SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene1408

Length: 1758 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene1408

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	flap endonuclease-1 [Arabidopsis thaliana] gb\|AED93576.1\| flap endonuclease-1 [Arabidopsis thaliana]	-	-	0.0	57%
FL-Next	sp=Flap endonuclease 1-A; Physcomitrella patens subsp. patens (Moss).	-	-	0.0	63%

Source	ECs	Term	Type	e value	Identity
Sma3		EC:3.1.-.-	-	9.509e-06	-

Source	Gene names
Sma3	CHLREDRAFT_130834; F21E10.3; FEN-1a; FEN-1b; FEN1; Fen1; GSVIVT00022496001; LOC_Os03g61820; LOC_Os05g46270; MICPUCDRAFT_4172; MICPUN_96332; OSJNBa0052K01.23; OSJNBa0096I06.25; OSTLU_42373; Os03g0834000; Os05g0540100; OsI_14202; OsI_20807; Ot08g02230; PHAT

Source	GOs	Term	Type	Identity
Sma3	intracellular	GO:0005622	Cellular Component	-
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	magnesium ion binding	GO:0000287	Molecular Function	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	nuclease activity	GO:0004518	Molecular Function	-
Sma3	endonuclease activity	GO:0004519	Molecular Function	-
Sma3	5'-3' exonuclease activity	GO:0008409	Molecular Function	-
Sma3	manganese ion binding	GO:0030145	Molecular Function	-
Sma3	sequence-specific DNA binding	GO:0043565	Molecular Function	-
Sma3	DNA repair	GO:0006281	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	5'-3' exonuclease, N-terminal	IPR002421	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Helix-hairpin-helix DNA-binding motif, class 1	IPR003583	-	-
Sma3	DNA repair protein (XPGC)/yeast Rad	IPR006084	-	-
Sma3	XPG N-terminal	IPR006085	-	-
Sma3	XPG/RAD2 endonuclease	IPR006086	-	-
Sma3	Helix-hairpin-helix motif, class 2	IPR008918	-	-
Sma3	Tetratricopeptide-like helical	IPR011990	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT5G26680.1	5'-3' exonuclease family protein chr5:9311882-9315458 REVERSE LENGTH=453	0.0	61%
RefSeq	Arabidopsis thaliana	NP_001154740.1	flap endonuclease-1 [Arabidopsis thaliana]	0.0	61%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: sp_plants

Fln subject: A9S0B8

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 53 aas, putative chimeric sequence, your sequence is shorter than subject: 377 - 394

Fln protein: MEVAGKLLVSPLKPAKYPEKEQKISFPSISSSHRVTMGIKGLTKLLATHAPKCITEKNLESYRGQKIAIDTSICLYQFLIVIRGSDHGTLTNEYGKVTSHLQGMFYNTARLLRAGLKPAYVFDGQPPYLKSEELRERVSRSEEAVNKLEKAIEIGDDAEIGKYSKQAVKVTKQHNEDCRKLLRLMGVPVIESPSEAEAECASLCKTGKIDAVASEDLDALAFGTPRFLRHFVTQGSKKIPVMEFNLPVALEQLNLNMDQFVDLCILCGCNYCDTIRGIGPQNSMKLIRQYGSIEKISENIDKDRFQIPTYWPYEEARLLFKEPQVTPEKLQPNLEWTTPNEEGLKKFLVDENSFNYDRVMKVIENLRSTITSSSSS*

Protein Length: 378

Fln nts: ACTGCTTAAGCAGTGGTATCAACGCAGAGTACGCGGAGAACAGTTTTGTTCAATATCTAAATAGGACCCTGTTCTCTCTTCTCTTCAAACAGGCAGAAGCCTAGAATAATTTGAAGGAAAACGCTAAATAGATGAATGCAGCTAAAACATTGAAGCTTACGATCTCTTATTTGCGGA_-_ATGGAAGTGGCTGGAAAGCTGCTCGTGTCTCCGCTTAAGCCTGCAAAATATCCAGAAAAGGAGCAGAAGATTTCATTTCCGAGCATAAGTTCTTCCCACAGGGTCACCATGGGGATTAAGGGTCTGACAAAGCTCCTGGCCACCCATGCACCGAAGTGCATTACGGAAAAGAATCTGGAGAGCTATCGTGGACAGAAGATTGCCATTGATACCAGCATATGCCTCTATCAATTCCTGATTGTTATTCGTGGGAGTGACCATGGAACACTCACAAATGAATATGGTAAGGTGACAAGTCATTTGCAGGGCATGTTTTATAATACAGCAAGACTGTTACGAGCTGGTCTGAAGCCTGCTTATGTCTTTGATGGACAGCCTCCGTATTTGAAAAGCGAAGAACTTAGAGAAAGGGTTTCTAGGAGTGAGGAAGCGGTAAATAAGTTAGAAAAGGCGATTGAGATAGGTGATGATGCAGAGATTGGAAAATACAGCAAACAGGCAGTTAAGGTGACAAAACAACATAATGAGGATTGTAGAAAGCTTCTAAGACTCATGGGAGTGCCTGTAATTGAGTCTCCTAGTGAAGCAGAGGCAGAGTGTGCATCTTTGTGCAAAACTGGAAAAATTGATGCTGTAGCTTCTGAAGATCTGGATGCACTAGCCTTTGGAACTCCAAGATTTCTTCGGCATTTCGTGACACAAGGCTCAAAAAAAATTCCTGTAATGGAGTTTAATTTGCCAGTGGCATTGGAGCAGCTGAATCTTAACATGGACCAGTTTGTGGACTTGTGCATCCTTTGTGGGTGTAACTATTGTGATACCATTAGAGGTATTGGACCACAAAACTCGATGAAACTTATTCGTCAATATGGTTCAATAGAGAAGATCTCGGAGAACATAGATAAAGATAGGTTTCAAATTCCAACGTATTGGCCATATGAAGAAGCTAGACTTCTTTTCAAAGAGCCGCAAGTCACTCCTGAAAAATTGCAACCAAATCTGGAGTGGACTACTCCAAATGAAGAGGGATTAAAGAAGTTTCTTGTGGATGAAAATAGCTTTAACTATGACCGAGTTATGAAGGTTATAGAGAATCTCCGATCAACAATAACCAGCTCATCTTCATCTTGA___GAATGCATGTATCTATATGCCTCCTTGATTTCTGATGTTGATTTTTCTATAGGTTTGAACCCTTTATGAAGCCCAGGGTGGACAGATCCTAATTAAGGAAGCAAATGAGTTATTGCCGTTTCCTTCGTGAACGTGTTGCAGCCTTTTGTAGTCCAAGATAACGTGTCCTAACTGTTTCTATTCAGACTAGTATTTAGACCTTGCATGCGGATTTTATGGTGTCACGACAAAATTTAAGTCATCCTTAAATTGAATGGAATGAGCAAAGAGATGATGATGTGATATAAATGCATCCAGTTTGATGTATCATCATGTACTAGTATTAACAATCATGAATCCACTTTTTCAATGTATCATTTGAGCTGGGAATTGCATCGCACAAATGATATGGAAACTTTGTGGATATTTGTTCTTAGTTTTCTTATGAAGGATGTTCTTCTCTGTTTTGTC

Fln Alignment:

Contig1408___MGIKGLTKLLATHAPKCITEKNLESYRGQKIAIDTSICLYQFLIVIRGSDHGTLTNEYGKVTSHLQGMFYNTARLLRAGLKPAYVFDGQPPYLKXXXXXXXXXXXXXAVNKLEKAIEIGDDAEIGKYSKQAVKVTKQHNEDCRKLLRLMGVPVIXXXXXXXXXXXXLCKTGKIDAVASEDLDALAFGTPRFLRHFVTQGSKKIPVMEFNLPVALEQLNLNMDQFVDLCILCGCNYCDTIRGIGPQNSMKLIRQYGSIEKISENIDKDRFQIPTYWPYEEARLLFKEPQVTPEKLQPNLEWTTPNEEGLKKFLVDENSFNYDRVMKVIENLR
A9S0B8______________MGIKGLTKLIADNAHGAVKEQKFENYFGRKIAIDASMSIYQFLIVVGRSGSELLTNDAGEVTSHLQGMFNRTIRVLEAGLKPVYVFDGQPPDLKKRELAKRFARREDAAEDLVTAKETGNEADVEKYSKKTVKVTKQHNEDCRKLLRLMGVPVVEAPSEAEAECASLCKAEKVFAVASEDMDSLTYGSTRFLRHLMEPTSRKLPVLEFDIAKVLEGLGLNMDQFVDLCILCGCDYCDTIRGIGPQTALKMIRQHGSLEIVLENLNKDRYQVPDPWPYQEARRLFKEPLVTPPEKVPEFKWTAPDTEGLRQLLVEENGFNNDRVMKAIEKLK

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
1204			80	20	0.995493
1286	16	83			0.994512
1289	50	50			1.0
1332		16		83	0.994512
1385			66	33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain