SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene8645

Length: 1784 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene8645

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Geraniol 10-hydroxylase n=1 Tax=Catharanthus roseus RepID=Q8VWZ7_CATRO	-	-	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	83%
Sma3	Flavonoid 3'-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3		EC:1.14.-.-	-	5.656e-07	-
Sma3	Flavonoid 3'-monooxygenase.	EC:1.14.13.21	-	6.582e-07	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Flavonoid biosynthesis	00941		6.582e-07	%
Sma3	Flavone and flavonol biosynthesis	00944		6.582e-07	%
Sma3	Biosynthesis of phenylpropanoids	01061		6.582e-07	%
Sma3	Metabolic pathways	01100		6.582e-07	%
Sma3	Biosynthesis of secondary metabolites	01110		6.582e-07	%
Sma3	N-methylcoclaurine 3'-monooxygenase.	EC:1.14.13.71	-	6.46e-16	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Isoquinoline alkaloid biosynthesis	00950		6.46e-16	%
Sma3	Metabolic pathways	01100		6.46e-16	%
Sma3	Biosynthesis of secondary metabolites	01110		6.46e-16	%
Sma3	Flavonoid 3',5'-hydroxylase.	EC:1.14.13.88	-	8.089e-16	-

Source	KEGGs	Term	e value	Identity
Sma3	Flavonoid biosynthesis	00941	8.089e-16	%
Sma3	Flavone and flavonol biosynthesis	00944	8.089e-16	%
Sma3	Biosynthesis of secondary metabolites	01110	8.089e-16	%

Source	Gene names
Sma3	5'H; AT4g12300; AT4g12310; At1g33720; At2g45550; At2g45560; At2g45570; At2g45580; At3g52970/F8J2_140; At3g61040; At4g12300; At4g12300/T4C9_140; At4g12310; At5g07990; B1168A08.5; CYP706C5; CYP706D1; CYP706D2; CYP736A5v1; CYP75; CYP75B1; CYP75B2; CYP76A8; C

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	microsome	GO:0005792	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	sequence-specific DNA binding transcription factor activity	GO:0003700	Molecular Function	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	RNA-directed DNA polymerase activity	GO:0003964	Molecular Function	-
Sma3	aspartic-type endopeptidase activity	GO:0004190	Molecular Function	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	flavonoid 3'-monooxygenase activity	GO:0016711	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3		GO:0050381	Molecular Function	-
Sma3	N-methylcoclaurine 3'-monooxygenase activity	GO:0050593	Molecular Function	-
Sma3	RNA-dependent DNA replication	GO:0006278	Biological Process	-
Sma3	proteolysis	GO:0006508	Biological Process	-
Sma3	flavonoid biosynthetic process	GO:0009813	Biological Process	-
Sma3		GO:0045449	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Reverse transcriptase	IPR000477	-	-
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Protein of unknown function DUF247, plant	IPR004158	-	-
Sma3	Lipoxygenase, C-terminal	IPR013819	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G45570.1	CYP76C2 cytochrome P450, family 76, subfamily C, polypeptide 2 chr2:18779935-18781922 REVERSE LENGTH=512	45%
RefSeq	Arabidopsis thaliana	NP_182081.1	cytochrome P450 76C2 [Arabidopsis thaliana]	45%
RefSeq	Populus trichocarpa	XP_002299579.1	cytochrome P450 [Populus trichocarpa]	47%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A813

Fln msg: your sequence is longer than subject: 475 - 321

Fln protein: MAHCWESSPARGKPHESMYRLAAKYGPLMTLQLGMRTTVVVSSPAMAKEVLKNHDQIFSGRTVIEAAKCLSYSDTSLVWNNCGPRWRMLRKFCNTELFSVKRLEALQHLRRDQVFSTIQSIFEDSITAKGVNLGHTVFLTSLNLLGNLIFSQNMFGRDSQAAEEFKETVTKLMVIGGKPNLADYFPFLRISDPQGVSRDVSKNIAIVFELLDRSVEARLQSRREGRTDDSGGKAKDFLDILLEYKSESGETFTKKDIIPFLYDMFVAGSETSSATIEWAMAEAIRNPRIMKKAQAELDEVIGTGRRVEETDIDRLPYLHAVVKETFRLHPPAPLLIPHRAESSCEVAGYMIPKDTQLLVNAWAIGRDPTIWDEPTKFKPERFVESEVEYRGQNFELIPFGVGRRICPGLPLAHRMVHVVTASLLHSFNWSLPDGITTDTMDMSERFGITLQRASPLIAIPSLRLPSHLFNDQHH*

Protein Length: 476

Fln nts: ACTTGGGTTCTTAGTCTCGTGATTTCTCTGAAGTCTAAACCCCATTGTGTTTTTGGGTTTGCTGGAGAGCACTTGAGCTATGGAGGAAATTAGTGGTTTCTGGTACTATGTCATGATTTTCATGACATTATGGTTGGTTTTAAAGGTGATTGATATGTCAAGAAAGGGCGCAGGCAAGCTGCCTCCAGGGCCTCCGGG_-_ATGGCCCATTGTTGGGAATCTTCTCCAGCTCGGGGGAAGCCCCACGAGTCTATGTATCGCCTTGCTGCAAAATACGGTCCTCTGATGACCCTTCAACTCGGAATGAGAACCACAGTTGTGGTTTCATCTCCTGCCATGGCGAAGGAAGTATTGAAGAATCATGACCAGATCTTTTCTGGACGCACTGTTATAGAAGCGGCCAAATGCCTCTCCTACAGCGACACTTCTTTGGTATGGAACAACTGCGGGCCTCGGTGGCGTATGCTTCGAAAATTCTGCAACACCGAGCTGTTTAGTGTGAAGAGACTGGAGGCCTTGCAGCATCTCAGAAGAGATCAGGTCTTTTCCACCATTCAATCCATTTTTGAGGATTCTATAACGGCGAAGGGCGTGAATCTTGGGCACACTGTGTTTCTTACCAGTCTGAATCTGTTGGGCAACTTGATTTTTTCTCAAAATATGTTCGGACGGGACTCTCAGGCCGCTGAAGAATTCAAAGAAACGGTCACCAAATTAATGGTAATTGGGGGAAAGCCGAACCTGGCTGACTACTTTCCTTTTCTCCGAATTTCGGACCCTCAGGGGGTGAGCCGCGATGTAAGCAAGAATATCGCCATTGTATTTGAGCTGCTGGATCGATCCGTAGAGGCTCGCCTCCAGAGTAGAAGAGAGGGCAGAACTGATGACAGCGGTGGCAAGGCAAAAGACTTTCTGGACATTTTACTTGAATACAAAAGCGAGAGCGGAGAAACTTTCACCAAGAAAGACATTATCCCCTTTCTATATGACATGTTCGTGGCGGGTAGCGAAACGAGCAGTGCCACAATTGAATGGGCAATGGCAGAGGCAATTCGAAATCCTCGCATAATGAAGAAAGCTCAAGCAGAATTGGATGAAGTAATCGGGACAGGTAGAAGAGTTGAAGAAACTGACATCGATCGCCTGCCTTATCTTCATGCAGTTGTGAAAGAAACGTTTCGTTTGCACCCGCCAGCGCCTCTGCTGATCCCTCACAGAGCAGAGAGCAGTTGTGAAGTAGCAGGGTACATGATACCCAAGGACACTCAGCTGCTGGTAAATGCTTGGGCCATAGGAAGAGACCCAACCATCTGGGATGAACCCACAAAGTTCAAGCCTGAGAGATTTGTGGAGTCTGAGGTGGAATACAGGGGGCAAAACTTCGAACTGATTCCGTTTGGAGTAGGAAGAAGAATTTGCCCTGGGCTTCCTCTGGCTCATAGGATGGTTCACGTGGTCACAGCTTCTCTCCTCCACTCATTCAACTGGTCTCTCCCGGATGGCATCACAACAGATACCATGGACATGAGTGAGAGATTTGGAATCACCCTGCAGAGAGCTTCTCCATTAATTGCAATCCCCTCACTGCGCTTGCCCTCTCATCTCTTTAACGATCAGCATCACTAG___TCAAACTTCTTCTGTCAACCAATCCTCTACCTAAATATCATCCTTTCCTTGCATGTAGATGTATGTATGGATGTCAGACCTTTCTTGCATATCATCATTTCCTTGCATGGAGATGTATGTATGTATGTCAGGCCTTTCTTGCATAATATAATATAATAGGA

Fln Alignment:

Contig8645___MFGRDSQAAEEFKETVTKLMVIGGKPNLADYFPFLRISDPQGVSRDVSKNIAIVFELLDRSVEARLQSRREGRTDDSGGKAKDFLDILLEYKSESGETFTKKDIIPFLYDMFVAGSETSSATIEWAMAEAIRNPRIMKKAQAELDEVIGTGRRVEETDIDRLPYLHAVVKETFRLHPPAPLLIPHRAESSCEVAGYMIPKDTQLLVNAWAIGRDPTIWDEPTKFKPERFVESEVEYRGQNFELIPFGVGRRICPGLPLAHRMVHVVTASLLHSFNWSLPDGITTDTMDMSERFGITLQRASPLIAIPSLRLPSHLFNDQHH
D5A813______________MFGRDSQTAEEFKEAVTKLMVIGGKPNLVNYFPFLRILDPQGVSRDVIKYMGIVFGLLDRSVEARLQRRKEGRTEHSDAKAKDFLDILLDYRSESGERFTKKDIIPFLYDLLIAGTETNSSTIEWTIAEAIRNPRIMKKAQAELEEVVGKDRRVEESDIDRLPYLHAVVREVFRLHPPVPLLLPHGAESRCEVAGYMIPKDTQVLVNAWAIGRDPTIWDEPSEFKPERFVESELEYRGQNFELIPSGAGRRICPGLPLAHRMVHVVIASLLHSFNWSLPDGITADNMDMTEKFGITLQRGSPLIAVPSPRLPAHLFNDQYH

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
1034	20			80	0.995493
1122		14		85	0.993533
1162	85		14		0.993533
1170	71		28		1.0
1196			90	10	0.90298
1208	90		10		0.90298
1222	88			11	0.923564
1271		88	11		0.923564
1276			77	22	0.999999
1302	80			20	0.999996
1361	10		90		0.90298
1405	77		22		0.999999
1474	16	83			0.994512
1475		83		16	0.994512
1476	83		16		0.994512
1477		83	16		0.994512
1478			16	83	0.994512
1537	70		30		1.0
1582		80		20	0.999996
1637	18			9	0.999994
1642		27	72		1.0
1650	81		18		0.999994
1658		77		22	0.999999
1660		11		88	0.923564
1663	77	22			0.999999
1671	22	77			0.999999
1684			14	85	0.993533
1698			85	14	0.993533
1706	85		14		0.993533
1720	14			85	0.993533
1721		85		14	0.993533
1728	14			85	0.993533
1729		85		14	0.993533
1730	14	85			0.993533
1738			85	14	0.993533
1754		16		83	0.994512

SSRs (tot: 2)

Start position	End position	Sequence	Length
1736	1751	ATGT ATGT ATGT ATGT	16
1766	1781	ATAAT ATAAT ATAAT A	16

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain