SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene8725

Length: 1924 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene8725

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Predicted protein n=11 Tax=Physcomitrella patens RepID=A9S6L2_PHYPA	-	-	0.0	86%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	86%
Sma3	Glycogen synthase kinase-3 beta, putative	-	-	1.767e-16	-

Source	ECs	Term	Type	e value	Identity
Sma3	Non-specific serine/threonine protein kinase.	EC:2.7.11.1	-	2.957e-15	-
Sma3	[Tau protein] kinase.	EC:2.7.11.26	-	5.878e-14	-

Source	Gene names
Sma3	ASK1; ASK10; ASK2; ASK3; ASK4; ASK5; ASK6; ASK7; ASK8; ASK9; AT1G06390; AT1G09840; AT3G05840; AT4G18710; At1g06390; At1g09840; At1g57870; At2g30980; At3g05840; At3g61160; At4g00720; At4g18710; At5g14640; At5g26751; BIN2; CDK-A; CDKA; CHLREDRAFT_114692; DW

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	protein kinase activity	GO:0004672	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	brassinosteroid mediated signaling pathway	GO:0009742	Biological Process	-
Sma3	regulation of protein localization	GO:0032880	Biological Process	-
Sma3	hyperosmotic salinity response	GO:0042538	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Immunoglobulin/major histocompatibility complex, conserved site	IPR003006	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Zinc finger, RING-CH-type	IPR011016	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Zinc finger, C3HC4 RING-type	IPR018957	-	-
Sma3	Peroxidases heam-ligand binding site	IPR019793	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G14640.1	ATSK13, SK13 shaggy-like kinase 13 chr5:4719350-4721772 REVERSE LENGTH=410	84%
RefSeq	Arabidopsis thaliana	NP_196968.2	Shaggy-related protein kinase epsilon [Arabidopsis thaliana]	84%
RefSeq	Populus trichocarpa	XP_002320270.1	predicted protein [Populus trichocarpa]	83%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNT6

Fln msg: Overlapping hits, possible frame ERROR between 578 and 571, atg_distance in limit (1-15): atg_distance = 5,

Fln protein: MKKVKNEPVIAPREVYKDSIAEVSVELSKKMIKNNINVGSANEVCPLTYRDNAIPREEIVPDTLDDLPKEMKELKIRDDKSSNADIKETDVPIVDGIVTEVGQVIAMTVGGRNGQLKQTVSYKAERVVGTGSFGTVFQAKCLETGETVAIKKVLQDKRYKNRELQMMRLMEHPNIVSLKHCxxxxTTDKDELYLNLVLEYVPETVYRITKHYTRINQRMPLLYVKLYTYQMCRALAYTHNSIGVCHRDIKPQNLLVNPHSHQLKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTAIDVWSTGCVMAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYPEFKFPQIKAHPWQKLFHRRAPAEAIDLLSRILQYSPSLRCTALEACAHPFFDELRDPSTCLPNGRPLPPLFNFKPHELKGATPDLIQKLIPDHARK*

Protein Length: 454

Fln nts: TGAAAAGGTCAACAGATACAACCAAC_-_ATGAAAAAGGTGAAAAATGAGCCAGTTATTGCTCCCAGAGAGGTGTACAAGGATTCTATTGCTGAAGTTTCAGTAGAGCTTTCTAAGAAAATGATAAAGAACAATATTAATGTGGGCTCTGCCAACGAAGTTTGCCCTCTGACTTATCGTGACAATGCTATTCCACGAGAAGAAATAGTTCCGGATACGTTAGATGACCTCCCAAAGGAAATGAAGGAGCTGAAGATCAGGGATGACAAAAGCAGCAATGCTGATATTAAGGAAACAGATGTTCCTATAGTAGATGGAATTGTCACCGAGGTTGGACAGGTTATTGCGATGACGGTTGGTGGTAGAAATGGCCAGCTGAAGCAAACTGTCAGTTATAAGGCAGAGCGGGTTGTGGGAACTGGGTCTTTTGGCACTGTCTTTCAGGCAAAATGCTTAGAAACTGGTGAGACTGTAGCTATCAAAAAGGTCCTGCAGGACAAACGATATAAAAATCGTGAATTGCAAATGATGCGCCTTATGGAGCACCCCAATATTGTATCTCTGAAACACTGTxxxxxxxxxxxxACCACTGACAAAGATGAACTGTACCTCAATTTGGTTCTTGAGTATGTACCAGAGACAGTTTATCGAATCACAAAGCACTACACTAGAATCAATCAGCGGATGCCTTTACTTTATGTAAAACTATACACTTATCAGATGTGCAGAGCACTAGCTTATACTCATAATTCGATTGGGGTATGTCACAGAGACATCAAGCCGCAGAACCTTTTGGTCAATCCCCACTCGCACCAGCTCAAGTTATGTGATTTTGGAAGTGCCAAAGTTCTGGTCAAGGGTGAACCGAATATATCATATATTTGCTCCCGCTATTATCGAGCTCCTGAGCTGATCTTTGGAGCTACAGAGTATACAACTGCTATTGATGTATGGTCAACAGGTTGTGTCATGGCTGAGCTACTTTTGGGTCAGCCACTTTTCCCTGGGGAGAGTGGAGTGGACCAGCTTGTGGAGATTATTAAGGTCCTTGGGACTCCCACTCGCGAGGAGATTAAATGCATGAATCCTAATTATCCAGAGTTTAAGTTTCCACAGATAAAGGCCCACCCTTGGCAGAAGCTTTTCCACAGGCGAGCACCTGCAGAGGCGATAGATCTTCTCTCAAGGATTCTCCAGTACTCACCGAGTCTTCGCTGCACTGCTCTTGAAGCATGTGCACATCCCTTCTTTGATGAACTCCGGGATCCCAGCACCTGCTTACCTAATGGTCGCCCCTTACCCCCACTGTTTAACTTCAAACCACATGAACTAAAAGGAGCTACTCCTGACCTTATTCAAAAGTTGATCCCTGATCATGCAAGAAAGTGA___TTGCTTGGTTTTGAATTGAAAGAGAGAAAAATGCATATTTGATGGAATCAGCGGCATTGATTACTGTGTTCGGGGCTTCAAAATTTCAGGAAAAATATCTAATGCTGTGTCTTGTAAGTTATGTGGTAAAAGTCCTGCAATTGATGGGCAATATTCTTGCAAAAAATATTCACTAATTGTGGGGCACAATTCTTTTCTATATTCTCTCACCTGTTGGGAACCTAAGATATCCTCTTTAAACTGTTGAAAATGATTTCAAGATGAAAGGCACATTTTGAAAATTCATGAGGTCGGGGCTGAACATCAAAACTGCTTATAGACCATGGTTTCTAAAATCTATCAGCATGTACGATCATAATTATTTGTCATTTCTTTGTATTCTAGTTGATTCTTACAAGTTCTTATCCCGCAAAGAATATTTTAGGCAAGAATGCTATCCAGGACATGTTCGTGAATCATATTTTCTTCTCAAATGTCAAGACTTGTAAGAAACATTAAGTCCAGCTACTATATCTATGTTTATCTCTTATTGTTTCCTTGC

Fln Alignment:

Contig8725___STDTTNMKKVKNE----PVIAPREVYKDSIAEVSVELSKKMIKNNINVGSANEVCPLTYRDNAIPREEIVPDTLDDLPKEMKELKIRDDKSSNADIKETDVPIVDGIVTEVGQVIAMTVGGRNGQLKQTVSYKAERVVGTGSFGTVFQAKCLETGETVAIKKVLQDKRYKNRELQMMRLMEHPNIVSLKHCxxxTTDKDELYLNLVLEYVPETVYRITKHYTRINQRMPLLYVKLYTYQMCRALAYTHNSIGVCHRDIKPQNLLVNPHSHQLKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTAIDVWSTGCVMAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYPEFKFPQIKAHPWQKLFHRRAPAEAIDLLSRILQYSPSLRCTALEACAHPFFDELRDPSTCLPNGRPLPPLFNFKPHELKGATPDLIQKLIPDHARK
B8LNT6______________STDTTNMKKVKNEQLTEPVNASKEVYKDSIAEVSVEVSKKMTKNKSNVGSADEGPPLTYRGNALRQKEIVPDTLDDLPKEMKELKIRDDKRSNLDNKEIDVPIVDGIVTEIGQIITMTVGGKNGQLKQTVSYKAERVVGTGSFGTVFQAKCLETGETVAIKKVLQDKRYKNRELQMMRLMEHPNIVSLKHCxxxTTDKDELYLNLVLEYVPETVYRITKHYTRISQRMPLLYVKLYTYQMCRALAYTHGAIGVCHRDIKPQNLLVNPHSHQLKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTAIDVWSTGCVMAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQIKAHPWQKLFHKRAPAEAVDLLSRILQYSPNLRCNALEACAHPFFDELRDSSTCLPNGRPLPPLFNFKPHELKGATPELLQKLIPDHARK

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
106		33		66	0.997452
349		83		16	0.994512
405	20		80		0.995493
414	83			16	0.994512
415		83	16		0.994512
416			16	83	0.994512
473		16		83	0.994512
496	80			20	0.995493
497	75			25	0.996473
734	88		11		0.923564
822		12		87	0.992554
1056	7			92	0.999602
1140		86		9	0.999642
1365		11		88	0.999952
1398	83		16		1.0
1414	89		10		0.999919
1415		21		78	1.0
1445	11			88	0.999937
1789		88	11		0.923564
1790		88	11		0.923564

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain