SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene8330

Length: 2162 nt

UniGene Fasta

>sp_v3.0_unigene8330
TTTTACTTGTCCCGGTGTTCCCGTGACGGGAAAACTGGAAATTGCTGTTGTTTTTCAGTTTATTTTGACGTAATGTGAGCCCCTCACCGTCGTGGAGTCGCCCCTTATACCTCTAGATTTGCTCCATTAATTCGTGCTTCAGAGTTGTTAGAAGAAGAGGCTGCCTGGTGGTGCTCATGGCGGCGCCGAGATCGCTTCACAAGGCCGGTGATTGCTGATTTTTTGGAGCGCGGCTGTTAAAGGCAAGTACCCTTCGCTTATGGCGATTGTGAAGAGTGCTGGTGGGCAGCCGTTGATGTGTTCGATGCCCAAGGGCTCCTGCAGCAATAATACAGGGTCCTTGACGAGCAGCAAGGATACAATATTGGGTTCTGAGCAGACCGGTAACTTGCATGATAGGTATCAGCTGGGGGATGAATTGGGGTTGGGGCAATTTGGTAAGATCAGGTATTGCACAGACAAAGTGACTGGGGAGGTCCTGGCGTGTAAATCCATTGCAAAGGACAGATTGGTGACGTCTGAGGATGTTCGGAGTGTGAAATTGGAGATCGAGATCATGACTAGCCTGTCTGGGCACCCTAACGTTGTGGCCCTGAAGGCAGTCTTTGAGGAGGAGGAATATGTGCATTTGGTTATGGAGCTATGTGCAGGTGGGGAGCTATTTGACCAGTTGCAGAAACAGGGGAGATATTCTGAGCCCGATGCCGCAAGATTGTTCAAGCATCTCATGGAAGTGGTGAAATATTGTCATGATAATGGGGTCGTGCACAGAGATCTGAAGCCAGAAAATATTCTGCTTGCAACAAAGTCTCCTTCGTCGCCGATCAAGCTTGCTGACTTTGGATTGGCCACCTACTTCACACCAGGGCAGAAACTCAATGGAACTGTAGGCAGCCCCTTCTATATAGCTCCAGAAGTTTTGTCTGGAGGATACAATCAAGCAGCAGATGTCTGGAGTGCTGGTGTAATTCTTTACATTCTTCTCAGTGGGATTCCTCCCTTTTGGGGTAAGACAAAATCGAAGATTTTTGATGCAGTCAGAGAAGCCAAGCTGCAATTTCCAAAGGACAATTGGGGCAGTATATCTTCTTCTGCCAAGGATTTGATCTCCAGAATGCTATGCACAGATCCAAAGAAGAGGCTTACCTCTGTGGAAGTGTTAGAGCATCCTTGGATCACTGGTCAAGTAGAGTTATCAAGGCATAGCTCAAATGGGGTTAAAGGGAAACAGGGCATCACCTGTAACTCTTTGAGTTCAGGGGAACCGAAGACAATGGCCTCATTCAGCTCAGCATTTGATTACAACAACAAAACAGATATTTTGTCATCTTTCTCTGGATTTATAGTGACCGATAATGGATGCCAAGTGTTGGGAAGCTTTGCCTTTAATCCCAAAACAAATGGGTCAGAATATTCATCAACCATCCAAAACACATTGAGTTTTGCCTTTGCAAATATTGATGGCACATTTAACTCGGAACATTCTGTATCTTTCTCTGACAATAAAATGCAGCATCAACTTTCTCAAAGAGGGTCAAGTTTTGGACATCTCTTTGCAGCAGCAGTTTCTAACAGTATAAAGAGTGTATCTCAGTATGTGATGTCAGTTGAAGAGGGTGGAGGTCAGACCCAGAAGGCAGAAGCTTCTAATACCAGATACCTTATTACCCACAGAGAGAATCACACAATAGGCTTGGGGGAGTTTGAACAGTTGGATCTCAAGGTTTCAGAATCCATTATACGATGGGCTTCGTGTACTCGGTTATCTGGGTGCACCTCATTTCAATCTCCATTAGTGTGTTGACAGCTTCTGCTAACATCATCAGTGTACCATTGGAAGCCAAAAGCATAGCTTCAAAAACCTAATGATTATCTTTTCTTTTGAAGGGCTGTTTGATGGAGCTTTAACCAGACTTTTGGCAGTCTGTTACTCCTGAGAATTAAATGCATGTGATATATTGATGAACTGATTTGTATTTTTATGGGTTGTATACTACACAGTGGTATAGTAAAGTTCAATGGGAATCTCATAGAGATCCCTTCTTTTGATAGTGTAAAAGTGCTTATTCTTATGAGTGGATAATAAATAAAAGTATCAAGCATGTCTATATTTTTATGTATTATATAATTCAAGAATGAGATGTCAGATCTCGATTGTCTAC

Ace file of the UniGene sp_v3.0_unigene8330

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	CDPK-like protein n=1 Tax=Solanum tuberosum RepID=Q8LPM2_SOLTU	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	52%
Sma3	Calcium-dependent protein kinase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Non-specific serine/threonine protein kinase.	EC:2.7.11.1	-	0.0	-
Sma3	Calcium/calmodulin-dependent protein kinase.	EC:2.7.11.17	-	3.716e-25	-

Source	Gene names
Sma3	6J23.10; AK1; At1g12580; At1g18890; At1g35670; At1g50700; At1g74740; At1g76040; At2g17290; At2g35890; At2g38910; At2g41860; At3g10660; At3g20410; At3g57530; At4g04720; At4g09570; At4g23650; At4g35310; At4g38230; At5g04870; At5g12180; At5g12480; At5g19360;

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	peroxisomal membrane	GO:0005778	Cellular Component	-
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	protein kinase activity	GO:0004672	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	calcium-dependent protein kinase C activity	GO:0004698	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	signal transduction	GO:0007165	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	abscisic acid mediated signaling pathway	GO:0009738	Biological Process	-
Sma3	positive regulation of abscisic acid mediated signaling pathway	GO:0009789	Biological Process	-
Sma3	regulation of stomatal movement	GO:0010119	Biological Process	-
Sma3	regulation of anion channel activity	GO:0010359	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Histidine phosphatase superfamily, clade-2	IPR000560	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Parvalbumin	IPR008080	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-
Sma3	ATPase, P-type phosphorylation site	IPR018303	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G12580.1	PEPKR1 phosphoenolpyruvate carboxylase-related kinase 1 chr1:4283635-4285675 FORWARD LENGTH=522	51%
RefSeq	Arabidopsis thaliana	NP_172719.1	phosphoenolpyruvate carboxylase-related kinase 1 [Arabidopsis thaliana]	51%
RefSeq	Populus trichocarpa	XP_002318248.1	predicted protein [Populus trichocarpa]	52%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LP72

Fln msg: your sequence is longer than subject: 515 - 283

Fln protein: MAIVKSAGGQPLMCSMPKGSCSNNTGSLTSSKDTILGSEQTGNLHDRYQLGDELGLGQFGKIRYCTDKVTGEVLACKSIAKDRLVTSEDVRSVKLEIEIMTSLSGHPNVVALKAVFEEEEYVHLVMELCAGGELFDQLQKQGRYSEPDAARLFKHLMEVVKYCHDNGVVHRDLKPENILLATKSPSSPIKLADFGLATYFTPGQKLNGTVGSPFYIAPEVLSGGYNQAADVWSAGVILYILLSGIPPFWGKTKSKIFDAVREAKLQFPKDNWGSISSSAKDLISRMLCTDPKKRLTSVEVLEHPWITGQVELSRHSSNGVKGKQGITCNSLSSGEPKTMASFSSAFDYNNKTDILSSFSGFIVTDNGCQVLGSFAFNPKTNGSEYSSTIQNTLSFAFANIDGTFNSEHSVSFSDNKMQHQLSQRGSSFGHLFAAAVSNSIKSVSQYVMSVEEGGGQTQKAEASNTRYLITHRENHTIGLGEFEQLDLKVSESIIRWASCTRLSGCTSFQSPLVC*

Protein Length: 516

Fln nts: TTTTACTTGTCCCGGTGTTCCCGTGACGGGAAAACTGGAAATTGCTGTTGTTTTTCAGTTTATTTTGACGTAATGTGAGCCCCTCACCGTCGTGGAGTCGCCCCTTATACCTCTAGATTTGCTCCATTAATTCGTGCTTCAGAGTTGTTAGAAGAAGAGGCTGCCTGGTGGTGCTCATGGCGGCGCCGAGATCGCTTCACAAGGCCGGTGATTGCTGATTTTTTGGAGCGCGGCTGTTAAAGGCAAGTACCCTTCGCTT_-_ATGGCGATTGTGAAGAGTGCTGGTGGGCAGCCGTTGATGTGTTCGATGCCCAAGGGCTCCTGCAGCAATAATACAGGGTCCTTGACGAGCAGCAAGGATACAATATTGGGTTCTGAGCAGACCGGTAACTTGCATGATAGGTATCAGCTGGGGGATGAATTGGGGTTGGGGCAATTTGGTAAGATCAGGTATTGCACAGACAAAGTGACTGGGGAGGTCCTGGCGTGTAAATCCATTGCAAAGGACAGATTGGTGACGTCTGAGGATGTTCGGAGTGTGAAATTGGAGATCGAGATCATGACTAGCCTGTCTGGGCACCCTAACGTTGTGGCCCTGAAGGCAGTCTTTGAGGAGGAGGAATATGTGCATTTGGTTATGGAGCTATGTGCAGGTGGGGAGCTATTTGACCAGTTGCAGAAACAGGGGAGATATTCTGAGCCCGATGCCGCAAGATTGTTCAAGCATCTCATGGAAGTGGTGAAATATTGTCATGATAATGGGGTCGTGCACAGAGATCTGAAGCCAGAAAATATTCTGCTTGCAACAAAGTCTCCTTCGTCGCCGATCAAGCTTGCTGACTTTGGATTGGCCACCTACTTCACACCAGGGCAGAAACTCAATGGAACTGTAGGCAGCCCCTTCTATATAGCTCCAGAAGTTTTGTCTGGAGGATACAATCAAGCAGCAGATGTCTGGAGTGCTGGTGTAATTCTTTACATTCTTCTCAGTGGGATTCCTCCCTTTTGGGGTAAGACAAAATCGAAGATTTTTGATGCAGTCAGAGAAGCCAAGCTGCAATTTCCAAAGGACAATTGGGGCAGTATATCTTCTTCTGCCAAGGATTTGATCTCCAGAATGCTATGCACAGATCCAAAGAAGAGGCTTACCTCTGTGGAAGTGTTAGAGCATCCTTGGATCACTGGTCAAGTAGAGTTATCAAGGCATAGCTCAAATGGGGTTAAAGGGAAACAGGGCATCACCTGTAACTCTTTGAGTTCAGGGGAACCGAAGACAATGGCCTCATTCAGCTCAGCATTTGATTACAACAACAAAACAGATATTTTGTCATCTTTCTCTGGATTTATAGTGACCGATAATGGATGCCAAGTGTTGGGAAGCTTTGCCTTTAATCCCAAAACAAATGGGTCAGAATATTCATCAACCATCCAAAACACATTGAGTTTTGCCTTTGCAAATATTGATGGCACATTTAACTCGGAACATTCTGTATCTTTCTCTGACAATAAAATGCAGCATCAACTTTCTCAAAGAGGGTCAAGTTTTGGACATCTCTTTGCAGCAGCAGTTTCTAACAGTATAAAGAGTGTATCTCAGTATGTGATGTCAGTTGAAGAGGGTGGAGGTCAGACCCAGAAGGCAGAAGCTTCTAATACCAGATACCTTATTACCCACAGAGAGAATCACACAATAGGCTTGGGGGAGTTTGAACAGTTGGATCTCAAGGTTTCAGAATCCATTATACGATGGGCTTCGTGTACTCGGTTATCTGGGTGCACCTCATTTCAATCTCCATTAGTGTGTTGA___CAGCTTCTGCTAACATCATCAGTGTACCATTGGAAGCCAAAAGCATAGCTTCAAAAACCTAATGATTATCTTTTCTTTTGAAGGGCTGTTTGATGGAGCTTTAACCAGACTTTTGGCAGTCTGTTACTCCTGAGAATTAAATGCATGTGATATATTGATGAACTGATTTGTATTTTTATGGGTTGTATACTACACAGTGGTATAGTAAAGTTCAATGGGAATCTCATAGAGATCCCTTCTTTTGATAGTGTAAAAGTGCTTATTCTTATGAGTGGATAATAAATAAAAGTATCAAGCATGTCTATATTTTTATGTATTATATAATTCAAGAATGAGATGTCAGATCTCGATTGTCTAC

Fln Alignment:

Contig8330___TGNLHDRYQLGDE-LGLGQFGKIRYCTDKVTGEVLACKSIAKDRLVTSEDVRSVKLEIEIMTSLSGHPNVVALKAVFEEEEYVHLVMELCAGGELFDQLQKQGR---YSEPDAARLFKHLMEVVKYCHDNGVVHRDLKPENILLATKSPS-SPIKLADFGLATYFTPGQKL-NGTVGSPFYIAPEVLSG-GYNQAADVWSAGVILYILLSGIPPFWGKTKSKIFDAVREAKLQFPKDNWGSISSSAKDLISRMLCTDPKKRLTSVEVLEHPWIT
B8LP72______________TDALLQDYQLQEEVIGRGRFGVIRRCFNKRSGQQAACKTIRKADLHDSIDRECLNSEIKIMQYVSGHPTVVALHDVYEDDDFVHLVMEMCSGGDLFDRIVSKKSTVPFSEEEAATILQKLMEAISYCHSMGIAHRDIKPDNILFANNSNFLTEIKLADFGQASSFSPGQGLMQGIVGTPYYVAPEVLGGKDYSEKVDVWSAGVIVYIMLSGVAPFLGDTPQEIFEAVLCGRLRFPSDPWLSISNSAKDLIRRMVCRDVTKRLSAHQVLEHPWIT

SNPs (tot: 40)

Position	A %	C %	G %	T %	Probability
173	12			87	0.992554
231	11			55	1.0
259		44		55	1.0
276			88	11	0.923564
339		25		75	1.0
490		75	25		0.996473
499		25		75	0.996473
595		75		25	0.996473
661	85			14	0.993533
673	85			14	0.993533
757	10			90	0.90298
758		10		90	0.90298
759	10		90		0.90298
761		90		10	0.90298
767	90		10		0.90298
768	90		10		0.90298
769			10	90	0.90298
1060		90		10	0.90298
1234	87		12		0.992554
1257	90		10		0.90298
1483		25		75	1.0
1647	88	11			0.923564
1649			88	11	0.923564
1651	88		11		0.923564
1652			88	11	0.923564
1653	88		11		0.923564
1655		11	88		0.923564
1660			14	85	0.993533
1661	85		14		0.993533
1662	85	14			0.993533
1664	85		14		0.993533
1665		85		14	0.993533
1669	85	14			0.993533
1673	14	85			0.993533
1696		16		83	0.994512
1711	16		83		0.994512
1777	57		42		1.0
1983	22			77	0.999999
2105	11		88		0.999952
2132			20	80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain