SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene4404

Length: 1158 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene4404

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Dihydroflavonol 4-reductase n=4 Tax=Lotus RepID=Q403G8_LOTJA	-	-	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	53%
Sma3	Dihydroflavonol 4-reductase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Dihydrokaempferol 4-reductase.	EC:1.1.1.219	-	4.133e-39	-

Source	KEGGs	Term	e value	Identity
Sma3	Flavonoid biosynthesis	00941	4.133e-39	%
Sma3	Biosynthesis of phenylpropanoids	01061	4.133e-39	%
Sma3	Metabolic pathways	01100	4.133e-39	%
Sma3	Biosynthesis of secondary metabolites	01110	4.133e-39	%

Source	Gene names
Sma3	A; A1* (second gene)/ (Teosinte); AN6; ANR; AT4g35420; ApDFR; At1g51410; At4g27250; At5g42800; CAD; CAD1-7; CCRL4; CCRL5; CtDFR; DFR; DFR-A; DFR-B; DFR-C; DFR1; DFR2; DFR4; DFRA; Dfr1; Dfr2; F23E12.20; F5D21.12; GSVIVT00000921001; GSVIVT00005344001; GSVIV

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	extrinsic to endoplasmic reticulum membrane	GO:0042406	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	binding	GO:0005488	Molecular Function	-
Sma3	transcription repressor activity	GO:0016564	Molecular Function	-
Sma3	dihydrokaempferol 4-reductase activity	GO:0045552	Molecular Function	-
Sma3	coenzyme binding	GO:0050662	Molecular Function	-
Sma3	regulation of nitrogen utilization	GO:0006808	Biological Process	-
Sma3	flavonoid biosynthetic process	GO:0009813	Biological Process	-
Sma3	cellular metabolic process	GO:0044237	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	NAD-dependent epimerase/dehydratase	IPR001509	-	-
Sma3	NmrA-like	IPR008030	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT5G42800.1	DFR, TT3, M318 dihydroflavonol 4-reductase chr5:17164296-17165864 REVERSE LENGTH=382	0.0	54%
RefSeq	Arabidopsis thaliana	NP_199094.1	dihydroflavonol-4-reductase [Arabidopsis thaliana]	0.0	54%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NNA9

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 201 aas, putative chimeric sequence, atg_distance in limit (1-15): atg_distance = 13, W2: There is no M at the beginning, your sequence is shorter than subject: 139 - 351

Fln protein: NGDEACEENKRVVCVTGANGYIGSWLVMRLLEHGYYVHGTVRDPEDPGKVGHLLGLPGASEKLKLFKADLNNEMAFDDAVSGCQGVFHVAKPVNLDSNALQGEVVGPAVRGTVNLLRACERSGTVKRVIHTSSVSAAP*

Protein Length: 140

Fln nts: GGA_-_ATGGAGATGAAGCATGCGAAGAGAACAAGCGAGTGGTTTGTGTAACAGGGGCAAATGGGTACATCGGCTCTTGGCTGGTCATGAGATTACTGGAACATGGCTATTATGTTCATGGAACTGTCAGGGACCCAGAAGACCCAGGGAAGGTTGGGCATTTGCTGGGGCTTCCAGGGGCAAGTGAGAAGCTAAAGCTGTTCAAGGCAGATCTCAACAACGAAATGGCCTTTGATGATGCTGTGAGCGGTTGTCAAGGAGTTTTCCATGTTGCCAAGCCTGTTAATCTGGACTCAAACGCTCTTCAGGGGGAGGTTGTTGGTCCTGCGGTGAGGGGAACAGTAAATCTGCTTCGGGCCTGCGAACGATCGGGCACTGTGAAACGAGTGATACATACCTCGTCCGTTTCAGCAGCCCCCTGATACTGTGCTGGATGAATCTCATTGGACATCGGTCGAGTATTGCAGAAAGACAAAGATGGTCGGATGGATGTACTACATCGCCAACACTTATGCAGAAGAGGGAGCCCATAAGTTCGGATCAGAGAACAAAATGGACGTTATCAGCATCCTACCAGCAATCACTGTGGGGGATTTTCTCACTCTTCAGATCCCAGGCAGCGTGGCTACTCTCATGGCGTTGCCCAAAGGAGATGCAGACTTTTACTCTTACACCCCACGTGTCCCTTACGTACACATAGAGGACGTTGTGAGGGCTTTCATCTTCTTGTATGAACATCCAGACTCCAAAGGGAGGTACATCTGCTCTCTCCAGGACGTAGCCTTGGAAGAACTTGCTCAAGTCTTTCAGAACCGACATCAACTTAATGTACCTGCAAAGTTTGATAATCTTCGTAGTGATTACATATATCCTTGTGTGGACTCTGGAAAGCTGAAAAAGCTTGGATTTGAGTACAAATATTCTTTAGAGGATATCGTTGATGATGCAGTCAAATCTTATAAACGGAGAGGACTCTTTTAAATGGCACATACGCCTGGCATGATCATAATGTTATTTGCTTGGTAGTGTGACAGTATGCTTCAATAGTTTCACTGTTACTCGTAAAGTTTCATTTATATTTTCAGAAAATAAATCTCATTTTACGTTTTGGCATCTGCTGATCAATCTGTGAATGCCAACGGTGCTTCTTCGGTTGAAGTT

Fln Alignment:

Contig4404___NKRVVCVTGANGYIGSWLVMRLLEHGYYVHGTVRDPEDPGKVGHLLGLPGASEKLKLFKADLNNEMAFDDAVSGCQGVFHVAKPVNLDSNALQGEVVGPAVRGTVNLLRACERSGTVKRVIHTSS
A9NNA9______________HRGTVCVTGAAGFIGSWLIMRLLERGYTVRATVRDTGKPLKTKHLLDLPGANERLTLWKADLDDERSFDAAVDGCEGVFHVATPMDFESEDPENDIIKPTVNGVLNVMKSCAKAKSVKRVVFTSS

SNPs (tot: 1)

Position	A %	C %	G %	T %	Probability
771		20	80		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain