SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene2468

Length: 1593 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene2468

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Phospholipase D (Fragment) n=2 Tax=Vitis vinifera RepID=D7TW92_VITVI	-	-	0.0	68%
FL-Next	sp=Phospholipase D; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	65%
Sma3	Phospholipase D	-	-	4.78e-33	-

Source	ECs	Term	Type	e value	Identity
Sma3	Phospholipase D.	EC:3.1.4.4	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Glycerophospholipid metabolism	00564	%
Sma3	Ether lipid metabolism	00565	%
Sma3	Metabolic pathways	01100	%

Source	Gene names
Sma3	A_IG005I10.13; At1g52570; At1g55180; At2g42010; At3g15730; At4g00240; At4g11830; At4g11840; At4g11850; At4g35790; At5g25370; F18G18.110; F4B14.60; F5I10.13; F6D8.21; F7A10.25; GSVIVT00000347001; GSVIVT00001073001; GSVIVT00007835001; GSVIVT00012363001; GSV

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	plastid	GO:0009536	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	cytosolic ribosome	GO:0022626	Cellular Component	-
Sma3	clathrin-coated vesicle	GO:0030136	Cellular Component	-
Sma3	cytoplasmic vesicle	GO:0031410	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	phospholipase D activity	GO:0004630	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	NAPE-specific phospholipase D activity	GO:0070290	Molecular Function	-
Sma3	fatty acid metabolic process	GO:0006631	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	phospholipid catabolic process	GO:0009395	Biological Process	-
Sma3	response to cold	GO:0009409	Biological Process	-
Sma3	response to water deprivation	GO:0009414	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	response to abscisic acid stimulus	GO:0009737	Biological Process	-
Sma3	abscisic acid mediated signaling pathway	GO:0009738	Biological Process	-
Sma3	positive regulation of abscisic acid mediated signaling pathway	GO:0009789	Biological Process	-
Sma3	embryo development ending in seed dormancy	GO:0009793	Biological Process	-
Sma3	defense response to bacterium, incompatible interaction	GO:0009816	Biological Process	-
Sma3	seed germination	GO:0009845	Biological Process	-
Sma3	ethylene mediated signaling pathway	GO:0009873	Biological Process	-
Sma3	regulation of stomatal movement	GO:0010119	Biological Process	-
Sma3	programmed cell death	GO:0012501	Biological Process	-
Sma3	lipid catabolic process	GO:0016042	Biological Process	-
Sma3	membrane lipid catabolic process	GO:0046466	Biological Process	-
Sma3	phosphatidylcholine metabolic process	GO:0046470	Biological Process	-
Sma3	phosphatidic acid metabolic process	GO:0046473	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	C2 calcium-dependent membrane targeting	IPR000008	-	-
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Phospholipase D/Transphosphatidylase	IPR001736	-	-
Sma3	YLP motif	IPR004019	-	-
Sma3	Adenosine/AMP deaminase active site	IPR006650	-	-
Sma3	Phospholipase D, plant	IPR011402	-	-
Sma3	Phospholipase D	IPR015679	-	-
Sma3	C2 membrane targeting protein	IPR018029	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G00240.1	PLDBETA2 phospholipase D beta 2 chr4:106380-110718 REVERSE LENGTH=927	68%
RefSeq	Arabidopsis thaliana	NP_567160.1	phospholipase D beta 2 [Arabidopsis thaliana]	68%
RefSeq	Populus trichocarpa	XP_002320087.1	predicted protein [Populus trichocarpa]	67%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQ49

Fln msg: your sequence is shorter than subject: 269 - 861

Fln protein: PMELALKIADKIRAHERFAVYVLIPMWPEGIPTSIAMQRILYWQGQTMQMMYETIFKALEEVGLEKTYHPQDYLNFFCLGNREVRDNNEAPSKSAVPENTPHARALKNRRFMIYVHSKGMIVDDEYVILGSANINQRSMEGSRDTEIAMGAYQPQYTWSKKRDHPHGQVYGYRMALWAEHLAVVEDCFQHPESLECVRRVGALSENYWKQFSANEVTEMKGHLMKYPVMVGANGKVKPLPGYETFPDIGGNVLGTLGTPDFVHENLTI*

Protein Length: 270

Fln nts: TCCAATGGAACTTGCTCTGAAGATAGCTGACAAGATCAGAGCACATGAAAGATTTGCTGTGTATGTTTTGATCCCCATGTGGCCAGAAGGCATTCCAACAAGTATTGCCATGCAGCGGATTCTTTACTGGCAGGGACAGACTATGCAGATGATGTACGAGACTATTTTCAAGGCTTTGGAAGAAGTTGGTCTTGAAAAGACATATCATCCTCAAGATTATTTGAACTTCTTTTGTCTAGGGAATCGTGAAGTAAGAGATAATAACGAAGCACCTTCTAAAAGTGCAGTTCCGGAAAATACACCTCATGCTCGAGCTCTGAAAAATCGCCGTTTCATGATTTATGTCCATTCAAAGGGTATGATTGTAGATGATGAGTATGTCATTCTAGGATCAGCTAATATTAACCAACGTTCAATGGAAGGTTCAAGAGATACAGAGATAGCTATGGGTGCATATCAACCACAATATACATGGTCTAAAAAGCGAGACCATCCACATGGCCAGGTATATGGGTATAGAATGGCTCTCTGGGCAGAGCATCTTGCAGTTGTTGAGGATTGCTTTCAGCATCCAGAAAGTTTGGAATGTGTAAGGAGAGTTGGGGCTCTCTCTGAAAATTACTGGAAGCAGTTCAGTGCCAACGAGGTGACTGAAATGAAGGGTCACCTAATGAAATACCCAGTAATGGTAGGAGCAAACGGAAAAGTGAAACCTCTGCCTGGATATGAAACCTTCCCTGATATCGGGGGAAATGTCCTTGGTACACTTGGGACACCTGACTTTGTCCATGAAAACTTAACTATCTGA___ATTTGTTTTCTTGAAATTGGAGTAGATAACTGGAGCTATTATGGTAATCCATCAAAAGCCAAAACTTGGAATGCATCAAAAATGTTTTGCTCCTTGCTGCACACTCACCTCCATAGACATTTATGTCATATATGTTGTTTCTTGTATGATTTCGTTGGACATTAGATTTGGGATAGCCTGAAGTTTGAACCAATTGCTAGCTAGCTTCTTAGGTCAACTTGCATGGTACATTTGATATGGGCAATAAGCTTCCACATGTGTTATTGGCCCCCTTCCTTAATACAACTATTAATAATCAGCCTTATCCTTAATATGGGGAAACAACTGATAAACTCAAATCTGCTTACACGGTCTCTGAGTTACAGGAAGGATTCCAGTTCTGCTTCATGGCTGGGTTCAGTGGTGTTCTGCATTATTTGGAGATTGCCCTTTTCTAGTTCGAACTTCCTTCTCTGGGACATGGCGGTGTTTGTAGATTTTGACATCATTTATTCTATGGTGATCAGCTGTACAGTGTATGTACATCTTTGAACAGAAGGTTAATTCATTTTCTTCTTTTTGTTCGATGTGGCAGGATGACGGAACAGATGCTCAATTTCTAGATCCCATAACTTATTCTGATTTTATCTCAGCGTTAGTAGCGTTATGGAAGAGGCCGTTTATTGTATCTTTTTCAAAAAAGAAAAAGAGATGTGGAAACAATTTAGAATATTTGAAGGTTTTGTTCTTCTGATTGTGTCACCTCTTTATTTTACAGGAAGCTGACCCAATCTACCTATTTACGT

Fln Alignment:

Contig2468___PMELALKIADKIRAHERFAVYVLIPMWPEGIPTSIAMQRILYWQGQTMQMMYETIFKALEEVGLEKTYHPQDYLNFFCLGNREVRDNNEAPSKSAVPENTPHARALKNRRFMIYVHSKGMIVDDEYVILGSANINQRSMEGSRDTEIAMGAYQPQYTWSKKRDHPHGQVYGYRMALWAEHLAVVEDCFQHPESLECVRRVGALSENYWKQFSANEVTEMKGHLMKYPVMVGANGKVKPLPGYETFPDIGGNVLGTLGT-PD
B8LQ49______________PMEIALKVADKIRKGERFAVYVVIPMWPEGNPTGASVQEILFWQSQTMEMMYGIIAEALKDAGLADSQHPQDYLNFYCLGNREPKDGREPPPTNSPAENSPQGQAQKFRRFMIYVHAKGMIVDDEYVIMGSANINERSLDGSRDTEIAMGSYQPHHTWDRKQRHPHGQVYGYRMSLWAEHLGLLEDTFNNPQSLDCVRRVNELAEKYWQQFTAEEVIKIKGHLLKYPLKVEADGKVVPLPGNESFPDVGGKILGAHGSLPD

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
1131	77		11		0.968132
1132	88			11	0.923564
1135		11	88		0.923564
1140	88			11	0.923564
1141	88		11		0.923564
1142	88		11		0.923564
1145		88		11	0.923564
1146	88	11			0.923564
1148	88	11			0.923564
1193		90		10	0.90298
1197	81		18		0.999994
1201		90		10	0.90298
1205		20	80		0.999996
1211		20	80		0.999996
1213		22	77		0.999999
1214	22		77		0.999999
1227			10	90	0.90298
1229		10		90	0.90298
1233		10	90		0.90298
1234	90	10			0.90298
1238		30	70		1.0
1240		90	10		0.90298
1242	11			88	0.923564
1243		11		88	0.923564

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain