SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene5635

Length: 1505 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene5635 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	2-oxoisovalerate dehydrogenase E2 component (dihydrolipoyl transacylase) [Arabidopsis thaliana] ref\|NP_850527.1\| 2-oxoisovalerate dehydrogenase E2 component (dihydrolipoyl transacylase) [Arabidopsis thaliana] gb\|AAF63813.1\| branched chain alpha-keto acid	-	-	0.0	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	26%
Sma3	Dihydrolipoyllysine-residue acetyltransferase component of pyruvatedehydrogenase complex	-	-	5.027e-08	-

Source	Gene names
Sma3	AT3G06850; At3g06850; B1011A07.49; CHLREDRAFT_101057; F3E22.1; GSVIVT00033027001; LTA1; Os01g0314100; OsI_01602; OsJ_01492; PHYPADRAFT_136516; POPTRDRAFT_1089138; POPTRDRAFT_680137; RCOM_0681970; VITISV_019281; din3;

Source	GOs	Term	Type	Identity
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3		GO:0008415	Molecular Function	-
Sma3	dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity	GO:0043754	Molecular Function	-
Sma3	cofactor binding	GO:0048037	Molecular Function	-
Sma3	fatty-acyl-CoA biosynthetic process	GO:0046949	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Biotin/lipoyl attachment	IPR000089	-	-
Sma3	2-oxoacid dehydrogenase acyltransferase, catalytic domain	IPR001078	-	-
Sma3	2-oxo acid dehydrogenase, lipoyl-binding site	IPR003016	-	-
Sma3	E3 binding	IPR004167	-	-
Sma3	Lipoamide Acyltransferase	IPR015761	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G06850.1	BCE2, LTA1, DIN3 2-oxoacid dehydrogenases acyltransferase family protein chr3:2158212-2160465 REVERSE LENGTH=483	54%
RefSeq	Arabidopsis thaliana	NP_850527.1	2-oxoisovalerate dehydrogenase E2 component (dihydrolipoyl transacylase) [Arabidopsis thaliana]	54%
RefSeq	Populus trichocarpa	XP_002315510.1	predicted protein [Populus trichocarpa]	56%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LLY8

Fln msg: your sequence is shorter than subject: 412 - 566

Fln protein: MWEIQWKNFNDFVKSKSDKATIEITSRYKGKVSRVRFIPGCIIKVGETLLELSVDESHSNTTQLEGVQVVPTDVESGETVLTLSGSPKSRENRKSVLSTPAVRHIAKECGVNVEDIPGTGKDGRVMKEDVLKYVNDKESIWDEISPIAEATRSLEAEVMQSRSEVDSSLLKSTRQQILEDKSIPLRGFQRAMAKSMSIAAKVPHFHYLEEINVDSLAELKTAFQDATAGQGVKHTYLPFLIKSLSFALMEYPSMNSIFNEDAYEMICKGSHNIGVAMATTHGLVVPNIKNVQSLSILEITHEVQRLVQGASNNKLTSEDVSGGTITISNIGAIGGKFGSPLLNVPEVAIIAIGRIQKLPRFSDDETVYPALVMNVTIGADHRVIDGAAVANFCNQWKALIENPEHLLLHLR*

Protein Length: 413

Fln nts: AATGTGGGAGATTCAGTGGAAGAATTTCAACGACTTTGTGAAGTCCAAAAGTGATAAAGCAACTATAGAAATCACAAGTCGCTACAAGGGGAAAGTCTCTCGAGTGAGATTTATTCCAGGATGTATTATAAAGGTTGGAGAAACATTACTGGAATTAAGTGTAGATGAAAGTCATAGTAATACGACACAGTTAGAGGGAGTACAGGTGGTCCCTACAGATGTTGAGTCTGGGGAGACTGTTTTAACATTGAGCGGATCTCCAAAAAGCAGAGAAAACAGGAAAAGTGTATTGTCTACTCCAGCTGTTCGTCATATAGCAAAGGAATGTGGTGTTAATGTGGAAGACATTCCTGGTACTGGTAAAGATGGCAGGGTAATGAAAGAGGATGTTTTGAAATATGTAAATGATAAAGAGAGCATTTGGGATGAAATTTCTCCCATTGCTGAGGCCACTCGATCCTTAGAGGCTGAGGTTATGCAGTCAAGATCAGAAGTGGACTCATCCCTTCTAAAATCAACAAGACAACAAATCCTTGAAGATAAAAGCATTCCCCTCAGGGGATTCCAGCGTGCTATGGCGAAATCTATGAGCATTGCAGCCAAAGTTCCTCACTTCCATTATTTGGAAGAAATTAATGTTGATTCTCTTGCAGAGTTAAAAACAGCTTTTCAAGATGCAACAGCTGGGCAAGGTGTGAAACATACATACTTGCCGTTCCTGATAAAGTCATTATCTTTTGCCCTTATGGAGTATCCTTCAATGAACAGCATTTTCAATGAGGATGCATACGAAATGATTTGTAAAGGTTCACACAATATAGGAGTTGCCATGGCAACAACTCATGGCTTGGTTGTCCCCAACATAAAGAATGTTCAATCACTATCGATATTAGAGATTACACATGAGGTCCAACGTCTTGTTCAAGGAGCCTCAAACAACAAACTGACGTCAGAAGATGTTTCTGGGGGAACAATAACAATTAGCAACATTGGGGCTATTGGTGGTAAATTTGGATCTCCCCTCTTAAACGTACCTGAGGTTGCTATAATAGCAATTGGAAGAATTCAGAAGCTTCCTCGATTTTCAGACGATGAAACAGTTTATCCTGCTTTAGTTATGAATGTTACTATTGGGGCTGACCATAGAGTCATAGATGGTGCGGCTGTGGCAAACTTTTGCAACCAGTGGAAGGCTTTGATTGAAAATCCAGAGCATCTTCTCCTTCATTTGAGATAA___GCAGTTGGTTTGGCATCTAGACCAACTTTCTAAATTCCTTCTGCAGCAAACAAAGGCTGAGGTCAGAGCTCTGGACCAAGAGGCAAGATTGGCGAGTATGCTCTCGAGACTAGTCAGACTTGTGATAATTCGCTTGTTGCCTTTTTGGTTGTTATTATTGAACATTCATGCTTATGTTTATCAGTATTTTTTCGGAGTTTAACTATTTTTTAGCGTTGTCTAACGTCATTTTGTAATCTTGATTTACTGTTAATAAAATTTTATGTCC

Fln Alignment:

Contig5635___KSDKATIEITSRYKGKVSRVRFIPGCI-IKVGETLLELSVDE----------------SHSNTTQLEGVQVVPTDVESGETVLTLSGSPKSRENRKS---VLSTPAVRHIAKECGVNVEDIPGTGKDGRVMKEDVLKYVNDKESIWDEISPIAEATRSLEAEVMQSRSEVDSSLLKSTRQQILEDKSIPLRGFQRAMAKSMSIAAK-VPHFHYLEEINVDSLAELKT---AFQDATAGQGVKHTYLPFLIKSLSFALMEYPSMNSIFNEDAYEMICKGSHNIGVAMATTHGLVVPNIKNVQSLSILEITHEVQRLVQGASNNKLTSEDVSGGTITISNIGA-IGGKFGSPLLNVPEVAIIAIGRIQK--LPRFSDDETVYPALVMNVTIGADHRVIDGAAVANFCNQWKALIENPEHLLL
B8LLY8______________ETDKAIVDMESMEDGYLAKIVHGDGAKEIKIGEVIAIMVEDEDDIAKFKDYTPSGQGAANEKAPSKETTPPPPPPKEDTPSPVTIPKTEKSTASPQSEDRIFASPIARKMAEDHKVPISSIKGTGPNGRIVKADIEDYL---ASVSKATPPSTPPTKTLEYT------------------------DIPLSQIRKVTASRLLLSKQTIPHYYLTVDTCVDKLMVLRNQLNALQEASNGKRISVN--DFVIKAAASALRKVPQCNSSWTNEYIRQY--HNINISVAVQTDKGLFVPVVKDADKKGLSAIGEDVKVLAQKAKENTLKPADYEGGTFTVSNLGGPFGIKQFCAIINPPQSAILAVGSAEKRVIPGALQDQFDVGSF-MSVTLSCDHRVIDGAIGAEYLKAFKGYIEDPLTMLL

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
536		25		75	0.996473
689		25		75	0.996473
717		33	66		0.997452
718		33		66	0.997452
739		66		33	0.997452
991		33	66		0.997452
992	66	33			0.997452
993		33		66	0.997452
994	33			66	0.997452
1338	16			83	0.994512
1339	16			16	0.988813
1341	16			83	0.994512
1386		33		66	0.997452
1387	33	66			0.997452
1389		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain