SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene9093

Length: 2563 nt

UniGene Fasta

>sp_v3.0_unigene9093
ATGGGGGTAACGAGACGAAGGGAAACCGCATTTATCCAGCAAGCGTGACTCTCCCGTTCTGAGACAATCGACGACAACGAACGAACGACAGAGGAAATCAGACAGAGCGGGCGGGGAGACTCTTTGTGGATATATCAAGGAGAAACGGAAACTATCTGAGATCTCTTTTCTGTGTGCAATGGCGGCAGCGGGGCTGGCCAAAGGCGGTGCGATCTCTGGGATAAAGATCGATGACAGAAATGTGCCGCTGCGTCTCGTGCCGCAGCGTCTCGTGCCGCATTCGGCTGCTTCGTCGTCCTCCTTGAGATTCCCAAGCTTGGAGGCGACGAATCGGTTTCCCCGTTCGAAAAGAAGGAATTCCGTGTCTTTGAGCGGGAAAGGACTGAGATTGGGGGCCGCAGAGGGGGAGATTATGGGGAGGAAAATGCATCTTTGGAGAACTGAAGGACCCGGGCGATCGCCGAAGCTCATAACTGTGACAGTCCGCATGGCTCTGTCCGAGATCCCACAAGAGCGCCTAGGATTATATGATCCCTCGCTTGATAAAGATGCTTGTGGAGTTGGCTTCGTTGCAGAGCTCTCAGGCAAAGCCAGTCGCAAAACTGTTACAGATGCAATTGAAATGCTGGTGCGCATGTCACATCGTGGTGCCTGTGGCTGCGAGACAAATACTGGTGATGGAGCTGGAATTCTCGTTGCTCTTCCTCATGAGTTCTTGGCTGAGGCTGTCAAGGATGCTGGATTTGATTTGCCGCCTCAAGAAGAGTATGCAGTTGGCATGTTCTTTTTACCAACCTCTGAAACTCGACGGGAGGAAAGCAAAAATGTGTTTAACAAGGTTGCAGAGTCACTGGGGCATGTAGTGCTGGGGTGGAGGCATGTCATAACTGACAATAATGGATTGGGTAAATCGGCTCTTCAAACAGAGCCTATTGTTGAACAAGTGTTTCTTACAAAAAGCTCTCGATCTAGTGCGGAGTTTGAGGAACAGATGTATATACTGCGGCGGACCTCAATGGTAGCCATCAGAGCTGCATTAAACCTGCAGCATGGTGGAGTAAGAGATTTCTACATATGCTCCCTCTCTTCCAGGACTATTGTATATAAAGGCCAACTCAAACCTGACCAATTGAAGAGCTACTACTTTGCGGATTTGGGAGATGACAAGTTTACTAGCTACATGGCTTTGATTCATTCACGGTTTTCAACAAACACATTTCCTAGCTGGGATCGTGCTCAGCCCATGCGCATATTAGGGCACAATGGGGAGATAAATACTCTGCGAGGAAATGTCAACTGGATGAAAGCACGGGAAGGATTATTAAAGTGCCAAAACCTTGCTCTTTCAAAGGATGAGATGCAGAAACTTCTTCCTATTGTGGATGCAAGCTCTTCAGATTCAGGGGCTTTCGATGGTGTATTAGAGCTTCTTGTTCGAGCGGGGAGGAGTCTTCCTGAAGCAGTAATGATGATGATTCCAGAGGCATGGCAGAATGACAAAAACATGGACCCTGATCGTAGAGCTTTATATGAATATTTCTCAGCTTTAATGGAACCATGGGATGGGCCAGCCCTTGTAGCATTCTCTGATGGTCGCTATCTTGGAGCAACTCTAGACCGTAATGGACTACGTCCTGGCCGTTTCTATGTAACCTACAGTGGGCGAGTTATCATGGCAAGTGAAGTAGGTGTTGTAGACATTCCACCTGAGGATGTTTGCCAAAAGGGAAGGCTTAATCCTGGCATGATGCTACTTGTCGATTTTGAAAAACATGGTATTGTGGATGATGAGGCCTTAAAAAAACAATATTCCATGTCACGGCCCTATTCAGAATGGATAAGGAGACAGAAGATTACCCTCAAAGATATTGTCTCTTCTGTTCCAGAAGCAGAAAGAGTGCCTGAACAAGTTATTGGATCACTAAAGGTTGATGGTTCGGATGATACAATGGAAAACATGGGAATATATGGCTTGTTGTCACCATTGAAGGTGTATGGGTATACAGTTGAGGCTTTGGAAATGTTATTGCTTCCTATGGCAAAATATGGAACTGAATCCCTTGGTTCCATGGGGAATGATACACCTTTAGCTGTGATGTCAAATAGACCGAAAATAATATTCGAGTACTTCAAGCAGATGTTTGCTCAAGTGACAAACCCTCCTATTGATCCAATTCGTGAAGCGATTGTCACCTCAATGGAATGTATGATTGGTCCAGAAGGGGATCTTACGGAAACTAGTGAGAAGCAGTGCCATCGGCTTTCTCTTAAAGGTCCTATTCTTTCTATTGAGGAAACAGAAGCAATTAAAAAATGAATTACCGAGGTTGGCGTAGCAAAGTGCTTGATATCACATTTCCAAAAGAACGTGGCTCTAGAGGATTGGAAGAGACCTTGGATAGGATATGCTCGGAGGCTCGTCTTGCAATTCGTGAAGGTTACAAATTAATAGTGCTATCAGATAGAGCTACATCATCCAAGCGGGTTCCTGTTAGCTCTCTGTTAGCTGTTGGTGCTGTGCATCACCATCTTGCTCAGAGTTAGAACGACTCGAATTGGATTG

Ace file of the UniGene sp_v3.0_unigene9093

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	glutamate synthase 1 [NADH] [Arabidopsis thaliana] ref\|NP_001190529.1\| glutamate synthase 1 [NADH] [Arabidopsis thaliana] ref\|NP_001190530.1\| glutamate synthase 1 [NADH] [Arabidopsis thaliana] sp\|Q9LV03.2\|GLUT1_ARATH RecName: Full=Glutamate synthase 1 [NA	-	-	0.0	77%
FL-Next	sp=Glutamate synthase 1 [NADH], chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	79%
Sma3	NADH glutamate synthase	-	-	6.97e-11	-

Source	ECs	Term	Type	e value	Identity
Sma3	Glutamate synthase (NADH).	EC:1.4.1.14	-	7.669e-26	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Alanine, aspartate and glutamate metabolism	00250		7.669e-26	%
Sma3	Nitrogen metabolism	00910		7.669e-26	%
Sma3	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064		7.669e-26	%
Sma3	Metabolic pathways	01100		7.669e-26	%
Sma3	Biosynthesis of secondary metabolites	01110		7.669e-26	%
Sma3	Glutamate synthase (ferredoxin).	EC:1.4.7.1	-	3.281e-11	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Nitrogen metabolism	00910		3.281e-11	%

Source	Gene names
Sma3	CHLREDRAFT_205746; Fd-GOGAT; GLSF; GLT; GLT2; GSN1; GSVIVT00021931001; GSVIVT00022939001; GSVIVT00028012001; GSVIVT00031545001; GSVIVT00037107001; GltX; MICPUCDRAFT_57115; MICPUN_105473; NADH-GOGAT; NADH-GOGAT.1; NADH-GOGAT.2; NGS; OJ1263_E10.17; Os01g068

Source	GOs	Term	Type	Identity
Sma3	amyloplast	GO:0009501	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	glycine hydroxymethyltransferase activity	GO:0004372	Molecular Function	-
Sma3	protein kinase activity	GO:0004672	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	FMN binding	GO:0010181	Molecular Function	-
Sma3	glutamate synthase activity	GO:0015930	Molecular Function	-
Sma3	glutamate synthase (NADH) activity	GO:0016040	Molecular Function	-
Sma3	glutamate synthase (ferredoxin) activity	GO:0016041	Molecular Function	-
Sma3	carbon-nitrogen ligase activity, with glutamine as amido-N-donor	GO:0016884	Molecular Function	-
Sma3	glutamate synthase activity, NADH or NADPH as acceptor	GO:0045181	Molecular Function	-
Sma3	flavin adenine dinucleotide binding	GO:0050660	Molecular Function	-
Sma3	iron-sulfur cluster binding	GO:0051536	Molecular Function	-
Sma3	3 iron, 4 sulfur cluster binding	GO:0051538	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	glutamate biosynthetic process	GO:0006537	Biological Process	-
Sma3	glutamine metabolic process	GO:0006541	Biological Process	-
Sma3	glycine metabolic process	GO:0006544	Biological Process	-
Sma3	L-serine metabolic process	GO:0006563	Biological Process	-
Sma3	nitrogen compound metabolic process	GO:0006807	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Amidase	IPR000120	-	-
Sma3	Glutamine amidotransferase, class-II	IPR000583	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3		IPR000759	-	-
Sma3	Serine hydroxymethyltransferase	IPR001085	-	-
Sma3	Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain	IPR001327	-	-
Sma3	Ankyrin repeat	IPR002110	-	-
Sma3	Glutamate synthase, alpha subunit, C-terminal	IPR002489	-	-
Sma3	Glutamate synthase, central-C	IPR002932	-	-
Sma3	Glutamate synthase, NADH/NADPH, small subunit 1	IPR006005	-	-
Sma3	Glutamate synthase, central-N	IPR006982	-	-
Sma3	Glutamate synthase, eukaryotic	IPR012220	-	-
Sma3	Fumarate reductase, C-terminal	IPR012285	-	-
Sma3	FAD-dependent pyridine nucleotide-disulphide oxidoreductase	IPR013027	-	-
Sma3	Aldolase-type TIM barrel	IPR013785	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3		IPR017442	-	-
Sma3	Glutamine amidotransferase, type II	IPR017932	-	-
Sma3	Rhodopsin, retinal binding site	IPR018229	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G53460.1	GLT1 NADH-dependent glutamate synthase 1 chr5:21700518-21709629 FORWARD LENGTH=2208	79%
RefSeq	Arabidopsis thaliana	NP_200158.2	glutamate synthase 1 [NADH] [Arabidopsis thaliana]	79%
RefSeq	Populus trichocarpa	XP_002321436.1	predicted protein [Populus trichocarpa]	78%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: sp_plants

Fln subject: Q9LV03

Fln msg: Separated hits, possible frame ERROR between 2284 and 2311, Unexpected STOP codon at 3' end. Distance to subject end: 1422 aas, your sequence is shorter than subject: 789 - 2208

Fln protein: MAAAGLAKGGAISGIKIDDRNVPLRLVPQRLVPHSAASSSSLRFPSLEATNRFPRSKRRNSVSLSGKGLRLGAAEGEIMGRKMHLWRTEGPGRSPKLITVTVRMALSEIPQERLGLYDPSLDKDACGVGFVAELSGKASRKTVTDAIEMLVRMSHRGACGCETNTGDGAGILVALPHEFLAEAVKDAGFDLPPQEEYAVGMFFLPTSETRREESKNVFNKVAESLGHVVLGWRHVITDNNGLGKSALQTEPIVEQVFLTKSSRSSAEFEEQMYILRRTSMVAIRAALNLQHGGVRDFYICSLSSRTIVYKGQLKPDQLKSYYFADLGDDKFTSYMALIHSRFSTNTFPSWDRAQPMRILGHNGEINTLRGNVNWMKAREGLLKCQNLALSKDEMQKLLPIVDASSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNMDPDRRALYEYFSALMEPWDGPALVAFSDGRYLGATLDRNGLRPGRFYVTYSGRVIMASEVGVVDIPPEDVCQKGRLNPGMMLLVDFEKHGIVDDEALKKQYSMSRPYSEWIRRQKITLKDIVSSVPEAERVPEQVIGSLKVDGSDDTMENMGIYGLLSPLKVYGYTVEALEMLLLPMAKYGTESLGSMGNDTPLAVMSNRPKIIFEYFKQMFAQVTNPPIDPIREAIVTSMECMIGPEGDLTETSEKQCHRLSLKGPIxxxxxxxxxxKMNYRGWRSKVLDITFPKERGSRGLEETLDRICSEARLAIREGYKLIVLSDRATSSKRVPVSSLLAVGAVHHHLAQS*

Protein Length: 790

Fln nts: ATGGGGGTAACGAGACGAAGGGAAACCGCATTTATCCAGCAAGCGTGACTCTCCCGTTCTGAGACAATCGACGACAACGAACGAACGACAGAGGAAATCAGACAGAGCGGGCGGGGAGACTCTTTGTGGATATATCAAGGAGAAACGGAAACTATCTGAGATCTCTTTTCTGTGTGCA_-_ATGGCGGCAGCGGGGCTGGCCAAAGGCGGTGCGATCTCTGGGATAAAGATCGATGACAGAAATGTGCCGCTGCGTCTCGTGCCGCAGCGTCTCGTGCCGCATTCGGCTGCTTCGTCGTCCTCCTTGAGATTCCCAAGCTTGGAGGCGACGAATCGGTTTCCCCGTTCGAAAAGAAGGAATTCCGTGTCTTTGAGCGGGAAAGGACTGAGATTGGGGGCCGCAGAGGGGGAGATTATGGGGAGGAAAATGCATCTTTGGAGAACTGAAGGACCCGGGCGATCGCCGAAGCTCATAACTGTGACAGTCCGCATGGCTCTGTCCGAGATCCCACAAGAGCGCCTAGGATTATATGATCCCTCGCTTGATAAAGATGCTTGTGGAGTTGGCTTCGTTGCAGAGCTCTCAGGCAAAGCCAGTCGCAAAACTGTTACAGATGCAATTGAAATGCTGGTGCGCATGTCACATCGTGGTGCCTGTGGCTGCGAGACAAATACTGGTGATGGAGCTGGAATTCTCGTTGCTCTTCCTCATGAGTTCTTGGCTGAGGCTGTCAAGGATGCTGGATTTGATTTGCCGCCTCAAGAAGAGTATGCAGTTGGCATGTTCTTTTTACCAACCTCTGAAACTCGACGGGAGGAAAGCAAAAATGTGTTTAACAAGGTTGCAGAGTCACTGGGGCATGTAGTGCTGGGGTGGAGGCATGTCATAACTGACAATAATGGATTGGGTAAATCGGCTCTTCAAACAGAGCCTATTGTTGAACAAGTGTTTCTTACAAAAAGCTCTCGATCTAGTGCGGAGTTTGAGGAACAGATGTATATACTGCGGCGGACCTCAATGGTAGCCATCAGAGCTGCATTAAACCTGCAGCATGGTGGAGTAAGAGATTTCTACATATGCTCCCTCTCTTCCAGGACTATTGTATATAAAGGCCAACTCAAACCTGACCAATTGAAGAGCTACTACTTTGCGGATTTGGGAGATGACAAGTTTACTAGCTACATGGCTTTGATTCATTCACGGTTTTCAACAAACACATTTCCTAGCTGGGATCGTGCTCAGCCCATGCGCATATTAGGGCACAATGGGGAGATAAATACTCTGCGAGGAAATGTCAACTGGATGAAAGCACGGGAAGGATTATTAAAGTGCCAAAACCTTGCTCTTTCAAAGGATGAGATGCAGAAACTTCTTCCTATTGTGGATGCAAGCTCTTCAGATTCAGGGGCTTTCGATGGTGTATTAGAGCTTCTTGTTCGAGCGGGGAGGAGTCTTCCTGAAGCAGTAATGATGATGATTCCAGAGGCATGGCAGAATGACAAAAACATGGACCCTGATCGTAGAGCTTTATATGAATATTTCTCAGCTTTAATGGAACCATGGGATGGGCCAGCCCTTGTAGCATTCTCTGATGGTCGCTATCTTGGAGCAACTCTAGACCGTAATGGACTACGTCCTGGCCGTTTCTATGTAACCTACAGTGGGCGAGTTATCATGGCAAGTGAAGTAGGTGTTGTAGACATTCCACCTGAGGATGTTTGCCAAAAGGGAAGGCTTAATCCTGGCATGATGCTACTTGTCGATTTTGAAAAACATGGTATTGTGGATGATGAGGCCTTAAAAAAACAATATTCCATGTCACGGCCCTATTCAGAATGGATAAGGAGACAGAAGATTACCCTCAAAGATATTGTCTCTTCTGTTCCAGAAGCAGAAAGAGTGCCTGAACAAGTTATTGGATCACTAAAGGTTGATGGTTCGGATGATACAATGGAAAACATGGGAATATATGGCTTGTTGTCACCATTGAAGGTGTATGGGTATACAGTTGAGGCTTTGGAAATGTTATTGCTTCCTATGGCAAAATATGGAACTGAATCCCTTGGTTCCATGGGGAATGATACACCTTTAGCTGTGATGTCAAATAGACCGAAAATAATATTCGAGTACTTCAAGCAGATGTTTGCTCAAGTGACAAACCCTCCTATTGATCCAATTCGTGAAGCGATTGTCACCTCAATGGAATGTATGATTGGTCCAGAAGGGGATCTTACGGAAACTAGTGAGAAGCAGTGCCATCGGCTTTCTCTTAAAGGTCCTATTCTxxxxxxxxxxxxxxxxxxxxxxxxxxxAAAAATGAATTACCGAGGTTGGCGTAGCAAAGTGCTTGATATCACATTTCCAAAAGAACGTGGCTCTAGAGGATTGGAAGAGACCTTGGATAGGATATGCTCGGAGGCTCGTCTTGCAATTCGTGAAGGTTACAAATTAATAGTGCTATCAGATAGAGCTACATCATCCAAGCGGGTTCCTGTTAGCTCTCTGTTAGCTGTTGGTGCTGTGCATCACCATCTTGCTCAGAGTTAGAACGACTCGAATTGGATTG

Fln Alignment:

Contig9093___MHLWRTEGPGRSPKLITVTVRMALSEIPQERLGLYDPSLDKDACGVGFVAELSGKASRKTVTDAIEMLVRMSHRGACGCETNTGDGAGILVALPHEFLAEAVKDAGFDLPPQEEYAVGMFFLPTSETRREESKNVFNKVAESLGHVVLGWRHVITDNNGLGKSALQTEPIVEQVFLTKSSRSSAEFEEQMYILRRTSMVAIRAALNLQHGGVRDFYICSLSSRTIVYKGQLKPDQLKSYYFADLGDDKFTSYMALIHSRFSTNTFPSWDRAQPMRILGHNGEINTLRGNVNWMKAREGLLKCQNLALSKDEMQKLLPIVXXXXXXXXXXXXVLELLVRAGRSLPEAVMMMIPEAWQNDKNMDPDRRALYEYFSALMEPWDGPALVAFSDGRYLGATLDRNGLRPGRFYVTYSGRVIMASEVGVVDIPPEDVCQKGRLNPGMMLLVDFEKHGIVDDEALKKQYSMSRPYSEWIRRQKITLKDIVSSVPEAERVPEQVIGSLKVDGSDDTMENMGIYGLLSPLKVYGYTVEALEMLLLPMAKYGTESLGSMGNDTPLAVMSNRPKIIFEYFKQMFAQVTNPPIDPIREAIVTSMECMIGPEGDLTETSEKQCHRLSLKGPILxxxxxxxxxKMNYRGWRSKVLDITFPKERGSRGLEETLDRICSEARLAIREGYKLIVLSDRATSSKRVPVSSLLAVGAVHHHLAQS
Q9LV03______________LQFWRSDGPGRSAKLRTV-VKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELGFVLPSAGNYAVGMFFLPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTKSKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLxxxxxxxxxKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKT

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
33			33	66	1.0
34			33	66	1.0
56		87		12	0.992554
78	62		37		1.0
254		11		88	0.923564
1608			10	90	0.90298
1609			10	90	0.90298
1640		85		14	0.999981
1738		10	90		0.90298
1739	10	90			0.90298
2129		33		66	1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
76	88	AACG AACG AACG A	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain