SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene7259

Length: 2414 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene7259
AAAATGTTTGGGGGCCGCGGCGCAGGCGTTTGACACAGGACGCTCTGTTGGCCTCGCAAATACAATAAAAGACAAGCAGATAGGAAGAAGCTGCAGCGAGCGAGCTGTGAGCCGAGCTAGAGAGAGAAAGCGATCCAGCCAGCCAGGCCGCCAGGATGTCGCAGGTTCGATCTGCTTACCGTTCCTTGGCTCAGCTGCGTGCTTCCCTTCTCGTTCGTAATATAGGGCCCTGGCGTAACTTCAGCGCAAAAGTTCCCCGCCCCTGCATTGTCCACAAACGCGGCAGCGACATTCTTCACGATCCCTGGTTCAACAAGGAAACTGCGTTTTCAGTGACAGAAAGAGACAGGCTTGGACTTCGTGGCCTTCTTCCACCACGCATTATTTCTTTTCAGCAGCAATGTGATCGTTTCATGGTTTCTTTTAGATCCCTGGAGCATAACACTCGAGATGGTTCAGCTGACTTAACAGCTCTGGCAAAATGGCGTATTCTTAACAGATTGCATGACAGAAATGAAACACTATACTACAAGGTTCTTATAGATCACATTGAAGAGTTTGCTCCAATAATCTACACTCCAACTGTAGGATTGGTTTGTCAGAATTATGGTGGGCTGTTCAGGCGTCCTCGTGGAATGTATTTCAGTGCAAGAGATAAAGGAGAAATGATGTCTATGATTTACAACTGGCCTTCTGAGCAGGTGGATATGATCGTGGTAACTGATGGAAGTCGAATCCTAGGTCTTGGAGATCTTGGAGTCCAAGGAGTTGCTATACCTATTGGAAAGCTTGATATGTACGTGGCTGCTGCTGGAATAAATCCTCAAAGAATTCTCCCTGTCATGCTTGATGTTGGTACAAACAACGTGAAACTTCTCAATAATAGCCTTTATTTGGGGCTGCGACAACCTAGACTGGAAGGAGATGAGTATTTATCCGTACTTGATGAATTTATGGAAGCTGTCTTTACACGATGGCCACAAGTTATTGTACAGTTCGAAGATTTCCAAACAAAATGGGCATTTAAACTTCTGCAGCGATACCGTAAGAGTTTTCGCATGTTCAATGATGATGTTCAGGGTACTGCCGGTGTTGCACTTGCTGGGTTATTAGGTGCAGTCAGGGCTCAGGGTCGTCCAATCACTGATTTTGTCAAACAAAAGATTGTTGTTGTTGGTGCTGGAAGTGCTGGGATCGGAGTTCTTAATACAGCAGAACAGGCAATGTCAAGGATGTTAGGCTCCAATTCGTTATCATCGGCAAACTGTCAGTTCTGGCTTCTGGATAAATCTGGTTTGATTACGCATGCTCGTAAGGACCTTGACCCAGAGGCAGCACCTTTTGCAAGAGGATTTGGGCCCGGAGAAATTGAAGGTTTGCATGAGGGCGCCAGTCTTGTTGAAGTGGTCAAAAAGGTAAAGCCTCATGTGCTTCTAGGTTTATCTGGAGTTGGCGGCATCTTCAGTCAAGAGGTGCTGGAGGCAATGAGAGACTCTGAATCTCCAAGACCTGCAATATTTGCTATGTCAAATCCAACAGAAAATGCTGAATGCACAGCAGCAGATGCATTCAAGTATGCTGGTGAGAACATTGTTTTTGCAAGTGGCAGCCCCTTCCAGGATGTTGATCTAGGAAATGGAAAAATAGGCCATTCAAACCAAGGGAATAACATGTACCTTTTCCCTGGGATTGGACTTGGGACACTTCTTTCAGGTGCCCATATTATTTCAGATAGAATGTTACAAGCAGCTGCTGAGTGCCTTGCAGAGTATATGACAGATGCTGAGATCCAAAGAGGACTTGTTTTCCCTTCCATTTCAAGTATCCGTAATATCACGAAGAGAGTTGCAGCAGCAGTCGTGAAAGCAGCAGTTGCAGAAGACCTTGCAGATGGATATCGAGAAACAGGACCTAAGGAGCTCCGTCAGATGTCAATGGAGGAGACAGTGAAATATGTGGGAGATAATATGTGGTTTCCTGAATATTCACCTCTTGTGCTAGAAAAGTAAGATCCTTAAGATGTTTTGCTAATAAAACTCTTAGGAATGTTGGACCCCATTTCAGAATGCCATTTGAAAAGATTCAAGGTGGTAGCTGTTGCCTGGGGCTGTTTTAGGGGTTTTAGGCCATGCTCTGTAATTTCATTGAGTCAAAATTGGATTAACTGGTGTCTTTTATCCTCATAATAGCTACTGCAGTATTTGTCGATATAGCTTCCCTATTTATTGACTCTCCTTGAGGTACTGTCTGCAGTTAAGAAAGCAAGTGCGGGCACTTCCATTTATGTATGTGACACAAAGTAACAGTTTTGATGTAGTACGATTTGGTAGTCAGATTACTTTTCTTCTACTTGTATGTTCACGTTTATATGTAATTGGTTGGAAGAACTAGGTCCAGTTTCTCATTCTCGGTCG

Ace file of the UniGene sp_v3.0_unigene7259 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Malic enzyme (Fragment) n=1 Tax=Vitis vinifera RepID=D7UC26_VITVI	-	-	67%
FL-Next	sp=Malic enzyme; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	71%
Sma3	Malic enzyme	-	-	-

Source	Gene names
Sma3	GSVIVT00026767001;

Source	GOs	Term	Type	Identity
Sma3	malic enzyme activity	GO:0004470	Molecular Function	-
Sma3	metal ion binding	GO:0046872	Molecular Function	-
Sma3	NAD binding	GO:0051287	Molecular Function	-
Sma3	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	GO:0016616	Molecular Function	-
Sma3	malate metabolic process	GO:0006108	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Malic enzyme, conserved site	IPR015884	-	-
Sma3	Malic enzyme, N-terminal	IPR012301	-	-
Sma3	Malic enzyme, NAD-binding	IPR012302	-	-
Sma3	Malic oxidoreductase	IPR001891	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G00570.1	NAD-ME2 NAD-dependent malic enzyme 2 chr4:242817-246522 REVERSE LENGTH=607	72%
RefSeq	Arabidopsis thaliana	NP_191966.2	malate dehydrogenase (decarboxylating) [Arabidopsis thaliana]	72%
RefSeq	Populus trichocarpa	XP_002301340.1	predicted protein [Populus trichocarpa]	75%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LMR7

Fln msg:

Fln protein: MSQVRSAYRSLAQLRASLLVRNIGPWRNFSAKVPRPCIVHKRGSDILHDPWFNKETAFSVTERDRLGLRGLLPPRIISFQQQCDRFMVSFRSLEHNTRDGSADLTALAKWRILNRLHDRNETLYYKVLIDHIEEFAPIIYTPTVGLVCQNYGGLFRRPRGMYFSARDKGEMMSMIYNWPSEQVDMIVVTDGSRILGLGDLGVQGVAIPIGKLDMYVAAAGINPQRILPVMLDVGTNNVKLLNNSLYLGLRQPRLEGDEYLSVLDEFMEAVFTRWPQVIVQFEDFQTKWAFKLLQRYRKSFRMFNDDVQGTAGVALAGLLGAVRAQGRPITDFVKQKIVVVGAGSAGIGVLNTAEQAMSRMLGSNSLSSANCQFWLLDKSGLITHARKDLDPEAAPFARGFGPGEIEGLHEGASLVEVVKKVKPHVLLGLSGVGGIFSQEVLEAMRDSESPRPAIFAMSNPTENAECTAADAFKYAGENIVFASGSPFQDVDLGNGKIGHSNQGNNMYLFPGIGLGTLLSGAHIISDRMLQAAAECLAEYMTDAEIQRGLVFPSISSIRNITKRVAAAVVKAAVAEDLADGYRETGPKELRQMSMEETVKYVGDNMWFPEYSPLVLEK*

Protein Length: 619

Fln nts: AAAATGTTTGGGGGCCGCGGCGCAGGCGTTTGACACAGGACGCTCTGTTGGCCTCGCAAATACAATAAAAGACAAGCAGATAGGAAGAAGCTGCAGCGAGCGAGCTGTGAGCCGAGCTAGAGAGAGAAAGCGATCCAGCCAGCCAGGCCGCCAGG_-_ATGTCGCAGGTTCGATCTGCTTACCGTTCCTTGGCTCAGCTGCGTGCTTCCCTTCTCGTTCGTAATATAGGGCCCTGGCGTAACTTCAGCGCAAAAGTTCCCCGCCCCTGCATTGTCCACAAACGCGGCAGCGACATTCTTCACGATCCCTGGTTCAACAAGGAAACTGCGTTTTCAGTGACAGAAAGAGACAGGCTTGGACTTCGTGGCCTTCTTCCACCACGCATTATTTCTTTTCAGCAGCAATGTGATCGTTTCATGGTTTCTTTTAGATCCCTGGAGCATAACACTCGAGATGGTTCAGCTGACTTAACAGCTCTGGCAAAATGGCGTATTCTTAACAGATTGCATGACAGAAATGAAACACTATACTACAAGGTTCTTATAGATCACATTGAAGAGTTTGCTCCAATAATCTACACTCCAACTGTAGGATTGGTTTGTCAGAATTATGGTGGGCTGTTCAGGCGTCCTCGTGGAATGTATTTCAGTGCAAGAGATAAAGGAGAAATGATGTCTATGATTTACAACTGGCCTTCTGAGCAGGTGGATATGATCGTGGTAACTGATGGAAGTCGAATCCTAGGTCTTGGAGATCTTGGAGTCCAAGGAGTTGCTATACCTATTGGAAAGCTTGATATGTACGTGGCTGCTGCTGGAATAAATCCTCAAAGAATTCTCCCTGTCATGCTTGATGTTGGTACAAACAACGTGAAACTTCTCAATAATAGCCTTTATTTGGGGCTGCGACAACCTAGACTGGAAGGAGATGAGTATTTATCCGTACTTGATGAATTTATGGAAGCTGTCTTTACACGATGGCCACAAGTTATTGTACAGTTCGAAGATTTCCAAACAAAATGGGCATTTAAACTTCTGCAGCGATACCGTAAGAGTTTTCGCATGTTCAATGATGATGTTCAGGGTACTGCCGGTGTTGCACTTGCTGGGTTATTAGGTGCAGTCAGGGCTCAGGGTCGTCCAATCACTGATTTTGTCAAACAAAAGATTGTTGTTGTTGGTGCTGGAAGTGCTGGGATCGGAGTTCTTAATACAGCAGAACAGGCAATGTCAAGGATGTTAGGCTCCAATTCGTTATCATCGGCAAACTGTCAGTTCTGGCTTCTGGATAAATCTGGTTTGATTACGCATGCTCGTAAGGACCTTGACCCAGAGGCAGCACCTTTTGCAAGAGGATTTGGGCCCGGAGAAATTGAAGGTTTGCATGAGGGCGCCAGTCTTGTTGAAGTGGTCAAAAAGGTAAAGCCTCATGTGCTTCTAGGTTTATCTGGAGTTGGCGGCATCTTCAGTCAAGAGGTGCTGGAGGCAATGAGAGACTCTGAATCTCCAAGACCTGCAATATTTGCTATGTCAAATCCAACAGAAAATGCTGAATGCACAGCAGCAGATGCATTCAAGTATGCTGGTGAGAACATTGTTTTTGCAAGTGGCAGCCCCTTCCAGGATGTTGATCTAGGAAATGGAAAAATAGGCCATTCAAACCAAGGGAATAACATGTACCTTTTCCCTGGGATTGGACTTGGGACACTTCTTTCAGGTGCCCATATTATTTCAGATAGAATGTTACAAGCAGCTGCTGAGTGCCTTGCAGAGTATATGACAGATGCTGAGATCCAAAGAGGACTTGTTTTCCCTTCCATTTCAAGTATCCGTAATATCACGAAGAGAGTTGCAGCAGCAGTCGTGAAAGCAGCAGTTGCAGAAGACCTTGCAGATGGATATCGAGAAACAGGACCTAAGGAGCTCCGTCAGATGTCAATGGAGGAGACAGTGAAATATGTGGGAGATAATATGTGGTTTCCTGAATATTCACCTCTTGTGCTAGAAAAGTAA___GATCCTTAAGATGTTTTGCTAATAAAACTCTTAGGAATGTTGGACCCCATTTCAGAATGCCATTTGAAAAGATTCAAGGTGGTAGCTGTTGCCTGGGGCTGTTTTAGGGGTTTTAGGCCATGCTCTGTAATTTCATTGAGTCAAAATTGGATTAACTGGTGTCTTTTATCCTCATAATAGCTACTGCAGTATTTGTCGATATAGCTTCCCTATTTATTGACTCTCCTTGAGGTACTGTCTGCAGTTAAGAAAGCAAGTGCGGGCACTTCCATTTATGTATGTGACACAAAGTAACAGTTTTGATGTAGTACGATTTGGTAGTCAGATTACTTTTCTTCTACTTGTATGTTCACGTTTATATGTAATTGGTTGGAAGAACTAGGTCCAGTTTCTCATTCTCGGTCG

Fln Alignment:

Contig7259___RPCIVHKRGSDILHDPWFNKETAFSVTEXXXXXXXXXXXXRIISFQQQCDRFMVSFRSLEHNTRDGSADLTALAKWRILNRLHDRNETLYYKVLIDHIEEFAPIIYTPTVGLVCQNYGGLFRRPRGMYFSARDKGEMMSMIYNWPSEQVDMIVVTDGSRILGLGDLGVQGVAIPIGKLDMYVAAAGINPQRILPVMLDVGTNNVKLLNNSLYLGLRQPRLEGDEYLSVLDEFMEAVFTRWPQVIVQFEDFQTKWAFKLLQRYRKSFRMFNDDXXXXXXXXXXXXXXXXXXXXRPITDFVKQKXXXXXXXXXXXXVLNTAEQAMSRMLG--SNSLSSANCQFWLLDKSGLITHARKDLDPEAAPFARGFGPGEIEGLHEGASLVEVVKKVKPHVLLGLSGVGGIFSQEVLEAMRDSESPRPAIFAMSNPTENAECTAADAFKYAGENIVFASGSPFQDVDLGNGKIGHSNQGNNMYLFPGIGLGTLLSGAHIISDRMLQAAAECLAEYMTDAEIQRGLVFPSISSIRNITKRXXXXXXXXXXAEDLADGYRETGPKELRQMSMEETVKYVGDNMWFPEYSPLV
B8LMR7______________RPTIVHRRGLDILHDPLFNKGTAFPLAERDRLGLRGLLPPSVMTWKQQIDRFMVDLKRLEEHAKDGPSDTTALAKWRILNRLHDRNETLYYKVLIDNIAEYAQIIYTPTVGLVCQNYSGLYRRPRGMYFSTADQGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVAASGINPQRVLPVMIDCGTNNEKLLGDPLYLGLPIRRLDGEEYVSVIDEFMEAVFARWPNVIVQFEDFQSKWAFKLLQRYRNQYRMFNDDVQGTAGVAIAGLLGAVRAQGRPISDFAKQKIVVSGAGSAGIGVLNAARKAMARFLGGTESAFENARCQFWVLDAMGLVTEARNDIDPDVAPFARKVEELGSQGLREGSQLTEVVKKVRPDVLIGLTGIGGLFTKEVLEALKESTCVRPVVFAMSNPTSNAECTADQAFNTVGHHIVFASGSLFPDVDLGNGQIGHSNQGNNMYLFPGIGLGTLVSGARLISDGMLQAAAECLAECMTKEEVRKGIIYPSVSRIRNITMKVATAVVREAVAEDLAEGYQDKDARELQRLSKEETLEHVEKHMWVPNYSPLV

SNPs (tot: 46)

Position	A %	C %	G %	T %	Probability
666			88	11	0.923564
667		88		11	0.923564
736	87	12			0.992554
738	87			12	0.992554
739			87	12	0.992554
829		83		16	0.994512
973	87		12		0.992554
1001		87	12		0.992554
1002	12			75	0.975346
1003			12	87	0.992554
1004		12		87	0.992554
1005	12			87	0.992554
1008	87	12			0.992554
1009		87	12		0.992554
1010		87		12	0.992554
1012	12	87			0.992554
1014		12		87	0.992554
1104		85		14	0.999981
1114	14			85	0.999981
1269		10		90	0.90298
1314			88	11	0.923564
1398		83		16	0.994512
1406		25		75	0.996473
1407		25		75	0.996473
1408		25		75	0.996473
1409	25		75		0.996473
1410			75	25	0.996473
1515		80		20	0.995493
1780	87	12			0.992554
1786		87		12	0.992554
1885		14		85	0.993533
1886		14		85	0.993533
1887		14	85		0.993533
1888	14			85	0.993533
1889	85			14	0.993533
2277	8		92		0.999718
2283	87			12	1.0
2320		15	85		1.0
2322		15		84	1.0
2323		5	84		0.999846
2326			84	15	1.0
2397	12	87			0.992554
2399		14	85		0.993533
2403		25	50	25	0.995533
2404	25	25	50		0.995533
2405	25	50		25	0.995533

SSRs (tot: 1)

Start position	End position	Sequence	Length
1239	1250	CAA CAA CAA CAA	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain