SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene2729

Length: 2104 nt

UniGene Fasta

>sp_v3.0_unigene2729
CAACGATTATAAGGATCCGGAAGAAGAACAGAGCTTCAGAGCTTAATGATCATGGCGGGATCGGATACTCTTGACAATGGGAGCGGGGATGGGGAGCCTTCGTATTCTTTTAGATCCAAGCTTCCTGATATTGAGATCTCCAGCCATCTTTCTCTGCACGATTATTGTTTCGAGAGATTAGGGGAATTTGCAGAGAGGCCATGCATTTTACAGGGATCGAATGGGAAGGTTTATTCCTATGGAGAAGTCGATTTGATTTCTCGCAGGGTCGGCTCTGGTTTGGCCAAGCTCGGCATCGGGCATGGAGATGTCGTTATGCTTTTGTTGCCCAATTGCCCAGAGTTCGCTTTCGTTTACTTGGGTTCGTCTCGAATTGGCGCGATTGTGACGACTGCCAATCCTTTCTATAGGCCGGGCGAGATTGCGAAGCAGGCAATGGCGTCCGGGGCTCGGGTTATCGTCACTCAGGCCTCCTATGTTGAGAAATTGTGCGATCTTGAAAGGGAAAAGGGTCAGTTCTGGTTATTACAATTGACACAGGGGTTCCGGAGGGGTGCCTGCATTTCTCGGTTCTGGCAGAGGCCGACGAGAATGAGATACCCTTTGTCGAGATTCGACCTCATGACCTGGTGGCTCTGCCCTATTCCTCTGGAACGACTGGGCTTCCCAAGGGCGTGATGCTCACGCACAAGGCGCTCGTTTCTTCCGTTGCGCAGCACGTGGACGGCGAGAATCCCAATCTGTACCTGCATTCCGAGGACGTGATTCTATGCGTGCCGCCCTTGTTTCATATTTTTGGTAACACCCTGCTGCTCGGTTCTCTCAGAGTGGGCGCGGCTGTGTTGATTATGCAGAGATACGAAATCCATGCGCTGCTGGAGCTGATACAGAAATTCAAGGTCACTGTGGCTCCGTTCGTGCCTCCGATTGTTCTGGCCATTGCCAGGAATCCGATAGTGGAAAAATATGATATGTCGTCTATCAGAATGGTCATGTCAGGCGCAGCGCCTCTCGGGAAGGAGCTCGCAGACGCCTTTAGGGCTAGGATTCCCAACGCAACTTTGGCCGAGGGTTATGGCATGACAGAGGCCGGACCAGTTGCCCTGTCTCTGGGTTTGGCAAAAGTGCCCTTCAGCTTAAAGCCTGGTTCCTGCGGTACCGTGGTTAGAAATGCGGAAGTGAAGATAGTCGAACTCGAGACCGGAGTCTCCCTGCCACATAACCAGGCGGGAGAGATCTGTATCCGCGGCCCTCAAATTATGAAAGGGTATCTGAATGATCCGGAGGCGACTGAGATGACTATTGATAAAAATGGATGGTTGCATACCGGAGATGTTGGTTACATTGACGATGATGAAGAACTTTTCTTAGTGGACCGCGTCAAGGAACTGATCAAATACAAAGGGTTCCAGGTGCCACCGGCGGAACTGGAAGCGATTCTTTTGAGCCATCCATCCATAGCCGACGCAGCAGTTGTGTCGCAGCAGGATGAAATTGCTGGAGAGGTGCCGGTAGCCTTTGTGGTGAGATCTGGTGATGGGATTGATTTAACGGTTAATGAGATTAAAGATTTCGTTGCCAAACAGGTGGTGTTCTACAAGAAGCTGCACAACGTTTATTTTATAGAAGCCATACCCAAATCTCCATCTGGCAAGATACTGCGCAAGGATTTAAGAGCCAAGCTTATGGCTGTCGCTGCAGGCATTCAATAATTTCAGCGGCCATGGCAGAAGGAGCCTTATCTGCTTTTATATTAAAATATATACAGATTTTATGTATTTTCTGGTGTCTCGCCAGCAGGATTGACCTGGTAGTGTAAGTTGTGAAAGTTTACTTATTCATTTATTTCCTCGTTCATTTTTAGCTGGCCTGAGATGACTCTGGACATGTTTCTAATTATAGTGTTGCTGCTGCCATGGTATGCATGGCATGACATGACAGAGTTGTTGTCAGTTTATTCCATTTTTAAAAATGGGTAAGCGGCCTTTGGTTGTTGGTAACACATTCATCGTGTGACGATGAATGGCAAATCAGTGTGACTCAGCTCATGTAATACTTTTTTTATAAATGTTGGTTCCATAATCATTGAGTTATTATATTCTCG

Ace file of the UniGene sp_v3.0_unigene2729

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	4-coumarate--CoA ligase n=18 Tax=Pinaceae RepID=4CL_PINTA	-	-	72%
FL-Next	tr=4-coumarate:CoA ligase; Pinus massoniana.	-	-	66%
Sma3	4-coumarate:CoA ligase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing).	EC:1.13.12.7	-	1.728e-35	-
Sma3	Ligases, Forming carbon-sulfur bonds, Acid--thiol ligases.	EC:6.2.1.-	-	1.143e-28	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Geraniol degradation	00281		1.143e-28	%
Sma3	Tyrosine metabolism	00350		1.143e-28	%
Sma3	Benzoate degradation	00362		1.143e-28	%
Sma3	Fluorobenzoate degradation	00364		1.143e-28	%
Sma3	alpha-Linolenic acid metabolism	00592		1.143e-28	%
Sma3	Aminobenzoate degradation	00627		1.143e-28	%
Sma3	Propanoate metabolism	00640		1.143e-28	%
Sma3	Ethylbenzene degradation	00642		1.143e-28	%
Sma3	Carbon fixation pathways in prokaryotes	00720		1.143e-28	%
Sma3	Limonene and pinene degradation	00903		1.143e-28	%
Sma3	Tropane, piperidine and pyridine alkaloid biosynthesis	00960		1.143e-28	%
Sma3	Biosynthesis of plant hormones	01070		1.143e-28	%
Sma3	Metabolic pathways	01100		1.143e-28	%
Sma3	Biosynthesis of secondary metabolites	01110		1.143e-28	%
Sma3	4-coumarate--CoA ligase.	EC:6.2.1.12	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Ubiquinone and other terpenoid-quinone biosynthesis	00130	%
Sma3	Phenylalanine metabolism	00360	%
Sma3	Phenylpropanoid biosynthesis	00940	%
Sma3	Biosynthesis of phenylpropanoids	01061	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	%
Sma3	Metabolic pathways	01100	%
Sma3	Biosynthesis of secondary metabolites	01110	%

Source	Gene names
Sma3	4CL; 4CL-1; 4CL-2; 4CL-2a; 4CL-2b; 4CL.2; 4CL1; 4CL2; 4CL3; 4CL4; 4CL5; 4CLL1; 4CLL2; 4CLL3; 4CLL4; 4CLL5; 4CLL6; 4CLL7; 4CLL9; 4cl; 4cl1; 4cl2; ACLL13; ACLL14; ACLL18\|Ptr4CL13; ACLL20; ACLL21; At1g20480; At1g20490; At1g51680; At1g62940; At1g65060; At3g21

Source	GOs	Term	Type	Identity
Sma3	peroxisome	GO:0005777	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	fatty-acyl-CoA synthase activity	GO:0004321	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	4-coumarate-CoA ligase activity	GO:0016207	Molecular Function	-
Sma3	ligase activity	GO:0016874	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	jasmonic acid biosynthetic process	GO:0009695	Biological Process	-
Sma3	phenylpropanoid metabolic process	GO:0009698	Biological Process	-
Sma3	phenylpropanoid biosynthetic process	GO:0009699	Biological Process	-
Sma3	oxylipin biosynthetic process	GO:0031408	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Regulator of chromosome condensation, RCC1	IPR000408	-	-
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	-
Sma3	Adenosine/AMP deaminase active site	IPR006650	-	-
Sma3	HTH domain AraC-type, conserved site	IPR018062	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G51680.1	4CL1, 4CL.1, AT4CL1 4-coumarate:CoA ligase 1 chr1:19159007-19161464 REVERSE LENGTH=561	67%
RefSeq	Arabidopsis thaliana	NP_175579.1	4-coumarate--CoA ligase 1 [Arabidopsis thaliana]	67%
RefSeq	Populus trichocarpa	XP_002329649.1	4-Coumarate:CoA ligase [Populus trichocarpa]	68%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C1KBY2

Fln msg: Separated hits, possible frame ERROR between 501 and 516,

Fln protein: MIMAGSDTLDNGSGDGEPSYSFRSKLPDIEISSHLSLHDYCFERLGEFAERPCILQGSNGKVYSYGEVDLISRRVGSGLAKLGIGHGDVVMLLLPNCPEFAFVYLGSSRIGAIVTTANPFYRPGEIAKQAMASGARVIVTQASYVEKLCDLxxxxxxVLVITIDTGVPEGCLHFSVLAEADENEIPFVEIRPHDLVALPYSSGTTGLPKGVMLTHKALVSSVAQHVDGENPNLYLHSEDVILCVPPLFHIFGNTLLLGSLRVGAAVLIMQRYEIHALLELIQKFKVTVAPFVPPIVLAIARNPIVEKYDMSSIRMVMSGAAPLGKELADAFRARIPNATLAEGYGMTEAGPVALSLGLAKVPFSLKPGSCGTVVRNAEVKIVELETGVSLPHNQAGEICIRGPQIMKGYLNDPEATEMTIDKNGWLHTGDVGYIDDDEELFLVDRVKELIKYKGFQVPPAELEAILLSHPSIADAAVVSQQDEIAGEVPVAFVVRSGDGIDLTVNEIKDFVAKQVVFYKKLHNVYFIEAIPKSPSGKILRKDLRAKLMAVAAGIQ*

Protein Length: 557

Fln nts: CAACGATTATAAGGATCCGGAAGAAGAACAGAGCTTCAGAGCTTA_-_ATGATCATGGCGGGATCGGATACTCTTGACAATGGGAGCGGGGATGGGGAGCCTTCGTATTCTTTTAGATCCAAGCTTCCTGATATTGAGATCTCCAGCCATCTTTCTCTGCACGATTATTGTTTCGAGAGATTAGGGGAATTTGCAGAGAGGCCATGCATTTTACAGGGATCGAATGGGAAGGTTTATTCCTATGGAGAAGTCGATTTGATTTCTCGCAGGGTCGGCTCTGGTTTGGCCAAGCTCGGCATCGGGCATGGAGATGTCGTTATGCTTTTGTTGCCCAATTGCCCAGAGTTCGCTTTCGTTTACTTGGGTTCGTCTCGAATTGGCGCGATTGTGACGACTGCCAATCCTTTCTATAGGCCGGGCGAGATTGCGAAGCAGGCAATGGCGTCCGGGGCTCGGGTTATCGTCACTCAGGCCTCCTATGTTGAGAAATTGTGCGATCTTGAxxxxxxxxxxxxxxxAGTTCTGGTTATTACAATTGACACAGGGGTTCCGGAGGGGTGCCTGCATTTCTCGGTTCTGGCAGAGGCCGACGAGAATGAGATACCCTTTGTCGAGATTCGACCTCATGACCTGGTGGCTCTGCCCTATTCCTCTGGAACGACTGGGCTTCCCAAGGGCGTGATGCTCACGCACAAGGCGCTCGTTTCTTCCGTTGCGCAGCACGTGGACGGCGAGAATCCCAATCTGTACCTGCATTCCGAGGACGTGATTCTATGCGTGCCGCCCTTGTTTCATATTTTTGGTAACACCCTGCTGCTCGGTTCTCTCAGAGTGGGCGCGGCTGTGTTGATTATGCAGAGATACGAAATCCATGCGCTGCTGGAGCTGATACAGAAATTCAAGGTCACTGTGGCTCCGTTCGTGCCTCCGATTGTTCTGGCCATTGCCAGGAATCCGATAGTGGAAAAATATGATATGTCGTCTATCAGAATGGTCATGTCAGGCGCAGCGCCTCTCGGGAAGGAGCTCGCAGACGCCTTTAGGGCTAGGATTCCCAACGCAACTTTGGCCGAGGGTTATGGCATGACAGAGGCCGGACCAGTTGCCCTGTCTCTGGGTTTGGCAAAAGTGCCCTTCAGCTTAAAGCCTGGTTCCTGCGGTACCGTGGTTAGAAATGCGGAAGTGAAGATAGTCGAACTCGAGACCGGAGTCTCCCTGCCACATAACCAGGCGGGAGAGATCTGTATCCGCGGCCCTCAAATTATGAAAGGGTATCTGAATGATCCGGAGGCGACTGAGATGACTATTGATAAAAATGGATGGTTGCATACCGGAGATGTTGGTTACATTGACGATGATGAAGAACTTTTCTTAGTGGACCGCGTCAAGGAACTGATCAAATACAAAGGGTTCCAGGTGCCACCGGCGGAACTGGAAGCGATTCTTTTGAGCCATCCATCCATAGCCGACGCAGCAGTTGTGTCGCAGCAGGATGAAATTGCTGGAGAGGTGCCGGTAGCCTTTGTGGTGAGATCTGGTGATGGGATTGATTTAACGGTTAATGAGATTAAAGATTTCGTTGCCAAACAGGTGGTGTTCTACAAGAAGCTGCACAACGTTTATTTTATAGAAGCCATACCCAAATCTCCATCTGGCAAGATACTGCGCAAGGATTTAAGAGCCAAGCTTATGGCTGTCGCTGCAGGCATTCAATAA___TTTCAGCGGCCATGGCAGAAGGAGCCTTATCTGCTTTTATATTAAAATATATACAGATTTTATGTATTTTCTGGTGTCTCGCCAGCAGGATTGACCTGGTAGTGTAAGTTGTGAAAGTTTACTTATTCATTTATTTCCTCGTTCATTTTTAGCTGGCCTGAGATGACTCTGGACATGTTTCTAATTATAGTGTTGCTGCTGCCATGGTATGCATGGCATGACATGACAGAGTTGTTGTCAGTTTATTCCATTTTTAAAAATGGGTAAGCGGCCTTTGGTTGTTGGTAACACATTCATCGTGTGACGATGAATGGCAAATCAGTGTGACTCAGCTCATGTAATACTTTTTTTATAAATGTTGGTTCCATAATCATTGAGTTATTATATTCTCG

Fln Alignment:

Contig2729___SGDGEPSYSFRSKLPDIEISSHLSLHDYCFERLGEFAERPCILQGSNGKVYSYGEVDLISRRVGSGLAKLGIGHGDVVMLLLPNCPEFAFVYLGSSRIGAIVTTANPFYRPGEIAKQAMASGARVIVTQASYVEKLCDLExxxxxVLVITIDTGVPEGCLHFSVLAEADENEIPFVEIRPHDLVALPYSSGTTGLPKGVMLTHKALVSSVAQHVDGENPNLYLHSEDVILCVPPLFHIFG-NTLLLGSLRVGAAVLIMQRYEIHALLELIQKFKVTVAPFVPPIVLAIARNPIVEKYDMSSIRMVMSGAAPLGKELADAFRARIPNATLAEGYGMTEAGPV-ALSLGLAKVPFSLKPGSCGTVVRNAEVKIVELETGVSLPHNQAGEICIRGPQIMKGYLNDPEATEMTIDKNGWLHTGDVGYIDDDEELFLVDRVKELIKYKGFQVPPAELEAILLSHPSIADAAVVSQQDEIAGEVPVAFVVRSGDGIDLTVNEIKDFVAKQVVFYKKLHNVYFIEAIPKSPSGKILRKDLRAKLMA
C1KBY2______________NGIKKVEHLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYSFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQxxxxxVLVITIDDAPKEGCQHISVLTEADETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSEDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEETGEVPVAFVVKSS---EISEQEIKEFVAKQVVFYKKIHRVYFVDAIPKSPSGKILRKDLRSRLAA

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
274		85		14	0.993533
296	85			14	0.993533
466		20		80	0.995493
481		20		80	0.995493
1558			20	80	0.995493
1573	25			75	0.996473
1638		85		14	0.993533
2099	20			80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain