SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene3021

Length: 1842 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene3021

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Metal transporter Nramp3 n=3 Tax=Poaceae RepID=NRAM3_ORYSJ	-	-	0.0	68%
FL-Next	sp=Metal transporter Nramp3; Oryza sativa subsp. japonica (Rice).	-	-	0.0	68%
Sma3	Nramp transporter	-	-	1.228e-22	-

Source	Gene names
Sma3	At1g15960; At1g47240; At1g80830; At2g23150; At4g18790; At5g67330; B1153E06.4; CHLREDRAFT_196299; CsNramp1; DMT1; F21P24.21; F23A5.18; F28A21.200; F8G22.4; GSVIVT00011628001; GSVIVT00014922001; GSVIVT00015427001; GSVIVT00015429001; GSVIVT00015430001; GSVIV

Source	GOs	Term	Type	Identity
Sma3	vacuolar membrane	GO:0005774	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	transporter activity	GO:0005215	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	transport	GO:0006810	Biological Process	-
Sma3	iron ion transport	GO:0006826	Biological Process	-
Sma3	cellular iron ion homeostasis	GO:0006879	Biological Process	-
Sma3	response to nematode	GO:0009624	Biological Process	-
Sma3	response to iron ion	GO:0010039	Biological Process	-
Sma3	response to zinc ion	GO:0010043	Biological Process	-
Sma3	positive regulation of oxygen and reactive oxygen species metabolic process	GO:0010670	Biological Process	-
Sma3	defense response to bacterium	GO:0042742	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	RNA recognition motif domain	IPR000504	-	-
Sma3	Natural resistance-associated macrophage protein	IPR001046	-	-
Sma3	Nuclear transport factor 2	IPR002075	-	-
Sma3	K Homology domain	IPR004087	-	-
Sma3	K Homology domain, type 1	IPR004088	-	-
Sma3	Nucleotide-binding, alpha-beta plait	IPR012677	-	-
Sma3		IPR018111	-	-
Sma3	Nuclear transport factor 2, Eukaryote	IPR018222	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G15960.1	NRAMP6, ATNRAMP6 NRAMP metal ion transporter 6 chr1:5482202-5485066 REVERSE LENGTH=527	68%
RefSeq	Arabidopsis thaliana	NP_173048.3	metal transporter Nramp6 [Arabidopsis thaliana]	68%
RefSeq	Populus trichocarpa	XP_002332060.1	nramp transporter [Populus trichocarpa]	70%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q653V6

Fln msg: Separated hits, possible frame ERROR between 946 and 1008, and possible frame ERROR between 1304 and 1312,

Fln protein: MIHSEAEDQASKANRYTPINAERGGEGDEPFLHSDGNLAVQQDLKITQVSVPEKHGLRKLFAYLGPGFLVAIAYLDPGNFESDLQAGARYKYELLWVLLLATIAGAVIQSLASKLGVVTGKHLAEHCRAEYPTKTNYILWLLAELAIVASDIPEVIGTAYALNMLFRLPLWLGVILTGLSTLGFLVLQQYGVRKLELFISLLVLTIASCFFVELKYAKPSSTEVMKGLFIPRLGGHGATGIAISLLGAMAMPHNLFLHSALVLSRQTPRTTQGIKDGCRYNVIECSLALLxxxxxxxxxxxxxxxxxxxxxxLSDEDKANCSSLDLNRAPFLLKHVLGKWSSTLFGIALLASGQSSTITGTYAGQYVMQGFLHLRFAPWIRNMLTRCVAIIPSLVAALIGGSSGAGNLIVxxxRMILSFELPFALLPLLKFTSNNVKMGPHVNSKKVAVLTWVIGTCVILINMFYLMSGLIHWFTNTALPTTRVVFLGIIGVAALMAYIGFTLYLALRTDRTETYNSPERVEIIMNELNENVLDNLDEGGRLVMLPNDNIVDDFPN*

Protein Length: 558

Fln nts: GATATCGGCCTGGTAGTGCTCATCATCTTTATGCACAGACGCGGAGGGTTTTGTATTATAGTTACTGATAGCT_-_ATGATACACTCAGAAGCAGAAGACCAGGCCTCGAAAGCTAACAGGTACACTCCCATAAATGCGGAAAGAGGAGGAGAAGGAGACGAACCCTTTCTTCACTCTGATGGAAATCTTGCAGTTCAACAAGACCTGAAGATCACACAAGTCAGCGTTCCTGAGAAACATGGCTTGAGGAAGCTGTTTGCTTATCTTGGCCCTGGTTTTTTGGTCGCAATTGCTTATTTGGACCCAGGAAACTTTGAAAGTGATCTTCAAGCAGGTGCACGCTATAAATATGAGCTACTTTGGGTACTTTTACTGGCAACTATTGCTGGTGCTGTGATCCAGTCTCTAGCTTCAAAATTAGGCGTTGTCACAGGTAAACATTTGGCAGAGCATTGTCGAGCAGAGTATCCAACCAAAACTAACTACATACTCTGGTTACTTGCTGAACTGGCTATTGTTGCTAGTGATATCCCAGAAGTTATTGGGACAGCCTATGCCTTAAATATGCTGTTTCGTCTTCCACTATGGTTAGGAGTTATCCTCACAGGATTGAGTACTCTGGGCTTTCTTGTTCTGCAGCAATATGGGGTGCGGAAATTGGAATTATTTATCTCCCTTCTGGTCCTCACCATAGCTAGCTGTTTCTTTGTAGAGTTAAAATATGCAAAACCATCCAGTACAGAAGTTATGAAGGGACTGTTCATTCCAAGATTGGGTGGTCATGGAGCAACTGGGATAGCGATTTCTCTTCTTGGTGCAATGGCTATGCCGCATAATCTGTTTTTACATTCAGCTCTGGTGTTGTCAAGACAAACTCCCAGAACAACTCAAGGAATCAAGGATGGTTGCAGATACAATGTTATTGAGTGCAGCTTGGCTCTTTTGTTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCCTTTCAGATGAAGATAAAGCAAATTGCAGTAGTCTTGATTTGAACCGTGCACCCTTTCTCCTAAAACATGTTCTTGGGAAATGGAGTTCAACACTGTTTGGCATTGCTCTGCTAGCTTCTGGTCAAAGTTCGACAATAACAGGGACCTATGCTGGACAGTATGTGATGCAGGGATTTTTACATTTACGGTTTGCACCATGGATTAGGAATATGCTTACAAGATGCGTTGCAATAATCCCTAGCTTGGTTGCTGCTCTTATTGGGGGCTCTAGTGGTGCTGGAAATCTCATTGTCxxxxxxxxxCGGATGATTCTTTCTTTTGAGCTTCCTTTTGCTCTTCTGCCACTTTTGAAATTTACAAGCAACAATGTAAAGATGGGTCCTCATGTTAATTCAAAGAAGGTTGCTGTATTGACATGGGTCATAGGAACTTGCGTTATATTAATAAACATGTTCTATCTAATGAGTGGACTTATTCACTGGTTCACAAATACTGCACTGCCGACAACACGCGTAGTATTTCTTGGGATAATTGGTGTTGCTGCCTTGATGGCTTATATCGGTTTCACATTGTATTTAGCACTGAGAACAGACAGGACAGAGACTTATAACTCGCCTGAAAGAGTTGAAATTATAATGAATGAGCTGAATGAAAATGTTCTAGATAACCTTGACGAGGGAGGGCGTTTGGTAATGCTTCCTAATGATAACATTGTGGACGACTTTCCAAATTAG___TAGCTAATTTTGGTTTCAACGTGTTTCCACTCCCACACTTTATCGCCTTTTTCATTAATTATCAGCCCTTTTCTGGTCTAAAAATTTATATTTGTTCCCATC

Fln Alignment:

Contig3021___LKITQVSVPEKHGLRKLFAYLGPGFLVAIAYLDPGNFESDLQAGARYKYELLWVLLLATIAGAVIQSLASKLGVVTGKHLAEHCRAEYPTKTNYILWLLAELAIVASDIPEVIGTAYALNMLFRLPLWLGVILTGLSTLGFLVLQQYGVRKLELFISLLVLTIASCFFVELKYAKPSSTEVMKGLFIPRLGGHGATGIAISLLGAMAMPHNLFLHSALVLSRQTPRTTQGIKDGCRYNVIECSLALLLxxxxxxxxxxxxxxxxxxxxxLSDEDKANCSSLDLNRAPFLLKHVLGKWSSTLFGIALLASGQSSTITGTYAGQYVMQGFLHLRFAPWIRNMLTRCVAIIPSLVAALIGGSSGAGNLIVMxxxMILSFELPFALLPLLKFTSNNVKMGPHVNSKKVAVLTWVIGTCVILINMFYLMSGLIHWFTNTALPTTRVVFLGIIGVAALMAYIGFTLYLALRTDRTET
Q653V6______________IDVDQIVIPEKNSWKNLFSYIGPGFLVSIAYIDPGNFETDLQAGAQYKYELLWIILIASCAALIIQSLAARLGVVTGKHLAEHCRAEYPKATNFILWILAELAVVACDIPEVIGTAFALNMLFKIPVWCGVLITGLSTLMLLLLQQYGVRKLEFLIAILVSLIATCFLVELGYSKPNSSEVVRGLFVPELKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKVPRSVHGIKEACRFYMIESAFALTIxxxxxxxxxxxxxxxxxxxxxLSPEDQMNCSDLDLNKASFLLKNVLGNWSSKLFAVALLASGQSSTITGTYAGQYVMQGFLDLRMTPWIRNLLTRSLAILPSLIVSIIGGSSAAGQLIIIxxxMILSFELPFALVPLLKFTSSRTKMGQHTNSKAISVITWGIGSFIVVINTYFLITSFVKLLLHNGLSTVSQVFSGIFGFLGMLIYMAAILYLVFRKNRKAT

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
671	83			16	0.994512
673	16	83			0.994512
675		83	16		0.994512
676		83		16	0.994512
677		83		16	0.994512
680		83		16	0.994512
690		83		16	0.994512
712	83		16		0.994512
716	16			83	0.994512
736		85		14	0.993533
740	85		14		0.993533
748			14	85	0.993533
751	14		85		0.993533
758		87		12	0.992554
760	12		87		0.992554
773		12		87	0.992554
775	12		87		0.992554
776	12		87		0.992554
777	12		87		0.992554
778	12			87	0.992554
847	75		25		0.996473
1236		33		66	0.997452
1321	75	25			0.996473
1322			25	75	0.996473
1325			25	75	0.996473
1327			25	75	0.996473
1328	25	75			0.996473
1329		25		75	0.996473
1330		25		75	0.996473
1342		25		75	0.996473
1813		66		33	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain