SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene2998

Length: 2704 nt

UniGene Fasta

>sp_v3.0_unigene2998
GACATATCATCTGATTGAGTTCAATATCTCTGCAACTGGTGTTGCATTTAGGAAGATGGCCTTGCCTTGCTCTTCATTGTCATCCCACATTCATACTGGAGCTCACCACCATGGTAACACACAACGCATTCCACATTTCCACAGCTCGTTTAATGCAGGAACCAGTGCTGGCAAACGGAGAAATTTGTACCAACGATGGGGTACTAAAGGTTCAAGAAAGATTGTTGCTTGTGTTGGGGAAGATCCTACAGCACGGGTGAAACCAGAATTTTCTCCAAGTTTTTGGGAGGACCATATCGAATCCCTAATGCCATCGTACAAGGTTGCACCATCGGACGAGAAGCGTATCGAGACATTAATATCCGAGATTAAGAATATGTTTCGATCTATGGGCTATGGGGAAACTAATCCCTCTGCATATGACACTGCTTGGGTAGCAAAGATTCCAGCACTTGATGGCTCTGACTACCCTCACTTTCCTCAAACACTCCAATGGCTTCTTCAAAATCAGTTGAAAGATGGCTCTTGGGGAGAAGAGAACTACTTCTCAACATATGACAGACTACTCGCTACCCTCGCGTGTATTATTACTCTCACTGTATGGCGCACTGGCCAGACACAAGTGCGGCAAGGTATTGAATTCTTCAAAAAGCACGCTGGAACGATGGAGGATGAGGCGGACAATCACCAGCCAATTGGATTTGAATTTGTCTTTCCTGCAATGATAAATGAAGCTAAAAGCTTATGCTTGGATTTGCCTTATGATATGCCATTCATCAAACAAATCATTGAGAAGCGGGAGGCTAAACTTAAAAGGATTCCCACTGATACTCTCCATACTGTTCCAACAACATTCTTGTATTATTTGGAAGGCCTGCAAGAAATAATAGACTGGCACAAAATCATCAAACTTCAATCCAAGGATGGATCATTCCTCAGCTCGCCCGCATCTACAGCAGCTGTATTCATGCGTACAGGGAACACCAAGTGCTTGGAATTCCTGAACTTCGTTTTAATCAAATTCGGAAACCATGTGCCGTGTCACTATCCGCTTGATCTATTTGAACGTCTGTGGGCCGTTGATACTGTCGAACGGCTAGGGATCGATCGCCATTTCAAAAAGGAAATCAAGGACGCATTGGATTATGTTTACAGCCATTGGGACGAAAGAGGCATTGGATGGGCGAGAGAGAATCCTGTTGCTTATATTGATGTTATGGCCACGGGACTTCGAATCTTGAGACTGCATAGATACAATGTATCCTCAGACATTTTAAAAACTTTTTAGAGATGAGAATGGGGAGTTCTATCGCTTCCCGGGTCAAAGGGAGAGAGGAGTAACAGACATGTTAAACCTCAATCGTTGCTCACACGTTGCATTTCCCGGAGAAACGTTCATGGAAGAAGCAAAACTCTGTACCGAAAGGTATCTGCGGAACGCTTTGGAAAATGTGAATCCGTTAGACAAATGGGGTCTAAAAGAGAACATCCGGGGAGAGGTGGAGTACGCCCTCAAATATCCATGGCTTAGGAGACTGCCAAGGCTGGAGGCCAGAAACTACATTGAACATTACGGGGCAAACGATGTTTGGCTTGGGAAAATGATGCATACGATGCCATATATTAACAACCGCAAATACTTGGAACTGGCAAAACTGGACTTCAATAGTGTGCAGTCTATACACCAAAAGGAGTTACGCGAGCTTCGAAGATGGTGGAAATCATCAGGTTTAGCAGAGCTCAATTTTCTACCCGATCGTGTGGCTGAAATATTTTTCACAATCGCGTCTTCTATGTTTGAACCCGAGCTCGCTACGTGTAGAGCCGTTTATACGAAATCTACCATCTGCACTGTCATTTTAGATGCCTTCTATGACGTGCATGGATCTGCAGAAGACATCATGCTGTTCAATGAAGCAGTTAAAAGATGGGATCACTCTTTGTTAGACCGAATGCCAGAGCACATCAAAACATGTTTCCTGGCTCTGTACAATGTGGTTAATGAAATAGCAGAAGAAGGGCGTAAGAGACAGGGTCATGACGTGCTCCCCTACATTAGAAATTTATGGGAGATCCAGCTTGAATCTTTCACGAAAGAAGCAGAATGGTCGAAAGCTGAACATGTGCCGTCCTTCCATGAATACATAGAGGCCGCTACCATTTCAGTAGCGCTGCCAACTCTCATTCTGATTAGTGTTATTTTCACCGGAGAGGTTCTCACAGATCACATACTCTCCCAAATTGATTACCGATCCAAATTTGCATATCTCATGAGCTTGACAGGGCGTTTGGCGAACGATACCAAAACTTACCAGGTGGAGAGAGGTCGAGGAGAGGTGGCTTCTGCCATACAGTGTTACATGAAGGAAAATCCCGAATTGTCAGAGGAAGAAGCTCTCGAATATATTTATAGGCTCATGGAAAACGCCCTCGCAGATTTCAAGGGTGAATTTCTCAAGACAAAAGACGTGCCGGAGTATTGCAGAAGACTGGTTTTTGACAATGCGAGATCAATGCAGCTCGTTTACATGGAAGGAGACGGTTTCAAACTTTCACATGAAACGGAAATTAAAGAGCAAGTCAAAAAGATCCTGTTCGAACCAGTTGCGTAGAATAAATTATTCAAATAAATGCCCGCCCCTCTCACGGTGTTCTGTTAACGTTCTAGGAACGCACGCCTGCTCAGCACCTGTTATAGTCGTTT

Ace file of the UniGene sp_v3.0_unigene2998

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Levopimaradiene synthase, chloroplastic n=2 Tax=Pinus RepID=TPSD1_PINTA	-	-	0.0	76%
FL-Next	sp=Levopimaradiene synthase, chloroplastic; Pinus taeda (Loblolly pine).	-	-	0.0	79%
Sma3	Copalyl diphosphate synthase	-	-	2.891e-34	-

Source	ECs	Term	Type	e value	Identity
Sma3	Levopimaradiene synthase.	EC:4.2.3.32	-	2.513e-11	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Diterpenoid biosynthesis	00904		2.513e-11	%
Sma3	Biosynthesis of secondary metabolites	01110		2.513e-11	%
Sma3	Copalyl diphosphate synthase.	EC:5.5.1.12	-	2.138e-18	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Diterpenoid biosynthesis	00904		2.138e-18	%
Sma3	Biosynthesis of terpenoids and steroids	01062		2.138e-18	%
Sma3	Metabolic pathways	01100		2.138e-18	%
Sma3	Biosynthesis of secondary metabolites	01110		2.138e-18	%
Sma3	Ent-copalyl diphosphate synthase.	EC:5.5.1.13	-	1.282e-17	-

Source	KEGGs	Term	e value	Identity
Sma3	Diterpenoid biosynthesis	00904	1.282e-17	%
Sma3	Biosynthesis of terpenoids and steroids	01062	1.282e-17	%
Sma3	Biosynthesis of plant hormones	01070	1.282e-17	%
Sma3	Metabolic pathways	01100	1.282e-17	%
Sma3	Biosynthesis of secondary metabolites	01110	1.282e-17	%

Source	Gene names
Sma3	ABS; AN1; An2; At4g02780; CPS; CPS1; CPS2; CPS4; CPSL1; CYC1; CYC2; CmCPS1; Cpps1; DTS2; GA1; GSVIVT00002095001; GSVIVT00023721001; GSVIVT00023723001; KSL4; LOC_Os02g17780; LOC_Os04g10060; LPS; LsCPS1; MtrDRAFT_AC155888g1v2; OSJNBa0035G04.5; OSJNBa0052P16

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	magnesium ion binding	GO:0000287	Molecular Function	-
Sma3	ent-copalyl diphosphate synthase activity	GO:0009905	Molecular Function	-
Sma3	lyase activity	GO:0016829	Molecular Function	-
Sma3	carbon-oxygen lyase activity, acting on phosphates	GO:0016838	Molecular Function	-
Sma3	manganese ion binding	GO:0030145	Molecular Function	-
Sma3	abietadiene synthase activity	GO:0050554	Molecular Function	-
Sma3	copalyl diphosphate synthase activity	GO:0050559	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	gibberellin metabolic process	GO:0009685	Biological Process	-
Sma3	gibberellin biosynthetic process	GO:0009686	Biological Process	-
Sma3	gibberellic acid mediated signaling pathway	GO:0009740	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Prenyltransferase/squalene oxidase	IPR001330	-	-
Sma3	Terpene synthase-like	IPR001906	-	-
Sma3	Terpene synthase, metal-binding domain	IPR005630	-	-
Sma3	Terpenoid synthase	IPR008949	-	-
Sma3	DNA ligase, ATP-dependent, conserved site	IPR016059	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G02780.1	GA1, ABC33, ATCPS1, CPS, CPS1 Terpenoid cyclases/Protein prenyltransferases superfamily protein chr4:1237881-1244766 REVERSE LENGTH=802	46%
RefSeq	Arabidopsis thaliana	NP_192187.1	Ent-copalyl diphosphate synthase [Arabidopsis thaliana]	46%
RefSeq	Populus trichocarpa	XP_002302110.1	copalyl diphosphate synthase [Populus trichocarpa]	45%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: Q50EK2

Fln msg: Overlapping hits, possible frame ERROR between 1285 and 1278,

Fln protein: MALPCSSLSSHIHTGAHHHGNTQRIPHFHSSFNAGTSAGKRRNLYQRWGTKGSRKIVACVGEDPTARVKPEFSPSFWEDHIESLMPSYKVAPSDEKRIETLISEIKNMFRSMGYGETNPSAYDTAWVAKIPALDGSDYPHFPQTLQWLLQNQLKDGSWGEENYFSTYDRLLATLACIITLTVWRTGQTQVRQGIEFFKKHAGTMEDEADNHQPIGFEFVFPAMINEAKSLCLDLPYDMPFIKQIIEKREAKLKRIPTDTLHTVPTTFLYYLEGLQEIIDWHKIIKLQSKDGSFLSSPASTAAVFMRTGNTKCLEFLNFVLIKFGNHVPCHYPLDLFERLWAVDTVERLGIDRHFKKEIKDALDYVYSHWDERGIGWARENPVAYIDVMATGLRILRLHRYNVSSDILxxxxRDENGEFYRFPGQRERGVTDMLNLNRCSHVAFPGETFMEEAKLCTERYLRNALENVNPLDKWGLKENIRGEVEYALKYPWLRRLPRLEARNYIEHYGANDVWLGKMMHTMPYINNRKYLELAKLDFNSVQSIHQKELRELRRWWKSSGLAELNFLPDRVAEIFFTIASSMFEPELATCRAVYTKSTICTVILDAFYDVHGSAEDIMLFNEAVKRWDHSLLDRMPEHIKTCFLALYNVVNEIAEEGRKRQGHDVLPYIRNLWEIQLESFTKEAEWSKAEHVPSFHEYIEAATISVALPTLILISVIFTGEVLTDHILSQIDYRSKFAYLMSLTGRLANDTKTYQVERGRGEVASAIQCYMKENPELSEEEALEYIYRLMENALADFKGEFLKTKDVPEYCRRLVFDNARSMQLVYMEGDGFKLSHETEIKEQVKKILFEPVA*

Protein Length: 854

Fln nts: GACATATCATCTGATTGAGTTCAATATCTCTGCAACTGGTGTTGCATTTAGGAAG_-_ATGGCCTTGCCTTGCTCTTCATTGTCATCCCACATTCATACTGGAGCTCACCACCATGGTAACACACAACGCATTCCACATTTCCACAGCTCGTTTAATGCAGGAACCAGTGCTGGCAAACGGAGAAATTTGTACCAACGATGGGGTACTAAAGGTTCAAGAAAGATTGTTGCTTGTGTTGGGGAAGATCCTACAGCACGGGTGAAACCAGAATTTTCTCCAAGTTTTTGGGAGGACCATATCGAATCCCTAATGCCATCGTACAAGGTTGCACCATCGGACGAGAAGCGTATCGAGACATTAATATCCGAGATTAAGAATATGTTTCGATCTATGGGCTATGGGGAAACTAATCCCTCTGCATATGACACTGCTTGGGTAGCAAAGATTCCAGCACTTGATGGCTCTGACTACCCTCACTTTCCTCAAACACTCCAATGGCTTCTTCAAAATCAGTTGAAAGATGGCTCTTGGGGAGAAGAGAACTACTTCTCAACATATGACAGACTACTCGCTACCCTCGCGTGTATTATTACTCTCACTGTATGGCGCACTGGCCAGACACAAGTGCGGCAAGGTATTGAATTCTTCAAAAAGCACGCTGGAACGATGGAGGATGAGGCGGACAATCACCAGCCAATTGGATTTGAATTTGTCTTTCCTGCAATGATAAATGAAGCTAAAAGCTTATGCTTGGATTTGCCTTATGATATGCCATTCATCAAACAAATCATTGAGAAGCGGGAGGCTAAACTTAAAAGGATTCCCACTGATACTCTCCATACTGTTCCAACAACATTCTTGTATTATTTGGAAGGCCTGCAAGAAATAATAGACTGGCACAAAATCATCAAACTTCAATCCAAGGATGGATCATTCCTCAGCTCGCCCGCATCTACAGCAGCTGTATTCATGCGTACAGGGAACACCAAGTGCTTGGAATTCCTGAACTTCGTTTTAATCAAATTCGGAAACCATGTGCCGTGTCACTATCCGCTTGATCTATTTGAACGTCTGTGGGCCGTTGATACTGTCGAACGGCTAGGGATCGATCGCCATTTCAAAAAGGAAATCAAGGACGCATTGGATTATGTTTACAGCCATTGGGACGAAAGAGGCATTGGATGGGCGAGAGAGAATCCTGTTGCTTATATTGATGTTATGGCCACGGGACTTCGAATCTTGAGACTGCATAGATACAATGTATCCTCAGACATTTTAxxxxxxxxxxxxAGAGATGAGAATGGGGAGTTCTATCGCTTCCCGGGTCAAAGGGAGAGAGGAGTAACAGACATGTTAAACCTCAATCGTTGCTCACACGTTGCATTTCCCGGAGAAACGTTCATGGAAGAAGCAAAACTCTGTACCGAAAGGTATCTGCGGAACGCTTTGGAAAATGTGAATCCGTTAGACAAATGGGGTCTAAAAGAGAACATCCGGGGAGAGGTGGAGTACGCCCTCAAATATCCATGGCTTAGGAGACTGCCAAGGCTGGAGGCCAGAAACTACATTGAACATTACGGGGCAAACGATGTTTGGCTTGGGAAAATGATGCATACGATGCCATATATTAACAACCGCAAATACTTGGAACTGGCAAAACTGGACTTCAATAGTGTGCAGTCTATACACCAAAAGGAGTTACGCGAGCTTCGAAGATGGTGGAAATCATCAGGTTTAGCAGAGCTCAATTTTCTACCCGATCGTGTGGCTGAAATATTTTTCACAATCGCGTCTTCTATGTTTGAACCCGAGCTCGCTACGTGTAGAGCCGTTTATACGAAATCTACCATCTGCACTGTCATTTTAGATGCCTTCTATGACGTGCATGGATCTGCAGAAGACATCATGCTGTTCAATGAAGCAGTTAAAAGATGGGATCACTCTTTGTTAGACCGAATGCCAGAGCACATCAAAACATGTTTCCTGGCTCTGTACAATGTGGTTAATGAAATAGCAGAAGAAGGGCGTAAGAGACAGGGTCATGACGTGCTCCCCTACATTAGAAATTTATGGGAGATCCAGCTTGAATCTTTCACGAAAGAAGCAGAATGGTCGAAAGCTGAACATGTGCCGTCCTTCCATGAATACATAGAGGCCGCTACCATTTCAGTAGCGCTGCCAACTCTCATTCTGATTAGTGTTATTTTCACCGGAGAGGTTCTCACAGATCACATACTCTCCCAAATTGATTACCGATCCAAATTTGCATATCTCATGAGCTTGACAGGGCGTTTGGCGAACGATACCAAAACTTACCAGGTGGAGAGAGGTCGAGGAGAGGTGGCTTCTGCCATACAGTGTTACATGAAGGAAAATCCCGAATTGTCAGAGGAAGAAGCTCTCGAATATATTTATAGGCTCATGGAAAACGCCCTCGCAGATTTCAAGGGTGAATTTCTCAAGACAAAAGACGTGCCGGAGTATTGCAGAAGACTGGTTTTTGACAATGCGAGATCAATGCAGCTCGTTTACATGGAAGGAGACGGTTTCAAACTTTCACATGAAACGGAAATTAAAGAGCAAGTCAAAAAGATCCTGTTCGAACCAGTTGCGTAG___AATAAATTATTCAAATAAATGCCCGCCCCTCTCACGGTGTTCTGTTAACGTTCTAGGAACGCACGCCTGCTCAGCACCTGTTATAGTCGTTT

Fln Alignment:

Contig2998___MALPCSSLSSHIHTGAHHHGNTQRIPHFHSSFNAGTSAGKRRNLYQRWGTKGSRKIVACVGEDPTA---RVKPEFSPSFWEDH-IESLMPSYKVAPSDEKRIETLISEIKNMFRSMGYGETNPSAYDTAWVAKIPALDGSDYPHFPQTLQWLLQNQLKDGSWGEENYFSTYDRXXXXXXXXXXXXVWRTGQTQVRQGIEFFKKHAGTMEDEADNHQPIGFEFVFPAMINEAKSLCLDLPYDMPFIKQIIEKREAKLKRIPTDTLHTVPTTFLYYLEGLQEIIDWHKIIKLQSKDGSFLSSPASTAAVFMRTGNTKCLEFLNFVLIKFGNHVPCHYPLDLFERLWAVDTVERLGIDRHFKKEIKDALDYVYSHWDERGIGWARENPVAYIDVMATGLRILRLHRYNVSSDILxxxRDENGEFYRFPGQRERGVTDMLNLNRCSHVAFPGETFMEEAKLCTERYLRNALENVNPLDKWGLKENIRGEVEYALKYPWLRRLPRLEARNYIEHYGANDVWLGKMMHTMPYINNRKYLELAKLDFNSVQSIHQKELRELRRWWKSSGLAELNFLPDRVAEIFFTIASSMFEPELATCRAVYTKSTICTVILDAFYDVHGSAEDIMLFNEAVKRWDHSLLDRMPEHIKTCFLALYNVVNEIAEEGRKRQGHDVLPYIRNLWEIQLESFTKEAEWSKAEHVPSFHEYIEAATISVALPTLILISVIFTGEVLTDHILSQIDYRSKFAYLMSLTGRLANDTKTYQVERGRGEVASAIQCYMKXXXXXXXXXXXXYIYRLMENALADFKGEFLKTKDVPEYCRRLVFDNARSMQLVYMEGDGFKLSHETEIKEQVKKILFEPVA
Q50EK2______________MALPSSSLSSQIHTGA----TTQCIPHFHGSLNAGTSAGKRRSLYLRWG-KGPSKIVACAGQDPFSVPTLVKREFPPGFWKDHVIESLMPSYKVAPSDEKRIETLITEIKNMFRSMGYGETNPSAYDTAWVARIPAVDGSEKPQFPETLEWILQNQLKDGSWGEEFYFLAYDRILATLACIITLTIWQTGDTQVQKGIEFFKTQAGKIEEEADSHRPSGFEIVFPAMLKEAKALGLALPYELPFIQQIIEKREAKLQRLPPDLLYALPTTLLYSLEGLQEIVDWEKIMKLQSKDGSFLSSPASTAAVFMRTGNKKCLEFLNFVLKKFGNHVPCHYPLDLFERLWAVDTVERLGIDHHFKEEIKDALDYVYSHWDERGIGWARENPVPDIDDTAMGLRILRLHGYNVSSDVLxxxRDENGEFFCFLGQTQRGVTDMLNVNRCSHVAFPGETIMEEAKLCTERYLRNALEDGGASDKWALKKNIRGEVEYALKYPWHRSMPRLEARSYIENYGPNDVWLGKTMYMMPNISNEKYLELAKLDFNRVQFFHRQELQDIRRWWNSSGFSQLGFTRERVAEIYFSPASFLFEPEFATCRAVYTKTSNFTVILDDLYDAHGTLDNLKLFSESVKRWDLSLVDQMPQDMKICFKGFYNTFNEIAEEGRKRQGRDVLSYIQKVWEVQLEAYTKEAEWSAVRYVPSYDEYIGNASVSIALGTVVLISALFTGEILTDDILSKIGRDSRFLYLMGLTGRLVNDTKTYQAERGQGEVASAVQCYMKDHPEISEEEALKHVYTIMDNALDELNREFVNNRDVPDTCRRLVFETARIMQLFYMDGDGLTLSHNMEIKEHVKNCLFQPVA

SNPs (tot: 4)

Position	A %	C %	G %	T %	Probability
1861	16			83	0.994512
1862		16		66	0.988813
1863			16	83	0.994512
2534			40	60	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain