SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene1640

Length: 2550 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene1640
TGGACTATAGAGTATGGACGGAAGATTGTGTTTGGGTTTATAGAATTCATTACAAATTCGGATTGGTTCTGAAGAATTTATTAGAAGCAATGGGTAATTGGCGGCGGAGAACGCGTGAAATTGTTGGAGTTTTCGTCGGGTTTGACTAAATCTGGTTCAGCAAAGAAGTGCGTGGGTATATTGGCGTTATAGGGTTGAAGGGATTTATGAACAATCAAGGTAGTTCGGTGCAGAGAATGCAAGAAGTAATAGATGTTTGTTTGTTTCTGAATAAATTTGTTGGTGGGTTTGTTGAGAATTGAGTTAGTGAAGGATAATGGATTTGATATGGTGTGAATTCTGATTCTGGGGTTTAAGGTGCTTGCCATTACAGATGAATTGGATGTGATGGGACACGAGACGTGTTTGCAGGTTTTCAGGCTTGATTGAGTCATGGGACAAACAATGGAAGATTCAGAGAGAACTGCAGTGCAAGCTGTGGAGGCTCTGGGGCGAGGCTTTGATTTCACAAATGATTTCAGGCTTAGGTTCTGCAAGGGCAATTCAAGGAGTCGACTGGTAGTTTTGGAGGAGGAGCGAAAGCAAGATATTGTGATTCCTGGAGGCCCTTTGATACGAAATGTGTCGACAGACATTAAGTGTGACAAGGGAGAACGTACCAGATTTAAGTCTGATATTTTGGAATTCAATCAGATGTCTGAGCTATTCAATCAGAGGTCCTCTCTACAAGGGAAGATCCCCTCTGGCAACTTCAATTCTCTGTTTGATTTCAGTGGGTCGTGGTTGCAAGATGCTACAGAAACCAAACACCTGGCCTTTGATGGCTTCTTTATTTCCCTATATCATCTACACCTTACAAAGACGCCACTCATCCTCAAGGAAGGCGTCAAATCGGCCGTTCCTTCTAATTGGGATCCTGCGGCACTTGCCAGGTTTATTGAAACTTTTGGGACACACATTATTGTGTCCGTGGCAATTGGAGGTCAGGATCTGGTGTTTGTAAGGCAGAATCATTCCTCCAGTATTTCACCTGCTGAACTTAAAAATCATCTGGAAAACCTGGGAGATCAAATATTTTCAGATGGCCGTAGCCTTTCGCCCTTCCGTAATAATAAGAGAGAAAGCAGACAGAAGGTTCCAGAGGTTTTTAGCAACATTTTACGATCTACAAATCCTGTGCATGTGGGCAGCTTTTCTTCAACGTCCAGCAAGGATGGATTGACAATCTTTTGCTCTAAAAGGGGTGGAATTATAGATTTACGCAATCACCGTGAGTGGCTTCATACTGTATCCGAGCATCCAGATGCAATACTTTTCTCACTAGTTCCAATTACATCCCTTTTGACAGGGGTTGTTGGCAGCGGTTTCTTAAGTCATGCTATCAATCTATACCTTCGTTACAAACCAGCCATAGATGAATTGCAATATTTCTTGGAATTCCAAGTTCCTCGGCAATGGGCCCCAGTTTACAATGAGTTACCACTTGGGCCTTCTACAAAGAGAATATTCTGTCCTTCATTACAGTTCAGCTTTATGGGCCCCAAACTTTATGTTCGCACAATTCAGGTTTGTGTGAGTAGAAGACCTGTAATTGGGTTACGGTTGTATCTTGAGGGAAAAAAGTGTAACCGGTTGGCTATACATGTCCAACATCTTTCTAGTCTCCCACGTGTTCTTCAGCCATTCTGGGATGAAACTGTTTTTACTGAGCCATCAAGGTGGAAAGGATCAGATGAGAGGGACAGCAAGTTCTTTGAGCCTGTTCAGTGGAAAAGCTACTCGCATGTATGCACAGTTGGTATCAAATATGACCCTAGCTGGGATCAAGGAGCAGGTGGTGTCTTTGTTGTGACTGGTGCACAACTTCAAGTGAGAGGTGGCTGGGCTAAAAGTGTTTTGCATCTGAGATTGCTTTTCACTAGGCTACCAAATTGTAGCATTCAGAAGTCTGTTTGGGGCCATGCACCTGCTGTTTCACAGAGATCAGGATTCCTTTCAACACTCAGCTCCACTTTTTCACTTCAACACCCCCACCAAAGCCAGTCCCAGTTGTGCTGAACTCAGGTATTTATCCTGATGGTCCACCTGTTCCTGTACGATCTCAGAAGCTTCTAAAATTTGTAGACACTGCTGAAGTTAGCAGGGGACCTCAGGACATGCCTGGGCATTGGCTAGTGACTGCAGCCAAACTGGATATTGACAGAGGCAAAATATCATTACAGGTCAAGTTTTCTCTTTTGAATTATTGCAGTGATAGATCACCTACTTCTTGTGGAAGACTATAGGGGGATTGCTTTTATTCCAAGTTCACTTCCTATCCTCATCCATAGAAGTACAAGCGAATAGCTGTAAATATTCCTATTCAAGATAGCTCTCGATTCTTTTAAATTTTCATGTATAACCTTGACAAATTCTACATGATCAGTTTTTTAGTGTAACGTAGAAGTGAGAGTATTCATAAGTATAATTCAAATGCTATATACCAGGTTTTTCATGTAAAGCTGAAGAATTATTTTTGAGACACTTTCATTGAAAACAGGAAGGCACTTA

Ace file of the UniGene sp_v3.0_unigene1640 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Membrane attack complex component/perforin/complement C9 [Medicago truncatula]	-	-	0.0	50%
FL-Next	sp=MACPF domain-containing protein At1g14780; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	49%
Sma3	MAC/Perforin domain containing protein	-	-	1.134e-08	-

Source	Gene names
Sma3	AT4g24290; At1g14780; At1g28380; At1g29690; At4g24290; F15D2.24; GSVIVT00003061001; GSVIVT00019234001; GSVIVT00030609001; GSVIVT00033508001; GSVIVT00035282001; GSVIVT00035793001; GSVIVT00035794001; GSVIVT00037483001; MA4_82I11.22; MBP_81C12.22; MtrDRAFT_A

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	immune response	GO:0006955	Biological Process	-
Sma3	cell death	GO:0008219	Biological Process	-
Sma3	plant-type hypersensitive response	GO:0009626	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	regulation of salicylic acid metabolic process	GO:0010337	Biological Process	-
Sma3	defense response by callose deposition	GO:0052542	Biological Process	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Oligopeptide transporter	IPR000109	-	0.0	-
Sma3	Membrane attack complex component/perforin/complement C9	IPR001862	-	0.0	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G14780.1	MAC/Perforin domain-containing protein chr1:5091020-5093873 FORWARD LENGTH=627	49%
RefSeq	Arabidopsis thaliana	NP_172931.1	MAC/Perforin domain-containing protein [Arabidopsis thaliana]	49%
RefSeq	Populus trichocarpa	XP_002303004.1	predicted protein [Populus trichocarpa]	59%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q8L612

Fln msg: Separated hits, possible frame ERROR between 1989 and 2055,

Fln protein: MGQTMEDSERTAVQAVEALGRGFDFTNDFRLRFCKGNSRSRLVVLEEERKQDIVIPGGPLIRNVSTDIKCDKGERTRFKSDILEFNQMSELFNQRSSLQGKIPSGNFNSLFDFSGSWLQDATETKHLAFDGFFISLYHLHLTKTPLILKEGVKSAVPSNWDPAALARFIETFGTHIIVSVAIGGQDLVFVRQNHSSSISPAELKNHLENLGDQIFSDGRSLSPFRNNKRESRQKVPEVFSNILRSTNPVHVGSFSSTSSKDGLTIFCSKRGGIIDLRNHREWLHTVSEHPDAILFSLVPITSLLTGVVGSGFLSHAINLYLRYKPAIDELQYFLEFQVPRQWAPVYNELPLGPSTKRIFCPSLQFSFMGPKLYVRTIQVCVSRRPVIGLRLYLEGKKCNRLAIHVQHLSSLPRVLQPFWDETVFTEPSRWKGSDERDSKFFEPVQWKSYSHVCTVGIKYDPSWDQGAGGVFVVTGAQLQVRGGWAKSVLHLRLLFTRLPNCSIQKSVWGHAPAVSQRSxxxxxxxxxxxxxxxxxxxxxxxLNSGIYPDGPPVPVRSQKLLKFVDTAEVSRGPQDMPGHWLVTAAKLDIDRGKISLQVKFSLLNYCSDRSPTSCGRL*

Protein Length: 619

Fln nts: TGGACTATAGAGTATGGACGGAAGATTGTGTTTGGGTTTATAGAATTCATTACAAATTCGGATTGGTTCTGAAGAATTTATTAGAAGCAATGGGTAATTGGCGGCGGAGAACGCGTGAAATTGTTGGAGTTTTCGTCGGGTTTGACTAAATCTGGTTCAGCAAAGAAGTGCGTGGGTATATTGGCGTTATAGGGTTGAAGGGATTTATGAACAATCAAGGTAGTTCGGTGCAGAGAATGCAAGAAGTAATAGATGTTTGTTTGTTTCTGAATAAATTTGTTGGTGGGTTTGTTGAGAATTGAGTTAGTGAAGGATAATGGATTTGATATGGTGTGAATTCTGATTCTGGGGTTTAAGGTGCTTGCCATTACAGATGAATTGGATGTGATGGGACACGAGACGTGTTTGCAGGTTTTCAGGCTTGATTGAGTC_-_ATGGGACAAACAATGGAAGATTCAGAGAGAACTGCAGTGCAAGCTGTGGAGGCTCTGGGGCGAGGCTTTGATTTCACAAATGATTTCAGGCTTAGGTTCTGCAAGGGCAATTCAAGGAGTCGACTGGTAGTTTTGGAGGAGGAGCGAAAGCAAGATATTGTGATTCCTGGAGGCCCTTTGATACGAAATGTGTCGACAGACATTAAGTGTGACAAGGGAGAACGTACCAGATTTAAGTCTGATATTTTGGAATTCAATCAGATGTCTGAGCTATTCAATCAGAGGTCCTCTCTACAAGGGAAGATCCCCTCTGGCAACTTCAATTCTCTGTTTGATTTCAGTGGGTCGTGGTTGCAAGATGCTACAGAAACCAAACACCTGGCCTTTGATGGCTTCTTTATTTCCCTATATCATCTACACCTTACAAAGACGCCACTCATCCTCAAGGAAGGCGTCAAATCGGCCGTTCCTTCTAATTGGGATCCTGCGGCACTTGCCAGGTTTATTGAAACTTTTGGGACACACATTATTGTGTCCGTGGCAATTGGAGGTCAGGATCTGGTGTTTGTAAGGCAGAATCATTCCTCCAGTATTTCACCTGCTGAACTTAAAAATCATCTGGAAAACCTGGGAGATCAAATATTTTCAGATGGCCGTAGCCTTTCGCCCTTCCGTAATAATAAGAGAGAAAGCAGACAGAAGGTTCCAGAGGTTTTTAGCAACATTTTACGATCTACAAATCCTGTGCATGTGGGCAGCTTTTCTTCAACGTCCAGCAAGGATGGATTGACAATCTTTTGCTCTAAAAGGGGTGGAATTATAGATTTACGCAATCACCGTGAGTGGCTTCATACTGTATCCGAGCATCCAGATGCAATACTTTTCTCACTAGTTCCAATTACATCCCTTTTGACAGGGGTTGTTGGCAGCGGTTTCTTAAGTCATGCTATCAATCTATACCTTCGTTACAAACCAGCCATAGATGAATTGCAATATTTCTTGGAATTCCAAGTTCCTCGGCAATGGGCCCCAGTTTACAATGAGTTACCACTTGGGCCTTCTACAAAGAGAATATTCTGTCCTTCATTACAGTTCAGCTTTATGGGCCCCAAACTTTATGTTCGCACAATTCAGGTTTGTGTGAGTAGAAGACCTGTAATTGGGTTACGGTTGTATCTTGAGGGAAAAAAGTGTAACCGGTTGGCTATACATGTCCAACATCTTTCTAGTCTCCCACGTGTTCTTCAGCCATTCTGGGATGAAACTGTTTTTACTGAGCCATCAAGGTGGAAAGGATCAGATGAGAGGGACAGCAAGTTCTTTGAGCCTGTTCAGTGGAAAAGCTACTCGCATGTATGCACAGTTGGTATCAAATATGACCCTAGCTGGGATCAAGGAGCAGGTGGTGTCTTTGTTGTGACTGGTGCACAACTTCAAGTGAGAGGTGGCTGGGCTAAAAGTGTTTTGCATCTGAGATTGCTTTTCACTAGGCTACCAAATTGTAGCATTCAGAAGTCTGTTTGGGGCCATGCACCTGCTGTTTCACAGAGATCAGGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGCTGAACTCAGGTATTTATCCTGATGGTCCACCTGTTCCTGTACGATCTCAGAAGCTTCTAAAATTTGTAGACACTGCTGAAGTTAGCAGGGGACCTCAGGACATGCCTGGGCATTGGCTAGTGACTGCAGCCAAACTGGATATTGACAGAGGCAAAATATCATTACAGGTCAAGTTTTCTCTTTTGAATTATTGCAGTGATAGATCACCTACTTCTTGTGGAAGACTATAG___GGGGATTGCTTTTATTCCAAGTTCACTTCCTATCCTCATCCATAGAAGTACAAGCGAATAGCTGTAAATATTCCTATTCAAGATAGCTCTCGATTCTTTTAAATTTTCATGTATAACCTTGACAAATTCTACATGATCAGTTTTTTAGTGTAACGTAGAAGTGAGAGTATTCATAAGTATAATTCAAATGCTATATACCAGGTTTTTCATGTAAAGCTGAAGAATTATTTTTGAGACACTTTCATTGAAAACAGGAAGGCACTTA

Fln Alignment:

Contig1640___AVEALGRGFDFTNDFRLRFCKGNSRS----RLVVLEEERKQDIVIPGGPLIRNVSTDIKCDKGERTRFKSDILEFNQMSELFNQRSSLQGKIPSGNFNSLFDF-SGSWLQDATETKHLAFDGFFISLYHLHL-TKTPLILKEGVKSAVPSNWDPAALARFIETFGTHIIVSVAIGGQDLVFVRQNHSSSISPAELKNHLENLGDQIFSDGRSLSPFRNNKR----ESRQKVPEVFSNILRSTNPVHVGSFSSTSSKDGLTIFCSKRGGIIDLRNHREWLHTVSEHPDAILFSLVPITSLLTGVVGSGFLSHAINLYLRYKPAIDELQYFLEFQVPRQWAPVYNELPLGPSTKRIFC-PSLQFSFMGPKLYVRTIQVCVSRRPVIGLRLYLEGKKCNRLAIHVQHL-SSLPRVLQPFWDETVFTEPSRWKGSDE--RDSKFFEPVQWKSYSHVCTVGIKYDPSWDQGAGG-------VFVVTGAQLQVRGGWAKSVLHLRLLFTRLPNCSIQKSVWGHAP-AVSQRSGxxxxxxxxxxxxxxxxxxxxxxLNSGIYPDGPPVPVRSQKLLKFVDTAEVSRGPQDMPGHWLVTAAKLDIDRGKISLQVKFSLLN
Q8L612______________AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF-NVFDDKQTVAFNNFS-INSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGxxxxxxxxxxxxxxxxxxxxxxLDSGVFPGGPPVPANN-KIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLCLHVKFALLH

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
1047	40		60		1.0
1207		88		11	0.923564
1262		11	88		0.923564
1263		11	88		0.923564
1284		62		37	1.0
1709	83		16		0.994512

SSRs (tot: 1)

Start position	End position	Sequence	Length
2285	2297	AAAC AAAC AAAC A	13

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain