SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene5736

Length: 2775 nt

UniGene Fasta

>sp_v3.0_unigene5736
ATGGGGCAATTAGGTAAGAGCGAGTCCGAGTTTGGTGAAAGGCCTTCATTAAACCCCCGACGCCCACGCCCACCATGGGGACCGCTACACGGCCATGATTGCGGGGATTGAAAGCTTCGCCTTGGTCTCAACGACTCACTGCGGTCCTGATACCACCTAACTGTACAAGCTGAAGTCCCCTCTCGCCCTCTCCTTGCTGCTGCAAAGATCTCGGGCGGTGCCTCTGTGGACAATTGAGTCAACAGGCTTCCAGGAGGTTAGGATAGTCTTATTTATCTCAAATCTAAAAGCAAAATCCAGAGAGGACACGAAATTCTGTTTTTGAACCGAGATTTGAGCTTTAAAATTAGATTTTATGGCCACCGATACCATGGTAGCGGATCCATACATGGACTCCTCAAGCGAATGCTCGGAATCTGAAGATGAAGGTACCGACGATTACAGGAAGGGAGGATACCATGCAGTTCGTTTGGGCGATTCGTTCAAGAATGGAAGATATATTGTGCAGCGAAAGCTCGGATGGGGTCATTTTTCTACTGTGTGGCTTGCTTGGGACCTCGAAGAAGCTAGATTTGTGGCTCTTAAGGTGCAGAAAAGTGCGCAACATTATGGAGAAGCAGCTATGGATGAGATCAACATCTTGAAGGAAATTGCAGAAGGAGATCCGGAAGATAGAAAAGGTGTTGTGAAATTGCTAGACCATTTCAAGCACGTTGGACCCAATGGGCAGCATGTTTGTATGGTTTTTGAGTATTTGGGTGACAGTTTGTTGTCATTGATAAAATATACTCGCTACCGAGGCCTTCCCTTGGACATTGTTCGGGAACTCTGCACCCCTATTTTAGTAGGATTGGATTATTTGCATCGAGAATTGTCAATTATTCACACTGATTTGAAACCTGAGAACATTCTTCTCTACTCTAATATCGATCCATCAAAAGATCCAAAGAACTCCAAGGAACCTCCCCTGCTTTCTCCTATTATAGATAAACCAAACCTACTTTTGAGGTCTTCCAGTACGAAAGACACAGTGAATGGAGCAATTTCAAGGACTCAGAAAAATAAATTAAAGAGAAAGGCTAAGAGGGCTGCAAACAACTGTGTGAGCAGTGTAATAGCTCGAGAAAAGGAATTAGATTCGGCTAATACAGACCACGGTAGTATAAGCAGTGGACAAACTGGTGTTTTAAACGCAGAAATCATTCTGGCTGGTGACTCAGCAGCTCAGCATGAGGCAGACAAAAAAGCTCGTTATTCTGCCAAGATTCAGAGCCTCAAATCAATAGATTTGAGATGTAAAATAGTAGACTTGGGAAATGCCTGCTGGATTCATAAGCAGTTTACACCTGATATTCAGACACGGCAATATCGATCCCCAGAGGTTATTTTGGGTTCAAAGTATTCTACACCAGTTGACATGTGGTCATTTGCTTGCATAATTTTTGAACTGGCTACAGGTGATATGTTATTTGATCCTCAGAGTGCAGAAGGTTATGACCGCGATGAGGACCACCTTGCCCTGATGATGGAGCTTCTTGGAAAAATACCTCGTAAGATAGCCTTAGGTGGGAGCTATTCACGGGAACTATTTGACAGGCATGGGGATTTAAAGCACATTAGACGGCTTCGGTATTGGCCCTTGGATCGTATACTAGTCGAGAAATATAAGTTCAGTGAGAAAGATGCTGATGAACTTGCCAAATTTTTATGCCCATTAATGGATTTTGCTCCAGAGAAGAGGCCTACAGCAGCGCAATGCCTCCTCCACCCATGGCTTAAATGCAAGATGGAAGACACAAGGGATAGACAAAGAAGGGAGAGTGAAGGTGCAAAGGAGGTCATGGAGAAAGCTCAAGTTGCTATGAGTAATATGGCTATCAAAGGAACTCAGGCAGGGTTCAGCAGAACAGCAGTGCCAATCAGGGCAAGCAATCAGCAAACAAGCACAATGCTCAAGTGAGTAGATTTAAGAAAAAGTCTGGTCTTCTAGAGTTATCGTCATAAGGATGCCTCCAGGTAGATACAGAACTGTACATTGCCATGCTGGTCGTCTTTCAAAGAATAACTCATTATATATTTCATGCGTGGGTATTAATTTAGGATGATTGATTTTATGGAGGCCTATTTCAAACAATCGTACCTACAAGTGAGTTTCAGTTACAAGATTGGTCTCGTTGTTTCAGAATCTAAAGAGCTGGAGTGTCCATCTCGACCCCCATCTTTGATTATATCTGTCTCAACTTATCAAGAACTTAAAAAGGTTCAAGGCCTTGGTGCCATGATTGCAACTCTTTGTCTGGAGTTCAATGTTTAGATGATGTCAAAATTGTTACTCCTTGTCCATAGGATGCTCTGATTTATGGTAAGATTTAGTACAGGATGGTTTCTATTGGTTATACAGCGGTTCACTCTGATTTGTAAAAAAGAGCATTCACTCGGACTGTTGATGGGAGTGTGACTTGGAAGCAGAAAGGCACAGGCAAATGAGACGTGCACTTGGAAGTTTGTTATTAGGAACATGCTCACTTGGAGAGTGGTCTCATAGCAAACTTCCTAATTCGTTTTAGATGCAGCCATGTTATTCCTCTCAGCAATATAAGGGTGACTATATTATAATTCACTCTCCTCGGAATGTACGTTCAAACCTATATTGAATGACTTGATTTGCTACGAGAAAAAATGTGTCTACTTACCAGCTGCCACCACAGAATTCAAATGGTGCAGCTAATATTATTTTGTAAGTGAATTTAATGAGATTTTGCAAATGCTTTTCCA

Ace file of the UniGene sp_v3.0_unigene5736

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	ser/arg-rich protein kinase 4 [Arabidopsis thaliana] gb\|AEE79027.1\| ser/arg-rich protein kinase 4 [Arabidopsis thaliana]	-	-	0.0	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	41%
Sma3	Srpk, putative	-	-	8.698e-12	-

Source	ECs	Term	Type	e value	Identity
Sma3	Dual-specificity kinase.	EC:2.7.12.1	-	3.95e-09	-

Source	Gene names
Sma3	AT4g35500; At2g17530; At3g44850; At4g35500; At4g35500/F8D20_10; At5g22840; CHLREDRAFT_105706; F28D10_40; F8D20.10; F8J2_200; GSVIVT00019335001; GSVIVT00021715001; GSVIVT00022115001; LOC_Os03g53720; MICPUCDRAFT_2123; MICPUN_67331; MtrDRAFT_AC139525g30v2; O

Source	GOs	Term	Type	Identity
Sma3	protein kinase activity	GO:0004672	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	kinase activity	GO:0016301	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G53030.1	SRPK4 ser/arg-rich protein kinase 4 chr3:19662412-19664362 FORWARD LENGTH=529	55%
RefSeq	Arabidopsis thaliana	NP_566977.1	ser/arg-rich protein kinase 4 [Arabidopsis thaliana]	55%
RefSeq	Populus trichocarpa	XP_002326404.1	predicted protein [Populus trichocarpa]	58%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LL66

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is longer than subject: 535 - 459

Fln protein: MATDTMVADPYMDSSSECSESEDEGTDDYRKGGYHAVRLGDSFKNGRYIVQRKLGWGHFSTVWLAWDLEEARFVALKVQKSAQHYGEAAMDEINILKEIAEGDPEDRKGVVKLLDHFKHVGPNGQHVCMVFEYLGDSLLSLIKYTRYRGLPLDIVRELCTPILVGLDYLHRELSIIHTDLKPENILLYSNIDPSKDPKNSKEPPLLSPIIDKPNLLLRSSSTKDTVNGAISRTQKNKLKRKAKRAANNCVSSVIAREKELDSANTDHGSISSGQTGVLNAEIILAGDSAAQHEADKKARYSAKIQSLKSIDLRCKIVDLGNACWIHKQFTPDIQTRQYRSPEVILGSKYSTPVDMWSFACIIFELATGDMLFDPQSAEGYDRDEDHLALMMELLGKIPRKIALGGSYSRELFDRHGDLKHIRRLRYWPLDRILVEKYKFSEKDADELAKFLCPLMDFAPEKRPTAAQCLLHPWLKCKMEDTRDRQRRESEGAKEVMEKAQVAMSNMAIKGTQAGFSRTAVPIRASNQQTSTMLK*

Protein Length: 536

Fln nts: ATGGGGCAATTAGGTAAGAGCGAGTCCGAGTTTGGTGAAAGGCCTTCATTAAACCCCCGACGCCCACGCCCACCATGGGGACCGCTACACGGCCATGATTGCGGGGATTGAAAGCTTCGCCTTGGTCTCAACGACTCACTGCGGTCCTGATACCACCTAACTGTACAAGCTGAAGTCCCCTCTCGCCCTCTCCTTGCTGCTGCAAAGATCTCGGGCGGTGCCTCTGTGGACAATTGAGTCAACAGGCTTCCAGGAGGTTAGGATAGTCTTATTTATCTCAAATCTAAAAGCAAAATCCAGAGAGGACACGAAATTCTGTTTTTGAACCGAGATTTGAGCTTTAAAATTAGATTTT_-_ATGGCCACCGATACCATGGTAGCGGATCCATACATGGACTCCTCAAGCGAATGCTCGGAATCTGAAGATGAAGGTACCGACGATTACAGGAAGGGAGGATACCATGCAGTTCGTTTGGGCGATTCGTTCAAGAATGGAAGATATATTGTGCAGCGAAAGCTCGGATGGGGTCATTTTTCTACTGTGTGGCTTGCTTGGGACCTCGAAGAAGCTAGATTTGTGGCTCTTAAGGTGCAGAAAAGTGCGCAACATTATGGAGAAGCAGCTATGGATGAGATCAACATCTTGAAGGAAATTGCAGAAGGAGATCCGGAAGATAGAAAAGGTGTTGTGAAATTGCTAGACCATTTCAAGCACGTTGGACCCAATGGGCAGCATGTTTGTATGGTTTTTGAGTATTTGGGTGACAGTTTGTTGTCATTGATAAAATATACTCGCTACCGAGGCCTTCCCTTGGACATTGTTCGGGAACTCTGCACCCCTATTTTAGTAGGATTGGATTATTTGCATCGAGAATTGTCAATTATTCACACTGATTTGAAACCTGAGAACATTCTTCTCTACTCTAATATCGATCCATCAAAAGATCCAAAGAACTCCAAGGAACCTCCCCTGCTTTCTCCTATTATAGATAAACCAAACCTACTTTTGAGGTCTTCCAGTACGAAAGACACAGTGAATGGAGCAATTTCAAGGACTCAGAAAAATAAATTAAAGAGAAAGGCTAAGAGGGCTGCAAACAACTGTGTGAGCAGTGTAATAGCTCGAGAAAAGGAATTAGATTCGGCTAATACAGACCACGGTAGTATAAGCAGTGGACAAACTGGTGTTTTAAACGCAGAAATCATTCTGGCTGGTGACTCAGCAGCTCAGCATGAGGCAGACAAAAAAGCTCGTTATTCTGCCAAGATTCAGAGCCTCAAATCAATAGATTTGAGATGTAAAATAGTAGACTTGGGAAATGCCTGCTGGATTCATAAGCAGTTTACACCTGATATTCAGACACGGCAATATCGATCCCCAGAGGTTATTTTGGGTTCAAAGTATTCTACACCAGTTGACATGTGGTCATTTGCTTGCATAATTTTTGAACTGGCTACAGGTGATATGTTATTTGATCCTCAGAGTGCAGAAGGTTATGACCGCGATGAGGACCACCTTGCCCTGATGATGGAGCTTCTTGGAAAAATACCTCGTAAGATAGCCTTAGGTGGGAGCTATTCACGGGAACTATTTGACAGGCATGGGGATTTAAAGCACATTAGACGGCTTCGGTATTGGCCCTTGGATCGTATACTAGTCGAGAAATATAAGTTCAGTGAGAAAGATGCTGATGAACTTGCCAAATTTTTATGCCCATTAATGGATTTTGCTCCAGAGAAGAGGCCTACAGCAGCGCAATGCCTCCTCCACCCATGGCTTAAATGCAAGATGGAAGACACAAGGGATAGACAAAGAAGGGAGAGTGAAGGTGCAAAGGAGGTCATGGAGAAAGCTCAAGTTGCTATGAGTAATATGGCTATCAAAGGAACTCAGGCAGGGTTCAGCAGAACAGCAGTGCCAATCAGGGCAAGCAATCAGCAAACAAGCACAATGCTCAAGTGAGTAGATTTAAGAAAAAGTCTGGTCTTCTAGAGTTATCGTCATAAGGATGCCTCCAGGTAGATACAGAACTGTACATTGCCATGCTGGTCGTCTTTCAAAGAATAACTCATTATATATTTCATGCGTGGGTATTAATTTAGGATGATTGATTTTATGGAGGCCTATTTCAAACAATCGTACCTACAAGTGAGTTTCAGTTACAAGATTGGTCTCGTTGTTTCAGAATCTAAAGAGCTGGAGTGTCCATCTCGACCCCCATCTTTGATTATATCTGTCTCAACTTATCAAGAACTTAAAAAGGTTCAAGGCCTTGGTGCCATGATTGCAACTCTTTGTCTGGAGTTCAATGTTTAGATGATGTCAAAATTGTTACTCCTTGTCCATAGGATGCTCTGATTTATGGTAAGATTTAGTACAGGATGGTTTCTATTGGTTATACAGCGGTTCACTCTGATTTGTAAAAAAGAGCATTCACTCGGACTGTTGATGGGAGTGTGACTTGGAAGCAGAAAGGCACAGGCAAATGAGACGTGCACTTGGAAGTTTGTTATTAGGAACATGCTCACTTGGAGAGTGGTCTCATAGCAAACTTCCTAATTCGTTTTAGATGCAGCCATGTTATTCCTCTCAGCAATATAAGGGTGACTATATTATAATTCACTCTCCTCGGAATGTACGTTCAAACCTATATTGAATGACTTGATTTGCTACGAGAAAAAATGTGTCTACTTACCAGCTGCCACCACAGAATTCAAATGGTGCAGCTAATATTATTTTGTAAGTGAATTTAATGAGATTTTGCAAATGCTTTTCCA

Fln Alignment:

Contig5736___KGGYHAVRLGDSFKNGRYIVQRKLGWGHFSTVWLAWDLEEARFVALKVQKSAQHYGEAAMDEINILKEIAEGDPEDRKGVVKLLDHFKHVGPNGQHVCMVFEYLGDSLLSLIKYTRYRGLPLDIVRELCTPILVGLDYLHRELSIIHTDLKPENILLYSN
B8LL66______________KDGHYMFALGDNL-TPRYKILNKIGEGTFGQVLECWDRETREFVAVKIVRGLQRYREAAMIEIDVLREVAKYDRSGTR-CVQMRNWFDY----RNHVCIVFEKLGPSLYDFLRKNNYHPFPIDIVRELGKQLLESVAYMH-HLHLIHTDLKPENILFASS

SNPs (tot: 9)

Position	A %	C %	G %	T %	Probability
1658	27	72			1.0
1885	11		88		0.923564
1886	88	11			0.923564
1888		88		11	0.923564
2271	28	71			1.0
2273			28	71	1.0
2746	85			14	0.993533
2762			66	33	0.997452
2763		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain