SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene985

Length: 1838 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene985 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Dead box ATP-dependent RNA helicase, putative n=1 Tax=Ricinus communis RepID=B9S466_RICCO	-	-	0.0	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	33%
Sma3	DEAD-box ATP-dependent RNA helicase 18	-	-	1.229e-08	-

Source	ECs	Term	Type	e value	Identity
Sma3	Hydrolases, Acting on acid anhydrides, In phosphorous-containing anhydrides.	EC:3.6.1.-	-	6.183e-09	-

Source	KEGGs	Term	e value	Identity
Sma3	Purine metabolism	00230	6.183e-09	%
Sma3	Lipopolysaccharide biosynthesis	00540	6.183e-09	%
Sma3	Folate biosynthesis	00790	6.183e-09	%
Sma3	Metabolic pathways	01100	6.183e-09	%

Source	Gene names
Sma3	At1g71280; At1g71370; At5g05450; B1189A09.47; CHLREDRAFT_118990; F26A9.25; F3I17.7; GSVIVT00029362001; K18I23.26; LOC_Os01g07080; MICPUCDRAFT_18446; MICPUN_67382; OSTLU_1733; Os01g0164500; OsI_00527; Ot14g02150; P0701D05.2; PHATRDRAFT_9989; PHYPADRAFT_122

Source	GOs	Term	Type	Identity
Sma3	nucleic acid binding	GO:0003676	Molecular Function	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	ATP-dependent helicase activity	GO:0008026	Molecular Function	-
Sma3	poly(U) RNA binding	GO:0008266	Molecular Function	-

Source	InterPros	Term	Type	Identity
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	IPR000634	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3	RNA helicase, DEAD-box type, Q motif	IPR014014	-	-
Sma3		IPR014021	-	-
Sma3	HTH domain AraC-type, conserved site	IPR018062	-	-
Sma3	Ribosomal protein L34e, conserved site	IPR018065	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G05450.1	P-loop containing nucleoside triphosphate hydrolases superfamily protein chr5:1612077-1615195 FORWARD LENGTH=593	63%
RefSeq	Arabidopsis thaliana	NP_196164.1	DEAD-box ATP-dependent RNA helicase 18 [Arabidopsis thaliana]	63%
RefSeq	Populus trichocarpa	XP_002312182.1	predicted protein [Populus trichocarpa]	67%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PQ26

Fln msg: Unexpected stop codon in the beginning of your sequence, STOP codon was not found. Distance to subject end: 142 aas,

Fln protein: *NLECF*ENFSLVVALECQSGLTDKVFSVLQPSLSEATLKVLEDGGFHYCTPVQAATIPLLCSFKDVAVDAATGSGKTLAFVVPLVEILRRLTDPLNSHQVAGIIISPTRELASQIYRVIEPFVSTLPNLRCMLVVGGTDVAADLKKLKENGVNIIVGTPGRLDDIMERINILDFRRFEVLILDEADRLLDMGFQKQLSSIIGRLPKQRRTGLFSATQTEAVEELSRAGLRNPVRVEVRVEAKSKIRSSTMTDMVCSRTPEGLHIEYLKCEADEKPTQLVHFLSEHKSNKIIVYYMTCASVDYWGVVLPQLEALKGCPLIALHGRMKQSMREKALQSFTKLSAGFLLCTDVAARGLDIPDVDWIVQYDPPQDPNVFVHRVGRTARMGRSGNALAFLLPKEDAYVEFLRIRKIPLQEKKSSVTTSDIISQIRAAAKEDRDVMEKGLKAFVSFIRAYKEHHCSYIFRWKELEAGKLAMGYGLLQLPSMPELKRRTLSTDGFVPVEGIDLGAIKCKVKAREKQRQRNSAKKEQTRTHCAEKVERHKVVSNDGAGKKCSRQRRAVQAIEDEEEMERDYHLLKKLKKGVISEREFARATYTEDCLDEDDDGDEHADD

Protein Length: 613

Fln nts: CTAAAACCTCGAGTGTTTTTGAGAAAACTTCAGCCTTGTTGTGGCGTTGGAGTGTCAGTCAGGTTTAACAGACAAAGTCTTTTCCGTGTTGCAGCCCTCTCTATCAGAGGCCACTCTGAAGGTGTTGGAGGACGGCGGCTTCCATTACTGTACGCCTGTACAGGCCGCCACCATCCCTCTTTTATGCTCTTTCAAGGACGTCGCGGTCGATGCCGCCACCGGCTCCGGGAAGACTCTCGCCTTCGTCGTTCCGCTCGTGGAAATCCTCAGGCGCCTCACAGACCCTCTCAATTCTCACCAGGTTGCAGGGATTATAATTTCTCCAACAAGAGAATTGGCATCCCAAATATACCGTGTGATAGAGCCATTTGTTTCTACTCTTCCCAATCTCAGGTGCATGCTTGTAGTTGGTGGGACAGATGTGGCAGCAGATTTAAAGAAGCTAAAAGAGAACGGGGTGAACATAATTGTAGGTACACCTGGGCGCTTAGATGATATCATGGAGCGAATAAACATTCTAGACTTTCGGCGCTTTGAGGTTCTTATTTTAGATGAGGCAGATAGGCTATTGGATATGGGTTTTCAGAAACAGTTGTCATCCATTATAGGCCGTTTACCAAAGCAACGGCGGACAGGACTTTTTTCTGCTACCCAGACCGAGGCAGTTGAAGAGCTTTCAAGAGCTGGGCTTAGGAACCCAGTGAGGGTTGAAGTCAGAGTAGAAGCAAAATCTAAGATTCGATCATCCACTATGACTGATATGGTTTGCTCGAGGACACCTGAAGGGCTTCACATTGAGTATTTAAAATGTGAGGCAGATGAAAAGCCTACACAACTTGTGCATTTCTTGTCTGAACACAAGTCAAACAAAATTATTGTATACTACATGACATGTGCCTCTGTTGATTATTGGGGAGTTGTACTACCACAACTTGAAGCTCTTAAAGGATGTCCTCTGATTGCTCTTCATGGGAGGATGAAGCAGTCTATGAGGGAGAAAGCATTACAGTCTTTCACAAAGCTTTCTGCTGGATTTCTTCTATGCACTGATGTGGCGGCACGGGGTCTTGATATTCCTGATGTTGACTGGATTGTGCAGTATGATCCTCCACAAGACCCTAATGTATTTGTACATAGGGTTGGGCGAACAGCACGAATGGGAAGATCTGGGAATGCACTTGCGTTCCTCCTACCTAAGGAAGATGCTTATGTGGAGTTTTTGCGTATCCGGAAGATCCCTCTTCAAGAGAAAAAATCTTCAGTGACTACTTCTGACATTATTTCACAGATACGTGCAGCTGCAAAGGAGGATCGAGATGTAATGGAGAAGGGATTGAAGGCTTTTGTGTCATTTATTCGTGCATACAAAGAGCACCATTGTTCCTATATCTTCAGATGGAAAGAATTGGAGGCAGGGAAGCTAGCAATGGGATATGGTCTACTGCAGCTTCCATCTATGCCTGAGCTTAAGCGGCGCACTTTGTCAACTGATGGTTTTGTGCCTGTTGAAGGCATTGATCTGGGAGCGATCAAATGTAAAGTTAAAGCTCGTGAGAAACAACGTCAAAGGAACTCAGCTAAGAAAGAACAAACAAGAACTCATTGTGCAGAGAAAGTAGAACGACATAAAGTTGTGAGCAATGATGGTGCAGGGAAGAAATGCAGTAGACAGCGACGTGCTGTCCAAGCCATTGAAGACGAGGAGGAAATGGAACGGGATTATCACTTGCTTAAGAAGTTAAAGAAAGGAGTTATTAGTGAAAGAGAATTTGCAAGAGCAACATATACTGAAGATTGTTTAGATGAGGATGATGATGGGGATGAGCATGCAGATGATG

Fln Alignment:

Contig985___SVLQPSLSEATLKVLEDGGFHYCTPVQAATIPLLCSFKDVAVDAATGSGKTLAFVVPLVEILRRLTDPLNSHQVAGIIISPTRELASQIYRVIEPFVSTLPNLRCMLVVGGTDVAADLKKLKENGVNIIVGTPGRLDDIMERINILDFRRFEVLILDEADRLLDMGFQKQLSSIIGRLPKQRRTGLFSATQTEAVEELSRAGLRNPVRVEV-RVEAKSKIRSSTMTDMVCSRTPEGLHIEYLKCEADEKPTQLVHFLSE-HKSNKIIVYYMTCASVDYWGVVLPQLEALKGCPLIALHGRMKQSMREKALQSFTKLSAGFLLCTDVAARGLDIPDVDWIVQYDPPQDPNVFVHRVGRTARMGRSGNALAF
C0PQ26_____________SFREASFTDHVLRELERAGFSEPTAIQAQGWPMALKGRDLIGIAETGSGKTLAYLLPAIVHINAQPHLAHGDGPIVLVLAPTRELAVQIQQESVKF-GTSSKIKSTCIYGGAPKGPQIRDL-QRGVEIVIATPGRLIDMLEARHT-NLRRVTYLVLDEADRMLDMGFEPQIRKIITQIRPDRQTLYWSATWPREVENLARQFLHNPYKVIIGSADLKANHAISQIVEVVS---------EY------EKYPRLIKLLEEIMDGSRILIFMETKKGCDQ---VTKQLR-MDGWPALSIHGDKSQAERDWVLGEFKAGKSPIMTATDVAARGLDVKDIKCVINYDFPGSLEDYVHRIGRTGRAGAKGTAYSF

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
536		80		20	0.995493
755	20		80		0.995493
1009	20		80		0.995493
1010		20		80	0.995493
1011	20		80		0.995493
1013	80			20	0.995493
1185	33		66		0.997452
1270	33			33	0.997753
1603			80	20	0.995493
1784			40	60	1.0
1785	40			60	1.0
1786		60	40		1.0
1788			40	60	1.0
1789	40	60			1.0
1790		40		60	1.0
1791	40			60	1.0
1792		60	40		1.0
1796	40		60		1.0
1797	40	60			1.0
1798		60	40		1.0
1800		60		40	1.0
1803		40		60	1.0
1804	60		40		1.0
1805	40		60		1.0
1807	60		40		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain