SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene2148

Length: 2728 nt

UniGene Fasta

>sp_v3.0_unigene2148
TGGTAAAAGTTTTTGGCCGTATGACACGGTAAGAAAATGCAAGAAAATGCAGAAAACACAATAACAAGAACCCAGCATACTGCTGAACTTCATTTGACGTTACAGCTCTCACACCCGTGTTCTTCAACACACCACATACCTTAACAAAAAGCATTACTGATTCTAGCACATGATTTGCATGGTGCACTGTTACAAATATTTCTGAGCATAACACGGTTTTACATTCTTAGGGATGGTACATTTTCTGATCCGACACGACTTTTCTGACTTTATATATTAAACCATTGCCCGACTGGCTCACGGGGAGTGAGCAAGCGAGCGAGGGAGCATACACATGCTTTTGAGACTACTTGAATCAGGCTCCTGAATCCCTTTGATTTCTGAATCCCCTTCATTCCTGCCCAGCTGAGAGCTTCATCAGCCATGGAAGTTCTCGGAAGAAAAACACCCACATTGCCATCTTTCATGTTCAACTTCAAATCTAGTGAAAATGATACATCATTTAGATGCCACTGCAGCTTCCCCACTTCCAGCCATGCATATGCAAATGATGCAAACCTGTTTACGACGGGCCATGCAATTTTTCGTCAGAGCTCGAGAGCGGCAAGTAATGCCGTGGTTCGCGGACTGGAAGCAACGGAAATCTCAGAGGACGCCAAAGTTTCAGATTTGAAGGGTTTTGGTGGCCTAGAAGCTGTTCATGAAATAATGTCGACATCGTTGTTGAGGGTTTCCTCGGATTCATTGCAGTACGAGGAAGGGTTTCTGGGCGCCATTTCTGAAAAGACAGCGCCTGGTTCTGAAGGACCAGTTAATGTTCCGACGGCAGTCTCATATTTGACGAGAATTTTATCGTCTAAAGTTTACGAGACCGCCATTGAGTCGCCATTACAGTACGCGCCCAAGCTTTCTGAGAAGACGGGCGTGGAGATTTGGCTCAAACGCGAGGATTTGCAGCCTACTTTTTCATTTAAGTTGCGTGGAGCATACAACATGATGGCCAAGCTACCAAGGGAACAGCTAGAGAGGGGAGTTATTTGCTCTTCTGCTGGTAATCATGCTCAAGGCGTTGCATTGGCAGCTCAAAAACTCTTCTGTAATGCAGTGATAGCAATGCCGGTTACGACCCCTGAAATCAAGTGGAAATCGGTTGAGCAACTTGGTGCAACTGTTGTCCTTGTTGGTGATTCATATGATGAAGCCCAGCTTTATGCTAAGCAACGAGGTGAAGAGGAGGGTCTCACTTTTATACCTCCTTTTGATCATCCAGATATCATTTCAGGCCAGGGAACTGTTGGAATGGAAATTATACGGCAATTTCAAGGTCCATTACATGCTGTATTTGTACCAGTGGGTGGAGGTGGTCTAATTGCTGGCATTGCTGCTTACATAAAGCGAGTCCGGCCTGAGGTACGGATAATTGGTGTGGAGCCAGCAGATGCAAATTCCATGGCTTTATCTTTGTATCATGGGCAGAGAGTGATGTTGGAGAATGTAGGAGGGTTTGCAGATGCTGTAGCAGTTAAAGTTGTAGGAGAAGAAACATTTCGTCTGTGCAGAGAGTTGGTGGATGGAGTGATTCTTGTCAGTCGTGACGCTATATGTGCATCAATAAAGGACATGTTTGAAGAGAAGAGGAGCATTTTAGAACCTGGTGGGGCTTTGGCCTTAGCTGGAGCACAAGCATATTGCAAATATTATGGGTTGAAGGATGAAACCATGGTGGTGATAACTAGTGGGGCAAACATGAATTTTGGACGTCTGCAGCTAGTAACTGAACTTGCTGAAGTTGGTGCCAAAAGAGAAGCTGTTCTTGCAACTTTTCTCCCAGAAGAGTGTGGAAGTTTTAAGCAGTTTTGCCACTTGGTTGGGCCAATGAATATTACAGAATTCAAATATCGATACAATGCAGACAAAGAAGAAGCACTCGTTTTGTATAGTGTTGGTATTCACAAGGGTACAGAACTCAGAGTTATGAAGGATCATATGGAATCTTCTGGTTTAAGAACGCTTGATCTTACAGACAATGATCTGGCCAAAGATCATCTGCGCCATCTTATGGGAGGACGTTCAAATGTTGAAGATGAGCTTCTTTGCAGATTCGTCTTTCCTGAAAGACCGGGTGCGTTGATGAAGTTTTTAGACACATTTAGTCCACGTTGGAATATAAGCTTATTCCATTACCGTGCTCAGGGTCAAATGGGGGCAAATGTGTTGGTTGGCATACAGGTTCCAGAAAAAGACAAAGAAGAGTTTTCAGCACGAGTAAGCACACTTGGATATGAATATGAGGATGAAAGGAAGAATGAAGCTTTCATGCTATTGATGCACTAACTTGGCTTGAAACGGCGAATTTCTTCGTCACTGACTTTTGGTAGAAATGCTAGTTAAGGGTATTGACATGTGTATGATAGATAGGCACTTTCTTGATAAAGTGACTGGGGACTGTCAGCTCATCCATTGTTTGGCTTTCCAAGGAAATGACAATCTGATAATAAAATACATTACCACTGCTATGGGTGAATCCTAGTGTCTTCCCTGGGACTGAAACATTAGTATAAATCCATTTTAACCCCAGATCTATTAAGCAATGGGGAGGAATAAAAATAAATCAAGGCACAAATTTCTCAGTGCTTAAAAGTTCTCCATTTAAGATGGACCTATGCTTGTTTATTTTATTATTACATAAATATTTTTAACTTTAAGGTAATTAATGACCCTATTTTCT

Ace file of the UniGene sp_v3.0_unigene2148

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	putative dehydratase/deaminase [Oryza sativa Japonica Group]	-	-	0.0	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	75%
Sma3	Threonine deaminase	-	-	2.155e-23	-

Source	ECs	Term	Type	e value	Identity
Sma3	Threonine ammonia-lyase.	EC:4.3.1.19	-	7.152e-30	-

Source	KEGGs	Term	e value	Identity
Sma3	Glycine, serine and threonine metabolism	00260	7.152e-30	%
Sma3	Valine, leucine and isoleucine biosynthesis	00290	7.152e-30	%
Sma3	Metabolic pathways	01100	7.152e-30	%
Sma3	Biosynthesis of secondary metabolites	01110	7.152e-30	%

Source	Gene names
Sma3	At3g10050; CHLREDRAFT_196595; GSVIVT00000550001; GSVIVT00021724001; LOC_Os03g50510; MICPUCDRAFT_63278; MICPUN_54333; OMR1; OSJNBa0014G15.29; OSJNBb0033N16.20; OSTLU_39224; Os03g0713000; OsI_13266; OsJ_12332; Ot12g01590; PHATRDRAFT_49047; PHYPADRAFT_199432

Source	GOs	Term	Type	Identity
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	L-threonine ammonia-lyase activity	GO:0004794	Molecular Function	-
Sma3	pyridoxal phosphate binding	GO:0030170	Molecular Function	-
Sma3	response to stress	GO:0006950	Biological Process	-
Sma3	isoleucine biosynthetic process	GO:0009097	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Dynamin central domain	IPR000375	-	-
Sma3	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	IPR000634	-	-
Sma3	Threonine dehydratase, C-terminal	IPR001721	-	-
Sma3	Pyridoxal phosphate-dependent enzyme, beta subunit	IPR001926	-	-
Sma3	Threonine dehydratase I	IPR005787	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G10050.1	OMR1 L-O-methylthreonine resistant 1 chr3:3099164-3101741 REVERSE LENGTH=592	72%
RefSeq	Arabidopsis thaliana	NP_187616.1	threonine dehydratase biosynthetic [Arabidopsis thaliana]	72%
RefSeq	Populus trichocarpa	XP_002326230.1	predicted protein [Populus trichocarpa]	74%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQZ5

Fln msg:

Fln protein: MEVLGRKTPTLPSFMFNFKSSENDTSFRCHCSFPTSSHAYANDANLFTTGHAIFRQSSRAASNAVVRGLEATEISEDAKVSDLKGFGGLEAVHEIMSTSLLRVSSDSLQYEEGFLGAISEKTAPGSEGPVNVPTAVSYLTRILSSKVYETAIESPLQYAPKLSEKTGVEIWLKREDLQPTFSFKLRGAYNMMAKLPREQLERGVICSSAGNHAQGVALAAQKLFCNAVIAMPVTTPEIKWKSVEQLGATVVLVGDSYDEAQLYAKQRGEEEGLTFIPPFDHPDIISGQGTVGMEIIRQFQGPLHAVFVPVGGGGLIAGIAAYIKRVRPEVRIIGVEPADANSMALSLYHGQRVMLENVGGFADAVAVKVVGEETFRLCRELVDGVILVSRDAICASIKDMFEEKRSILEPGGALALAGAQAYCKYYGLKDETMVVITSGANMNFGRLQLVTELAEVGAKREAVLATFLPEECGSFKQFCHLVGPMNITEFKYRYNADKEEALVLYSVGIHKGTELRVMKDHMESSGLRTLDLTDNDLAKDHLRHLMGGRSNVEDELLCRFVFPERPGALMKFLDTFSPRWNISLFHYRAQGQMGANVLVGIQVPEKDKEEFSARVSTLGYEYEDERKNEAFMLLMH*

Protein Length: 638

Fln nts: TGGTAAAAGTTTTTGGCCGTATGACACGGTAAGAAAATGCAAGAAAATGCAGAAAACACAATAACAAGAACCCAGCATACTGCTGAACTTCATTTGACGTTACAGCTCTCACACCCGTGTTCTTCAACACACCACATACCTTAACAAAAAGCATTACTGATTCTAGCACATGATTTGCATGGTGCACTGTTACAAATATTTCTGAGCATAACACGGTTTTACATTCTTAGGGATGGTACATTTTCTGATCCGACACGACTTTTCTGACTTTATATATTAAACCATTGCCCGACTGGCTCACGGGGAGTGAGCAAGCGAGCGAGGGAGCATACACATGCTTTTGAGACTACTTGAATCAGGCTCCTGAATCCCTTTGATTTCTGAATCCCCTTCATTCCTGCCCAGCTGAGAGCTTCATCAGCC_-_ATGGAAGTTCTCGGAAGAAAAACACCCACATTGCCATCTTTCATGTTCAACTTCAAATCTAGTGAAAATGATACATCATTTAGATGCCACTGCAGCTTCCCCACTTCCAGCCATGCATATGCAAATGATGCAAACCTGTTTACGACGGGCCATGCAATTTTTCGTCAGAGCTCGAGAGCGGCAAGTAATGCCGTGGTTCGCGGACTGGAAGCAACGGAAATCTCAGAGGACGCCAAAGTTTCAGATTTGAAGGGTTTTGGTGGCCTAGAAGCTGTTCATGAAATAATGTCGACATCGTTGTTGAGGGTTTCCTCGGATTCATTGCAGTACGAGGAAGGGTTTCTGGGCGCCATTTCTGAAAAGACAGCGCCTGGTTCTGAAGGACCAGTTAATGTTCCGACGGCAGTCTCATATTTGACGAGAATTTTATCGTCTAAAGTTTACGAGACCGCCATTGAGTCGCCATTACAGTACGCGCCCAAGCTTTCTGAGAAGACGGGCGTGGAGATTTGGCTCAAACGCGAGGATTTGCAGCCTACTTTTTCATTTAAGTTGCGTGGAGCATACAACATGATGGCCAAGCTACCAAGGGAACAGCTAGAGAGGGGAGTTATTTGCTCTTCTGCTGGTAATCATGCTCAAGGCGTTGCATTGGCAGCTCAAAAACTCTTCTGTAATGCAGTGATAGCAATGCCGGTTACGACCCCTGAAATCAAGTGGAAATCGGTTGAGCAACTTGGTGCAACTGTTGTCCTTGTTGGTGATTCATATGATGAAGCCCAGCTTTATGCTAAGCAACGAGGTGAAGAGGAGGGTCTCACTTTTATACCTCCTTTTGATCATCCAGATATCATTTCAGGCCAGGGAACTGTTGGAATGGAAATTATACGGCAATTTCAAGGTCCATTACATGCTGTATTTGTACCAGTGGGTGGAGGTGGTCTAATTGCTGGCATTGCTGCTTACATAAAGCGAGTCCGGCCTGAGGTACGGATAATTGGTGTGGAGCCAGCAGATGCAAATTCCATGGCTTTATCTTTGTATCATGGGCAGAGAGTGATGTTGGAGAATGTAGGAGGGTTTGCAGATGCTGTAGCAGTTAAAGTTGTAGGAGAAGAAACATTTCGTCTGTGCAGAGAGTTGGTGGATGGAGTGATTCTTGTCAGTCGTGACGCTATATGTGCATCAATAAAGGACATGTTTGAAGAGAAGAGGAGCATTTTAGAACCTGGTGGGGCTTTGGCCTTAGCTGGAGCACAAGCATATTGCAAATATTATGGGTTGAAGGATGAAACCATGGTGGTGATAACTAGTGGGGCAAACATGAATTTTGGACGTCTGCAGCTAGTAACTGAACTTGCTGAAGTTGGTGCCAAAAGAGAAGCTGTTCTTGCAACTTTTCTCCCAGAAGAGTGTGGAAGTTTTAAGCAGTTTTGCCACTTGGTTGGGCCAATGAATATTACAGAATTCAAATATCGATACAATGCAGACAAAGAAGAAGCACTCGTTTTGTATAGTGTTGGTATTCACAAGGGTACAGAACTCAGAGTTATGAAGGATCATATGGAATCTTCTGGTTTAAGAACGCTTGATCTTACAGACAATGATCTGGCCAAAGATCATCTGCGCCATCTTATGGGAGGACGTTCAAATGTTGAAGATGAGCTTCTTTGCAGATTCGTCTTTCCTGAAAGACCGGGTGCGTTGATGAAGTTTTTAGACACATTTAGTCCACGTTGGAATATAAGCTTATTCCATTACCGTGCTCAGGGTCAAATGGGGGCAAATGTGTTGGTTGGCATACAGGTTCCAGAAAAAGACAAAGAAGAGTTTTCAGCACGAGTAAGCACACTTGGATATGAATATGAGGATGAAAGGAAGAATGAAGCTTTCATGCTATTGATGCACTAA___CTTGGCTTGAAACGGCGAATTTCTTCGTCACTGACTTTTGGTAGAAATGCTAGTTAAGGGTATTGACATGTGTATGATAGATAGGCACTTTCTTGATAAAGTGACTGGGGACTGTCAGCTCATCCATTGTTTGGCTTTCCAAGGAAATGACAATCTGATAATAAAATACATTACCACTGCTATGGGTGAATCCTAGTGTCTTCCCTGGGACTGAAACATTAGTATAAATCCATTTTAACCCCAGATCTATTAAGCAATGGGGAGGAATAAAAATAAATCAAGGCACAAATTTCTCAGTGCTTAAAAGTTCTCCATTTAAGATGGACCTATGCTTGTTTATTTTATTATTACATAAATATTTTTAACTTTAAGGTAATTAATGACCCTATTTTCT

Fln Alignment:

Contig2148___ANLFTTGHAIFRQSSRAASNAVVRGLEATEISEDAKVSDLKGFGGLEAVHEIMSTS-------LLRVSSDSLQYEEGFLGAISEKTA-PGSEGPVNV-PTAVSYLTRILSSKVYETAIESPLQYAPKLSEKTGVEIWLKREDLQPTFSFKLRGAYNMMAKLPREQLERGVICSSAGNHAQGVALAAQKLFCNAVIAMPVTTPEIKWKSVEQLGATVVLVGDSYDEAQLYAKQRGEEEGLTFIPPFDHPDIISGQGTVGMEIIRQFQGPLHAVFVPVXXXXXXXXXXXXXKRVRPEVRIIGVEPADANSMALSLYHGQRVMLENVGGFADAVAVKVVGEETFRLCRELVDGVILVSRDAICASIKDMFEEKRSILEPXXXXXXXXXXXYCKYYGLKDETMVVITSGANMNFGRLQLVTELAEVGAKREAVLATFLPEECGSFKQFCHLVGPMNITEFKYRYNADKEEALVLYSVGIHKGTELRVMKDHMESSGLRTLDLTDNDLAKDHLRHLMGGRSNVEDELLCRFVFPERPGALMKFLDTFSPRWNISLFHYRAQGQMGANVLVGIQVPEKDKEEFSARVSTLGYEYEDERKNEAFMLLMH
B8LQZ5______________ASTATPESRAFQHDTNVPSNPT--GIPNRRLEEIRAVADGVGIGFVDKTSESATPTSSRDPLPLLKVSSESLQYESGYLGAISDKTSDPHSDTNGNGGPTNVSYLTSILSSKVYEVAIESPLQYAPKISQRSGVRILLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAERLKCNAVIVMPVTTPEIKWKSVERLGATVVLIGDSYDEAQTYAKRRSEEEGRIFIPPFDHPDVIIGQGTIGMEIVHQNPGPLHAVFVPVGGGGLIAGIAAYMKQVRPEVRIIGVEPADANAMALSLYRGQRVMLDQVGGFADGVAVKVVGEETFRLCRELVDGIVLVSRDAICASIKDMFEEKRSILEPAGALALAGANAYCKYYGLKDETVVAITSGANMNFERLRLVTQLADVGAKREAVLATFLQEEHGSFKNFCQLVGPINITEFKYRYDIGKDKALVLYSVGVHKAEELIAMKDRLESSGLRTLDLTDNDLAKDHLRHLMGGRSNLENELLYRFVFPERPGALMKFLDAFSPRWNISLFHYRAQGDTGANVLVGIQVPKEDEEEFIGQAKTLGYEFKDERKNKAYRLLMH

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
506	16		83		0.994512
512	83			16	0.994512
540	83		16		0.994512
649	16		83		0.994512
667	20		80		0.995493
796		33	66		0.997452
797	33		66		0.997452
2249	85		14		0.993533
2269	87		12		0.992554
2274	87		12		0.992554
2593		20		80	0.995493
2594	20		80		0.995493
2595			80	20	0.995493
2596			75	25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain