SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene458

Length: 3188 nt

UniGene Fasta

>sp_v3.0_unigene458
TATTTTGAGCTACCCTATTTTAGGGTTTGAGGTTTAGAAGAGTTTGTGGAAACTAGGGTTTTGGTGTAGAGTTGTTTTTGTTAAAGCGCAATTCAATAGGAGATACATCGCGAAAAACCTTCAAGTTGTTTCTTCTTCTGAAACCTAGTCGTTCCATCGAGCTGAAGCGGGAGAAAGGAGTATGCCCGCTTTAGAAATCACCCACAACTCATCTCCTTCTTCTGATACGAAGAAGAAGATGAAGCACGTCGGCTCGTCTGGCGAGAAGAAGCACAAGAAGAAGAAGGATAGGCAGGGCGAGGAAGAGGAGGTGGACCATGCCATGAACGGAAGTGAATCTATGACCTCCAAGAGGAAATTGTCCAAGGAGGCCGTAGGTGATGATTCCAGTCCCAAGCGCAGCAAGAAGAAGAAGAAGACAGGGGGCAGCGATGTCGAAGAGGAAGAAGAAGAAGAGAAGGAAGAGAAGGAAGAGAATCCCAACGCGGTTTCTAATTTCAATATTTCGGAGCCACTGAGGCAGAAGCTCAAGTCCAAGGGGATTGAGTCACTCTTCCCCATCCAGGCAATGACCTTCGAAATTATCTTCAATGGCTCGGACATGGTCGGTCGAGCTCGTACAGGACAGGGTAAAACATTGGCATTTGTGTTGCCTATCGTGGAGTCATTAACAAATGGTCCGAATGCCAAAGGTAGGAATACAGCCTATGGAAGGGCCCCCAGTGTGATTGTGTTAGTTCCAACAAGGGAGCTTGCAAAACAGGTCCATGCTGACTTTGAGGTTTATGGTGGAGCTGTTGGCCTTCATTCATCCTCTGTATATGGAGGTGCTCCCTATGGTCCACAGGAATATGCATTGCGTAGAGGTGTGGATATCATTGTCGGGACACCTGGGCGCATTAAGGATCATATTGAAAGAGGGACATTGAATCTGAAATCCTTGAAATTTCGTGTCTTGGATGAGGCTGATGAAATGCTGAACATGGGCTTTGTAGAGCATGTTGAGACCATTCTTGGAAAAGTTGAGGATGTAAGCAAGGTTCAGACACTTCTTTTTAGTGCAACAATGCCAGATTGGGTAAAGAAGATTGCATCAAGGTTCTTGAAACCTGACAGAAAAACTGCAGATCTTGTGGGAGATGATAAGATGAAAGCTAGTAGAAGTGTGAGACACATCGTTCTTCCTTGTTTTTATTCAGCAAGAACACAAGTTATACCAGATGTTATTCGCTGCTATAGCAGTGGGGGAAGTGTGATTGTTTTTACTGAGACAAAGAATGATGCATCTGAACTCGCTGGATCTTTGCAAAATGCTCGTGCTTTGCATGGGGATATACAACAATCACAACGTGAAGTGATTCTTGCTGGATTCCGTTCGGGAAAGTTTCCACTCCTAGTTGCAACAGATGTTGCGGCACGCGGTTTGGACATCAATGATGTTCAGCTTATAATTCAGTGTGAACCACCAAGAGATGTGGAAACATACATTCATCGATCTGGGCGGACTGGTAGAGCAGGCAACACTGGAGTCTCTGTGATGCTTTATGATAGAAAGAAGCAATATATGATTTCTCAGATAGAAAGGCAGGCTGGTGTAAAATTTGAGCGTGTATCTGCTCCTCAGCCAGGAGAGGTTGCTAAAGCTACTGGATCAACTGCTGCAGAAAGTATTACTAGTGTCAGTGACAGTGTGCTTCCCATTTTTCGGCAATCAGCTGAGGATCTGGTGAAGAAATCTGATCTTTCACCTGTGGATATCATTGCAAAAGCACTAGCAAAGATTGCAGGATACACAGAATTCAAGAGAAGATCATTACTGTCCTCTCAAGAGGGTTTTGCAACCTTAATTCTTAAAACTGGAAGGACTATGCATACCCCATCATATGCTTTTACCATACTAAGAAGAGTTATACCAGAGGAAACTATTGCTGGGATCAAGGGCATGTCTCTCACTGCAGATGGAACTGGTGCCGTATTTGATGTGGCAAATGAGCATGTGGATTTATTTCTTGCAGCTCAGGAAAATGCAGATGGGGTTGATATCGAATTAATTGAAAGTCTACCAGAGATGCAACAAGTTCGTAATGACCAATCAAGAGGACATGGAGGGGGTGGCTACGGTAATGGGTATGGCAGAGGAGGGCGGGGAGGATATTCCAGTGGTTCAAGAGGAGGGGGTGGTGGTTCAAGAGGAGGCCGTGGTGGGAGTTCAAGAGGAGGTCGTGGTGGTGGTTCAAGAGGAGGTCGAGGTTGGGGACGTCAGTAAGGGAATTGGTGATCATCATTTCAAAAATTTCAAATAAAGTAATGTGTATTTTAACTGGCAGAGATTCTTAACTATAGCCTGGGCTGTCCGTCATATCCTTCTTGGGCAAAAAAGAGCTACAATTAGAGAAGCCTTGGTTTATTTGTTTAGGGGTACTGTAGTGTGAAAACATTCATTAGGGCAAGGTGTATGAGTATTCAAGTCACTTGCTTATATCTTGATCTGATTATCTCTAAGACCATCATCATCCATTAGATGATGCTTTATTTTTGTACTGTTTTTCTATATGAGAAAAGAAGATGGAGCCTTCGAATATGCTTCTACACGTTTCTGCTCGAAAGCTCCCAAGCTCTGTGCTTGTTTTTGGGTCATCTTTTTTCGACCCATATAGAATTATTTTACTCCACAAGGAGTTATTTAAGGTCCTTTAGACAGGCATGAGATTTCAAGGAGGCTGCCTAACCCAAGATTTTGATTGGGTGCCTACACGAGGTTTGAAGAATTTTACTCGGGGAGATTTCGCACAAAATTTTTATAGACATTCTAGTAAAGCTTTTAGACTGCACACGAAAAGTTATTTCGACCATGTGCATACTGTTATAATTCAGAAACATTTTGTAGTTGTAGGAGCTAGATGGATATTCTGCTGTGATACATTCTAAATTATTAAAATGTAAGGATTCTGTAGAAGATTTGATAGAATTGCATACAAGCTATTATGTTGTATCATGCTCTGAGAAAGGCATGCACTTTCTTCGGCATTTGAAGGTCTTTTTAAAGAGATGTTTCTCGACTCTGGAATTTAAACATTTCTTGACAGTATATGAATTATGTACATTTTCTTTCCTTTCTTTGCATAGAACTACTATATGGCGCCTAAGGGTCTATATGACTAGTTTTATGCGATGATTGTCGGTTTTTG

Ace file of the UniGene sp_v3.0_unigene458

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	RNA helicase n=1 Tax=Vigna radiata var. radiata RepID=Q9M6R6_VIGRR	-	-	0.0	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	39%
Sma3	DEAD-box ATP-dependent RNA helicase 7	-	-	2.235e-11	-

Source	ECs	Term	Type	e value	Identity
Sma3	Hydrolases, Acting on acid anhydrides, In phosphorous-containing anhydrides.	EC:3.6.1.-	-	1.421e-13	-

Source	KEGGs	Term	e value	Identity
Sma3	Purine metabolism	00230	1.421e-13	%
Sma3	Lipopolysaccharide biosynthesis	00540	1.421e-13	%
Sma3	Folate biosynthesis	00790	1.421e-13	%
Sma3	Metabolic pathways	01100	1.421e-13	%

Source	Gene names
Sma3	At3g22310; At3g22330; At5g62190; CHLREDRAFT_111269; CHLREDRAFT_119793; CHLREDRAFT_129561; CHLREDRAFT_196585; GSVIVT00018949001; GSVIVT00038510001; LOC_Os07g05050; LOC_Os09g34910; LOC_Os12g41715; MCB17.17; MCB17.3; MCB17.5; MICPUCDRAFT_39273; MICPUCDRAFT_5

Source	GOs	Term	Type	Identity
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	nucleolus	GO:0005730	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	nucleic acid binding	GO:0003676	Molecular Function	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	helicase activity	GO:0004386	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	ATP-dependent helicase activity	GO:0008026	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	response to cold	GO:0009409	Biological Process	-
Sma3	response to water deprivation	GO:0009414	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3	GUCT	IPR012562	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3	RNA helicase, DEAD-box type, Q motif	IPR014014	-	-
Sma3		IPR014021	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G62190.1	PRH75 DEAD box RNA helicase (PRH75) chr5:24980542-24983879 REVERSE LENGTH=671	63%
RefSeq	Arabidopsis thaliana	NP_201025.1	DEAD-box ATP-dependent RNA helicase 7 [Arabidopsis thaliana]	63%
RefSeq	Populus trichocarpa	XP_002318863.1	predicted protein [Populus trichocarpa]	68%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKW8

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 61 aas, putative chimeric sequence, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 695 - 802

Fln protein: MPALEITHNSSPSSDTKKKMKHVGSSGEKKHKKKKDRQGEEEEVDHAMNGSESMTSKRKLSKEAVGDDSSPKRSKKKKKTGGSDVEEEEEEEKEEKEENPNAVSNFNISEPLRQKLKSKGIESLFPIQAMTFEIIFNGSDMVGRARTGQGKTLAFVLPIVESLTNGPNAKGRNTAYGRAPSVIVLVPTRELAKQVHADFEVYGGAVGLHSSSVYGGAPYGPQEYALRRGVDIIVGTPGRIKDHIERGTLNLKSLKFRVLDEADEMLNMGFVEHVETILGKVEDVSKVQTLLFSATMPDWVKKIASRFLKPDRKTADLVGDDKMKASRSVRHIVLPCFYSARTQVIPDVIRCYSSGGSVIVFTETKNDASELAGSLQNARALHGDIQQSQREVILAGFRSGKFPLLVATDVAARGLDINDVQLIIQCEPPRDVETYIHRSGRTGRAGNTGVSVMLYDRKKQYMISQIERQAGVKFERVSAPQPGEVAKATGSTAAESITSVSDSVLPIFRQSAEDLVKKSDLSPVDIIAKALAKIAGYTEFKRRSLLSSQEGFATLILKTGRTMHTPSYAFTILRRVIPEETIAGIKGMSLTADGTGAVFDVANEHVDLFLAAQENADGVDIELIESLPEMQQVRNDQSRGHGGGGYGNGYGRGGRGGYSSGSRGGGGGSRGGRGGSSRGGRGGGSRGGRGWGRQ*

Protein Length: 696

Fln nts: TATTTTGAGCTACCCTATTTTAGGGTTTGAGGTTTAGAAGAGTTTGTGGAAACTAGGGTTTTGGTGTAGAGTTGTTTTTGTTAAAGCGCAATTCAATAGGAGATACATCGCGAAAAACCTTCAAGTTGTTTCTTCTTCTGAAACCTAGTCGTTCCATCGAGCTGAAGCGGGAGAAAGGAGT_-_ATGCCCGCTTTAGAAATCACCCACAACTCATCTCCTTCTTCTGATACGAAGAAGAAGATGAAGCACGTCGGCTCGTCTGGCGAGAAGAAGCACAAGAAGAAGAAGGATAGGCAGGGCGAGGAAGAGGAGGTGGACCATGCCATGAACGGAAGTGAATCTATGACCTCCAAGAGGAAATTGTCCAAGGAGGCCGTAGGTGATGATTCCAGTCCCAAGCGCAGCAAGAAGAAGAAGAAGACAGGGGGCAGCGATGTCGAAGAGGAAGAAGAAGAAGAGAAGGAAGAGAAGGAAGAGAATCCCAACGCGGTTTCTAATTTCAATATTTCGGAGCCACTGAGGCAGAAGCTCAAGTCCAAGGGGATTGAGTCACTCTTCCCCATCCAGGCAATGACCTTCGAAATTATCTTCAATGGCTCGGACATGGTCGGTCGAGCTCGTACAGGACAGGGTAAAACATTGGCATTTGTGTTGCCTATCGTGGAGTCATTAACAAATGGTCCGAATGCCAAAGGTAGGAATACAGCCTATGGAAGGGCCCCCAGTGTGATTGTGTTAGTTCCAACAAGGGAGCTTGCAAAACAGGTCCATGCTGACTTTGAGGTTTATGGTGGAGCTGTTGGCCTTCATTCATCCTCTGTATATGGAGGTGCTCCCTATGGTCCACAGGAATATGCATTGCGTAGAGGTGTGGATATCATTGTCGGGACACCTGGGCGCATTAAGGATCATATTGAAAGAGGGACATTGAATCTGAAATCCTTGAAATTTCGTGTCTTGGATGAGGCTGATGAAATGCTGAACATGGGCTTTGTAGAGCATGTTGAGACCATTCTTGGAAAAGTTGAGGATGTAAGCAAGGTTCAGACACTTCTTTTTAGTGCAACAATGCCAGATTGGGTAAAGAAGATTGCATCAAGGTTCTTGAAACCTGACAGAAAAACTGCAGATCTTGTGGGAGATGATAAGATGAAAGCTAGTAGAAGTGTGAGACACATCGTTCTTCCTTGTTTTTATTCAGCAAGAACACAAGTTATACCAGATGTTATTCGCTGCTATAGCAGTGGGGGAAGTGTGATTGTTTTTACTGAGACAAAGAATGATGCATCTGAACTCGCTGGATCTTTGCAAAATGCTCGTGCTTTGCATGGGGATATACAACAATCACAACGTGAAGTGATTCTTGCTGGATTCCGTTCGGGAAAGTTTCCACTCCTAGTTGCAACAGATGTTGCGGCACGCGGTTTGGACATCAATGATGTTCAGCTTATAATTCAGTGTGAACCACCAAGAGATGTGGAAACATACATTCATCGATCTGGGCGGACTGGTAGAGCAGGCAACACTGGAGTCTCTGTGATGCTTTATGATAGAAAGAAGCAATATATGATTTCTCAGATAGAAAGGCAGGCTGGTGTAAAATTTGAGCGTGTATCTGCTCCTCAGCCAGGAGAGGTTGCTAAAGCTACTGGATCAACTGCTGCAGAAAGTATTACTAGTGTCAGTGACAGTGTGCTTCCCATTTTTCGGCAATCAGCTGAGGATCTGGTGAAGAAATCTGATCTTTCACCTGTGGATATCATTGCAAAAGCACTAGCAAAGATTGCAGGATACACAGAATTCAAGAGAAGATCATTACTGTCCTCTCAAGAGGGTTTTGCAACCTTAATTCTTAAAACTGGAAGGACTATGCATACCCCATCATATGCTTTTACCATACTAAGAAGAGTTATACCAGAGGAAACTATTGCTGGGATCAAGGGCATGTCTCTCACTGCAGATGGAACTGGTGCCGTATTTGATGTGGCAAATGAGCATGTGGATTTATTTCTTGCAGCTCAGGAAAATGCAGATGGGGTTGATATCGAATTAATTGAAAGTCTACCAGAGATGCAACAAGTTCGTAATGACCAATCAAGAGGACATGGAGGGGGTGGCTACGGTAATGGGTATGGCAGAGGAGGGCGGGGAGGATATTCCAGTGGTTCAAGAGGAGGGGGTGGTGGTTCAAGAGGAGGCCGTGGTGGGAGTTCAAGAGGAGGTCGTGGTGGTGGTTCAAGAGGAGGTCGAGGTTGGGGACGTCAGTAAGGGAATTGGTGATCATCATTTCAAAAATTTCAAATAAAGTAATGTGTATTTTAACTGGCAGAGATTCTTAACTATAGCCTGGGCTGTCCGTCATATCCTTCTTGGGCAAAAAAGAGCTACAATTAGAGAAGCCTTGGTTTATTTGTTTAGGGGTACTGTAGTGTGAAAACATTCATTAGGGCAAGGTGTATGAGTATTCAAGTCACTTGCTTATATCTTGATCTGATTATCTCTAAGACCATCATCATCCATTAGATGATGCTTTATTTTTGTACTGTTTTTCTATATGAGAAAAGAAGATGGAGCCTTCGAATATGCTTCTACACGTTTCTGCTCGAAAGCTCCCAAGCTCTGTGCTTGTTTTTGGGTCATCTTTTTTCGACCCATATAGAATTATTTTACTCCACAAGGAGTTATTTAAGGTCCTTTAGACAGGCATGAGATTTCAAGGAGGCTGCCTAACCCAAGATTTTGATTGGGTGCCTACACGAGGTTTGAAGAATTTTACTCGGGGAGATTTCGCACAAAATTTTTATAGACATTCTAGTAAAGCTTTTAGACTGCACACGAAAAGTTATTTCGACCATGTGCATACTGTTATAATTCAGAAACATTTTGTAGTTGTAGGAGCTAGATGGATATTCTGCTGTGATACATTCTAAATTATTAAAATGTAAGGATTCTGTAGAAGATTTGATAGAATTGCATACAAGCTATTATGTTGTATCATGCTCTGAGAAAGGCATGCACTTTCTTCGGCATTTGAAGGTCTTTTTAAAGAGATGTTTCTCGACTCTGGAATTTAAACATTTCTTGACAGTATATGAATTATGTACATTTTCTTTCCTTTCTTTGCATAGAACTACTATATGGCGCCTAAGGGTCTATATGACTAGTTTTATGCGATGATTGTCGGTTTTTG

Fln Alignment:

Contig458___VSNFNISEPLRQKLKSKGIESLFPIQAMTFEIIFNGSDMVGRARTGQGKTLAFVLPIVESLTNGPNAKGRNTAYGRAPSVIVLVPTRELAKQVHADFEVYGGAVGLHSSSVYGGAPYGPQEYALRRGVDIIVGTPGRIKDHIERGTLNLKSLKFRVLDEADEMLNMGFVEHVETILGKVEDVSKVQTLLFSATMPDWVKKIASRFLKPDRKTADLVGDDKMKASRSVRHIVLPCFYSARTQ--VIPDVIRCYSSGGSVIVFTETKNDASELAGSLQNA---RALHGDIQQSQREVILAGFRSGKFPLLVATDVAARGLDINDVQLIIQCEPPRDVETYIHRSGRTGRAGNTGVSVMLYDRKKQYMISQIERQAGVKFERVSAPQPGEVAKATGSTAAESITSVSDSVLPIFRQSAEDLVKKSDLSPVDIIAKALAKIAGYTE-FKRRSLLSSQEGFATL------ILKTGRTMHTPSYAFTILRRVIP--EETIAGIKGMSLTADGTGAVFDVANEHVDLFLAAQENADGVDIELIESLPEMQ
B8LKW8_____________LSGLNLSQELVEALANRGITHLFPIQRAVLVPALEGRDLIGRAKTGTGKTLAFGIPIIERLTKDNKENKLLRQSRRLPRVLVLAPTRELAKQVEKEFKE--SAPFLSTACIYGGVSYVSQQNALARGVDVVVGTPGRIIDLVNSKSLKLNEVQFLVLDEADQMLAVGFEEDVEIILDNLP--AERQSMLFSATMPGWVKKLSRKHLN-NPMTIDLVGENEEKLAEGIKLYAVATTTSSNSKRSILGDLITVFAKGGKTIVFTQTKRDADEISLGLSSTIACEALHGDISQHQRERTLAGFREGKFSALVATDVAARGLDITNVDLVIHYEFPNDPETFVHRSGRTGRAGKEGIAILMYPSNGKRKVRSLEHEVGCRFEYINPPRQEDVLETSTQHVAKALGSVHPELINIFLPTANKLMEEKG---TNALAAALARLSGFSQPPSSRSLITHEEGWTTLQMTRESTMPDGRQQLNARELMGILSEMWPAAADKVGKIRTYG-DGKAEGAVFDLPKD-VAKELLLKQALPGYTVSKITQLPVME

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
422			40	60	1.0
423			75	25	0.996473
926		88		11	0.923564
1571	14			85	0.999981
1572		14		85	0.999981
1573	14			85	0.999981
1574		85		14	0.999981
2206	12		87		0.992554
2208			87	12	0.992554
2209	85	14			0.993533
2213		84	15		0.999986
2329	14		85		0.999981
2449	10		90		0.90298
2450		90		10	0.90298
2451	90		10		0.90298
2452	80		20		0.999996
2610	12	87			0.992554
2612		87	12		0.992554
2613		87	12		0.992554
2899		94	5		0.998578

SSRs (tot: 3)

Start position	End position	Sequence	Length
275	286	AAG AAG AAG AAG	12
404	419	AAG AAG AAG AAG AAG A	16
443	456	GAA GAA GAA GAA GA	14

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain