SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene5971

Length: 2300 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene5971
TGCATCAAATAAGTACCCATTTGATGAACATTCAATATTTTTCAAACTTCCAAGCTTAAAGCTCAGTTCAAGTTCCTACAGCCATCTCTGATCGAAGCTCTTGAGGCCTTGTTTGGCATTTTCTTGAATCCATTAACAGGCTGCTAAATCTTGAAAGCAAAAATGGGCAACTCTGATGATGGTTCTGAGATCCACGACGTTGAGGTAATCCATTCATGGTCATTACCAAGGTCTCTGAGCACCAGTCTCATGTACGCATTTGCACAGAGGGACGATACAGAAGTGATTGATGAACCTCTATATGCACATTTTGTCGCGGAAACTGGGGTGGACAGGCCTTATAAAGAAGAATTGCTGTACAAAAATGGAACCAGATGGGAACAAGGTGATTAAAGATGTAATTTTTGGGCCCGGAAAGAAGAAGTATAGATATTGCAAGCACATTGCAAAACAACGCCTGCCTTCTTTGGCAAAAGACTTGATGAAGAAGGGAAAACATTTCATACTTATAAGGAGTCCACTTGATGTTTTGCGATCGTTTGACAAGGTTGTGGCAGCTTCCTTCCTCGAGCTAGAATTGGCAAGCTTAGTGACAATATATGGTGAACTTTGTGAGCTTGGTTCTCCTCCAACAATTATCGATGCACTAGACTTGCAAAGGAATTCTGAGGGTACACTACGTTGTCTCTGCAATGCTCTGGAGATTCCTTTCCAAAGTTCTATGCTCAAATGGGAAGCTGGTCCAAAACCAGTGGATGGCCTTTGGGCTCCATGGTGGTATGCAAGTGTTCATAAGTCAACAGGTTTCAAGCCTCCATCAACATATCCAAAGCCTTTTCCATTCAAGCTTTATACTTTGTTGGAGCAATGCCTTCCTTTCTACAACTTACTGAGGCGCCATGCTCTACGGCCTGATGTGTCAGTGCAACCAACTCTTCCTGTTCCAACTAATGAGCAGATACTTGTTTGGGTTGGTGATGAGCTGCTTCCTCGTGAAGATGCAAAGGTATCTGTATTTGATTCAATTGTCCAAGGTGGTGATGGAGTTTGGGAGGGGCTTCGGGTCTACAATGGAAAAGTTTTCAAGTTGGACGAGCATTTGGACAGGTTGTCTGATTCTGCAAAAGCAATGGCTTTTGTAAATGTACCCGAACGTAATGAGATAAAGCGAGCAACTTTTACAACACTCATTTCAAATGGAATGCGAGATAATGCTCATATCAGGCTCACTTTGACCCGTGGGAAAAAGGTGACCTCAGGGATGAGCCCTGCATTCAATATGTATGGATGTACTTTGATAGTTCTGGCTGAGTGGAAGCCACCTGTCTATGACAATGCTGGTGGAATTTCCTTGATAACGGCTTCGACACGCCGCAATTCTCCAAATAACTTGGATTCCAAAATTCATCACAATAATCTTATCAATAACATTCTGGCAAAGATAGAGGGAAATCTTGCAAAGGCAGATGATGCAATCATGCTTGATCAATATGGTTTTGTATCTGAAACAAATGCTACAAATATTTTTTTAGTGAAGAAAGGGCAAGTGCATACTCCACGAGCTGATCACTGCTTACCAGGCATAACCCGTGCAACGGTAATTGATCTTGTGAAACAAGAAGGAATATATCTAGAAGAGCGTCAAATCAGCCTATCAGAGTTCCATACTGCAGACGAGGTGTGGACGACTGGCACAATGGGAGAGCTTACTCCAGTTGTGAAAATTGATGGTCGCACTATTGGAGAGGGAAACGTAGGCCCAATAACTTGTAGATTGCAACAAGCGTACAAAAAATTGGCAGAGTATGGAGAGCCAATACCAGAATAGTGCAGGATTGGGGAGATTTTATGGGCTTATGCATGTATCATCATTCTTCAATGAAGGAGGTGCTTTGTAGCTCCAGAAAGTTGTTCATAATAATATAAATGGTTCATGGTATACGGGGTATGTCAACTCCAAGCATACAGGGTTAGCAAGTGGCACTGATTTGATAATTCAATCAATGTGCTACTAGTTACAACCAGGAGCCCATCTTGCCAGCATTTATTAGACTTGTCTCAAATATCAAGCTCACATCCAATGTACTTAATTTAAGCATCTTGACAAGAATTGAAAGACAGCATATAAATTTAGGAGGCTTGATCCATTCTTATCAGTTGGAACTGTTTATTTATTATTTATCCCATTAACTTTTAAGGGAGACTGATTTGAAAAGATTTACTGGTATCATATCTTGCTCATTTTCTCCTATTTCCATTCAGATTTTTCGCAGGAAATGTGATAGCTATGGTCTAGAATA

Ace file of the UniGene sp_v3.0_unigene5971 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	branched-chain-amino-acid aminotransferase-like protein 2 [Arabidopsis thaliana] sp\|Q9ASR4.1\|BCAL2_ARATH RecName: Full=Branched-chain-amino-acid aminotransferase-like protein 2 gb\|AAK32910.1\|AF367323_1 AT5g27410/F21A20_120 [Arabidopsis thaliana] gb\|AAN180	-	-	0.0	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	26%
Sma3	Branched-chain amino acid aminotransferase, putative, expressed	-	-	7.196e-09	-

Source	ECs	Term	Type	e value	Identity
Sma3	Branched-chain-amino-acid transaminase.	EC:2.6.1.42	-	6.32e-09	-

Source	KEGGs	Term	e value	Identity
Sma3	Valine, leucine and isoleucine degradation	00280	6.32e-09	%
Sma3	Valine, leucine and isoleucine biosynthesis	00290	6.32e-09	%
Sma3	Pantothenate and CoA biosynthesis	00770	6.32e-09	%
Sma3	Glucosinolate biosynthesis	00966	6.32e-09	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	6.32e-09	%
Sma3	Biosynthesis of alkaloids derived from histidine and purine	01065	6.32e-09	%
Sma3	Metabolic pathways	01100	6.32e-09	%
Sma3	Biosynthesis of secondary metabolites	01110	6.32e-09	%

Source	Gene names
Sma3	At3g05190; At5g27410; BAT1; F21A20_120; GSVIVT00022872001; LOC_Os03g24460; Os03g0359100; OsI_11647; OsJ_10916; PHATRDRAFT_50806; PHYPADRAFT_130368; PHYPADRAFT_141539; POPTRDRAFT_595547; RCOM_1430610; RCOM_2072330; T12H1.16; THAPSDRAFT_33194; aat;

Source	GOs	Term	Type	Identity
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	branched-chain-amino-acid transaminase activity	GO:0004084	Molecular Function	-
Sma3	transaminase activity	GO:0008483	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	branched chain family amino acid metabolic process	GO:0009081	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Aminotransferase, class IV	IPR001544	-	-
Sma3	Branched-chain amino acid aminotransferase I	IPR005785	-	-
Sma3	Aminotransferase, class IV, conserved site	IPR018300	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G27410.1	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein chr5:9678821-9682468 FORWARD LENGTH=559	71%
RefSeq	Arabidopsis thaliana	NP_568496.1	branched-chain-amino-acid aminotransferase-like protein 2 [Arabidopsis thaliana]	71%
RefSeq	Populus trichocarpa	XP_002328541.1	predicted protein [Populus trichocarpa]	71%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PTG0

Fln msg: your sequence is longer than subject: 513 - 362

Fln protein: MNLYMHILSRKLGWTGLIKKNCCTKMEPDGNKVIKDVIFGPGKKKYRYCKHIAKQRLPSLAKDLMKKGKHFILIRSPLDVLRSFDKVVAASFLELELASLVTIYGELCELGSPPTIIDALDLQRNSEGTLRCLCNALEIPFQSSMLKWEAGPKPVDGLWAPWWYASVHKSTGFKPPSTYPKPFPFKLYTLLEQCLPFYNLLRRHALRPDVSVQPTLPVPTNEQILVWVGDELLPREDAKVSVFDSIVQGGDGVWEGLRVYNGKVFKLDEHLDRLSDSAKAMAFVNVPERNEIKRATFTTLISNGMRDNAHIRLTLTRGKKVTSGMSPAFNMYGCTLIVLAEWKPPVYDNAGGISLITASTRRNSPNNLDSKIHHNNLINNILAKIEGNLAKADDAIMLDQYGFVSETNATNIFLVKKGQVHTPRADHCLPGITRATVIDLVKQEGIYLEERQISLSEFHTADEVWTTGTMGELTPVVKIDGRTIGEGNVGPITCRLQQAYKKLAEYGEPIPE*

Protein Length: 514

Fln nts: TGCATCAAATAAGTACCCATTTGATGAACATTCAATATTTTTCAAACTTCCAAGCTTAAAGCTCAGTTCAAGTTCCTACAGCCATCTCTGATCGAAGCTCTTGAGGCCTTGTTTGGCATTTTCTTGAATCCATTAACAGGCTGCTAAATCTTGAAAGCAAAAATGGGCAACTCTGATGATGGTTCTGAGATCCACGACGTTGAGGTAATCCATTCATGGTCATTACCAAGGTCTCTGAGCACCAGTCTCATGTACGCATTTGCACAGAGGGACGATACAGAAGTGATTG_-_ATGAACCTCTATATGCACATTTTGTCGCGGAAACTGGGGTGGACAGGCCTTATAAAGAAGAATTGCTGTACAAAAATGGAACCAGATGGGAACAAGGTGATTAAAGATGTAATTTTTGGGCCCGGAAAGAAGAAGTATAGATATTGCAAGCACATTGCAAAACAACGCCTGCCTTCTTTGGCAAAAGACTTGATGAAGAAGGGAAAACATTTCATACTTATAAGGAGTCCACTTGATGTTTTGCGATCGTTTGACAAGGTTGTGGCAGCTTCCTTCCTCGAGCTAGAATTGGCAAGCTTAGTGACAATATATGGTGAACTTTGTGAGCTTGGTTCTCCTCCAACAATTATCGATGCACTAGACTTGCAAAGGAATTCTGAGGGTACACTACGTTGTCTCTGCAATGCTCTGGAGATTCCTTTCCAAAGTTCTATGCTCAAATGGGAAGCTGGTCCAAAACCAGTGGATGGCCTTTGGGCTCCATGGTGGTATGCAAGTGTTCATAAGTCAACAGGTTTCAAGCCTCCATCAACATATCCAAAGCCTTTTCCATTCAAGCTTTATACTTTGTTGGAGCAATGCCTTCCTTTCTACAACTTACTGAGGCGCCATGCTCTACGGCCTGATGTGTCAGTGCAACCAACTCTTCCTGTTCCAACTAATGAGCAGATACTTGTTTGGGTTGGTGATGAGCTGCTTCCTCGTGAAGATGCAAAGGTATCTGTATTTGATTCAATTGTCCAAGGTGGTGATGGAGTTTGGGAGGGGCTTCGGGTCTACAATGGAAAAGTTTTCAAGTTGGACGAGCATTTGGACAGGTTGTCTGATTCTGCAAAAGCAATGGCTTTTGTAAATGTACCCGAACGTAATGAGATAAAGCGAGCAACTTTTACAACACTCATTTCAAATGGAATGCGAGATAATGCTCATATCAGGCTCACTTTGACCCGTGGGAAAAAGGTGACCTCAGGGATGAGCCCTGCATTCAATATGTATGGATGTACTTTGATAGTTCTGGCTGAGTGGAAGCCACCTGTCTATGACAATGCTGGTGGAATTTCCTTGATAACGGCTTCGACACGCCGCAATTCTCCAAATAACTTGGATTCCAAAATTCATCACAATAATCTTATCAATAACATTCTGGCAAAGATAGAGGGAAATCTTGCAAAGGCAGATGATGCAATCATGCTTGATCAATATGGTTTTGTATCTGAAACAAATGCTACAAATATTTTTTTAGTGAAGAAAGGGCAAGTGCATACTCCACGAGCTGATCACTGCTTACCAGGCATAACCCGTGCAACGGTAATTGATCTTGTGAAACAAGAAGGAATATATCTAGAAGAGCGTCAAATCAGCCTATCAGAGTTCCATACTGCAGACGAGGTGTGGACGACTGGCACAATGGGAGAGCTTACTCCAGTTGTGAAAATTGATGGTCGCACTATTGGAGAGGGAAACGTAGGCCCAATAACTTGTAGATTGCAACAAGCGTACAAAAAATTGGCAGAGTATGGAGAGCCAATACCAGAATAG___TGCAGGATTGGGGAGATTTTATGGGCTTATGCATGTATCATCATTCTTCAATGAAGGAGGTGCTTTGTAGCTCCAGAAAGTTGTTCATAATAATATAAATGGTTCATGGTATACGGGGTATGTCAACTCCAAGCATACAGGGTTAGCAAGTGGCACTGATTTGATAATTCAATCAATGTGCTACTAGTTACAACCAGGAGCCCATCTTGCCAGCATTTATTAGACTTGTCTCAAATATCAAGCTCACATCCAATGTACTTAATTTAAGCATCTTGACAAGAATTGAAAGACAGCATATAAATTTAGGAGGCTTGATCCATTCTTATCAGTTGGAACTGTTTATTTATTATTTATCCCATTAACTTTTAAGGGAGACTGATTTGAAAAGATTTACTGGTATCATATCTTGCTCATTTTCTCCTATTTCCATTCAGATTTTTCGCAGGAAATGTGATAGCTATGGTCTAGAATA

Fln Alignment:

Contig5971___VSVFDSIVQGGDGVWEGLRVYNGKVFKLDEHLDRLSDSAKAMAFVNVPERNEIKRATFTTLISNGMRDNAHIRLTLTRGKKVTSGMSPAFNMYGC------TLIVLAEWKPPVYDNAGGISLITASTRRNSPNNLDSKXXXXXXXXXXLAKIEGNLAKADDAIMLDQYGFVSETNATNI-FLVKKGQVHTPRADHCLPGITRATVIDL---VKQEGIYLEER--QISLSEFHTADEVWTTGTMGELTPVVKIDGRTIGEGNVGPITCRLQQ
C0PTG0______________IPIDDHMVHRGHGVFDTAMIIDGYLYELDSHLDRFLRSASKAKVIPPFDRSSVRSILLQTVAASQCRKGS-LRYWLSAGPG-DFLLSPA----GCPNSAFYAIVIEDDYSP----SSQGVTVITSSTPMKSPEMATMK--NVNYLPNVLSKMEAEEKGAFAAIWIDDQDYIAEGPNVNVAFISKNNELLLPSFDKILSGCTAKRLLAVAATLVEQGLLKNIRVGNITLDEGKGAAEMMLVGSGLPILPVTGWDGKPIGNGKEGPLTLALSK

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
1615		11	88		0.923564
1617		88		11	0.923564
1618	88			11	0.923564
1677		16	83		0.994512
1679	83		16		0.994512
1685		83	16		0.994512
1690	83		16		0.994512
1692	85	14			0.993533
1815		20		80	0.995493
1857	75			25	0.996473
1951			20	80	0.995493
1952		80		20	0.995493
1978			66	33	0.997452
2029	33		66		0.997452
2031		66		33	0.997452
2065		66	33		0.997452
2135	20	80			0.995493
2136	20	80			0.995493
2137		80		20	0.995493
2138	80		20		0.995493
2242	20			80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain