SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene8102

Length: 1757 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene8102

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	cytochrome P450, family 94, subfamily B, polypeptide 1 [Arabidopsis thaliana] dbj\|BAB08810.1\| cytochrome P450-like protein [Arabidopsis thaliana] gb\|ABA61323.1\| cytochrome P450 CYP94B1 [Arabidopsis thaliana] gb\|AED97750.1\| cytochrome P450, family 94, subf	-	-	0.0	52%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	54%
Sma3	Cytochrome P450	-	-	8.616e-26	-

Source	ECs	Term	Type	e value	Identity
Sma3	Unspecific monooxygenase.	EC:1.14.14.1	-	4.44e-33	-

Source	KEGGs	Term	e value	Identity
Sma3	Fatty acid metabolism	00071	4.44e-33	%
Sma3	Steroid hormone biosynthesis	00140	4.44e-33	%
Sma3	Caffeine metabolism	00232	4.44e-33	%
Sma3	Tryptophan metabolism	00380	4.44e-33	%
Sma3	Arachidonic acid metabolism	00590	4.44e-33	%
Sma3	Linoleic acid metabolism	00591	4.44e-33	%
Sma3	Aminobenzoate degradation	00627	4.44e-33	%
Sma3	Retinol metabolism	00830	4.44e-33	%
Sma3	Metabolism of xenobiotics by cytochrome P450	00980	4.44e-33	%
Sma3	Drug metabolism - cytochrome P450	00982	4.44e-33	%
Sma3	Drug metabolism - other enzymes	00983	4.44e-33	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	4.44e-33	%
Sma3	Metabolic pathways	01100	4.44e-33	%

Source	Gene names
Sma3	AT4g39490; A_IG005I10.21; At1g01600; At1g01600/F22L4_10; At1g24540; At1g34540; At2g23180; At2g23180/T20D16.19; At2g27690; At2g27690/F15K20.21; At2g44890; At2g45510; At2g45970; At3g01900; At3g26125; At3g48520; At3g56630; At4g00360; At4g39490; At5g08250; At

Source	GOs	Term	Type	Identity
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	microsome	GO:0005792	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3		GO:0008393	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	fatty acid metabolic process	GO:0006631	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	suberin biosynthetic process	GO:0010345	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	ATPase, F1 complex, OSCP/delta subunit	IPR000711	-	-
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Legume lectin domain	IPR001220	-	-
Sma3	Phosphoglycerate/bisphosphoglycerate mutase, active site	IPR001345	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Cytochrome P450, E-class, group IV	IPR002403	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Retrotransposon gag protein	IPR005162	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	Histidine phosphatase superfamily, clade-1	IPR013078	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G63450.1	CYP94B1 cytochrome P450, family 94, subfamily B, polypeptide 1 chr5:25408987-25410519 REVERSE LENGTH=510	54%
RefSeq	Arabidopsis thaliana	NP_201150.1	cytochrome P450, family 94, subfamily B, polypeptide 1 [Arabidopsis thaliana]	54%
RefSeq	Populus trichocarpa	XP_002318721.1	predicted protein [Populus trichocarpa]	50%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LML7

Fln msg: Separated hits, possible frame ERROR between 736 and 777,

Fln protein: MLAESPTQTILVERLGAVRTVVTANPENIKYILKTQFDNYPKGKPFTAILHDLLGKGIFNSDGNAWKLQRKVASYEFNTRSLREFVLRVVEEVTDRLLPILDEASQTGRGLDFQDILQRFAFDNICKVAFGIDPASLDASLSVSELAQAFDVATELSARRSTAPISLTWKIKRMLNIGSEKRLRCAVKAVHEFAMDVIRQRRREMSSTGPCKQDLLSRFMALxxxxxxxxxxxxxxxSEEFLRDTIISFLLAGRDSTSAALTWFFWLLSSHPHVEEAIRREIACLMATRPEPKCHNPFVFSYKELQSMHYLHASICESMRLYPPVPWDSKHALHNDVLPDGTFVPKGTRVTYHSYAMGRINAIWGSDCLQFKPERWINEEGHFVPKCPFKFPVFHGGVRVCLGKDMAFLQMKYIAASVIPMFRIRPIHRNADPKYATSLTARMVGGLPVVVERVPQVLHDVL*

Protein Length: 464

Fln nts: ATCCGGTCGTGGGTTCTTTATTTGCGTTTCCAAGAATGGAGGCCGCCTGCTTCAATGGTACGCAGAA_-_ATGCTCGCAGAATCCCCCACGCAGACCATTCTGGTCGAGCGACTGGGAGCGGTTCGGACGGTTGTGACGGCGAATCCTGAGAATATTAAGTATATACTGAAGACCCAATTCGACAATTATCCAAAGGGGAAGCCCTTTACGGCCATTCTGCACGATCTGTTGGGGAAGGGGATCTTTAATTCCGACGGGAATGCCTGGAAATTGCAGCGCAAGGTTGCCAGCTACGAGTTTAACACTCGCTCTCTGCGGGAATTTGTTCTGCGAGTCGTCGAAGAGGTCACGGATCGACTCCTGCCGATTCTTGACGAGGCCAGCCAGACGGGCAGAGGATTGGATTTTCAGGACATTCTGCAGCGTTTCGCGTTTGATAATATATGCAAAGTTGCGTTCGGGATCGATCCGGCTAGCCTTGATGCGAGCCTTTCTGTTTCGGAGCTCGCGCAGGCTTTCGATGTGGCGACCGAGCTCAGCGCTCGGCGCTCCACTGCGCCGATCTCGTTGACGTGGAAGATAAAGAGGATGCTCAACATTGGGTCGGAAAAACGCCTCCGCTGCGCAGTAAAGGCCGTGCACGAGTTCGCCATGGATGTCATTCGGCAGCGGAGGCGGGAAATGAGCAGCACCGGTCCCTGCAAACAGGACCTGCTGTCAAGATTCATGGCGCTGGCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCTCCGAAGAATTTCTCCGCGATACCATCATAAGTTTCCTCTTGGCCGGCAGAGACAGCACCTCGGCTGCACTAACCTGGTTCTTCTGGCTGCTCTCCTCACATCCCCATGTCGAGGAAGCCATACGCAGAGAAATCGCGTGTCTCATGGCCACCAGGCCCGAACCCAAATGCCATAATCCCTTCGTATTCTCATACAAAGAGCTGCAGAGCATGCATTACCTGCACGCCTCAATCTGCGAATCCATGAGACTTTACCCGCCGGTCCCTTGGGACTCGAAGCACGCCCTCCACAACGACGTCCTGCCCGACGGGACTTTTGTTCCCAAAGGCACTCGGGTAACTTACCACTCCTACGCCATGGGTCGTATAAATGCCATCTGGGGATCCGACTGTCTGCAATTCAAGCCGGAGAGATGGATCAACGAGGAAGGCCATTTTGTCCCCAAGTGTCCCTTCAAGTTCCCAGTCTTCCATGGCGGAGTTCGTGTGTGTCTGGGAAAAGATATGGCGTTCCTTCAGATGAAATACATCGCTGCCTCTGTGATTCCCATGTTCAGAATACGTCCAATCCACCGTAATGCAGATCCAAAGTACGCTACCAGCTTGACTGCGCGCATGGTGGGCGGTCTTCCTGTTGTCGTTGAGAGGGTACCCCAAGTCCTCCACGATGTATTGTGA___CTTCCGCCTTCTATCAATCAATTTTCTTTTAACTTCCTGCCATAGGCGGCCCCACCGGAAGGGGCCACACACTCAGTCGCCTTAAAGGTAGGAGCCCAGGGGAATTGAACCCACGCCTTATGTCTAGTGATAAAGGGTCTAACACTTGGCATGGATTCGACAGTCTGGCTATCCATCGAAGCTTTTGTCAATCAATTTTACATGGGCGAACGTTATATAATTTATTGGAATCTTTATGGAGTTTTCATAAATCCTTACTATATTGTTAAACACTAAAGGAGAAGTCTAACTATCAGGGTTTTT

Fln Alignment:

Contig8102___LVERLGAVRTVVTANPENIKYILKTQFDNYPKGKPFTAILHDLLGKGIFNSDGNAWKLQRKVASYEFNTRSXXXXXXXXXXXXT-DRLLPILDEASQTGRGLDFQDILQRFAFDNICKVAFGIDPASLDASLSVSELAQAFDVATELSARRSTAPISLTWKIKRMLNIGSEKRLRCAVKAVHEFAMDVIRQRRREMSSTGPCKQDLLSRFMALAxxxxxxxxxxxxxxSEEFLRDTIISFLLAGRDSTSAALTWFFWLLSSHPHVEEAIRREIACLMATRP----EPKCHNPFVFSYKELQSMHYLHASICESMRLYPPVPWDSKHALHNDVLPDGTFVPKGTRVTYHSYAMGRINAIWGSDCLQFKPERWINEEGHXXXXXXXXXXXXHGGVRVCLGKDMAFLQMKYIAASVIPMFRIRPIHRNADPKYATSLTARMVGGLPVVVE
B8LML7______________MIQRPGGVRGIITANPSNVEHILRANFQNYPKGERFSFLLHDFLGRGILNTDGELWKMQRKAASYEFNTKSLRNFVVEAVQWEIKNRLMAVLKNACERGEVIELQDVLMRFAFDNICKVAFGVDPGCLHPSLPRSQFAEAFNDATGISAGRFFSSVPFLWRIKKMLDIGSEKRLREAISVVDEFVMDIIRSRRSEISE-GHLREDLLSRFMAAAxxxxxxxxxxxxxxSELFVRDMMVSFMMAGRDTTSTALTWFFWLISSRPHIQEAIRREILLVKGNRNSITVEPE-DQWVVFDFDELKGMQYLHAALCESMRLWPPVPVDTKVAATDDVLPDGTVVPRGWFVSYSIYSMGRMESIWGGDFMDFRPERWL-KDGEFVGENPYKFTVFQAGPRICLGKEMAFIQMKSIVASVIHRFSLE-VDSEYVPKYAMSITLRMKTGLPVNVK

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
533	87		12		0.992554
595	88		11		0.923564
608	87		12		0.992554
612	87			12	0.992554
613		87	12		0.992554
617	12			87	0.992554
621		87	12		0.992554
623		12	87		0.992554
625		12	87		0.992554
626		87		12	0.992554
737	16	83			0.994512
739	83	16			0.994512
1404	9		90		1.0
1447	7			92	0.999532

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain