SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene8285

Length: 1939 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene8285

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	cytochrome P450 enzyme [Pisum sativum]	-	-	59%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	87%
Sma3	Putative uncharacterized protein	-	-	-

Source	GOs	Term	Type	Identity
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3		IPR017973	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G30180.1	CYP85A2, BR6OX2 brassinosteroid-6-oxidase 2 chr3:11810867-11813509 FORWARD LENGTH=465	55%
RefSeq	Arabidopsis thaliana	NP_566852.1	brassinosteroid-6-oxidase 2 [Arabidopsis thaliana]	55%
RefSeq	Populus trichocarpa	XP_002330918.1	cytochrome P450 [Populus trichocarpa]	58%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9P221

Fln msg: your sequence is longer than subject: 471 - 432

Fln protein: MDILSTMALGFIFLCSIIALLRWNEVRYGKKGLPPGTMGWPLFGETPDFLRYGQQFIKNRKARYGDLFKTHILGCPTVISTDPAVNRYILLNEGRGLIPGYPQSMLDILGKWNIAAVQSDLHKTMRGAMLALINPSMIKGHLLSDINEFMDIHLQHWNDRVINLQDKTKEMALLLSLQQVMSIKSGPKAEAFVSEFCKLVEGTISMPINLPGTSYKRGFQARENILRMLREVLKERRASMDTYNDMIAMLLNDKEGEADGTSANCKLTDEQIFDLLISIINAGYETVSTTTMMAVKYLHDSPEALRELREEHLAIQRRKNPGETVNWDDYKSMKFTQSVIYETLRLATVVNGVLRKTTQDMEMRGFMIPKGWKIYVYMTEAHHDNFLYPDFSAFNPWRWQEKNGDSVMYFTAFGGGSRLCPGKELGLVQISMFLHHFVTRYRWEEVGGDEILKFPRVVAPKGLRIKVSEY*

Protein Length: 472

Fln nts: TATTGGGGGTGCAGCTCTGGGCTACATGGAAAGTTTCGAAGTGGGGTCGTTCTTTTTCCAACGAACAATGATTAACAGGGCTGCTTTATATCACATATCTCAAATCGCCTGAGGTTGCACAGGATCCTATTAGAGAGGCAAGACTGAGGCCTAATTGTGGTATTTGAGGGGATTTGAAGGCCTGAAAATTCAAAAGGGCCAACGTGGGTACGAGGCCAGAG_-_ATGGATATTTTAAGCACAATGGCTTTGGGTTTCATTTTTCTCTGCAGTATAATCGCTTTGCTGCGCTGGAATGAAGTTAGATACGGCAAGAAAGGCCTTCCTCCAGGCACCATGGGATGGCCTCTCTTTGGAGAAACTCCTGATTTTCTCAGATATGGTCAACAATTTATCAAAAACAGAAAGGCCAGATATGGAGATTTGTTCAAGACTCACATTCTAGGATGCCCGACGGTGATATCGACGGATCCAGCTGTCAACAGATATATCTTATTGAATGAAGGCCGAGGACTAATTCCTGGATACCCGCAGTCTATGCTTGACATATTGGGAAAATGGAACATTGCTGCTGTTCAAAGCGATCTTCATAAAACCATGAGAGGAGCCATGCTCGCATTGATCAATCCCTCCATGATCAAAGGCCACCTGCTTTCTGATATCAACGAGTTCATGGACATACATCTGCAACATTGGAATGACCGCGTAATCAATCTGCAGGATAAAACTAAAGAGATGGCTTTGCTTTTGTCTTTACAGCAGGTAATGAGTATCAAGTCTGGTCCGAAGGCAGAGGCATTTGTGTCAGAATTCTGTAAACTCGTGGAAGGAACCATTTCCATGCCTATAAATCTTCCTGGTACCAGCTACAAACGTGGTTTCCAGGCAAGGGAAAATATCCTGAGAATGTTGAGAGAAGTGCTCAAGGAAAGGAGGGCTTCCATGGACACTTATAATGATATGATTGCAATGCTTCTTAATGACAAAGAAGGAGAAGCAGATGGCACATCTGCTAATTGCAAGCTCACAGATGAACAGATCTTCGATTTACTAATATCTATTATCAATGCTGGCTACGAAACAGTTTCCACTACAACCATGATGGCTGTCAAGTATCTCCATGACAGTCCCGAGGCTCTCCGAGAGCTTAGAGAAGAACACCTAGCAATTCAGAGGAGAAAAAATCCAGGCGAAACTGTCAATTGGGATGACTACAAGTCTATGAAGTTTACTCAGAGTGTGATATATGAAACGCTGAGACTTGCAACTGTTGTAAATGGAGTTTTGAGAAAAACAACTCAAGATATGGAAATGAGAGGTTTCATGATTCCCAAAGGATGGAAAATTTATGTTTACATGACAGAAGCCCATCACGATAATTTTCTCTATCCAGACTTCTCCGCATTTAACCCATGGCGATGGCAGGAAAAGAACGGTGACTCCGTCATGTATTTCACGGCTTTCGGAGGAGGAAGCAGGCTGTGCCCAGGCAAGGAATTGGGTTTAGTTCAGATATCCATGTTTCTCCACCATTTTGTCACTCGCTACAGATGGGAGGAGGTCGGGGGAGATGAAATTTTGAAATTTCCCAGGGTTGTGGCTCCTAAAGGACTTCGCATCAAAGTATCTGAGTACTGA___ATCAATGAAAATGCCACAGTGCATCAACCATAGGGCTGTTTCTCTCAAATATACTAAATGTCATGTATAGAATATCTAATTTTATGTTTAAGCTCCATGTATATCTTCTGTTGAAGAAAGAGTAACTATCATCAAATACACATGATTACAGAGATCAATTTTGAAGAGATTATTCTACCTTGTGATGTGAAACAATATAAAACTGTTGATAAAAATGAGATGTTTTTTTCCCTATGATTACATAGATCAATTTTGAAGAGATTGTTCTACCTTGTGATGTGAAACAATTTAAAACTGTTGATAAA

Fln Alignment:

Contig8285___MGWPLFGETPDFLRYGQQFIKNRKARYGDLFKTHILGCPTVISTDPAVNRYILLNEGRGLIPGYPQSMLDILGKWNIAAVQSDLHKTMRGAMLALINPSMIKGHLLSDINEFMDIHLQHWNDRVINLQDKTKEMALLLSLQQVMSIKSGPKAEAFVSEFCKLVEGTISMPINLPGTSYKRGFQARENILRMLREVLKERRASMDTYNDMIAMLLNDKEGEADGTSANCKLTDEQIFDLLISIINAGYETVSTTTMMAVKYLHDSPXXXXXXXXXXXXIQRRKNPGETVNWDDYKSMKFTQSVIYETLRLATVVNGVLRKTTQDMEMRGFMIPKGWKIYVYMTEAHHDNFLYPDFSAFNPWRWQEKNGDSVMYFTAFGGGSRLCPGKELGLVQISMFLHHFVTRYRWEEVGGDEILKFPRVVAPKGLRIKVSEY
A9P221______________MGWPLFGETSDFLKSGQKFIKIRRARYGELFRSHILGCPTVISTDPALNRYILLNEGRGLIPGYPQSMLDILGKWNIAAVQNSLHKTMRGAMLALINPSMIKDHLLSDINNFMDIHFQHWNDRVINLQDKTKEMALLLSLKQVMSMNSGPKAEAFMLEFYKLVEGTISMPINLPGTSYRRGFQARENVLRMLREVLRERRASTDTHNDMIAMLLNDKE-EEDGASVNSKLTDEQILDLLISIINAGYETVSTTTMMAVKYLHDNPEALRQLREEHLAIKRRKNPGETVNWDDYKSMKFTRSVIYETLRIATIVNGVLRKTTQDMEMKGFLIPKGWRIYVYMAETNQDNFLYPDFSTFNPWRWQEKNGDSLLYFMAFGGGSRLCPGKELGLVEISMFLHYFVTRYRWEEVGGDEILSFPRVVAPKGLRIKVSEY

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
115			25	75	0.996473
116			25	75	0.996473
214			42	57	1.0
240	85		14		0.993533
384		90	10		0.90298
385	88			11	0.923564
460		85		14	0.993533
720	73		26		1.0
855	16		83		0.994512
993			66	33	0.997452
1011	66			33	0.997452
1014			33	66	0.997452
1277	83		16		0.994512
1292	83		16		0.994512
1410		11		88	0.999937
1439		88		11	0.999937
1562			80	20	0.999996
1590		12	87		0.992554
1617	83			16	0.994512
1663		53	46		1.0
1691	91	8			0.991935
1731	66	33			1.0
1744		20	80		0.997017
1775		66		33	0.99834

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain