SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene869

Length: 2398 nt

UniGene Fasta

>sp_v3.0_unigene869
TACTGTTTAAATTTGATAAATCAGTCAACGATACCAGAGTTTCGTTGAAATTAGCAATTCACCGTGGTTTATTAAAAAAAAGGTCAGCCGATAGTCCAGGTTTGTTTTTTTAAATATTTCAAGAGCATCCAACACCATGTCGCTGAGCCCGTCCATCCTTCTCTAGCGAACCGTTCGTCTTGTTTTGCAAGGTTATTATTATGACTATTACCGCTGCCATGTATTCCGTTTCGATTATTCATGATTGTGGTTATTATCGAAGGGGACCTCGAAATCCAAGCGCTCCACATGTAAACGAGCGACGGTTGCATCATTCTTGTGGCCGGTTTTTGCAATTTTTGAACCCTTTTGAAGGTTTGAGCCGATACTGGGCGGATCCTAGGTGGATCCGAATTCGCACACGATCTGGGGATCTGCGGTTCAATATCCCAGGGTGGACGGATCAGTTTGTGGACCAACTCAAGCTCAAGGAAATGGAGTCTGAGAGGTATGAGAAAGAGTTAGCAGTTGATTCCAACAAGGGCATTTCCTCTTTTGAGGACATCTCTCCAGTATTGGCAAAGGAGCTTCTGCAAGATGCACTCGTGTGGGCTTCCTTACATGGCCTTGTGGTTGGCGATAGGGCTGTACAGGGTTCTGGACAAATCCCGGGTGTTGGGTTGGTGCATGCTCCGTTTGCTCTCTTGCCAACACCTTTTCCAGAAGGCTGTTGGAAACAGGCCTGTGATTTGGCACCCTTATTTAATGAGCTTGTAGATCGAGTCAGCATGGACGGAGTTTTTCTGCAAGAGTCACTTTCCAGGACAAAGACAGCTGATGCTTTCACTGCTCGACTCTTAGATATCCATTCTAGTATAATGAAAGATAACATAAAACAGGAAATACGCTTGGGTCTTCATCGTTCAGATTATATGCTTGATATAGCTACAGGAAAACTGCTACAAGTAGAGCTTAATACAATATCATCGTCTTTTGCAGGACTTGGTTGCCTTGTTAGTCAGCTTCACAGATACTTGATCAATCATTCTGGGAAGAAACTTAGCTTGGATTCAAAAAGAGTACCTGAAAACACAGCAGCAAACAAATTTGCAGAGGCATTGGCCCTAGCCTGGAAGGAGTTCAATGACTTAAGTTCTGTAGTACTGGTAGTTGTGCAGCCAGAGGAGCGTAATATGTATGATCAACACTGGATTTGCATGGTGCTGAAAGAATCATATGCGGTGCCATCTATCCGGAAAACTCTAGCTGAAATTGATGCTGAGGGTGAACTTTTATCAAATGGAACTCTTCACATAGGACAACGAGCTGTTGCTGTTGTTTACTTCAGGGCTGGATATACACCTGTTGATTATCCCTCTGAAGCAGAGTGGAGAGCTAGACTAATGTTGGAGCAGTCGTCTGCCATCAAATGCCCTTCAATTTCATATCATTTAGCAGGCACAAAAAAAATTCAACAAGAATTGGCTAAACCTCATGTTCTGGAAAGGTTCATCGATAATAAAGATGACGTTTCTAATCTTAGGAAATGCTTTGCTGGATTATGGAGCTTGGACAATAATGCAAATGTTATAGAAGATGCCATCAGAAGGCCAGAATCATTTGTGCTGAAGCCCCAGCGTGAAGGAGGGGGAAATAACATATATGGAAATGATGTCAAGGAAAAATTACTTGAGTTGAAAAGAAATGGTGGTGATGGACATGCTGCTTACATACTAATGCAAAGGATCTTTCCTCCAATTCATGTGTCATATCTTGCCCGTGGTGGTATCTATCAACACGGTGAAACTGTGTCTGAACTTGGCATATTTGGTGCATATTTACGGAGTAAAGACAATGTGATACTAAACAACCAAAGTGGTTATCTCATGAGAACAAAAGTTTCTTCCTCCAATGAGGGTGGTGTTGCTGCAGGCTTTGCTGTTGTGGATAGTCTCTATCTAATTTGAGTTATCTTCAATTGTACCCTGTGAATTTATGGCATGGAAATTTAAGGAACCTTTTCATTGTCTAATTGGTATTGATTTTTCCATTTCAGTTCTCTTGGAAATGAGAATGTCAACTCCGTTACTTTGCTCGTAGCATGTTCACCTATTGGTTGTCCCACCTCCTGAGAGAATATTCAAATGAAAATAGAATGTATGCTGGCCTAGTTAGAAACTGGTAAAGCTTAGCAAAATTTAGAAGTGGTTCTGTAGCTTGAAGGGTGAAATGTATTGCGTCCTCTTTGAGTAATTGGCCTTTTTAACATGAACATGAATTTATTACATATATTCATGCATAAACTATTCCTTTTCAAAAGTTATACATCAGACTTCTTCCTTGTACAAGAAGTCTCAATGCTTTGAATCTGTAGAGGCATACAACTATAATCTAGGAAATATGCAGCTCTTCTATCCCA

Ace file of the UniGene sp_v3.0_unigene869

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Glutathione synthetase, chloroplastic n=2 Tax=Brassica RepID=GSHB_BRAJU	-	-	0.0	58%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	92%
Sma3	Glutathione synthetase	-	-	1.442e-34	-

Source	ECs	Term	Type	e value	Identity
Sma3	Glutathione synthase.	EC:6.3.2.3	-	4.808e-35	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Glutathione metabolism	00480		4.808e-35	%
Sma3	Metabolic pathways	01100		4.808e-35	%

Source	Gene names
Sma3	At5g27380; CHLREDRAFT_189020; F21A20_90; GS; GSH1; GSH2; GSHII; GSHS1; GSVIVT00022884001; LOC_Os11g42350; LOC_Os12g16200; LOC_Os12g34380; MICPUCDRAFT_18089; MICPUN_108131; MtrDRAFT_AC150244g30v2; MtrDRAFT_AC172101g15v1; MtrDRAFT_AC172101g16v1; OSTLU_32080

Source	GOs	Term	Type	Identity
Sma3	cytosol	GO:0005829	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	glutathione synthase activity	GO:0004363	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	homoglutathione synthase activity	GO:0047983	Molecular Function	-
Sma3	glutathione biosynthetic process	GO:0006750	Biological Process	-
Sma3	response to jasmonic acid stimulus	GO:0009753	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Peptidase M14, carboxypeptidase A	IPR000834	-	-
Sma3	F-box domain, cyclin-like	IPR001810	-	-
Sma3	Glutathione synthase, substrate-binding, eukaryotic	IPR004887	-	-
Sma3	Glutathione synthase, eukaryotic	IPR005615	-	-
Sma3	ATP-grasp fold, subdomain 2	IPR013816	-	-
Sma3	Glutathione synthase, alpha-helical, eukaryotic	IPR014042	-	-
Sma3	Glutathione synthase, C-terminal, eukaryotic	IPR014709	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT5G27380.1	GSH2, GSHB glutathione synthetase 2 chr5:9668211-9670912 REVERSE LENGTH=539	0.0	60%
RefSeq	Arabidopsis thaliana	NP_568495.2	glutathione synthetase [Arabidopsis thaliana]	0.0	60%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8U7

Fln msg: your sequence is longer than subject: 582 - 187

Fln protein: MTITAAMYSVSIIHDCGYYRRGPRNPSAPHVNERRLHHSCGRFLQFLNPFEGLSRYWADPRWIRIRTRSGDLRFNIPGWTDQFVDQLKLKEMESERYEKELAVDSNKGISSFEDISPVLAKELLQDALVWASLHGLVVGDRAVQGSGQIPGVGLVHAPFALLPTPFPEGCWKQACDLAPLFNELVDRVSMDGVFLQESLSRTKTADAFTARLLDIHSSIMKDNIKQEIRLGLHRSDYMLDIATGKLLQVELNTISSSFAGLGCLVSQLHRYLINHSGKKLSLDSKRVPENTAANKFAEALALAWKEFNDLSSVVLVVVQPEERNMYDQHWICMVLKESYAVPSIRKTLAEIDAEGELLSNGTLHIGQRAVAVVYFRAGYTPVDYPSEAEWRARLMLEQSSAIKCPSISYHLAGTKKIQQELAKPHVLERFIDNKDDVSNLRKCFAGLWSLDNNANVIEDAIRRPESFVLKPQREGGGNNIYGNDVKEKLLELKRNGGDGHAAYILMQRIFPPIHVSYLARGGIYQHGETVSELGIFGAYLRSKDNVILNNQSGYLMRTKVSSSNEGGVAAGFAVVDSLYLI*

Protein Length: 583

Fln nts: TACTGTTTAAATTTGATAAATCAGTCAACGATACCAGAGTTTCGTTGAAATTAGCAATTCACCGTGGTTTATTAAAAAAAAGGTCAGCCGATAGTCCAGGTTTGTTTTTTTAAATATTTCAAGAGCATCCAACACCATGTCGCTGAGCCCGTCCATCCTTCTCTAGCGAACCGTTCGTCTTGTTTTGCAAGGTTATTATT_-_ATGACTATTACCGCTGCCATGTATTCCGTTTCGATTATTCATGATTGTGGTTATTATCGAAGGGGACCTCGAAATCCAAGCGCTCCACATGTAAACGAGCGACGGTTGCATCATTCTTGTGGCCGGTTTTTGCAATTTTTGAACCCTTTTGAAGGTTTGAGCCGATACTGGGCGGATCCTAGGTGGATCCGAATTCGCACACGATCTGGGGATCTGCGGTTCAATATCCCAGGGTGGACGGATCAGTTTGTGGACCAACTCAAGCTCAAGGAAATGGAGTCTGAGAGGTATGAGAAAGAGTTAGCAGTTGATTCCAACAAGGGCATTTCCTCTTTTGAGGACATCTCTCCAGTATTGGCAAAGGAGCTTCTGCAAGATGCACTCGTGTGGGCTTCCTTACATGGCCTTGTGGTTGGCGATAGGGCTGTACAGGGTTCTGGACAAATCCCGGGTGTTGGGTTGGTGCATGCTCCGTTTGCTCTCTTGCCAACACCTTTTCCAGAAGGCTGTTGGAAACAGGCCTGTGATTTGGCACCCTTATTTAATGAGCTTGTAGATCGAGTCAGCATGGACGGAGTTTTTCTGCAAGAGTCACTTTCCAGGACAAAGACAGCTGATGCTTTCACTGCTCGACTCTTAGATATCCATTCTAGTATAATGAAAGATAACATAAAACAGGAAATACGCTTGGGTCTTCATCGTTCAGATTATATGCTTGATATAGCTACAGGAAAACTGCTACAAGTAGAGCTTAATACAATATCATCGTCTTTTGCAGGACTTGGTTGCCTTGTTAGTCAGCTTCACAGATACTTGATCAATCATTCTGGGAAGAAACTTAGCTTGGATTCAAAAAGAGTACCTGAAAACACAGCAGCAAACAAATTTGCAGAGGCATTGGCCCTAGCCTGGAAGGAGTTCAATGACTTAAGTTCTGTAGTACTGGTAGTTGTGCAGCCAGAGGAGCGTAATATGTATGATCAACACTGGATTTGCATGGTGCTGAAAGAATCATATGCGGTGCCATCTATCCGGAAAACTCTAGCTGAAATTGATGCTGAGGGTGAACTTTTATCAAATGGAACTCTTCACATAGGACAACGAGCTGTTGCTGTTGTTTACTTCAGGGCTGGATATACACCTGTTGATTATCCCTCTGAAGCAGAGTGGAGAGCTAGACTAATGTTGGAGCAGTCGTCTGCCATCAAATGCCCTTCAATTTCATATCATTTAGCAGGCACAAAAAAAATTCAACAAGAATTGGCTAAACCTCATGTTCTGGAAAGGTTCATCGATAATAAAGATGACGTTTCTAATCTTAGGAAATGCTTTGCTGGATTATGGAGCTTGGACAATAATGCAAATGTTATAGAAGATGCCATCAGAAGGCCAGAATCATTTGTGCTGAAGCCCCAGCGTGAAGGAGGGGGAAATAACATATATGGAAATGATGTCAAGGAAAAATTACTTGAGTTGAAAAGAAATGGTGGTGATGGACATGCTGCTTACATACTAATGCAAAGGATCTTTCCTCCAATTCATGTGTCATATCTTGCCCGTGGTGGTATCTATCAACACGGTGAAACTGTGTCTGAACTTGGCATATTTGGTGCATATTTACGGAGTAAAGACAATGTGATACTAAACAACCAAAGTGGTTATCTCATGAGAACAAAAGTTTCTTCCTCCAATGAGGGTGGTGTTGCTGCAGGCTTTGCTGTTGTGGATAGTCTCTATCTAATTTGA___GTTATCTTCAATTGTACCCTGTGAATTTATGGCATGGAAATTTAAGGAACCTTTTCATTGTCTAATTGGTATTGATTTTTCCATTTCAGTTCTCTTGGAAATGAGAATGTCAACTCCGTTACTTTGCTCGTAGCATGTTCACCTATTGGTTGTCCCACCTCCTGAGAGAATATTCAAATGAAAATAGAATGTATGCTGGCCTAGTTAGAAACTGGTAAAGCTTAGCAAAATTTAGAAGTGGTTCTGTAGCTTGAAGGGTGAAATGTATTGCGTCCTCTTTGAGTAATTGGCCTTTTTAACATGAACATGAATTTATTACATATATTCATGCATAAACTATTCCTTTTCAAAAGTTATACATCAGACTTCTTCCTTGTACAAGAAGTCTCAATGCTTTGAATCTGTAGAGGCATACAACTATAATCTAGGAAATATGCAGCTCTTCTATCCCA

Fln Alignment:

Contig869___MLEQSSAIKCPSISYHLAGTKKIQQELAKPHVLERFIDNKDDVSNLRKCFAGLWSLDNNANVIEDAIRRPESFVLKPQREGGGNNIYGNDVKEKLLELKRNGGDGHAAYILMQRIFPPIHVSYLARGGIYQHGETVSELGIFGAYLRSKDNVILNNQSGYLMRTKVSSSNEGGVAAGFAVVDSLYL
D5A8U7_____________MLEQSSATKCPSISYHLAGTKKIQQELAKPHVLERFIDNKDDIANLRKCFAGLWSLDNNANVIEDAIRRPEAFVLKPQREGGGNNIYGNDAKEKLLEMKRNGGDGHAAYILMQRIFPPFHVSYLVRGGIYHHGETFSELGIFGAYLRNKDNVLLNNQSGYLMRTKISSSDEGGVAAGFAVVDSLYL

SNPs (tot: 9)

Position	A %	C %	G %	T %	Probability
386			66	33	0.997452
821		30		70	1.0
1367	15		84		0.999986
1552	83		16		0.994512
1683	88		11		0.923564
1696		10	90		0.90298
2139	55		44		1.0
2154			52	47	1.0
2306	50	50			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain