SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene5806

Length: 1838 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene5806

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	putative mitogen-activated protein kinase 1 [Medicago sativa]	-	-	0.0	63%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	49%
Sma3	Mitogen activated protein kinase kinase kinase 3, mapkkk3, mekk3, putative	-	-	2.898e-13	-

Source	ECs	Term	Type	e value	Identity
Sma3	Transferred entry: 2.7.11.1.	EC:2.7.1.37	-	6.064e-11	-
Sma3	Mitogen-activated protein kinase kinase kinase.	EC:2.7.11.25	-	1.868e-17	-

Source	Gene names
Sma3	ANP1; ANP2; ANP3; AT4g08480; At1g09000; At1g53570; At1g54960; At1g63700; At3g06030; At3g07980; At3g13530; At4g08470; At4g08480; At4g08500; At4g12020; At5g66850; B0103C08-B0602B01.10; CHLREDRAFT_105035; CHLREDRAFT_121660; CHLREDRAFT_98709; DUPR11.32; F14C2

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	cytosol	GO:0005829	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	intrinsic to membrane	GO:0031224	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	sequence-specific DNA binding transcription factor activity	GO:0003700	Molecular Function	-
Sma3	protein kinase activity	GO:0004672	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	MAP kinase kinase kinase activity	GO:0004709	Molecular Function	-
Sma3	protein tyrosine kinase activity	GO:0004713	Molecular Function	-
Sma3	transmembrane signaling receptor activity	GO:0004888	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	nucleoside-triphosphatase activity	GO:0017111	Molecular Function	-
Sma3	kinase binding	GO:0019900	Molecular Function	-
Sma3	sequence-specific DNA binding	GO:0043565	Molecular Function	-
Sma3	MAPK cascade	GO:0000165	Biological Process	-
Sma3	cytokinesis	GO:0000910	Biological Process	-
Sma3	regulation of transcription, DNA-dependent	GO:0006355	Biological Process	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	apoptotic process	GO:0006915	Biological Process	-
Sma3	response to oxidative stress	GO:0006979	Biological Process	-
Sma3	plasma membrane organization	GO:0007009	Biological Process	-
Sma3	signal transduction	GO:0007165	Biological Process	-
Sma3	response to cold	GO:0009409	Biological Process	-
Sma3	pollen development	GO:0009555	Biological Process	-
Sma3	response to wounding	GO:0009611	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	flower development	GO:0009908	Biological Process	-
Sma3	innate immune response	GO:0045087	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Armadillo	IPR000225	-	-
Sma3	HECT	IPR000569	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	NB-ARC	IPR002182	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	AAA+ ATPase domain	IPR003593	-	-
Sma3	DNA-binding WRKY	IPR003657	-	-
Sma3	Paired amphipathic helix	IPR003822	-	-
Sma3	Ribosomal protein S12/S23	IPR006032	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Twin-arginine translocation pathway, signal sequence	IPR006311	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Leucine-rich repeat 3	IPR011713	-	-
Sma3	Armadillo-like helical	IPR011989	-	-
Sma3		IPR015748	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Transcription regulator, HTH DeoR-type, conserved site	IPR018356	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G08500.1	MEKK1, ATMEKK1, MAPKKK8, ARAKIN MAPK/ERK kinase kinase 1 chr4:5404272-5407062 REVERSE LENGTH=608	51%
RefSeq	Arabidopsis thaliana	NP_192590.1	mitogen-activated protein kinase kinase kinase 1 [Arabidopsis thaliana]	51%
RefSeq	Populus trichocarpa	XP_002302260.1	predicted protein [Populus trichocarpa]	52%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LR19

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 94 aas, your sequence is shorter than subject: 516 - 902

Fln protein: SDWEIRKTKSRCSSLGNEGISQEIKDLSREIEGQTHNIRSLSPPEVSSPKGNGSAAVSPPLLSETPSVPASDSPGESEVGGPEISGLEGGRKLVVSVRTRGDHCRIKPLSEAAATSACVQSFTLAPPPSMSLKVSNDKGGSTWDILKSLAPEEDGICLPSTDRALTPNSQESDDGSDSDEEFEEEKQEDDRGAEVKRGRSWVLSSSSSGGESFTSSTEKAVYTVSKDGKFKVRFDSWIKGNMLGRGSFGTVYEGISDTGFFFAVKEVSLVEQGLGAKQCICQLEQEIALLSQLEHKNIVQYLGTDQEPEKLYIFLELVTQGSLQSLYQKYRLIDFQARAYTRQILIGLKYLHDRNVIHRDIKCANILVDTHGVIKLADFGMAKEISKLNELKSCKGSAYWMAPEVINPRKSYWLPADIWSLGCTVLEMLTRRPPNGDLEWHRALWKVGHGEIPPIPNALSSDAKDFIKRCLKIEAADRPSAAELLDHPFVKRSLSADVSTAEPSTKSRRQRPLAR*

Protein Length: 517

Fln nts: CCTCCGATTGGGAAATTCGAAAGACTAAATCGAGATGCAGCTCTTTGGGAAATGAAGGGATTTCTCAGGAAATTAAAGACCTTTCTCGGGAAATTGAAGGGCAGACGCACAACATTAGGTCATTGTCTCCCCCAGAAGTCTCCAGCCCCAAAGGAAACGGGTCCGCTGCCGTGTCCCCGCCTTTGCTCTCAGAGACTCCGTCTGTGCCGGCATCCGATAGCCCCGGAGAGAGCGAAGTCGGGGGTCCTGAAATAAGCGGGTTGGAGGGTGGCCGCAAGCTTGTGGTTTCGGTTCGCACAAGAGGAGACCATTGTAGGATTAAACCCCTTTCTGAGGCCGCGGCTACTTCGGCTTGTGTCCAATCGTTTACCCTGGCTCCGCCGCCGTCGATGTCTTTGAAGGTTTCGAATGACAAGGGTGGTTCGACTTGGGATATTTTGAAGTCTTTGGCTCCCGAGGAGGATGGCATTTGTTTGCCTTCAACTGACAGGGCTTTGACGCCAAATTCGCAGGAGAGTGATGATGGTAGTGACAGTGATGAGGAATTTGAGGAGGAAAAGCAAGAGGATGATAGGGGTGCAGAGGTGAAAAGGGGCAGATCGTGGGTATTGTCGTCGAGCTCAAGCGGAGGTGAGTCATTTACGTCGTCAACAGAGAAGGCCGTCTATACCGTTTCCAAGGATGGGAAATTCAAGGTTCGGTTCGATTCTTGGATTAAAGGGAATATGCTCGGGCGGGGATCATTTGGAACTGTTTACGAGGGAATTAGCGATACTGGATTCTTCTTTGCAGTTAAGGAGGTTTCTCTTGTAGAGCAAGGTCTTGGTGCAAAACAGTGCATTTGTCAGCTGGAACAGGAGATTGCTCTTCTAAGTCAACTTGAACATAAAAACATCGTTCAGTATCTTGGCACCGACCAGGAACCTGAAAAGTTGTACATTTTCCTTGAGCTGGTAACACAGGGTTCACTACAATCACTTTACCAAAAATACCGCTTAATTGATTTTCAAGCTCGTGCATATACAAGACAAATACTAATTGGACTAAAGTACCTTCACGATAGAAATGTCATTCACAGAGACATTAAGTGCGCTAATATATTGGTGGATACCCATGGCGTAATCAAACTTGCAGATTTTGGGATGGCCAAAGAGATATCCAAGTTGAATGAATTAAAGTCGTGCAAAGGAAGTGCTTATTGGATGGCTCCTGAGGTTATTAATCCTCGAAAATCATACTGGCTTCCTGCTGACATATGGAGCTTAGGTTGCACTGTGTTGGAGATGCTAACTCGGAGACCTCCCAATGGTGATTTAGAATGGCATCGAGCCTTGTGGAAAGTTGGACACGGGGAGATACCACCAATACCGAATGCACTATCTAGTGATGCCAAGGACTTTATAAAACGTTGTTTAAAAATTGAGGCTGCAGATCGACCATCAGCAGCCGAGCTCCTGGATCATCCTTTTGTGAAACGGTCACTTTCAGCAGACGTGTCCACTGCTGAGCCTTCCACTAAAAGTAGACGCCAGAGACCTCTAGCAAGATGATTTTGAAAAAGGTGATATGTTATGTACATTTTCAATCATTGGTGAGTCTTTCCTGTCAAAACTTTGATGGGGTCTTAGCAGTGCCATTTTTGTACATTTGGGCTAAATACCTTGTATGCTTGTTTATTTGAGAAAATTTTCCAATAATGATTTCTAGATGGAAGATATCTATATTACTTTTCCTTGCTTCCTGCCTGTGCCCTTAAAAAGGGCACAAAGGTAAGTTCTTGTACGCCAGAATCTGCTTCTTACAGCTGGATTTTGTAAGCTTTAGTTCGTTTATTATCC

Fln Alignment:

Contig5806___WIKGNMLGRGSFGTVYEGIS-DTGFFFAVKEVSLVEQGLGAKQCICQLEQEIALLSQLEHKNIVQYLGTDQEPEKLYIFLELVTQGSLQSLYQKYRLIDFQA-RAYTRQILIGLKYLHDRNVIHRDIKCANILVDTHGVIKLADFGMAKEISKLNELKSCKGSAYWMAPEVINPRKS-YWLPADIWSLGCTVLEMLTRRPPNGDLEWHRALWKVGHGEIPPIPNALSSDAKDFIKRCLKIEAADRPSAAELLDHPFVKRSLSADVSTAEPSTKS
B8LR19______________WQKGKLLGCGTFGTVYVGFNRETGDMCAMKEVPLVPDDSKSSESIKQLEQEINLLSGLEHPNIVQYYGSETVEDLFYIYLEYVPGGSIYKLVNDYGPLEEPVIRIYTRQILSGLAYLHSMNTVHRDIKGGNILVDTYGRVKLADFGMAKHINGPATPLSLKGSPYWMAPEVLMQKNTGHDLAVDIWSLGCTVIEMATGKPPWSEYEGAAAMFKVFKSEVPPIPDSLSPEGRHFVQCCLCRNPAQRPKASQLLEHLFVRNATQQDSSDLLASATS

SNPs (tot: 58)

Position	A %	C %	G %	T %	Probability
532	25		75		0.996473
536		75		25	0.996473
642	14			85	0.993533
643	14			85	0.993533
645	85		14		0.993533
646		85		14	0.993533
708	33		66		1.0
719			33	66	1.0
726	50		33		1.0
728	33			66	1.0
730			33	66	1.0
731	33		66		1.0
739	33		50		1.0
762	40		60		1.0
768	60			40	1.0
826			50	50	1.0
828		50	50		1.0
847		33	66		0.997452
927		66		33	1.0
992	60			40	1.0
1009		20		80	0.995493
1013	60			40	1.0
1015		80		20	0.995493
1029	80		20		0.995493
1042		28		71	1.0
1068	66		33		1.0
1076			33	66	1.0
1096		71	28		1.0
1113	57		42		1.0
1122		28	71		1.0
1128	85		14		0.993533
1146	87			12	0.992554
1159		12		87	0.992554
1186			83	16	0.994512
1188	83	16			0.994512
1195		16	83		0.994512
1198		83		16	0.994512
1231	16		83		0.994512
1243		14	85		0.993533
1244			85	14	0.993533
1326	14	85			0.993533
1329		85	14		0.993533
1330			85	14	0.993533
1408	83			16	0.994512
1409	83		16		0.994512
1410		83		16	0.994512
1458	16	83			0.994512
1466	20			80	0.995493
1479		75		25	0.996473
1483	25	75			0.996473
1496		75		25	0.996473
1501		75		25	0.996473
1507	25	75			0.996473
1515		25		75	0.996473
1522	75		25		0.996473
1531	25		75		0.996473
1615		66		33	0.997452
1616			33	66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain