SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene7950

Length: 1112 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene7950 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	ubiquitin conjugating enzyme-like [Oryza sativa Japonica Group]	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	48%
Sma3	Ubiquitin carrier protein	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Ligases, Forming carbon-nitrogen bonds, Acid--D-amino-acid ligases (peptide synthases).	EC:6.3.2.-	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Tryptophan metabolism	00380		0.0	%
Sma3	Biosynthesis of siderophore group nonribosomal peptides	01053		0.0	%
Sma3	Ubiquitin--protein ligase.	EC:6.3.2.19	-	1.025e-18	-

Source	Gene names
Sma3	At1g16890; At1g36340; At1g64230; At1g78870; At2g16740; At3g08690; At3g24515; At4g27960; At5g41700; At5g53300; At5g56150; B0811B10.8; B1043F11.37; CHLREDRAFT_129070; CHLREDRAFT_152525; CHLREDRAFT_77514; F17F16.19; F17O14.16; F22C12.2; F6I1.11; F7F23.6; F9K

Source	GOs	Term	Type	Identity
Sma3	intracellular	GO:0005622	Cellular Component	-
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	UBC13-MMS2 complex	GO:0031372	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	endonuclease activity	GO:0004519	Molecular Function	-
Sma3	ubiquitin-protein ligase activity	GO:0004842	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	small conjugating protein ligase activity	GO:0019787	Molecular Function	-
Sma3	4 iron, 4 sulfur cluster binding	GO:0051539	Molecular Function	-
Sma3	base-excision repair	GO:0006284	Biological Process	-
Sma3	ubiquitin-dependent protein catabolic process	GO:0006511	Biological Process	-
Sma3	modification-dependent protein catabolic process	GO:0019941	Biological Process	-
Sma3	post-translational protein modification	GO:0043687	Biological Process	-
Sma3	cell redox homeostasis	GO:0045454	Biological Process	-
Sma3	regulation of protein metabolic process	GO:0051246	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	NUDIX hydrolase domain	IPR000086	-	-
Sma3	Helix-hairpin-helix motif	IPR000445	-	-
Sma3	Ubiquitin-conjugating enzyme, E2	IPR000608	-	-
Sma3	HhH-GPD domain	IPR003265	-	-
Sma3	Endonuclease III-like, iron-sulphur cluster loop motif	IPR003651	-	-
Sma3	Endonuclease III, iron-sulphur binding site	IPR004035	-	-
Sma3	Thioredoxin domain	IPR013766	-	-
Sma3	Ubiquitin-conjugating enzyme/RWD-like	IPR016135	-	-
Sma3		IPR017936	-	-
Sma3	Thioredoxin, conserved site	IPR017937	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G24515.1	UBC37 ubiquitin-conjugating enzyme 37 chr3:8934569-8936207 REVERSE LENGTH=409	67%
RefSeq	Arabidopsis thaliana	NP_566751.1	putative ubiquitin-conjugating enzyme E2 37 [Arabidopsis thaliana]	67%
RefSeq	Populus trichocarpa	XP_002333348.1	predicted protein, partial [Populus trichocarpa]	68%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NLA0

Fln msg: your sequence is longer than subject: 260 - 148

Fln protein: MNEHSYSVSQQTQIARLSSRMQRELKRLQTDPPPGICAWPSNDNLLTRLEAQIEGPEGTVYAKGIFKLEIQVPERYPFEPPNVKFVTPIYHPNIDNGGRICLDILNLPPKGAWAPSLNIATVLASIGLLLSEPNPDDGLMGDISAEYKHNRSNFDQKARSCTQKYAMQMDHQLNTFPMETSCAGSALLTGQTSNKMIDPLKQINEIAAISRSGALDASNHKGEVENDHLVNPVKPTQLLSKKLSLAREPCIAAKKRSFL*

Protein Length: 261

Fln nts: ACATGGGGGGCTTTATATATTTGGTGTCCAGGTAAGGCTTCACTGAGGCGCCATGATTTAAACAGCTCGTGAGGCTTCACTGAGGCGCCATGATTTAAACAGCTCGTTTGAAAGGCTTGCAGGCGAACGAAA_-_ATGAATGAACATTCATACTCTGTCTCACAGCAGACACAAATAGCTCGACTGAGCTCGCGTATGCAGAGAGAACTGAAGCGGCTGCAAACTGATCCTCCTCCCGGAATCTGCGCATGGCCTTCCAATGATAATCTCCTCACTCGCCTTGAAGCGCAGATTGAAGGGCCCGAGGGGACTGTTTATGCTAAAGGGATTTTCAAGCTCGAGATCCAAGTACCCGAAAGATATCCTTTTGAGCCTCCAAATGTGAAATTTGTGACCCCAATTTATCATCCCAATATTGACAACGGAGGGCGTATTTGCCTCGATATCCTGAATTTGCCGCCCAAGGGTGCTTGGGCACCATCCCTGAACATTGCAACTGTCTTGGCAAGTATTGGTTTGCTTCTTTCTGAGCCAAATCCTGATGATGGCCTTATGGGTGATATAAGTGCAGAATACAAGCACAATAGATCTAATTTTGATCAAAAAGCAAGATCATGCACCCAGAAGTATGCAATGCAAATGGATCATCAGCTGAACACTTTTCCAATGGAGACATCATGCGCAGGCAGTGCACTACTGACTGGGCAGACATCAAATAAAATGATTGACCCTCTTAAACAAATCAACGAAATTGCTGCAATATCTCGAAGTGGAGCTCTAGATGCATCAAATCACAAGGGGGAAGTGGAAAATGACCATCTTGTGAATCCTGTAAAACCAACACAGTTGCTGAGCAAGAAACTCTCGTTGGCAAGAGAACCGTGTATTGCAGCCAAGAAAAGATCCTTCTTGTGA___GATTGTGACCGATGGCCAAAGAAAGATGTACAAAGAGAATGTAAATTTGCAACTTGCACGGCATATGGATATGAATGCTCAGCAGAAATTCCATGCCACTTCAGTACTTCAAACAAATGATGCTGAAAACAGGCTGCTTGAAAAAGGTGGAACCGGTGATTCATCTAGTCTGATGCAAAACACATCCAAGGCAGATAGGG

Fln Alignment:

Contig7950___SSRMQRELKRLQTDPPPGICAWPSNDNLLTRLEAQIEGPEGTVYAKGIFKLEIQVPERYPFEPPNVKFVTPIYHPNIDNGGRICLDILNLPPKGAWAPSLNIATVLASIGLLLSEPNPDDGLMGDISAEYKHNRSNFDQKARSCTQKYAM
A9NLA0______________SKRIQKELQDLQRDPPTSCSAGPVGEDLF-HWQATIMGPTDSPYAGGVFFVMIHFPPDYPFKPPKVNFQTKVYHPNINSNGSICLDIL----KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHIYKSQRSRYEETARAWTQKYAM

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
222		80		20	0.995493
252		16		83	0.994512
291	25		75		0.996473
298	75			25	0.996473
299			75	25	0.996473
398		66		33	0.997452
545	25		75		0.996473
662		66		33	0.997452
1005		11		88	0.923564
1006	11			88	0.923564
1084		33		66	0.997452
1087		66	33		0.997452
1088	33			66	0.997452
1089			33	66	0.997452
1090		33	66		0.997452
1091	66			33	0.997452
1092		66	33		0.997452
1093	66			33	0.997452
1094		66		33	0.997452
1095		66		33	0.997452
1100	66	33			0.997452
1104	66		33		0.997452
1105	66		33		0.997452
1106	66	33			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain