SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene5754

Length: 1588 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene5754 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Allene oxide synthase n=1 Tax=Camellia sinensis RepID=C7BBB6_CAMSI	-	-	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	63%
Sma3	Allene oxide synthase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Hydroperoxide dehydratase.	EC:4.2.1.92	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	alpha-Linolenic acid metabolism	00592	%
Sma3	Biosynthesis of plant hormones	01070	%
Sma3	Metabolic pathways	01100	%

Source	Gene names
Sma3	AOS; AOS1; AOS2; At5g42650; CYP74; CYP74A; CYP74A1; CYP74A2; CYP74A3; CYP74A7; CYP74C6v1-1; CYP74C6v1-2; CYP74C7-1; CYP74C7-2; CYP74C8; GSVIVT00014710001; GSVIVT00036454001; GSVIVT00036455001; GSVIVT00036456001; GSVIVT00036457001; HPL; HPL1; LOC_Os03g1250

Source	GOs	Term	Type	Identity
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast thylakoid membrane	GO:0009535	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	plastoglobule	GO:0010287	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	allene oxide synthase activity	GO:0009978	Molecular Function	-
Sma3	lyase activity	GO:0016829	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	hydroperoxide dehydratase activity	GO:0047987	Molecular Function	-
Sma3	response to fungus	GO:0009620	Biological Process	-
Sma3	jasmonic acid biosynthetic process	GO:0009695	Biological Process	-
Sma3	response to jasmonic acid stimulus	GO:0009753	Biological Process	-
Sma3	oxylipin biosynthetic process	GO:0031408	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Cytochrome P450, E-class, group IV	IPR002403	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G42650.1	AOS, CYP74A, DDE2 allene oxide synthase chr5:17097803-17099359 REVERSE LENGTH=518	47%
RefSeq	Arabidopsis thaliana	NP_199079.1	allene oxide synthase [Arabidopsis thaliana]	47%
RefSeq	Populus trichocarpa	XP_002302453.1	cytochrome P450 allene oxide synthase [Populus trichocarpa]	45%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKD1

Fln msg:

Fln protein: METSSAAAPERSPVMEIPGTYGLSFIGAILDRFEYFKGTDKFFERRVEKYKSTVFRVNMPPGPPFFSYNRVIILLDGKSFPVLYDLSKVDKSYLAGSYMPNLKFTGGYRPTIFMDPSCEKHHRLKRWCLDLLCRNRERNFPEFAKVFDELSVALENEMASSGKASFSDQMDQLLCNFLFRSIAGAYPLAPGPASLGTDGPSCIIKWVASVVGPTISTGVLPKLLDEFTIHLFPLPFFLVSKYYHKLHNFLWMNATSALDVAEKQFGLNREEACNNLVFYIAFNAFGGLRVYFTSMLKYIADAGGKLHTELAEEVRGAVEAHGGLNGKALQSMALVRSTAYEVMRIEPPVPLQYGKAKEDFFVESHGGCYAVNKGDMLCGYQPFATRDPKLFYMAEKFVPRRFMGEEGEKMLKHLL*SNGRETEETSADNKQCAGKDIVGTMGCLFLAHFFVRYDSFAIEKSSSSSVTLTMPTKGFLF*

Protein Length: 479

Fln nts: TCATGCCCTGTCTGTGCTATATAGTCGCTACCTTCCACCACAGCACAGCACAAGCACATTAGGTTCGCAAAGGGTTCTTTACCATATTTCAGTAAGTTTAGAGACCGGCAAGACG_-_ATGGAGACATCAAGCGCGGCCGCACCAGAGCGGTCTCCTGTAATGGAGATCCCTGGAACTTATGGCCTTTCCTTTATTGGAGCCATCTTGGATCGATTCGAATATTTCAAAGGAACAGATAAATTCTTCGAACGTCGTGTGGAGAAATACAAGAGCACAGTGTTCAGAGTGAATATGCCGCCTGGTCCGCCTTTCTTCTCGTACAATCGAGTCATAATATTGCTAGATGGTAAGAGCTTCCCCGTGCTCTACGATTTGAGTAAGGTGGATAAGAGTTATCTGGCGGGATCTTACATGCCCAACCTCAAATTCACCGGCGGATACAGGCCTACCATTTTTATGGACCCATCTTGTGAAAAACATCACAGGCTTAAGCGCTGGTGCCTTGATTTACTCTGCAGAAATCGAGAGAGAAATTTTCCAGAGTTTGCCAAGGTGTTCGATGAGCTCAGCGTGGCCCTCGAGAATGAAATGGCGAGTAGTGGGAAGGCAAGTTTCAGTGATCAGATGGATCAATTGCTGTGTAATTTTCTTTTCCGGTCCATTGCCGGCGCTTACCCGTTGGCTCCCGGGCCAGCCAGCCTCGGAACCGATGGTCCTTCCTGTATCATAAAGTGGGTCGCTTCTGTGGTCGGTCCTACCATAAGCACAGGCGTCCTTCCGAAATTGTTGGACGAGTTCACCATCCATTTATTTCCTCTGCCCTTCTTCCTCGTGTCCAAGTATTACCACAAACTCCATAATTTCCTGTGGATGAATGCGACTTCCGCGCTGGATGTGGCCGAAAAGCAGTTCGGCCTGAACAGAGAAGAGGCCTGCAATAATCTTGTGTTTTACATCGCTTTCAACGCCTTCGGGGGACTGCGCGTTTATTTTACCAGTATGCTCAAATATATTGCCGACGCCGGCGGCAAATTGCACACAGAACTGGCCGAAGAGGTCCGCGGAGCTGTTGAAGCACACGGGGGACTCAATGGCAAGGCTCTCCAGAGTATGGCCTTGGTTCGGTCTACTGCGTACGAAGTGATGAGGATAGAGCCTCCTGTGCCATTGCAGTACGGGAAAGCCAAGGAAGATTTCTTCGTCGAATCCCACGGCGGGTGTTATGCGGTGAATAAGGGCGATATGTTGTGCGGGTACCAACCGTTTGCAACCCGGGATCCCAAACTGTTCTACATGGCAGAAAAGTTCGTTCCGCGACGATTCATGGGCGAGGAAGGGGAGAAAATGCTGAAGCACCTGCTGTGATCCAATGGCCGTGAAACAGAAGAAACGTCCGCGGACAACAAGCAGTGTGCGGGGAAGGATATCGTTGGCACTATGGGTTGCCTCTTTCTTGCGCACTTCTTCGTGCGATATGATTCATTTGCCATAGAAAAGTCGTCTTCCTCGTCTGTCACACTTACGATGCCCACTAAGGGCTTTCTCTTCTAA___AATTTTTATCATGTAAGTTGCAAAGTAGATTTCAAATTT

Fln Alignment:

Contig5754___METSSAAAPERSPVMEIPGTYGLSFIGAILDRFEYF--KGTDKFFERRVEKYKSTVFRVNMPPGPPFFSYNRVIILLDGKSFPVLYDLSKVDK-SYLAGSYMPNLKFTGGYRPTIFMDPSCEKHHRLKRWCLDLLCRNRERNFPEFAKVFDELSVALENEMASSGKASFSDQMDQLLCNFLFRSIAGAYPLAPGPASLGTDGPSCIIKWVASVVGPTISTGVLPKLLDEFTIHLFPLPFFLVSKYYHKLHNFLWMNATSALDVAEKQFGLNREEACNNLVFYIAFNAFGGLRVYFTSMLKYIADAGGKLHTELAEEVRGAVEAHGGLNGKALQSMALVRSTAYEVMRIEPPVPLQYGKAKEDFFVESHGGCYAVNKGDMLCGYQPFATRDPKLFYMAEKFVPRRFMGEEGEKMLKHLL*SNGRETEETSADNKQCAGKDIVGTMGCLFLAHFFVRYDSFAIEKSSSS
B8LKD1______________MKVLASMASTAGPVKEIPGSYGPPLLGAIADRFEYFVTEGVDKFFKKRIDKYKSTVLKVNMPPGPLIVSDSRVIMLLDGKSFPVLYDLSKVEKKNVLTGTYMPSTSFTGGYRVAVYLDPSEENHSKLKRFCFEVLGSSRDRYFPEFARAFDELSAAVDKEMASRGKASFGEQIEQLIFNFLCRSLTGADPVTQG---LGTDGPSYVIKWLAPQLAPTLSSGFLPKIVDELTIHSIRLPFWPVSGYYDKLFNFLWTHAAPALDVAEKEFGLKRAEACHDLLFNISFNAFGGMSILFPSIVKYIGNAGNQLQRDLAEEIRGAVRAQGNLNGRALESMALVRSTVYEVLRIDPPVPLQYARAKTDFVVESHDGVYGVKKGELLGGYQPFATKDPKLFDRADEFVPRRFMGPEGEKMLKHVLWSNGRETDETSADNKQCGGKDIVVMVARLFVAHFFLQYDSYTIDQSSSS

SNPs (tot: 28)

Position	A %	C %	G %	T %	Probability
969			81	18	0.999994
1096	14		85		0.993533
1098			85	14	0.993533
1109	16			83	0.994512
1110		16	83		0.994512
1146	20		80		0.995493
1156		20	80		0.995493
1170		20	80		0.995493
1172		40		60	1.0
1186			33	66	1.0
1249		10		90	0.90298
1258			10	90	0.90298
1340		85		14	0.993533
1341			85	14	0.993533
1360			33	66	1.0
1372		16		83	0.994512
1453			83	16	0.994512
1464	66		33		1.0
1477		80	20		0.995493
1512	20		80		0.995493
1521			80	20	0.995493
1535		20	60	20	0.99255
1537		40		60	1.0
1538		40		60	1.0
1539	40		60		1.0
1544		33	66		0.997452
1545		33		66	0.997452
1546		33	66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain