SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene1807

Length: 1343 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene1807

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	papain-like cysteine peptidase XBCP3 [Arabidopsis thaliana]	-	-	1.0e-36	49%
FL-Next	tr=Pseudotzain; Pseudotsuga menziesii (Douglas-fir) (Abies menziesii).	-	-	0.0	47%
Sma3	Cysteine proteinase	-	-	4.606e-19	-

Source	ECs	Term	Type	e value	Identity
Sma3	Hydrolases, Acting on peptide bonds (peptide hydrolases), Cysteine endopeptidases.	EC:3.4.22.-	-	7.054e-10	-

Source	Gene names
Sma3	AT1G47128; AT4g23520; At1g09850; At1g47128; At3g19390; At3g19400; At3g19400/MLD14.12; At5g43060; At5g43060/MMG4.7; BoCP3; BrCP3; C14; CEP1; CHLREDRAFT_206048; CP1; CP2; CP3; CP9; CPRF; CPRZ; CYP1; DC-CP2; DCCP1; DcCysP1; DcCysP4; DcCysP5; DcCysP6; F16G20.

Source	GOs	Term	Type	Identity
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	cysteine-type endopeptidase activity	GO:0004197	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	proteolysis	GO:0006508	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Anaphylatoxin/fibulin	IPR000020	-	-
Sma3	Granulin	IPR000118	-	-
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Peptidase C1A, papain C-terminal	IPR000668	-	-
Sma3	Peptidase M14, carboxypeptidase A	IPR000834	-	-
Sma3	Mannose-binding lectin	IPR001229	-	-
Sma3	Ribosomal protein L5	IPR002132	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	Phospholipase A2, active site	IPR013090	-	-
Sma3	Peptidase C1A, papain	IPR013128	-	-
Sma3	Proteinase inhibitor I29, cathepsin propeptide	IPR013201	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-
Sma3		IPR018069	-	-
Sma3	Heat shock protein DnaJ, conserved site	IPR018253	-	-
Sma3	Ribosomal protein L29, conserved site	IPR018254	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT1G09850.1	XBCP3 xylem bark cysteine peptidase 3 chr1:3201848-3203875 FORWARD LENGTH=437	1.00053e-42	56%
RefSeq	Arabidopsis thaliana	NP_563855.1	xylem bark cysteine peptidase 3 [Arabidopsis thaliana]	1.00053e-42	56%
RefSeq	Populus trichocarpa	XP_002307688.1	predicted protein [Populus trichocarpa]	1.4013e-45	58%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: Q40922

Fln msg: Separated hits, possible frame ERROR between 288 and 290, Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 239 - 454

Fln protein: VSL*VWAFLVVKEHSSYILE*VLMLNLLK*I*SIVKCDS*MVRYGLF*GFNGYEMVVYLIVLRRKK*FSPL*QGIFNGPCSNSLDHAALIVGYGSxxNGLDYWIVKNSWGKSWGMNGYAYMVRNRGDPEGICGINTLASYPIKSSPNPPPPPPIPTPVKCDALWYCPAGSTCCCTLYIFRFCINWGCCHLESAVCCKDQKYCCPQDHPICDSVTAQCLKAARNMTSVTGLAQMSASIL*

Protein Length: 240

Fln nts: GTCAGCCTGTAAGTGTGGGCATTTCTGGTGGTGAAAGAGCATTCCAGTTATATTCTGGAGTAAGTGTTGATGCTAAACCTGTTGAAATGAATTTAAAGCATTGTTAAATGTGATTCTTGAATGGTTCGTTATGGGTTGTTTTGAGGTTTTAATGGGTATGAGATGGTGGTATACTTAATTGTGCTCAGAAGGAAAAAGTAATTTTCCCCCCTCTAACAGGGAATCTTCAATGGTCCGTGCTCAAATTCTTTAGACCATGCTGCTTTGATAGTTGGATATGGCTCAAAxxxCAATGGGCTTGATTACTGGATAGTAAAAAATTCATGGGGAAAAAGTTGGGGCATGAATGGCTATGCATACATGGTCCGAAACAGAGGTGATCCTGAGGGTATTTGTGGAATCAACACATTAGCATCCTATCCCATTAAGTCAAGCCCAAACCCTCCACCACCCCCGCCTATCCCCACTCCAGTTAAATGTGATGCACTCTGGTACTGTCCAGCTGGAAGCACATGTTGTTGTACATTGTACATATTCAGATTCTGTATAAATTGGGGTTGTTGTCATTTGGAATCTGCGGTGTGCTGCAAAGATCAAAAGTATTGCTGTCCTCAAGATCATCCCATATGTGACTCAGTTACTGCCCAGTGTCTCAAGGCTGCAAGAAATATGACAAGTGTAACAGGCCTTGCTCAAATGAGTGCTTCAATTCTATGATACTTTATGGGTGAATATATTCTTCTACTAAGAGCTTTAATGAAGCCTGCAAGATGGAGCTTAACTTCTATTGAGATTACGGTTATGTTCAAACCCCAAATGAAAGATTGCACACAAGCAATGTACTGTTTGTGCTCAAACAGAGAAAAGATGTATTATCTGAGGCTGATATGAGACTTGTTAGTCATTGTTTCATTGGACTTTCAAGATCTGTATGTTAAAAGTTGGACAACTCTTTGATTGTGCGAGCCTGTATGAAACTTGCTAGTCTTTGTCCAGGGAAGATACATGTCGAGACCTATATGTATTTTTGAGAAATACTGCTTGTAGAAGGTTATAGCTGTTCCTTTGGCTAAGCTACTACACTATGAGGGGTGTATCAATGCTGTCAAAATCATTTAGGACTTGCACCAGGCCTTATCCATGCCCATTAGGGGTATTTTGTACCCAGGTGTTTGTGCTTTATAAGCCTGATCACCTTGCCGCTAGACCAGTTCCCATGGACCAAGAACTATGTCTTAAGGTTGTTTAATCCTTTTCTAATGCAGACGTTGGGGCTACTGTGAAGGGTACTTCTATATTTGAATAATTTGGAAATAATGCATAAAGTTCATTGTTTAGCGTATTG

Fln Alignment:

Contig1807___GIFNGPCSNSLDHAALIVGYGSKxNGLDYWIVKNSWGKSWGMNGYAYMVRN-RGDPEGICGINTLASYXXXXXXXXXXXXXXXXX-----VKCDALWYCPAGSTCCCTLYIFRFCINWGCCHLESAVCCKDQKYCCPQDHPICDSVTAQCLKAARN
Q40922______________GVFTSNCGTQLDHGVTLVGYGSExSGIDYWLVKNSWGNSWGEKGFIKLQRNLEGASTGMCGIAMEASYPVKKGANPPNPGPSPPSPVKPPTVCDNYYSCPESNTCCCMYDFGGYCYAWGCCPLNSATCCDDHYSCCPSDHPVCDLDAQTCLKSRKD

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
414		83		16	0.994512
591	50		50		1.0
613	28		71		1.0
667		60		40	1.0
852		63		36	1.0
864	90		10		0.90298
882		15		84	0.999986
884	83			16	0.999991
885		16		83	0.999991
886	83		16		0.999991
891	83	16			0.999991
896			16	83	0.999991
897		16		83	0.999991
898	83			16	0.999991
949		81		18	0.999994
1151	25		75		0.996473
1156	16			83	0.994512
1157	16			83	0.994512
1158	16			83	0.994512
1159	16			83	0.994512
1160	16		83		0.994512
1227	80		20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain