SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene2547

Length: 2924 nt

UniGene Fasta

>sp_v3.0_unigene2547
GCAGATAATAAAGATGATAATGAAGAAGACAGTCTTAACTTAACAGTGATAGCAGCTGACACTGATTCCCATACAAATATAGAACGCAATAAAGCTGGAAACATCTCGAATACTTTGTGTCTTCAATCTCTTATATCGCCACGGTCTTCAACTTCTAGGGCTTCGAGAAGTTCATCTGTTGTATCCATTTCTCGCAATCACAATTCTTCATCAGTCTCACCCTCAGCCTCACCTTTGGCATCATTTCGCAGTTCTCCACACAGATCAGTCAAGCCAGTTTCTGAAGCAAAGAAATCTGCTTCAGTGACTTTCCATAATGTAGAAGAGATGGAATCCGATTATATGTATTCATCTGAATTTAACTCTGCAGAACTGCGACCAGGTTCATCATATAATATGAGAAATGGTTATCACGGAGGATCAGGCGCAACAACAGAAGAAAGGGGTATGCCATAGATGCATGAAAGGAAATAGGTTGAAGGACAAGGAATCTTGTATTGTCTGTAATGCCAAGTATTGCAACAATTGTGTGCTAAGAGCAATGGGATCAATGCCCGAGGGGCGTAAATGTGTACCTTGCATTGGCCAGGCAATTGACGAGTCAAGGCGATCATCATTAGGGAAATGCTCCCGAATGTTGTGTCGTCTTCTTAATCCTTTAGAGGTTCAGCAAATTATGAAGGCTGAGAAAGAATGCTCAGCAAATCAGCTTCGACCAGAACAGCTATATGTCAATGAGAGGCAGCTGAGACCAGAAGAAATGGCTGAATTACTTGGCTGTCCTAAACCTCCCTCGAAGTTGAAACCAGGGAGATACTGGTACGATAAAGAATCTGGATTGTGGGGGAAGGAGGGTGAAAAGCCAGACAAGATTGTAAGTGCAAGCTTGAATATAGGGGGGAAGCTTCTACAAAATGCAAGTTACGGGAATACACAGGTTTACATCAATGGACGCGAGATTACCAAGACAGAGCTGAAGATGCTGAGGTTGGCAGGGGTGCAGTGCCCACGTGACACTCATTTCTGGGTGTATGCAAATGGATCATATGAAGAAGAAGGTCAGAATAATATAAAAGGAAACATATGGGGCAAGGCAGGCATTTACAAGGTTGATATGCTCTTTACTATCGTTACCCACCCCTCGGAGGAACTATCATGGCTCGAGAGAAAATACAGATAATCATTCCAGCAGATCTGTCCCAGAATACTTGGAGCAGAAAAAGATTCAAAAGCTCTTGTTACTTGGATATGAAGGCTCTGGCACTAGTACAATTTTCAAGCAGGCAAAATTTCTGTACAAGGATGAGAAATTTTCACAGGAGGAGCTGCAAAACATCAAACTGATGATACAGAGCAACCTATATAAATACCTTAGCATATTGCTTGAAGGGCGTGAGCGTTTTGAAGAGGAAAGTTTAGCAGATGTCAATGGTTCCAGTGTACGTGAACAAGTTTCTGCCTCAGATGAAAGTGATGATAAAGAAGCTGGTCAATGTATTTACTCTCTCAATCCAAAATTGAAACATTTTTCTGATTGGCTTCTGGAAATTATGGCAATGGGGGGATTTAGATGCTTTTTTCCCAGCTGCAACACGTGAGTATGCCCCAATTGTTGATGAAATGTGGAGGGACCCTGCAATTCAGGCTACATATGCTAGAAGAAATGAGCTGCATGTCCTCCCTGATGTTGCTGGTTACTTCTTGGAACGGGCTGTTGAGGTATCAAGTAATGAATACGATCCATCAGAATCTGACATTTTATATGCTGAAGGAGTGACTCAAAGTAATGGGCTGGCATTTATCGAATTTTCACTCGATGATCGTAGTCCAATGTCAGAGCCTTATAATGAAAACTTTGATTGTCCAACACCACCTGTGAAGTATCAGCTTATTAGAGTAAATACTAAAGGATTAAATGAAGGATGTAAGTGGCTGGAAATGTTTGAGGATGTACGAGCTGTAATCTTCTGTGTTTCTATAAGTGAATATGACCAAATGTGGGCTGAAGACAATGGTACCTTGAGAAATAAGATGATCTTGAGTAAAGAACTTTTTGAGAATGTTGTGAAGCATTCATGTTTCAGAGATACACCATTCATTCTCTTACTAAATAAATATGACATTTTTGAGGAGAAGATCAACAGAGTGCCACTTACTGTATGTGAGTGGTTTAAGGATTTCAGTCCAGTGAAGGCGCATCACAACAATCAGTCACTGGCCCATCAAGCTTACTATTATGTGGCAATGAAATTCAAGGATCTCTATGCGTCTCTTAGCAGCCATAAGCTGTTTGTATGGCAAATGAAGGCTCGAGATCGCAGTACAGTGAATGAAGCCTTCAAATATATTCGTGAGATTTTAAAGTGGGACGAAGAAAAGGATGAAAACTATTTGGGAATTGCTGATGAATCACTTTACAGCACTGATATGAGTTCTTCACCATTTGTCAGGCAAGAGGGATGATTCTTTGATTCATGGGAAGTGTCTCGGGCAGATTTACTATTTTGGGTTCCTTGGTTTCGTGTGATCCAGGAATTACTTGTATAATATATATATGTATTGAAATTCTAAAAATCTTTATTGGTTTTTTGGGAGGCAATATTCTAACTTATTTTTCTCGAGTGTTCGACAATCTTGCATTCTTTTGCGTAGTCATGTATTGAAGGGTGCTTTTTAAATTCGATTGGTCAGGTGCCACCAATCTTCGTCTTTGTACAGATAATAAAGTGCTTGTCATAGAGATAAATTCCAAGGCACAGAATTTGGGAAATCTGGGCACCGTTTGTGGCAGTAGAACTAATGGAGAGACACTTGATGCACTGTTTGTATTGTAAAACATTACTGAGCCATGTATTTTTACTTTTGATGTAAATTGTTTTGTGAACCTTCACAGCATTTAGTTTCAGTGATTGAATCTGCTTGGC

Ace file of the UniGene sp_v3.0_unigene2547

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	GTP-binding protein alpha subunit, gna, putative n=1 Tax=Ricinus communis RepID=B9T5D0_RICCO	-	-	0.0	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	39%
Sma3	GP-alpha-1	-	-	2.928e-17	-

Source	Gene names
Sma3	AT1G31930; AT4g34390; At1g31930; At2g23460; At2g23460/F26B6.11; At4g34390; D1; F5M6.7; GA1; GA2; GPA1; GPA2; GSVIVT00006612001; GSVIVT00013154001; GSVIVT00015638001; GSVIVT00036460001; Gpa1; HG1; HG2; LOC_Os10g02814; LOC_Os11g10050; LOC_Os12g40190; OSJNBa

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	signal transducer activity	GO:0004871	Molecular Function	-
Sma3	GTP binding	GO:0005525	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	guanyl nucleotide binding	GO:0019001	Molecular Function	-
Sma3	protein ADP-ribosylation	GO:0006471	Biological Process	-
Sma3	G-protein coupled receptor signaling pathway	GO:0007186	Biological Process	-
Sma3	gravitropism	GO:0009630	Biological Process	-
Sma3	thigmotropism	GO:0009652	Biological Process	-
Sma3	response to ethylene stimulus	GO:0009723	Biological Process	-
Sma3	response to abscisic acid stimulus	GO:0009737	Biological Process	-
Sma3	response to sucrose stimulus	GO:0009744	Biological Process	-
Sma3	response to glucose stimulus	GO:0009749	Biological Process	-
Sma3	response to fructose stimulus	GO:0009750	Biological Process	-
Sma3	response to mannitol stimulus	GO:0010555	Biological Process	-
Sma3	root development	GO:0048364	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Guanine nucleotide binding protein (G-protein), alpha subunit	IPR001019	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Plant G-protein, alpha subunit	IPR002976	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	G protein alpha subunit, helical insertion	IPR011025	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G31930.1	XLG3 extra-large GTP-binding protein 3 chr1:11465832-11468961 FORWARD LENGTH=848	69%
RefSeq	Arabidopsis thaliana	NP_001185125.1	extra-large GTP-binding protein 3 [Arabidopsis thaliana]	69%
RefSeq	Populus trichocarpa	XP_002303447.1	predicted protein [Populus trichocarpa]	75%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AB61

Fln msg: your sequence is longer than subject: 281 - 123

Fln protein: MWRDPAIQATYARRNELHVLPDVAGYFLERAVEVSSNEYDPSESDILYAEGVTQSNGLAFIEFSLDDRSPMSEPYNENFDCPTPPVKYQLIRVNTKGLNEGCKWLEMFEDVRAVIFCVSISEYDQMWAEDNGTLRNKMILSKELFENVVKHSCFRDTPFILLLNKYDIFEEKINRVPLTVCEWFKDFSPVKAHHNNQSLAHQAYYYVAMKFKDLYASLSSHKLFVWQMKARDRSTVNEAFKYIREILKWDEEKDENYLGIADESLYSTDMSSSPFVRQEG*

Protein Length: 282

Fln nts: GCAGATAATAAAGATGATAATGAAGAAGACAGTCTTAACTTAACAGTGATAGCAGCTGACACTGATTCCCATACAAATATAGAACGCAATAAAGCTGGAAACATCTCGAATACTTTGTGTCTTCAATCTCTTATATCGCCACGGTCTTCAACTTCTAGGGCTTCGAGAAGTTCATCTGTTGTATCCATTTCTCGCAATCACAATTCTTCATCAGTCTCACCCTCAGCCTCACCTTTGGCATCATTTCGCAGTTCTCCACACAGATCAGTCAAGCCAGTTTCTGAAGCAAAGAAATCTGCTTCAGTGACTTTCCATAATGTAGAAGAGATGGAATCCGATTATATGTATTCATCTGAATTTAACTCTGCAGAACTGCGACCAGGTTCATCATATAATATGAGAAATGGTTATCACGGAGGATCAGGCGCAACAACAGAAGAAAGGGGTATGCCATAGATGCATGAAAGGAAATAGGTTGAAGGACAAGGAATCTTGTATTGTCTGTAATGCCAAGTATTGCAACAATTGTGTGCTAAGAGCAATGGGATCAATGCCCGAGGGGCGTAAATGTGTACCTTGCATTGGCCAGGCAATTGACGAGTCAAGGCGATCATCATTAGGGAAATGCTCCCGAATGTTGTGTCGTCTTCTTAATCCTTTAGAGGTTCAGCAAATTATGAAGGCTGAGAAAGAATGCTCAGCAAATCAGCTTCGACCAGAACAGCTATATGTCAATGAGAGGCAGCTGAGACCAGAAGAAATGGCTGAATTACTTGGCTGTCCTAAACCTCCCTCGAAGTTGAAACCAGGGAGATACTGGTACGATAAAGAATCTGGATTGTGGGGGAAGGAGGGTGAAAAGCCAGACAAGATTGTAAGTGCAAGCTTGAATATAGGGGGGAAGCTTCTACAAAATGCAAGTTACGGGAATACACAGGTTTACATCAATGGACGCGAGATTACCAAGACAGAGCTGAAGATGCTGAGGTTGGCAGGGGTGCAGTGCCCACGTGACACTCATTTCTGGGTGTATGCAAATGGATCATATGAAGAAGAAGGTCAGAATAATATAAAAGGAAACATATGGGGCAAGGCAGGCATTTACAAGGTTGATATGCTCTTTACTATCGTTACCCACCCCTCGGAGGAACTATCATGGCTCGAGAGAAAATACAGATAATCATTCCAGCAGATCTGTCCCAGAATACTTGGAGCAGAAAAAGATTCAAAAGCTCTTGTTACTTGGATATGAAGGCTCTGGCACTAGTACAATTTTCAAGCAGGCAAAATTTCTGTACAAGGATGAGAAATTTTCACAGGAGGAGCTGCAAAACATCAAACTGATGATACAGAGCAACCTATATAAATACCTTAGCATATTGCTTGAAGGGCGTGAGCGTTTTGAAGAGGAAAGTTTAGCAGATGTCAATGGTTCCAGTGTACGTGAACAAGTTTCTGCCTCAGATGAAAGTGATGATAAAGAAGCTGGTCAATGTATTTACTCTCTCAATCCAAAATTGAAACATTTTTCTGATTGGCTTCTGGAAATTATGGCAATGGGGGGATTTAGATGCTTTTTTCCCAGCTGCAACACGTGAGTATGCCCCAATTGTTGATGAA_-_ATGTGGAGGGACCCTGCAATTCAGGCTACATATGCTAGAAGAAATGAGCTGCATGTCCTCCCTGATGTTGCTGGTTACTTCTTGGAACGGGCTGTTGAGGTATCAAGTAATGAATACGATCCATCAGAATCTGACATTTTATATGCTGAAGGAGTGACTCAAAGTAATGGGCTGGCATTTATCGAATTTTCACTCGATGATCGTAGTCCAATGTCAGAGCCTTATAATGAAAACTTTGATTGTCCAACACCACCTGTGAAGTATCAGCTTATTAGAGTAAATACTAAAGGATTAAATGAAGGATGTAAGTGGCTGGAAATGTTTGAGGATGTACGAGCTGTAATCTTCTGTGTTTCTATAAGTGAATATGACCAAATGTGGGCTGAAGACAATGGTACCTTGAGAAATAAGATGATCTTGAGTAAAGAACTTTTTGAGAATGTTGTGAAGCATTCATGTTTCAGAGATACACCATTCATTCTCTTACTAAATAAATATGACATTTTTGAGGAGAAGATCAACAGAGTGCCACTTACTGTATGTGAGTGGTTTAAGGATTTCAGTCCAGTGAAGGCGCATCACAACAATCAGTCACTGGCCCATCAAGCTTACTATTATGTGGCAATGAAATTCAAGGATCTCTATGCGTCTCTTAGCAGCCATAAGCTGTTTGTATGGCAAATGAAGGCTCGAGATCGCAGTACAGTGAATGAAGCCTTCAAATATATTCGTGAGATTTTAAAGTGGGACGAAGAAAAGGATGAAAACTATTTGGGAATTGCTGATGAATCACTTTACAGCACTGATATGAGTTCTTCACCATTTGTCAGGCAAGAGGGATGA___TTCTTTGATTCATGGGAAGTGTCTCGGGCAGATTTACTATTTTGGGTTCCTTGGTTTCGTGTGATCCAGGAATTACTTGTATAATATATATATGTATTGAAATTCTAAAAATCTTTATTGGTTTTTTGGGAGGCAATATTCTAACTTATTTTTCTCGAGTGTTCGACAATCTTGCATTCTTTTGCGTAGTCATGTATTGAAGGGTGCTTTTTAAATTCGATTGGTCAGGTGCCACCAATCTTCGTCTTTGTACAGATAATAAAGTGCTTGTCATAGAGATAAATTCCAAGGCACAGAATTTGGGAAATCTGGGCACCGTTTGTGGCAGTAGAACTAATGGAGAGACACTTGATGCACTGTTTGTATTGTAAAACATTACTGAGCCATGTATTTTTACTTTTGATGTAAATTGTTTTGTGAACCTTCACAGCATTTAGTTTCAGTGATTGAATCTGCTTGGC

Fln Alignment:

Contig2547___MILSKELFENVVKHSCFRDTPFILLLNKYDIFEEKINRVPLTVCEWFKDFSPVKAHHNNQSLAHQAYYYVAMKFKDLY-----ASLSSHKLFVWQMKARDRSTVNEAFKYIREIL
D5AB61______________MMETKELFDWVLKQPWFEKTSFLLFLNKFDIFETKVLKVPLNVCEWFRDYQPLTS--GKQEIEH-AYEFVKKKFEELYFQNTPADRVDRVFKIYRTTALDQKLIKKTFKLVDETL

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
1906	10	90			0.90298
1907		10		90	0.90298
1909	90			10	0.90298
1923			87	12	0.992554
1925	87		12		0.992554
1926			12	87	0.992554
2045		12		87	0.992554
2057		16		83	0.994512
2165		10		90	0.90298
2197		90		10	0.90298
2439		10		90	0.90298
2495			88	11	0.923564
2499		11	88		0.923564
2500	88	11			0.923564
2503			11	88	0.923564
2504	88		11		0.923564
2507	88		11		0.923564
2509		11		88	0.923564
2511		11		88	0.923564
2512	11		88		0.923564
2794		57	42		1.0
2859	12			25	1.0
2872	20			80	0.995493
2889	25			75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain