SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene1477

Length: 2031 nt

UniGene Fasta

>sp_v3.0_unigene1477
GAGAATCCGGTTTTTTCAGCACTTTGCGAGCTGTTTATATCTGTCGGCCTTCCATGTATCAGTGACTCGGCCTTTATTGGATTAAAAGATTGCGTAGGGTCTCTGCGGCGTGAAGTGTTCCGCACGTTTCTACGGGCTCGGGCTTTTTGCTTAGGAGTGACGCGGGCAGCAGTTCTCTTTGGAATTACGGCTGTGTAACTGAAGCGGTATAGCTCTGAGGCGGCCTTGGGCAGGATTTTAGACGGGCCGAGAAATGGATGGTTAGTTCTGAGAAGTATGCGAGGGAAGGAGAATTATTGCAAGAAAGAGATGGACGGTTAAATAAATGCTGAGGTGTTATGTTTTGAGGCAGTAACACGAATCCAAGATCGCAAGGTTTTCTGCTTCAAGAATGAGCAAGTTTATTGAAACAGTGTATTAATTTGGTAGCTTTGTATGGAAGATGGACTTAAGGGGTTGGTTGCACAAGGTGCCAGGAGTATCCAGTTGCAGTCATTATTTCGACAGTTCACTTCCAGGTGATTGGGCAAGGAGGAAACTATATCGAAACAAAGTTGCATCAGTGGTGGTGATACTGGTGTCAGCTTTGTTCCTTGGTGGCGCTTTTGTTAGCGAAAATTATGCAGAAACAGTATCTAGGTGGGGTGGCCCATCTTCTTTAGTGACCTCCTCCCCGTTGAAGGAATGTGAGAAGGAGCCTCAAAGGCAGGAGGAGTATGAAAGGCATCTTCCTCAAAGGCAGAAGGAGTATGAAAGGCATCTTCCTTATGGTAGTGAGCCATTGCCAAGAGGCATAGTGCAGGAAACATCAGACTTGGAGATGCGACCACTGTGGGGTGTTTCAAAAAAAAGGTCACGAGTAAAGAGTCCGCAGAACTTGCTTGCCATTGCAGTTGGAATAAAACAGAAGGAGACTGTAAATCAGATTGTTCAGAAGTTTCCTTCAAAGAACTTCACAGTGATGCTGTTCCATTATGATGGGGTCACAGATAAATGGAGTGATTTGGAGTGGAGCAACAGAGCTATTCATATAGTGGCAATCAATCAAACCAAGTGGTGGTTTGCGAAGCGTTTCCTGCATCCAGACGTAGTTGCTGACTACAAATACATTTTCCTTTGGGATGAGGATCTCGGAGTTGAAAATTTCCATGCAGACCGATACTTGGCTATCATGGAGGATGAAGGATTAGAAATTTCTCAGCCAGGATTGGACTCTAATAAATCAGAAGTGCATCATCGTATCACTGTACGCAGTCCACGGTCAAGAGTTCATAGAAGGGTCTACAAACTACGAGGTAGCGGGCGGTGTTATGGAAATAGTACAGGACCTCCTTGTACTGGGTGGGTGGAAATGATGGCTCCTGTGTTCACAAGAGTTGCTTGGCGCTGTGCATGGTACATGATCCAGAATGATTTGGTTCATGCATGGGGTGTAGACATGAAGCTGGGTTACTGTGCACAGGGAGATCGAAGCAAGAAAGTTGGAGTCATAGATAGTGAATATATAGTGCATAAAGGAATACCATCACTCGGGGGATTAGATGATTCTAAGACAATGTTGGCGACACCTGATACTCATATATCATCAAAGAGTTCATTTACTTCCTCCATTACGGCTCCAGCTGAATCTGCATTTAGTGAGAGGGCAGAGGTGCGCAGGCAATCTTACAGTGAGTTGGATATTTTCAAACGGAGGTGGGCCAAGGCTGTCAGCGAAGACAAGTGCTGGGTTGATCCATACTCGCAGCCTGTACATAACACTGTCAGTAAATTCTATTCAAAGGGAGAGAAAGAATTGGGTGTGAAGAAAAATACTTCAAATAGTTGACTCAAACTTAACAATTCATTGTGAACCGGTTACTTAGAAATCACAGAGGGAAAATGCCAGGATATAAAAGAAATTCAGTTTTAAATTCACTTCTTATTAGTTGTCATCTTGTAAAATGCTATTTGTATCAATATGAAATATGTACATCATCCCAATTCTTTTTTGATAATAGAAATATAAATTCTGGCTCGTCGTTCTCTTTG

Ace file of the UniGene sp_v3.0_unigene1477

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	DUF707 domain containing protein	-	-	0.0	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	63%
Sma3	Lysine ketoglutarate reductase trans-splicing related 1	-	-	7.275e-19	-

Source	Gene names
Sma3	AT1G67850; AT3G26440; AT4g12840; AT4g18530; At1g08040; At1g11170; At1g13000; At1g61240; At1g61240/F11P17_22; At1g67850; At2g28310; At3g26440; At3g27470; At3g27473; At4g18530; B1206D04.27-1; B1206D04.27-2; F28J12.190; F3F19.3; GSVIVT00004646001; GSVIVT0000

Source	GOs	Term	Type	Identity
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	hydroxymethylglutaryl-CoA synthase activity	GO:0004421	Molecular Function	-
Sma3	protein dimerization activity	GO:0046983	Molecular Function	-
Sma3	isoprenoid biosynthetic process	GO:0008299	Biological Process	-
Sma3	protein transport	GO:0015031	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Ribosomal protein S17	IPR000266	-	-
Sma3	Peptidase M14, carboxypeptidase A	IPR000834	-	-
Sma3	Zinc finger, BED-type predicted	IPR003656	-	-
Sma3	SCAMP	IPR007273	-	-
Sma3	Protein of unknown function DUF707	IPR007877	-	-
Sma3	HAT dimerisation	IPR008906	-	-
Sma3	Nucleic acid-binding, OB-fold	IPR012340	-	-
Sma3	Hydroxymethylglutaryl-coenzyme A synthase, N-terminal	IPR013528	-	-
Sma3	Hydroxymethylglutaryl-coenzyme A synthase C-terminal	IPR013746	-	-
Sma3	Thiolase-like, subgroup	IPR016038	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G12840.1	Protein of unknown function (DUF707) chr4:7533621-7535832 REVERSE LENGTH=395	51%
RefSeq	Arabidopsis thaliana	NP_193020.6	uncharacterized protein [Arabidopsis thaliana]	51%
RefSeq	Populus trichocarpa	XP_002328723.1	predicted protein, partial [Populus trichocarpa]	59%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNZ7

Fln msg: your sequence is longer than subject: 462 - 336

Fln protein: MDLRGWLHKVPGVSSCSHYFDSSLPGDWARRKLYRNKVASVVVILVSALFLGGAFVSENYAETVSRWGGPSSLVTSSPLKECEKEPQRQEEYERHLPQRQKEYERHLPYGSEPLPRGIVQETSDLEMRPLWGVSKKRSRVKSPQNLLAIAVGIKQKETVNQIVQKFPSKNFTVMLFHYDGVTDKWSDLEWSNRAIHIVAINQTKWWFAKRFLHPDVVADYKYIFLWDEDLGVENFHADRYLAIMEDEGLEISQPGLDSNKSEVHHRITVRSPRSRVHRRVYKLRGSGRCYGNSTGPPCTGWVEMMAPVFTRVAWRCAWYMIQNDLVHAWGVDMKLGYCAQGDRSKKVGVIDSEYIVHKGIPSLGGLDDSKTMLATPDTHISSKSSFTSSITAPAESAFSERAEVRRQSYSELDIFKRRWAKAVSEDKCWVDPYSQPVHNTVSKFYSKGEKELGVKKNTSNS*

Protein Length: 463

Fln nts: GAGAATCCGGTTTTTTCAGCACTTTGCGAGCTGTTTATATCTGTCGGCCTTCCATGTATCAGTGACTCGGCCTTTATTGGATTAAAAGATTGCGTAGGGTCTCTGCGGCGTGAAGTGTTCCGCACGTTTCTACGGGCTCGGGCTTTTTGCTTAGGAGTGACGCGGGCAGCAGTTCTCTTTGGAATTACGGCTGTGTAACTGAAGCGGTATAGCTCTGAGGCGGCCTTGGGCAGGATTTTAGACGGGCCGAGAAATGGATGGTTAGTTCTGAGAAGTATGCGAGGGAAGGAGAATTATTGCAAGAAAGAGATGGACGGTTAAATAAATGCTGAGGTGTTATGTTTTGAGGCAGTAACACGAATCCAAGATCGCAAGGTTTTCTGCTTCAAGAATGAGCAAGTTTATTGAAACAGTGTATTAATTTGGTAGCTTTGTATGGAAG_-_ATGGACTTAAGGGGTTGGTTGCACAAGGTGCCAGGAGTATCCAGTTGCAGTCATTATTTCGACAGTTCACTTCCAGGTGATTGGGCAAGGAGGAAACTATATCGAAACAAAGTTGCATCAGTGGTGGTGATACTGGTGTCAGCTTTGTTCCTTGGTGGCGCTTTTGTTAGCGAAAATTATGCAGAAACAGTATCTAGGTGGGGTGGCCCATCTTCTTTAGTGACCTCCTCCCCGTTGAAGGAATGTGAGAAGGAGCCTCAAAGGCAGGAGGAGTATGAAAGGCATCTTCCTCAAAGGCAGAAGGAGTATGAAAGGCATCTTCCTTATGGTAGTGAGCCATTGCCAAGAGGCATAGTGCAGGAAACATCAGACTTGGAGATGCGACCACTGTGGGGTGTTTCAAAAAAAAGGTCACGAGTAAAGAGTCCGCAGAACTTGCTTGCCATTGCAGTTGGAATAAAACAGAAGGAGACTGTAAATCAGATTGTTCAGAAGTTTCCTTCAAAGAACTTCACAGTGATGCTGTTCCATTATGATGGGGTCACAGATAAATGGAGTGATTTGGAGTGGAGCAACAGAGCTATTCATATAGTGGCAATCAATCAAACCAAGTGGTGGTTTGCGAAGCGTTTCCTGCATCCAGACGTAGTTGCTGACTACAAATACATTTTCCTTTGGGATGAGGATCTCGGAGTTGAAAATTTCCATGCAGACCGATACTTGGCTATCATGGAGGATGAAGGATTAGAAATTTCTCAGCCAGGATTGGACTCTAATAAATCAGAAGTGCATCATCGTATCACTGTACGCAGTCCACGGTCAAGAGTTCATAGAAGGGTCTACAAACTACGAGGTAGCGGGCGGTGTTATGGAAATAGTACAGGACCTCCTTGTACTGGGTGGGTGGAAATGATGGCTCCTGTGTTCACAAGAGTTGCTTGGCGCTGTGCATGGTACATGATCCAGAATGATTTGGTTCATGCATGGGGTGTAGACATGAAGCTGGGTTACTGTGCACAGGGAGATCGAAGCAAGAAAGTTGGAGTCATAGATAGTGAATATATAGTGCATAAAGGAATACCATCACTCGGGGGATTAGATGATTCTAAGACAATGTTGGCGACACCTGATACTCATATATCATCAAAGAGTTCATTTACTTCCTCCATTACGGCTCCAGCTGAATCTGCATTTAGTGAGAGGGCAGAGGTGCGCAGGCAATCTTACAGTGAGTTGGATATTTTCAAACGGAGGTGGGCCAAGGCTGTCAGCGAAGACAAGTGCTGGGTTGATCCATACTCGCAGCCTGTACATAACACTGTCAGTAAATTCTATTCAAAGGGAGAGAAAGAATTGGGTGTGAAGAAAAATACTTCAAATAGTTGA___CTCAAACTTAACAATTCATTGTGAACCGGTTACTTAGAAATCACAGAGGGAAAATGCCAGGATATAAAAGAAATTCAGTTTTAAATTCACTTCTTATTAGTTGTCATCTTGTAAAATGCTATTTGTATCAATATGAAATATGTACATCATCCCAATTCTTTTTTGATAATAGAAATATAAATTCTGGCTCGTCGTTCTCTTTG

Fln Alignment:

Contig1477___PYGSEPLPRGIVQETSDLEMRPLWGVSKKRSRVKSPQNLLAIAVGIKQKETVNQIVQKFPSKNFTVMLFHYDGVTDKWSDLEWSNRAIHIVAINQTKWWFAKRFLHPDVVADYKYIFLWDEDLGVENFHADRYLAIMEDEGLEISQPGLDSNKSEVHHRITXXXXXXXXXXXXYKLRGSGRCYGNSTGPPCTGWVEMMAPVFTRVAWRCAWYMIQNDLVHAWGVDMKLGYCAQGDRSKKVGVIDSEYIVHKGIPSLGGL-DDSKTMLATPDTHXXXXXXXXXXXXAPAESAFSERAEVRRQSYSELDIFKRRWAKAVSEDKCWVDPY
B8LNZ7______________PRGSGALPKGLVQRSSDLEMRSLWGNPIQEGQRKTPHNLLAIPVGIKQKDNVNKMVQKFPADNFTVMLFHYYGVVDKWSEFKWTETAIHVAAINQTKWWFAKRFLHPDIIDLYNYIFIWDEDLGVNNFHADRYLAIMEEEGLEISQPALDTSHADVHYGITKRNPRTRVHRRMYKNGGGHPCLANSIAPPCTGWVEMMAPVFSRAAWRCSWGMIQNDLVHGWGMDMKLGYCAQGDRTKKVGIIDSEYILHEGIPSLGGMHDQNVSSSGVPD---------------------NTRLEVRKRSYIEMEIFKQRWAKAVDDDDCWIDPY

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
1509		10	90		0.90298
1511			90	10	0.90298
1561		25		75	1.0
1706	20		80		0.999996
1707	10		90		0.90298
1709	10			90	0.90298
1710			88	11	0.923564
1715		87		12	0.992554
1743			11	88	0.923564
1747	88			11	0.923564
1881	55		44		1.0
1890			44	55	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain