAGAAGAGAAGGTTGATGTTGCAATTTTGGAAGTAGGGTTGGGAGGGAAGTTTGATGCAACAAATGTGGTTAAATCACCAGTTGTCTGTGGAATATCTTCATTAGGATATGATCATATGGATATTCTAGGGAATTCTCTCACAGAAATTGCACGAGAGAAAGCTGGAATTATGAAGCCTCAAGTTCCAGCAATCACAGTAGCTCAACCTGAAGAAGCAATGGAAGTTCTGAAAGATACGGCATCCCAGTTATCGATTCCTCTTCATGTAGCATTGCCATTGGAGACTAGAATGCTTGGGAGTATGAAGCTAGGGTTGGCTGGTGATCATCAATATATTAATGCAAGCCTAGCAGTTGCCCTCTGTCATTCTTGGCTCAGGAGAACTGGACATTCAGAAGATTTTAATCTGACAGAGAGTGTTTGTTCATGTGTCCTTTTTGGAAATATTGATTGTTATTTTTATGAATTTATGGTTTATTGCAAGGTTTGAAATGAAAGGGCTCTAACTGATATGAGGCATGATGTTCATGCTCCAGGCAACTGAAGAAGGTTACTTGCCAGAGGCTTTCAAAGAAGGGCTTTCAATGGCCAATATTCTAGGTCGTGCACAGGTTATTCCTGATTCATACACGATGCACAATGAACACTGCAATATGAAAGAAGATGGAAATGCTGATTCTGGTCGGTT

Fln Alignment:

Contig9698___ITPRIRANETEIN--FPIMFDYLKILDLEEPLSQLKVIHVAGTKGKGSTCTFVESILRKCGFRTGLYTSPHLINIFERYRLDGVEISEEKFLKYFWWCWNRLKENHLEYVH---FFRFLTLLAFKIFTEEKVDVAILEVGLGGKFDATNVVKSPVVCGISSLGYDHMDILGNSLTEIAREKAGIMKPQVPAITVAQPEEAMEVLKDTASQLSIPLHVALPLETRMLGSMKLGLAGDHQYINASLAVALCHSWLRRTGHSE
F4K2A1-2____________ITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLE

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
280		80		20	0.995493
281	20			80	0.995493
282			20	80	0.995493
283			20	80	0.995493
696	20			80	0.995493
697	20	20		60	0.99255
700			80	20	0.995493
804	16			83	0.994512
806		83	16		0.994512
878		83	16		0.994512
983	14		85		0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain