SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene3527

Length: 2044 nt

UniGene Fasta

>sp_v3.0_unigene3527
TCAGGAAGGATGCCCGATGTCAGACTGGTTCAATATTTTAGTTCTTCATCAAAACAGGGTCAAAACCAATCCAAAAAATGCCATTAATGAGCAGTTCCTTCCACGTTTCTTAGATCTTGTAATTTGGGGGCATGAACACGAATGCTTGGTTGACCCACAGGAAGTTCCTGGAATGGGTTTTCATTTAACACAGCCAGGCTCTTCAGTTGCAACATCCCTGATTGATGGTGAAGCAAAGCCCAAGCATGTGCTTCTTCTGGATATAAAGGGGAGCCAATATTCGACCGACGAAAATCCCTTTGCAGTCAGTGAGACCTTTCGAATATCAAGAGGTCGTATTGAAGGATGAACCTGATTTAGATCCAAATGATCAAGATGCTGTTCTAGAATTTCTAGATAAAGTGGTAAAAGATATGATTATACTGGCTGAAAGAAAGAAAGTTAGTCGTTCGGAACTCAAGATTCCTTTAGTTCGAATCAAGGTAGACTATACAGGCTTCACAACTATAAATCCTCAAAGATTTGGTCAAAAATTTGTTGGAAAGGTTGCAAATCCACATGACATTCTTCTCTTTTCAAAGGCCACAAGAAGGCAGCAATCCATGGACGAAAAGATTCTAGATGCTGAACGCCTGCGACCAGAGGAACTTAACCAGCAAAACATTGAGGCTCTTGTTGCAGAAAGTAATCTGAAGATGGAGATCCTTCCGGTTAATGATCTAGATGTTGCACTGCATGAATTTGTTAACAAGGACGATAAATTGGCTTTCCACTCTTGTTTGCAGCATAATCTTGAGGAGACACGGAATAAACTTGTGGAAGAAGTAGATGTATTGCAAATTAAAGAAGAGGAGGATTTAGTGCACAAAGTGGGCCAATGCATGCAGGAACGTGAAAAAGAGAGAGCATTACGTCCAAAGACAGGTTTGTCAGTTGGGTCTAGCTCTCAGCTCTCACAGGGCATAAAAAATAAAAACTCATTACAACTAGCCAGCCAGAGTGCGTTCAGTGATGAAGAAGATGAACCTGTGAAAGTCACTAAGTCAAAAGTTGTGAGTCGGGGCCGTGGAACAAAATCATCACAAGCTTCTTCTAGAGTAACTGATAATATTCCAGAAAGTACTCAAAGAGGAAGAGGTACCAAGGGTAGAGGAAGAGGAAGAGATTCACGTGGTCTTAAGCAAACCACTTTGGATGTTGCTATGGGAAGCCGTCATTCAGAGAGAACTGCATCAGCTGCAGCATCTGTGGCGGTACGGGGTGTTGTAGTTGATGAAGAAGATGTGAATTCACTTTCAAGTGATGAAATTGAAAAAGAAGAAATAGATGAACCAGACAATGGCTTGGAGCCTGATGAAACCCCTAAAAGTGCAAAAGGACGCAAGAGACCTGCCCCACAAGGGAAAGGTAGAGGACGAGCTACGGGCTCTACTGGGAAACGGGGAAGGAAATCTGGAAATACTGTAGTCAGACAAATGTTCATTGATGATGATGAAGATGAGACTTATGATGAACCAAGAACATCCAAAGTTTCCAGTCCTAAGGTTAATCGCAATTGGGGATCTCTAAGGAGATAGCAGTTTTCAATATCATCCAGATATTCTGCTGTCATCATTCATATATACAAATTGGAGGACCTCACATGATAGAAAACCCTCAATTTTGCAGATTTGCAGGTTTCATTATACTCTTGTCAACAGCTATGCATACTTGTGAATACTATTTATGTACATCAGATCCTGTAATTATATCAATAAAAAATCCATACAACATGATTTTCTAAAGTGAGTAGCTTCTTCTTCACATCCATAAGATGAATGGCATTCTACATGCAGTTGCTTCGTTGGAAACACTAAATATACTAGTGAGAAGCAAAAATGAGTGGCCATGACTGCTTGTGCATGACAGCACTTCATTTGTAATTTATATTTAGTAGAGGACATCAATGCCACGGAAGCATTGCTGCCTTGATGGTATCCAATGCATGATATTTTTCAACAAATAATTACATGTTTCTTTCTGTTCATTCTTTCGGTCATTTCGC

Ace file of the UniGene sp_v3.0_unigene3527

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	double-strand break repair protein MRE11 [Arabidopsis thaliana] sp\|Q9XGM2.1\|MRE11_ARATH RecName: Full=Double-strand break repair protein MRE11 emb\|CAB50793.1\| Mre11 protein [Arabidopsis thaliana] dbj\|BAB10749.1\| DNA repair and meiosis protein Mre11 [Arabi	-	-	0.0	50%
FL-Next	sp=Double-strand break repair protein MRE11; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	76%
Sma3	Double-strand break repair protein MRE11	-	-	7.997e-09	-

Source	Gene names
Sma3	At5g54260; GSVIVT00011139001; GSVIVT00027588001; GSVIVT00027589001; H0410G08.11; MDK4.8; MICPUCDRAFT_70239; MRE11; Mre11B; OJ1120_C08.16; OSJNBa0043L09.28; Os04g0635900; OsI_17582; OsI_28027; OsJ_16317; OsJ_26244; P0583B06.24; PHYPADRAFT_30474; POPTRDRAFT

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	endonuclease activity	GO:0004519	Molecular Function	-
Sma3	exonuclease activity	GO:0004527	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	manganese ion binding	GO:0030145	Molecular Function	-
Sma3	double-strand break repair	GO:0006302	Biological Process	-
Sma3	meiosis	GO:0007126	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	DNA repair exonuclease	IPR003701	-	-
Sma3	Metallophosphoesterase domain	IPR004843	-	-
Sma3	Mre11, DNA-binding	IPR007281	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G54260.1	MRE11, ATMRE11 DNA repair and meiosis protein (Mre11) chr5:22032702-22037749 FORWARD LENGTH=720	53%
RefSeq	Arabidopsis thaliana	NP_200237.1	double-strand break repair protein MRE11 [Arabidopsis thaliana]	53%
RefSeq	Populus trichocarpa	XP_002330391.1	predicted protein [Populus trichocarpa]	50%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: Q9XGM2

Fln msg: Overlapping hits, possible frame ERROR between 319 and 267, your sequence is shorter than subject: 526 - 720

Fln protein: QEGCPMSDWFNILVLHQNRVKTNPKNAINEQFLPRFLDLVIWGHEHECLVDPQEVPGMGFHLTQPGSSVATSLIDGEAKPKHVLLLDIxxxxxxxxxxxxxxxxxxxEYQEVVLKDEPDLDPNDQDAVLEFLDKVVKDMIILAERKKVSRSELKIPLVRIKVDYTGFTTINPQRFGQKFVGKVANPHDILLFSKATRRQQSMDEKILDAERLRPEELNQQNIEALVAESNLKMEILPVNDLDVALHEFVNKDDKLAFHSCLQHNLEETRNKLVEEVDVLQIKEEEDLVHKVGQCMQEREKERALRPKTGLSVGSSSQLSQGIKNKNSLQLASQSAFSDEEDEPVKVTKSKVVSRGRGTKSSQASSRVTDNIPESTQRGRGTKGRGRGRDSRGLKQTTLDVAMGSRHSERTASAAASVAVRGVVVDEEDVNSLSSDEIEKEEIDEPDNGLEPDETPKSAKGRKRPAPQGKGRGRATGSTGKRGRKSGNTVVRQMFIDDDEDETYDEPRTSKVSSPKVNRNWGSLRR*

Protein Length: 527

Fln nts: TCAGGAAGGATGCCCGATGTCAGACTGGTTCAATATTTTAGTTCTTCATCAAAACAGGGTCAAAACCAATCCAAAAAATGCCATTAATGAGCAGTTCCTTCCACGTTTCTTAGATCTTGTAATTTGGGGGCATGAACACGAATGCTTGGTTGACCCACAGGAAGTTCCTGGAATGGGTTTTCATTTAACACAGCCAGGCTCTTCAGTTGCAACATCCCTGATTGATGGTGAAGCAAAGCCCAAGCATGTGCTTCTTCTGGATATAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGAATATCAAGAGGTCGTATTGAAGGATGAACCTGATTTAGATCCAAATGATCAAGATGCTGTTCTAGAATTTCTAGATAAAGTGGTAAAAGATATGATTATACTGGCTGAAAGAAAGAAAGTTAGTCGTTCGGAACTCAAGATTCCTTTAGTTCGAATCAAGGTAGACTATACAGGCTTCACAACTATAAATCCTCAAAGATTTGGTCAAAAATTTGTTGGAAAGGTTGCAAATCCACATGACATTCTTCTCTTTTCAAAGGCCACAAGAAGGCAGCAATCCATGGACGAAAAGATTCTAGATGCTGAACGCCTGCGACCAGAGGAACTTAACCAGCAAAACATTGAGGCTCTTGTTGCAGAAAGTAATCTGAAGATGGAGATCCTTCCGGTTAATGATCTAGATGTTGCACTGCATGAATTTGTTAACAAGGACGATAAATTGGCTTTCCACTCTTGTTTGCAGCATAATCTTGAGGAGACACGGAATAAACTTGTGGAAGAAGTAGATGTATTGCAAATTAAAGAAGAGGAGGATTTAGTGCACAAAGTGGGCCAATGCATGCAGGAACGTGAAAAAGAGAGAGCATTACGTCCAAAGACAGGTTTGTCAGTTGGGTCTAGCTCTCAGCTCTCACAGGGCATAAAAAATAAAAACTCATTACAACTAGCCAGCCAGAGTGCGTTCAGTGATGAAGAAGATGAACCTGTGAAAGTCACTAAGTCAAAAGTTGTGAGTCGGGGCCGTGGAACAAAATCATCACAAGCTTCTTCTAGAGTAACTGATAATATTCCAGAAAGTACTCAAAGAGGAAGAGGTACCAAGGGTAGAGGAAGAGGAAGAGATTCACGTGGTCTTAAGCAAACCACTTTGGATGTTGCTATGGGAAGCCGTCATTCAGAGAGAACTGCATCAGCTGCAGCATCTGTGGCGGTACGGGGTGTTGTAGTTGATGAAGAAGATGTGAATTCACTTTCAAGTGATGAAATTGAAAAAGAAGAAATAGATGAACCAGACAATGGCTTGGAGCCTGATGAAACCCCTAAAAGTGCAAAAGGACGCAAGAGACCTGCCCCACAAGGGAAAGGTAGAGGACGAGCTACGGGCTCTACTGGGAAACGGGGAAGGAAATCTGGAAATACTGTAGTCAGACAAATGTTCATTGATGATGATGAAGATGAGACTTATGATGAACCAAGAACATCCAAAGTTTCCAGTCCTAAGGTTAATCGCAATTGGGGATCTCTAAGGAGATAGCAGTTTTCAATATCATCCAGATATTCTGCTGTCATCATTCATATATACAAATTGGAGGACCTCACATGATAGAAAACCCTCAATTTTGCAGATTTGCAGGTTTCATTATACTCTTGTCAACAGCTATGCATACTTGTGAATACTATTTATGTACATCAGATCCTGTAATTATATCAATAAAAAATCCATACAACATGATTTTCTAAAGTGAGTAGCTTCTTCTTCACATCCATAAGATGAATGGCATTCTACATGCAGTTGCTTCGTTGGAAACACTAAATATACTAGTGAGAAGCAAAAATGAGTGGCCATGACTGCTTGTGCATGACAGCACTTCATTTGTAATTTATATTTAGTAGAGGACATCAATGCCACGGAAGCATTGCTGCCTTGATGGTATCCAATGCATGATATTTTTCAACAAATAATTACATGTTTCTTTCTGTTCATTCTTTCGGTCATTTCGC

Fln Alignment:

Contig3527___QEGCPMSDWFNILVLHQNRVKTNPKNAINEQFLPRFLDLVIWGHEHECLVDPQEVPGMGFHLTQPGSSVATSLIDGEAKPKHVLLLDIxxxxxxxxxxxxxxxxxxEYQEVVLKDEPDLDPNDQDAVLEFLDKVVKDMIILAERKKVSRSELKIPLVRIKVDYTGFTTINPQRFGQKFVGKVANPHDILLFSKATRRQQSMDEKILDAERLRPEELNQQNIEALVAESNLKMEILPVNDLDVALHEFVNKDDKLAFHSCLQHNLEETRNKLVEEVDVLQIKEEEDLVHKVGQCMQEREKERALRPKTXXXXXXXXXXXXXIKNKNSLQLASQSAFSDEEDEPVKVTKSKVVSRGRGTKSSQASSRVTDNIPESTQXXXXXXXXXXXXXXXXLKQTTLDVAMGSRHSERT-XXXXXXXXXXXXXXDEEDVNSLXXXXXXXXXXXXPDNGLEPDETPKSAKGRKRPAPQGKGRGRATGSTGKRGRK--SGNTVVRQMFIDDDEDETYDEPRTSKV--SSPKVNRNWGSLRR
Q9XGM2______________QEGCDVSDWFNILVLHQNRVKSNPKNAISEHFLPRFLDFIVWGHEHECLIDPQEVSGMGFHITQPGSSVATSLIDGESKPKHVLLLEIxxxxxxxxxxxxxxxxxxEYTEIVLKDESDIDPNDQNSILEHLDKVVRNLIEKASKKAVNRSEIKLPLVRIKVDYSGFMTINPQRFGQKYVGKVANPQDILIFSKASKKGRS-EANIDDSERLRPEELNQQNIEALVAESNLKMEILPVNDLDVALHNFVNKDDKLAFYSCVQYNLQETRGKLAKDSDAKKF-EEDDLILKVGECLEERLKDRSTRP-TGSSQFLSTGLTSENLTKGSSGIANAS-FSDDED-TTQMSGLAPPTRGRRGSSTANTTRGRAKAPTRGR--------GRGKASSAMKQTTLDSSLGFRQSQRSASAAASAAFKSASTIGEDDVDSPSSEEVEPEDFNKPDSSSEDDESTK-GKGRKRPATTKRGRGRGSG-TSKRGRKNESSSSLNRLLSSKDDDEDEDDEDREKKLNKSQPRVTRNYGALRR

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
855	20		80		0.995493
1499			66	33	0.997452
1503	66		33		0.997452
1511	20		80		0.995493
1515	80			20	0.995493
1636	15		84		0.999986
1656		75		25	1.0
1676		66		16	0.999991
1787	14		85		0.993533
1823			85	14	0.993533
1824		85		14	0.993533
1878	80		20		0.995493
1990			66	33	0.997452

SSRs (tot: 2)

Start position	End position	Sequence	Length
429	441	GAAA GAAA GAAA G	13
1484	1495	TGA TGA TGA TGA	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain