SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene6711

Length: 2115 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene6711
TATTGAATGTCTGACTTTGGCTGTTGGTCTATTTCCAGTGGACAGGCCTAAAGAAGAACCCATCGAGTGTTCTCACGCGTAAGGTTCAACCACAGCCATGATTAAGTAGTCCACACATTGTATTATACACCACCCTCCCGCCCCTTCTTCCTTCCACCATGACATCGACGCTATCCTTTCTCTTTCCTTCTCTTAGCAGTAATTTAACTGTAAACCCTCAGTGTCCAAATTGTAATTCGTTTTTACCGAAGCATAAATCAAAATCTTATAGCTGGAACAGCAATAACAGCAGCAGGAACAAGATGTTGCGAAGAATTGAATGCAGCAGACGGGATGGGAAAGGTGGTTCAGGATCCCCTGATAAGGCAGGGGCTGGGCGTTCATGGGTGAGTCCGGATTGGCTGACTTCTTTTATGAGAGTAAGGCTATTAGGTCCTGATGCTTCTGGCATTCCCGTGGCAAGTGCAAAGCTAGACGATGTAAAAGACTTGTTGGGTGGCGCTCTCTTCCTTCCTCTTTTTAAATGGATGGAGGACTGCGGTCCTGTGTATAGATTAGCAGCTGGGCCTAGAAACTTCGTTGTAATCAGCGATCCTGAAGCAGCTAAGCATGTGTTAAGAAACTATGGCAAGTATGCAAAAGGGCTTGTGTCGGAGGTCTCTGAGTTCCTTTTGGTTCAGGTTTTGCCATTGCCGAGGGACAGCTGTGGACGGTTCGACGAAGGGCAGTTTTACCTTCACTCCATAGAAAATATCTTTCTGTAATCGTTGATAATGTTTTTTGCAAGTGTGCCGAGAGGCTAGTTGAGAAATTGAAGGCCAATATGGAAATGTCGGTTTCTGTTAACATGGAGGAAAAGTTTTCCCAGCTAACTCTAGACATTATTGGTTTATCATTATTCAATTACAACTTCAATTCACTTACATCGGACAGCCAACTCATCAATGCTGTTTACACTGCTCTGAAAGAGGCAGAATCTCGTTCAACTGATATTTTGCCATATTGGAAGATTGATCTTTTATGCAAGGTAATTCCGAGGCAAATAAAAGCAGCAAAGTCAGTGGCTATTATTAGAGAATCTGTTGAAGAACTAATAACCAAGTGCAAAGCAATAGTGGAAGCTGAGGGTGAACAACTAGAGGGAGAAGAGTATGTAAATGAATCAGACCCAAGTATTCTCCGGTTCTTATTGGCAAGTCGTGAAGAGGTCTCGAGTGTCCAGCTTCGGGATGATCTATTATCAATGCTAGTTGCTGGCCATGAGACCACGGCCTCTGTTTTAACATGGACAACATATCTGCTGAGCCAGAACCCTCATGCAATGGCAAGGGCACAAGAAGAAGTTGATAGAGTCCTACAAGGAAGGAAACCAAAATTTGCAGACGTCAAGGAGCTTAAGTACTTGACTCGTTGCATAAATGAGTCAATGCGACTTTATCCTCACCCACCTGTCTTGATTAGACGAGCTCAAGAGCCTGATATTTTGCCTGGAGGTTATAAGGTGGATGCTGGTCAGGATATTATGATTTCCATCTACAACATCCATCATTCACCAATGGTATGGGATAAGGCCGAAGAATTCATACCTGAAAGATTTGACTTGGATGGTCCGGTGCCAAATGAAACAAATTCGGACTTCAGATTCATTCCCTTTAGTGGTGGCCCACGTAAGTGCGTTGGTGATCAGTTTGCATTGCTGGAATCCCTTGTTGCACTGGCAATTTTACTGCAGAACTTTAATTTTGAGCTTGTTCCTAACCAAAACATAGGAATGACCACAGGAGCCACTATTCATACCACAACAGGACTTTACCATGACTGTGACTCAACGAGAAAATAAGGTTACAGAGCTTGCAACTTTGTAATAGGAGAAAACTCTTTTTCTATATTTCTGTTCCTATGGATTCCAAACTGTGTTCATCTCATTGGCCAGAATTATTAGTTATGAAAAGAAACCAAGGAAATTTAATTGATTTGGACTGCTGATACTATCAATGAGATCTGAGAAAATTTCTCTAACTCTGCATTGTACGCTGCAAGTTATCAAGTTTTGTCAACCAACAACTTTGGCTGTCTGTGACTTTCAGAAACTAAGAGAGAGAGAGAGAGAGAGTC

Ace file of the UniGene sp_v3.0_unigene6711 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	putative cytochrome P450 [Arabidopsis thaliana]	-	-	0.0	73%
FL-Next	sp=Carotene epsilon-monooxygenase, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	74%
Sma3	Lutein deficient 1-like protein	-	-	8.479e-08	-

Source	ECs	Term	Type	e value	Identity
Sma3		EC:1.14.-.-	-	1.188e-07	-
Sma3	Unspecific monooxygenase.	EC:1.14.14.1	-	4.218e-07	-

Source	KEGGs	Term	e value	Identity
Sma3	Fatty acid metabolism	00071	4.218e-07	%
Sma3	Steroid hormone biosynthesis	00140	4.218e-07	%
Sma3	Caffeine metabolism	00232	4.218e-07	%
Sma3	Tryptophan metabolism	00380	4.218e-07	%
Sma3	Arachidonic acid metabolism	00590	4.218e-07	%
Sma3	Linoleic acid metabolism	00591	4.218e-07	%
Sma3	Aminobenzoate degradation	00627	4.218e-07	%
Sma3	Retinol metabolism	00830	4.218e-07	%
Sma3	Metabolism of xenobiotics by cytochrome P450	00980	4.218e-07	%
Sma3	Drug metabolism - cytochrome P450	00982	4.218e-07	%
Sma3	Drug metabolism - other enzymes	00983	4.218e-07	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	4.218e-07	%
Sma3	Metabolic pathways	01100	4.218e-07	%

Source	Gene names
Sma3	At1g31800; At3g53130; At4g15110; CHLREDRAFT_196742; CHLREDRAFT_196744; CHXE; CRHE; CYP2; CYP97A10; CYP97A2; CYP97A5; CYP97A6; CYP97A7; CYP97B1; CYP97B2; CYP97B3; CYP97B7; CYP97C10; CYP97C4; CYP97E1; F5M6.19; GSVIVT00000577001; GSVIVT00025137001; LOC_Os10g

Source	GOs	Term	Type	Identity
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	carotene beta-ring hydroxylase activity	GO:0010291	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	xanthophyll biosynthetic process	GO:0016123	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Tropomyosin	IPR000533	-	-
Sma3	ATPase, F1 complex, OSCP/delta subunit	IPR000711	-	-
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Cytochrome P450, E-class, group IV	IPR002403	-	-
Sma3	Adenosine/AMP deaminase active site	IPR006650	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G53130.1	LUT1, CYP97C1 Cytochrome P450 superfamily protein chr3:19692812-19695278 FORWARD LENGTH=539	77%
RefSeq	Arabidopsis thaliana	NP_190881.2	carotenoid epsilon-ring hydroxylase [Arabidopsis thaliana]	77%
RefSeq	Populus trichocarpa	XP_002326389.1	cytochrome P450 [Populus trichocarpa]	76%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q6TBX7

Fln msg: Overlapping hits, possible frame ERROR between 671 and 671,

Fln protein: MTSTLSFLFPSLSSNLTVNPQCPNCNSFLPKHKSKSYSWNSNNSSRNKMLRRIECSRRDGKGGSGSPDKAGAGRSWVSPDWLTSFMRVRLLGPDASGIPVASAKLDDVKDLLGGALFLPLFKWMEDCGPVYRLAAGPRNFVVISDPEAAKHVLRNYGKYAKGLVSEVSEFxxGSGFAIAEGQLWTVRRRAVLPSLHRKYLSVIVDNVFCKCAERLVEKLKANMEMSVSVNMEEKFSQLTLDIIGLSLFNYNFNSLTSDSQLINAVYTALKEAESRSTDILPYWKIDLLCKVIPRQIKAAKSVAIIRESVEELITKCKAIVEAEGEQLEGEEYVNESDPSILRFLLASREEVSSVQLRDDLLSMLVAGHETTASVLTWTTYLLSQNPHAMARAQEEVDRVLQGRKPKFADVKELKYLTRCINESMRLYPHPPVLIRRAQEPDILPGGYKVDAGQDIMISIYNIHHSPMVWDKAEEFIPERFDLDGPVPNETNSDFRFIPFSGGPRKCVGDQFALLESLVALAILLQNFNFELVPNQNIGMTTGATIHTTTGLYHDCDSTRK*

Protein Length: 562

Fln nts: TATTGAATGTCTGACTTTGGCTGTTGGTCTATTTCCAGTGGACAGGCCTAAAGAAGAACCCATCGAGTGTTCTCACGCGTAAGGTTCAACCACAGCCATGATTAAGTAGTCCACACATTGTATTATACACCACCCTCCCGCCCCTTCTTCCTTCCACC_-_ATGACATCGACGCTATCCTTTCTCTTTCCTTCTCTTAGCAGTAATTTAACTGTAAACCCTCAGTGTCCAAATTGTAATTCGTTTTTACCGAAGCATAAATCAAAATCTTATAGCTGGAACAGCAATAACAGCAGCAGGAACAAGATGTTGCGAAGAATTGAATGCAGCAGACGGGATGGGAAAGGTGGTTCAGGATCCCCTGATAAGGCAGGGGCTGGGCGTTCATGGGTGAGTCCGGATTGGCTGACTTCTTTTATGAGAGTAAGGCTATTAGGTCCTGATGCTTCTGGCATTCCCGTGGCAAGTGCAAAGCTAGACGATGTAAAAGACTTGTTGGGTGGCGCTCTCTTCCTTCCTCTTTTTAAATGGATGGAGGACTGCGGTCCTGTGTATAGATTAGCAGCTGGGCCTAGAAACTTCGTTGTAATCAGCGATCCTGAAGCAGCTAAGCATGTGTTAAGAAACTATGGCAAGTATGCAAAAGGGCTTGTGTCGGAGGTCTCTGAGTTCxxxxxxGGTTCAGGTTTTGCCATTGCCGAGGGACAGCTGTGGACGGTTCGACGAAGGGCAGTTTTACCTTCACTCCATAGAAAATATCTTTCTGTAATCGTTGATAATGTTTTTTGCAAGTGTGCCGAGAGGCTAGTTGAGAAATTGAAGGCCAATATGGAAATGTCGGTTTCTGTTAACATGGAGGAAAAGTTTTCCCAGCTAACTCTAGACATTATTGGTTTATCATTATTCAATTACAACTTCAATTCACTTACATCGGACAGCCAACTCATCAATGCTGTTTACACTGCTCTGAAAGAGGCAGAATCTCGTTCAACTGATATTTTGCCATATTGGAAGATTGATCTTTTATGCAAGGTAATTCCGAGGCAAATAAAAGCAGCAAAGTCAGTGGCTATTATTAGAGAATCTGTTGAAGAACTAATAACCAAGTGCAAAGCAATAGTGGAAGCTGAGGGTGAACAACTAGAGGGAGAAGAGTATGTAAATGAATCAGACCCAAGTATTCTCCGGTTCTTATTGGCAAGTCGTGAAGAGGTCTCGAGTGTCCAGCTTCGGGATGATCTATTATCAATGCTAGTTGCTGGCCATGAGACCACGGCCTCTGTTTTAACATGGACAACATATCTGCTGAGCCAGAACCCTCATGCAATGGCAAGGGCACAAGAAGAAGTTGATAGAGTCCTACAAGGAAGGAAACCAAAATTTGCAGACGTCAAGGAGCTTAAGTACTTGACTCGTTGCATAAATGAGTCAATGCGACTTTATCCTCACCCACCTGTCTTGATTAGACGAGCTCAAGAGCCTGATATTTTGCCTGGAGGTTATAAGGTGGATGCTGGTCAGGATATTATGATTTCCATCTACAACATCCATCATTCACCAATGGTATGGGATAAGGCCGAAGAATTCATACCTGAAAGATTTGACTTGGATGGTCCGGTGCCAAATGAAACAAATTCGGACTTCAGATTCATTCCCTTTAGTGGTGGCCCACGTAAGTGCGTTGGTGATCAGTTTGCATTGCTGGAATCCCTTGTTGCACTGGCAATTTTACTGCAGAACTTTAATTTTGAGCTTGTTCCTAACCAAAACATAGGAATGACCACAGGAGCCACTATTCATACCACAACAGGACTTTACCATGACTGTGACTCAACGAGAAAATAA___GGTTACAGAGCTTGCAACTTTGTAATAGGAGAAAACTCTTTTTCTATATTTCTGTTCCTATGGATTCCAAACTGTGTTCATCTCATTGGCCAGAATTATTAGTTATGAAAAGAAACCAAGGAAATTTAATTGATTTGGACTGCTGATACTATCAATGAGATCTGAGAAAATTTCTCTAACTCTGCATTGTACGCTGCAAGTTATCAAGTTTTGTCAACCAACAACTTTGGCTGTCTGTGACTTTCAGAAACTAAGAGAGAGAGAGAGAGAGAGTC

Fln Alignment:

Contig6711___RSWVSPDWLTSFMRVRLLGP-DASGIPVASAKLDDVKDLLGGALFLPLFKWMEDCGPVYRLAAGPRNFVVISDPEAAKHVLRNYGKYAKGLVSEVSEFLFGSGFAIAEGQLWTVRRRAVLPSLHRKYLSVIVDNVFCKCAERLVEKLKANMEMSVSVNMEEKFSQLTLDIIGLSLFNYNFNSLTSDSQLINAVYTALKEAESRSTDILPYWKIDLLCKVIPRQIKAAKSVAIIRESVEELITKCKAIVXXXXXXXXXXXXXXESDPSILRFLLASREEVSSVQLRDDLLSMLVAGHETTASVLTWTTYLLSQNPHAMARAQEEVDRVLQGRKPKFADVKELKYLTRCINESMRLYPHPPVLIRRAQEPDILPGGYKVDAGQDIMISIYNIHHSPMVWDKAEEFIPERFDLDGPVPNETNSDFRFIPFSGGPRKCVGDQFXXXXXXXXXXXXLQNFNFELVPNQNI
Q6TBX7______________QSWVSPDWLTTLTRTLSSGKNDESGIPIANAKLDDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYPKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHRRYLSVIVERVFCKCAERLVEKLQPYAEDGSAVNMEAKFSQMTLDVIGLSLFNYNFDSLTTDSPVIEAVYTALKEAELRSTDLLPYWKIDALCKIVPRQVKAEKAVTLIRETVEDLIAKCKEIVEREGERINDEEYVNDADPSILRFLLASREEVSSVQLRDDLLSMLVAGHETTGSVLTWTLYLLSKNSSALRKAQEEVDRVLEGRNPAFEDIKELKYITRCINESMRLYPHPPVLIRRAQVPDILPGNYKVNTGQDIMISVYNIHRSSEVWEKAEEFLPERFDIDGAIPNETNTDFKFIPFSGGPRKCVGDQFALMEAIVALAVFLQRLNVELVPDQTI

SNPs (tot: 37)

Position	A %	C %	G %	T %	Probability
585	16		83		0.994512
586		16	83		0.994512
588	83			16	0.994512
589	83			16	0.994512
666	80			20	0.995493
667		25	75		0.996473
668	25		75		0.996473
670	25		75		0.996473
671		25	75		0.996473
672	25		75		0.996473
674		25	75		0.996473
689	25			75	0.996473
1100			87	12	0.992554
1116		12		87	0.992554
1117	12		87		0.992554
1118			87	12	0.992554
1119		87		12	0.992554
1143		33		66	1.0
1189	14		85		0.993533
1443	75	25			0.996473
1445	75	25			0.996473
1446	75	25			0.996473
1447	75		25		0.996473
1448	20	20	60		0.99255
1450	60		20	20	0.99255
1455	80			20	0.995493
1457	80	20			0.995493
1459	80			20	0.995493
1463		80	20		0.995493
1464	20	80			0.995493
1471	80			20	0.995493
1473		80		20	0.995493
1474	20	80			0.995493
1476	20			80	0.995493
1545			33	66	0.997452
1683			50	50	1.0
1787		80		20	0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
3	22	CT CT CT CT CT CT CT CT CT CT	20

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain