SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene1720

Length: 1740 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene1720

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam02544, Steroid_dh, 3-oxo-5-alpha-steroid 4-dehydrogenase	-	-	1.0e-23	34%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	42%
Sma3	Dfg10 protein, putative	-	-	1.426e-09	-

Source	Gene names
Sma3	At2g16530; F28P22.22; GSVIVT00035975001; GSVIVT00036815001; OSJNBa0020J04.7; Os04g0576800; Os07g0493400; OsI_17065; OsI_26070; OsJ_15866; OsJ_24314; P0038F10.126; POPTRDRAFT_557164; POPTRDRAFT_759753; RCOM_0292280; RCOM_1450960; VITISV_004839;

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	oxidoreductase activity, acting on the CH-CH group of donors	GO:0016627	Molecular Function	-
Sma3	lipid metabolic process	GO:0006629	Biological Process	-

Source	InterPros	Term	Type	e value	Identity
Sma3	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	IPR001104	-	0.0	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G16530.1	3-oxo-5-alpha-steroid 4-dehydrogenase family protein chr2:7163276-7165064 REVERSE LENGTH=343	50%
RefSeq	Arabidopsis thaliana	NP_565389.1	putative polyprenol reductase 2 [Arabidopsis thaliana]	50%
RefSeq	Populus trichocarpa	XP_002313005.1	predicted protein [Populus trichocarpa]	54%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWW4

Fln msg: your sequence is longer than subject: 352 - 260

Fln protein: MLLTASLWLLEHLTILLRVSWLVGIGAILIASIPFRPFASFHRLIMGFARRGKLMNYSSASRPRFSVPQSYFLHFYLVGVIWTMFLLVCTLFFACTTTLPLSSESLRYSSVASQLAGTSDSLSFAKPFSTLVKHSQQAWKTVFVLLLMEAQVLRRLYETVYVFNYSSSARMHVLGYLCGFFFYLAAPLSMCSLCVPEVSSYIITQAVLSVLRQRARIADIELKFLEYGNSLLKLGWWQWTGALVFMWGWIHQLRCHKILGSLRTGNQEETNEYAIPYGDWFEIVSCPHYLAEIVIYAGILIATGASDLTVWLLFGYVVANLTFAATEMHKWYLHKFDSYPRSRWSIIPFVC*

Protein Length: 353

Fln nts: TCAAAGATGAAACGACATGTACGCAGTGACGCGTGTATGCCTCCACTTCTCGTCTCATAAGCATAAGATGGCACCCAATGAGCCAACCGCATATGCTGTGGGCATCTTGAGAATGCTACTACACACGCAAGGAACATCAAATCTCAGATAGACAACTCTTGAGAAATAACCTACTAGGGAAGATACCAAGATTTCCTCGCGGTTTTCAATAGTAAGGAAGCCATATATGTCGGAATGAGTACATCCACCCCATGATGTTGATGTTTCTACCCACTTGAAGGTGACCATTCAATTTCAAGTTCAAGTTAAGAAA_-_ATGCTACTCACAGCTTCTTTGTGGCTACTGGAACACTTAACAATCTTGTTGAGGGTCTCATGGCTTGTGGGCATTGGCGCAATTCTCATTGCGTCCATTCCTTTTCGCCCATTCGCCTCATTCCACAGGCTGATCATGGGTTTCGCAAGAAGAGGGAAACTCATGAATTACTCCTCCGCTTCCAGACCTAGATTCAGTGTGCCTCAGAGCTACTTCCTTCATTTCTACTTGGTGGGTGTGATTTGGACAATGTTTCTACTGGTGTGCACATTATTCTTTGCTTGCACCACAACTCTACCACTTTCATCGGAGTCCCTGAGGTATTCAAGTGTTGCCAGTCAGTTGGCAGGAACTTCAGACTCACTATCGTTTGCAAAACCTTTTTCCACTCTGGTAAAGCATAGTCAGCAAGCATGGAAGACCGTGTTCGTGCTGTTGTTGATGGAGGCACAAGTTCTGAGACGCCTGTATGAGACCGTGTATGTGTTCAATTATAGTTCTTCTGCCCGGATGCACGTTTTAGGGTATCTCTGTGGATTCTTTTTCTACCTGGCAGCACCCTTATCAATGTGCAGTTTGTGTGTCCCAGAGGTGTCCTCTTATATTATCACCCAAGCAGTTCTTTCTGTATTGAGACAAAGAGCTCGTATAGCTGACATAGAGTTGAAATTTTTAGAGTATGGAAATTCTCTTTTGAAACTGGGATGGTGGCAATGGACTGGTGCACTTGTCTTCATGTGGGGATGGATTCATCAACTCCGGTGCCATAAGATACTGGGATCTTTACGAACTGGAAATCAAGAAGAGACAAATGAATATGCTATTCCATATGGCGATTGGTTCGAGATTGTATCTTGCCCCCACTATTTGGCTGAGATTGTCATCTACGCAGGGATTTTAATTGCTACTGGAGCTTCTGATCTCACAGTGTGGCTTCTTTTTGGATATGTGGTTGCCAACCTCACTTTTGCTGCCACTGAGATGCACAAATGGTATCTTCATAAATTTGATAGCTACCCTCGTTCTCGGTGGTCCATCATTCCTTTTGTTTGTTAA___TATTCTTTTGTAATTTTCATAATTCCAGCCATCAATCCTACAGCTTGTTATACCAAGTGCAGATTACTGTTGAAAGGTACAAATAAACGTGACTGCATATTGACATGTATGCAGAAATACTTATCCATTCCATTCTCTGTATGCAATTTGTTTCAATAAGAATTGCAACAATTTAATGCTGCTTATTATGTTCTGCATTGTTTCCAGCCAAAACTCCTGTAAATCAAACTGGTGTTAGTGACATAACTTCCTAATGAAAGTTAAGACATTCGAAGGTTTTTTTATAGAGAACTAATTATGCAAAGGGAATGCTGGCCTACATTGTGTCAATGCAAGATACATGTTTTATATAGGATTAAAAATATTGGACA

Fln Alignment:

Contig1720___GALVFMWGWIHQLRCHKILGSLRTGNQEETNEYAIPYGDWFEIVSCPHYLAEIVIYAGILIATGASDLTVWLLFGYVVANLTFAATEMHKWYLHKF-DSYPRSRWSIIPFV
A9NWW4______________GLLVFITGMIINLWADSVLLSLRRENEG----YKIPRGGLFEYVSCPNYFGEIVEWLGWALMTWS--LAGLSFFLYTAANLGPRAAVHHDWYHEQFPERYPKSRKALIPFV

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
111	25		75		0.996473
112	75			25	0.996473
113	75		25		0.996473
610	66		33		0.997452
1276		76	4	19	1.0
1538	20	80			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain