SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene9601

Length: 1653 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene9601

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cytochrome P450 monooxygenase isoform I n=1 Tax=Sesamum indicum RepID=B3EXG1_SESIN	-	-	57%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	84%
Sma3	Flavonoid 3'-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Flavonoid 3'-monooxygenase.	EC:1.14.13.21	-	7.074e-07	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Flavonoid biosynthesis	00941		7.074e-07	%
Sma3	Flavone and flavonol biosynthesis	00944		7.074e-07	%
Sma3	Biosynthesis of phenylpropanoids	01061		7.074e-07	%
Sma3	Metabolic pathways	01100		7.074e-07	%
Sma3	Biosynthesis of secondary metabolites	01110		7.074e-07	%
Sma3	N-methylcoclaurine 3'-monooxygenase.	EC:1.14.13.71	-	6.888e-13	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Isoquinoline alkaloid biosynthesis	00950		6.888e-13	%
Sma3	Metabolic pathways	01100		6.888e-13	%
Sma3	Biosynthesis of secondary metabolites	01110		6.888e-13	%
Sma3	Flavonoid 3',5'-hydroxylase.	EC:1.14.13.88	-	3.031e-25	-

Source	KEGGs	Term	e value	Identity
Sma3	Flavonoid biosynthesis	00941	3.031e-25	%
Sma3	Flavone and flavonol biosynthesis	00944	3.031e-25	%
Sma3	Biosynthesis of secondary metabolites	01110	3.031e-25	%

Source	Gene names
Sma3	5'H; 5'h; AT4g12300; AT4g12310; At1g33720; At2g45550; At2g45570; At2g45580; At3g61040; At4g12300; At4g12300/T4C9_140; At4g12310; At5g07990; B1168A08.5; CYP706D2; CYP736A4v1; CYP736A5v1; CYP75; CYP75A1; CYP75A12\|F3'5'H1; CYP75A13; CYP75A2; CYP75A3; CYP75A5

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	microsome	GO:0005792	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	GO:0016702	Molecular Function	-
Sma3	flavonoid 3'-monooxygenase activity	GO:0016711	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	flavonoid 3',5'-hydroxylase activity	GO:0033772	Molecular Function	-
Sma3		GO:0050381	Molecular Function	-
Sma3	N-methylcoclaurine 3'-monooxygenase activity	GO:0050593	Molecular Function	-
Sma3	flavonoid biosynthetic process	GO:0009813	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Protein of unknown function DUF247, plant	IPR004158	-	-
Sma3	Lipoxygenase, C-terminal	IPR013819	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G45580.1	CYP76C3 cytochrome P450, family 76, subfamily C, polypeptide 3 chr2:18782388-18784286 REVERSE LENGTH=515	50%
RefSeq	Arabidopsis thaliana	NP_182082.2	cytochrome P450 76C3 [Arabidopsis thaliana]	50%
RefSeq	Populus trichocarpa	XP_002299579.1	cytochrome P450 [Populus trichocarpa]	53%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPD9

Fln msg: Overlapping hits, possible frame ERROR between 895 and 879,

Fln protein: MQFSPSSLLCTVAGLGCIFSTSMDIASLLFYSLLGITSAIIYFVLDVWKRKTYCCRLPPGPPGWPILGNLFQLGKNPHESLFRLASKYGPLMSLSLGMKTTVVVSSPPMAKEVLKTHGHIFAGRIVPQAGKALSHHKHSFLLCQYGSRWRMLRRISNNELFSVKRLEALQDLRRDQAHRMIHQIFEGSLKGSECVNIGHIAFHSAFNLIGNMAFGKHMFDSNLRASEDLKDAISRLMVLHTAPNLADYFPCLQFLDLQGVYRNMVICRKKSYAVMDKFIEDRxxxxxxxNNDISDDGKKDLLDVLLDMRNDEFTLPDIRGYLSDIFVAGSDTTAETIEWAMAELIRNPEKLKRAQAELEEVVGLNRRMEESDTERLPYLRAVVKEVFRLHPAGPLLVPHRANSDCEIGGFMIPKHSRVVVNVWGMGRDAQIWKEPLNFVPERFIDDEMCSHVDYRGKHFELIPFGAGTRMCVGLPLASRTVHLTLASLIHSFEWAPPKGTRAEELDMSEKFGLALQKAVPLEAIATPRLASHLY*

Protein Length: 536

Fln nts: ATGGGGTCAATGGGAAAGAGGAGGCATATGC_-_ATGCAGTTTTCTCCCTCTTCTCTGCTCTGTACAGTGGCAGGGCTCGGTTGCATCTTCTCGACCTCAATGGATATTGCAAGCTTATTATTCTATTCTCTGTTGGGCATCACCTCCGCCATCATATACTTTGTCTTGGACGTGTGGAAGAGAAAGACCTACTGCTGCAGGCTTCCTCCTGGACCTCCGGGATGGCCAATTTTGGGAAATCTGTTTCAGTTGGGTAAAAATCCCCACGAATCTCTGTTCCGCCTTGCCTCCAAATACGGCCCTCTCATGTCTCTTTCCCTCGGAATGAAAACCACAGTGGTGGTCTCCTCTCCCCCCATGGCAAAGGAAGTTCTCAAAACCCATGGCCACATCTTTGCAGGCCGAATTGTCCCTCAAGCAGGCAAAGCTCTTTCTCACCACAAGCACTCATTTCTTTTATGCCAATATGGATCTCGCTGGCGTATGCTCAGACGCATATCTAATAATGAACTCTTCTCTGTCAAGCGGCTAGAAGCTCTACAAGATCTCAGAAGAGATCAAGCACATCGAATGATTCATCAGATTTTTGAAGGCTCCCTGAAGGGATCAGAGTGTGTGAATATCGGACACATTGCATTCCACTCCGCGTTTAATCTGATAGGGAACATGGCCTTTGGTAAACACATGTTCGATTCTAACTTACGAGCATCTGAAGATCTGAAAGACGCTATTTCGAGACTGATGGTTCTACACACCGCTCCCAATTTGGCCGACTATTTCCCGTGTCTTCAGTTCTTGGATCTACAGGGTGTTTATCGTAATATGGTAATCTGCAGGAAAAAGTCGTATGCTGTTATGGATAAATTCATAGAGGATCGCxxxxxxxxxxxxxxxxxxxxxAACAACGACATAAGTGATGATGGAAAGAAGGATCTCTTGGACGTTTTGCTGGACATGAGAAATGATGAATTTACTCTCCCTGACATCAGGGGCTACTTAAGTGATATCTTTGTGGCTGGAAGTGATACGACCGCGGAGACCATAGAGTGGGCAATGGCAGAACTCATTCGCAATCCTGAGAAATTGAAACGAGCCCAAGCGGAGCTGGAAGAGGTTGTTGGTCTCAACCGCCGGATGGAAGAAAGTGATACCGAGCGTCTCCCTTATCTCCGCGCAGTGGTGAAAGAAGTGTTCCGATTGCACCCAGCAGGTCCTCTGCTCGTCCCACACAGAGCAAACAGCGATTGTGAGATCGGGGGGTTCATGATACCCAAACACAGCCGAGTAGTGGTGAACGTTTGGGGCATGGGAAGGGACGCTCAGATCTGGAAAGAGCCATTAAATTTCGTGCCAGAGAGGTTTATTGATGATGAGATGTGTAGTCATGTGGATTACAGAGGAAAACACTTTGAGCTGATACCGTTCGGAGCAGGAACGAGAATGTGCGTGGGGCTTCCATTGGCAAGTCGGACGGTTCATTTGACGTTGGCGTCGCTTATCCACTCATTTGAATGGGCACCGCCCAAGGGTACGCGTGCGGAGGAGTTAGACATGAGCGAGAAGTTTGGTCTGGCGCTCCAGAAGGCCGTACCTTTGGAAGCCATTGCCACGCCACGCCTTGCATCTCATTTGTATTAG___AATTTTACTTAGGGTGGTG

Fln Alignment:

Contig9601___MDIASLLFYSLLGITSAIIYFVLDVWKRK--TYCCRXXXXXXXXXXXXXXFQLGKNPHESLFRLASKYGPLMSLSLGMKTTVVVSSPPMAKEVLKTHGHIFAGRIVPQAGKALSHHKHSFLLCQYGSRWRMLRRISNNELFSVKRLEALQDLRRDQAHRMIHQIFEGSLKGSECVNIGHIAFHSAFNLIGNMAFGKHMFDSNLRASEDLKDAISRLMVLHTAPNLADYFPCLQFLDLQGVYRNMVICRKKSYAVMDKFIEDRLxxxxxKNNDISXXXXXXXXXXXXXMRNDEFTLPDIRGYLSDIFVAGSDTTAETIEWAMAELIRNPEKLKRAQAELEEVVGLNRRMEESDTERLPYLRAVVKEVFRLHPAGPLLVPHRANSDCEIGGFMIPKHSRVVVNVWGMGRDAQIWKEPLNFVPERFIDDEMCSHVDYRGKHFELIPFGAGTRMCVGLPLASRTVHLTLASLIHSFEWAPPKGTRAEELDMSEKFGLALQKAVPLEAIATPRLASHLY
B8LPD9______________MDIGRFVFYSLLGITSAIVYFVLDVWKKKKKTYC-RLPPGPQGWPIVGNLFQLGKKPHESLFRLATKYGPLMSLSLGMKTTVVVSSPSMAKEVLKTHGHVFAGRIVTQAARSLSHDKSSFLLCQYGSRWRTLRRISNTELFSVKRLDALQDLRRVQVRGMIHQIFENSVKGSGCVNIGHIAFHSAFNLIGNMAFGKDMFDPHFRASEDLKDAISKLMVLHTAPNLADYFPCLQFLDLQGVYRNTGIYRKKAYDVMDKFIEDRLxxxxxKNSDRTDDGKKDLLDVLLDMRSDEFTLTDIRGYLNDMFVAGSDTTAVTIEWAIAELVRNPEKLKRAQAELEEVIGLNRRLEESDTERLPYLRAVVKEVFRLHPAGPLLVPHRADGRFEIAGFVIPKHSRVLVNVWGMGRDPQIWNEPLKFVPERFIDDEMCGQMDYKGKDFELIPFGAGTRMCVGLPLASRMVHLVLGSLIHSFEWAPPKGMSAEQMDMTEKFGLALQKAVPLEAIATPRLLSHVY

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
971			25	75	0.996473
1019		25	75		0.996473
1041	20	80			0.995493
1086	25		75		0.996473
1095		75	25		0.996473
1102	14		85		0.993533
1105		85		14	0.993533
1151		66		33	1.0
1206	60		40		1.0
1221	50	50			1.0
1222	50		50		1.0
1244	50		50		1.0
1254		75		25	0.996473
1260			60	40	1.0
1264	60		40		1.0
1276		60		40	1.0
1293			60	40	1.0
1296		60		40	1.0
1318		25	75		0.996473
1321	25	75			0.996473
1398	75	25			0.996473
1401	66		33		0.997452
1402	66	33			0.997452
1425		83		16	0.994512
1449			60	40	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain