SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene1903

Length: 1823 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene1903

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	putative phragmoplast-associated kinesin-related protein [Arabidopsis thaliana] gb\|AAM74514.1\| AT3g23670/MDB19_16 [Arabidopsis thaliana] gb\|AAN16470.1\| phragmoplast-associated kinesin-related protein 1-like protein [Arabidopsis thaliana] gb\|AAN16471.1\| ph	-	-	0.0	44%
FL-Next	sp=Putative WEB family protein At1g65010, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	21%
Sma3	Phragmoplast-associated kinesin-related protein 1	-	-	5.167e-09	-

Source	Gene names
Sma3	AT4g14150; At3g23670; At4g14150; GSVIVT00031645001; OSIGBa0144C23.2; OSJNBa0038P21.12; OSJNBa0048K16.12; Os04g0350300; OsI_07265; OsI_15450; OsI_15463; OsJ_06785; OsJ_14379; PAKRP1; PAKRP1L; POPTRDRAFT_572919; POPTRDRAFT_756178; RCOM_1645450; SIP18; SIP27

Source	GOs	Term	Type	Identity
Sma3	microtubule associated complex	GO:0005875	Cellular Component	-
Sma3	phragmoplast	GO:0009524	Cellular Component	-
Sma3	microtubule motor activity	GO:0003777	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	plus-end-directed microtubule motor activity	GO:0008574	Molecular Function	-
Sma3	phragmoplast assembly	GO:0000914	Biological Process	-
Sma3	microtubule-based movement	GO:0007018	Biological Process	-
Sma3	actin filament polymerization	GO:0030041	Biological Process	-
Sma3	microgametogenesis	GO:0055046	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Kinesin, motor domain	IPR001752	-	-
Sma3	ARP23 complex 20kDa subunit	IPR008384	-	-
Sma3	Kinesin-related conserved domain	IPR010544	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G23670.1	PAKRP1L, KINESIN-12B phragmoplast-associated kinesin-related protein, putative chr3:8519290-8525055 FORWARD LENGTH=1313	45%
RefSeq	Arabidopsis thaliana	NP_189009.2	putative phragmoplast-associated kinesin-related protein [Arabidopsis thaliana]	45%
RefSeq	Populus trichocarpa	XP_002303008.1	predicted protein [Populus trichocarpa]	47%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: sp_plants

Fln subject: F4I8B9

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 750 aas, atg_distance in limit (1-15): atg_distance = 14, No stop codon before M and low homology subject, your sequence is shorter than subject: 509 - 1345

Fln protein: MEDKSQWQLMPLEKSRGSKGPRHELSQAVEGVLAGSIRREMALEEVCNKQATEIEHLNRLVRQYKHERECNSIIQQAREEKISRLEALVDGTISSEEYLKDELALLAFEHKLLEDKFQNHPEVTRAMIEEKRLSEELEKYRSFCELGERDALIEEIKNLRTQLQYYLDLNSSVSAKKLRLSVVNQSTQRPEFNKSAHSLGLCTISELPVAAPDETRDTLSDKKWEEENRQWTDRENEWISLVDELRAELDSVNCLAEKRKQEVENEQRCSEELKEALQMAMEGHARILDQYAELQEKYMSLRAKHRKIREGVANARQAAVMAGIRGAALSLIESQAAELAAMKLDNEQECEALKDEIDGLQAQLRDTVEAVQAAGELVVRLKEAEEAVSIVQDAASRVDQDAEALRRDMQKLKRRHATEIATLNQRLLESRLQKSSVCPMCQVAERVKFEFPDADNATLEAASINSSEAQEEWREELQQQFLPEFQEHDELLRADQPSWFSGHDKCNI*

Protein Length: 510

Fln nts: TGGAAGACTTGATATAATTGAGGCAAATTCCTTCAAGGACAGAGAAGAC_-_ATGGAAGATAAGTCCCAATGGCAGTTAATGCCTCTTGAAAAATCAAGAGGTTCAAAAGGACCTAGGCATGAGCTTAGTCAAGCAGTGGAGGGAGTTTTAGCAGGGTCCATTAGACGAGAAATGGCCCTGGAAGAGGTTTGCAACAAGCAAGCGACAGAAATTGAGCACCTTAACCGTTTGGTTAGACAATACAAACATGAACGAGAATGTAATTCCATCATACAGCAAGCTAGAGAGGAAAAAATTTCCCGTCTTGAAGCTCTTGTGGATGGTACCATTTCAAGTGAGGAATATCTGAAAGATGAACTGGCATTACTGGCTTTTGAACACAAGTTATTGGAAGACAAATTCCAGAATCATCCAGAAGTGACACGAGCAATGATTGAGGAAAAACGTCTTTCAGAAGAACTAGAGAAGTACCGTAGCTTCTGTGAATTGGGGGAAAGGGATGCATTAATCGAAGAGATAAAGAATCTGCGAACTCAGTTGCAATATTATTTAGATTTGAACAGTTCTGTATCTGCCAAGAAGTTGCGTCTGTCTGTTGTCAACCAATCTACTCAGAGGCCTGAATTTAACAAATCTGCTCATTCCCTGGGTCTCTGTACGATCTCTGAGTTGCCTGTTGCAGCTCCTGATGAAACCAGAGACACTTTGTCAGACAAGAAATGGGAGGAGGAGAATCGGCAGTGGACAGATAGGGAAAACGAATGGATCTCTTTGGTTGATGAATTGAGAGCAGAGCTTGATTCTGTCAATTGCTTGGCAGAGAAAAGAAAGCAAGAAGTGGAAAATGAGCAAAGATGTTCTGAAGAGCTGAAAGAAGCACTCCAGATGGCTATGGAGGGTCATGCACGCATTCTAGATCAATATGCAGAGCTTCAAGAAAAATACATGAGCTTGCGTGCCAAGCATCGAAAGATTAGAGAAGGTGTAGCAAATGCCAGACAGGCTGCTGTAATGGCGGGCATTAGGGGTGCTGCATTGTCCTTAATTGAATCTCAGGCAGCAGAACTAGCAGCCATGAAGCTTGATAATGAACAAGAATGTGAAGCCCTGAAAGATGAGATAGATGGGTTACAAGCCCAATTAAGGGACACTGTAGAAGCAGTTCAAGCAGCTGGGGAGCTGGTGGTCCGGTTGAAAGAGGCAGAAGAAGCTGTATCAATTGTTCAAGATGCTGCTTCCCGTGTTGATCAAGATGCAGAAGCACTGCGTCGGGACATGCAAAAATTGAAGCGGAGACATGCCACTGAGATTGCCACATTGAACCAGCGGCTTCTAGAGAGTCGCCTGCAAAAATCATCTGTGTGCCCAATGTGCCAAGTTGCAGAACGAGTAAAATTTGAATTTCCAGATGCAGACAATGCCACTCTCGAGGCAGCATCTATCAACAGTTCTGAAGCGCAAGAAGAATGGAGAGAAGAATTGCAGCAACAATTTCTCCCTGAATTTCAGGAACATGATGAGCTATTAAGAGCAGATCAACCTTCATGGTTTAGTGGACACGATAAGTGCAACATATGAAAATATTCCTCCTCTACCTATTCTTCTGAGCAGGTGGTATACAGATAGGTAAGTGTTTGTCAGAAGATGTACAGTAGATAGTTGAAACTCTCTTATTCATTAATTCATTCTTTGGCATATTCTACTCTGTTCTCTACCAGAACTGGAACATGGGTTATCAAATTTTTTATCAATTATGGAAGGAGAGATGTTATCAATGGCTATGGCCACAGCCCAATTTATGGGTGAATTTTGAAGTTTACTATTG

Fln Alignment:

Contig1903___QATEIE-HLNRLVRQYKHERECNSIIQQAREEKISRLEALVDGTISSEEYLKDELALLAFEHKLLEDKFQNHPEVTRAMIEEKRLSE-----ELEKYRSFCELGERDALIEEIKNLRTQLQYYLDLNSSVSAKKLRLSVVNQSTQRPEFNKSAHSLGLCTISELPVAAPDETRDTLSDKKWEEENRQWTDRENEWISLVDELRAELDSVNCLAEKRKQEVENEQRCSEELKEALQMAMEGHARILDQYAELQEKYMSLRAKHRKIREGVANARQAAVMAGIRGAALSLIESQAAELAAMKLDNEQECEALKDEIDGLQAQLRDTVEAVQAAGELVVRLKEAEEAVSIVQDAASRVDQDAEALRRDMQ-----KLKRRHATEIATLN-QRLLESRLQKSSVCPMCQVAERVKFEFPDADNATLEAASINSSEAQ
F4I8B9______________KGTELQTQLNQIQEDLKKADEQIELLKKDKAKAIDDLKESEKLVEEANEKLKEALAAQKRAEESFEVEKFRAVELEQAGLEAVQKKDVTSKNELESIRSQHAL-DISALLSTTEELQRVKH---ELSMTADAKNKALSHAEEATKIAEIHAEKAEILASELGRLKA---------LLGSKEEKEAIEGNEIVSKLKSEIELLRGELEKVSILESSLKEQ----EGLVEQLKVDLEAAKMAESCTNSSVEEWKNKVHELEKEVEESNRSKSSASES----------MESVMKQLAELNHVLHETKSDNAAQKEKIELLEK----TIEAQRTD------LEEYGRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEKAATSNIQNLLDQRTELSIELERCKVEEEKSKKDMESLTLALQEASTESSEAK

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
1171			25	75	0.996473
1175			66	33	0.997452
1186		33		66	0.997452
1329			10	90	0.90298
1332			10	90	0.90298
1333	90			10	0.90298
1337	90	10			0.90298
1338		10	90		0.90298
1340		90		10	0.90298
1342		90	10		0.90298
1348	90			10	0.90298
1349	90	10			0.90298
1351	90	10			0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain