SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene12251

Length: 1939 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene12251 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=A9NUI7_PICSI	-	-	0.0	40%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	40%
Sma3	Pectinesterase	-	-	1.758e-16	-

Source	ECs	Term	Type	e value	Identity
Sma3	Pectinesterase.	EC:3.1.1.11	-	3.052e-20	-

Source	KEGGs	Term	e value	Identity
Sma3	Pentose and glucuronate interconversions	00040	3.052e-20	%
Sma3	Starch and sucrose metabolism	00500	3.052e-20	%
Sma3	Metabolic pathways	01100	3.052e-20	%

Source	Gene names
Sma3	ARATH36; At3g60730; GSVIVT00028033001; GSVIVT00035359001; H0315A08.12; OSJNBa0006B20.19; Os04g0438400; OsI_15995; OsJ_14902; PHYPADRAFT_190734; PME36; POPTRDRAFT_799193; RCOM_0708460; T4C21.140;

Source	GOs	Term	Type	Identity
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	enzyme inhibitor activity	GO:0004857	Molecular Function	-
Sma3	pectinesterase activity	GO:0030599	Molecular Function	-
Sma3	aspartyl esterase activity	GO:0045330	Molecular Function	-
Sma3	cell wall modification	GO:0042545	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Pectinesterase inhibitor	IPR006501	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NUI7

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 22 aas, W1: There is no M at the beginning, your sequence is shorter than subject: 475 - 559

Fln protein: GKSEDGRRECNVEVENIHQRELEEASKVSACFDQWEVQSVEGEIAKANLDLADSEQGLSKSFRISVIIGSVLIMIAVILAITVFLPHSSKKKHARNLIESDNWDQLSPVISIACEATQSPDVCRSSLITRGICKADIGEIIIATMDLSSEGSHKIHLLLGRLLQGASAAGNMKSKNAILNCLEFLEASTRWLTKSRATPLYNVNNIPVIKTWLGAVLTYQTDCQSALKHIPGRSKVVRKAVKQTDWVIILTSNALRMVDALGTYGPNISSWKPPLNLEGSRASSDKKWLWLWLSLLHKSIPTANEIVPNLTVSKDGSSLYSTVQAAVDRAPDYCSARFIIYIRAGVYEEIVRIPPTKFNLMLLGDGIGQTVITGNRSAQDLGGSIYSSTTIGVNGDGFVARDITFENSATGKGRGSYGVAVRVDSDLSTFKNCAFRGHHYSLYAHALRHLYTDCTIEGSLLNTAATLLQNCTNL*

Protein Length: 476

Fln nts: ACCAGATGATGATAGGTAAATGCTCGGCCACTGGGATATCACAGGTCGAGAATTTTATGAAAGGCTTGTGGAGATGAGATTCAAATCAGGTCTCAAGGATTATGCGCAGAAAATCAATAGCTCCTGTGAGTGTAAAAATTAGATTAGGGGAAAAGCGAAGATGGGCGGAGAGAATGTAATGTAGAAGTAGAGAATATTCATCAACGTGAGCTAGAAGAAGCATCCAAAGTGAGTGCTTGCTTCGATCAATGGGAAGTTCAGTCTGTGGAAGGCGAGATCGCAAAGGCAAATCTTGATCTTGCAGATAGTGAACAGGGTTTGAGTAAAAGCTTCAGAATTTCAGTAATTATAGGCTCAGTACTGATCATGATAGCAGTCATCCTTGCCATCACGGTCTTTCTACCCCACAGCTCTAAAAAGAAGCATGCCAGGAATCTAATTGAGAGCGACAATTGGGACCAACTATCTCCAGTAATCAGTATTGCATGTGAGGCCACACAGTCCCCAGATGTCTGCAGATCGTCTCTGATTACGAGGGGCATTTGTAAGGCAGATATTGGTGAGATTATAATTGCAACAATGGATTTGTCTTCAGAGGGATCCCATAAAATTCATTTGTTATTGGGAAGACTATTGCAGGGTGCCTCTGCAGCAGGTAATATGAAGTCAAAAAATGCAATTCTAAATTGCTTGGAGTTTTTAGAGGCTTCCACTAGATGGTTGACCAAGAGCAGAGCTACTCCTCTGTACAATGTGAATAATATTCCAGTAATTAAAACATGGCTAGGCGCTGTGCTCACTTACCAGACTGACTGTCAATCGGCATTGAAACACATTCCAGGCAGAAGCAAAGTGGTGAGAAAAGCCGTAAAACAAACAGATTGGGTCATCATTTTAACTAGCAATGCCCTAAGGATGGTTGATGCATTGGGCACTTATGGGCCTAATATCAGTTCCTGGAAACCTCCACTGAATTTAGAGGGATCAAGAGCTTCATCTGACAAGAAATGGCTATGGCTATGGCTATCCCTTCTGCATAAAAGTATTCCAACGGCGAATGAAATTGTTCCTAATCTAACAGTTTCTAAGGATGGCTCCTCCCTATATTCAACAGTTCAGGCTGCTGTGGACAGAGCACCAGACTATTGTTCTGCGAGGTTCATAATCTATATCAGAGCAGGGGTGTATGAAGAGATTGTTAGGATTCCTCCCACTAAGTTCAACTTGATGCTTCTGGGAGATGGCATCGGTCAGACTGTCATCACTGGGAATCGATCTGCGCAGGATCTTGGTGGAAGTATATACAGTTCCACAACTATCGGAGTGAATGGCGATGGATTTGTGGCACGGGACATAACTTTCGAGAACAGCGCCACAGGGAAAGGAAGAGGAAGTTATGGCGTAGCTGTACGAGTAGACAGTGATCTGTCAACATTCAAGAATTGCGCATTCCGTGGACACCACTACAGTCTCTACGCACATGCGCTACGTCACTTGTATACAGACTGCACAATTGAGGGTTCTCTCCTTAACACTGCTGCCACACTTCTCCAGAACTGCACCAACCTGTAGCTGGTCGTTCCAGTTTTGCGTCCTAGGAGATACCCTGGTGCCCTCAAATTTTCTCTCTCTATGTCTTTGTACACAGCCCAAAGCTTCGTGCAAGGCAATTCATAGCATTGTGTTTTTTTACCCAACTAGGTTTCAATGTTTGATGGAAGAATGATTGGTAGAATGCTATCACCCGTACTTGATGAAAGAACAATCAATTTACTTAACTGTAAAATTCTATCATGGCCATCTTTATTATCACCAACAAATAAACATTACAACAACAGATAGTATATCGCCACAGTTCTGTACTCTATAAAGAATACTGTTTATTGATAGTTACCTTACCTGAATCCTGGTTGCCGTAAATCGAGGTTACATCATACGA

Fln Alignment:

Contig12251___GLSKSFRISVIIGSVLIMIAVILAITVFLPH----SSKKKHARNLIESDNWDQLSPVISIACEATQSPDVCRSSLITRGICKADIG--EIIIATMDLSSEGSHKIHLLLGRLLQGASAAGNMKSKNAILNCLEFLEASTRWLTKSRATPLYNVNNIPVIKTWLGAVLTYQTDCQSALKHIPGRSKVVRKAVKQTDWVIILTSNALRMVDALGTYGPNISSWKPPLNLEGSRAXXXXXXXXXXXXXXXXSIPTANEIVPNLTVSKDGSSLYSTVQAAVDRAPDYCSARFIIYIRAGVYEEIVRIPPTKFNLMLLGDGIGQTVITGNRSAQDLGG--SIYSSTTIGVNGDGFVARDITFENSATGKGRGSYGVAVRVDSDLSTFKNCAFRGHHYSLYAHALRHLYTDCTIEGS---LLNTAATLLQNC
A9NUI7_______________GFTMLLRINIV--ATLLLITILLALCAQFSSANYLSAGSGHKKHLVKKSS---ASHSIDLACQASQYPDLCKSSLQANSNISENAGAEEIIGAAMVLSSDKTTQSYLHSKQLL---NTSDNRNLTGAVKDCLEFLEGSIRYIAKSRTQQL-NPRNIKDVKIWMSAALSHQYDCSSALKYV-NTTQMVGRSMQELVIVMNFTSNALSMVDALDTYGKDMVIWRPPKTERSSKLSSTADYSHHYNKIWD--VLEVDDLVSDVTVSKDESSM--SIQQAVNSAPDYSERRFVIRIKAGVYEEIVRIPPTKTNLMFVGDGMDRTVITGSMRVPSLPGVPSTYDSATVAVNADGFLARDIAFENAAGPVSQ--QAVALRVDSDLSAFYNCALLGHQDTLYTHTLRQFYRNCRIEGTVDFIFGNSAAIFENC

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
742	87	12			0.992554
744	87	12			0.992554
814	90	10			0.90298
817			90	10	0.90298
820			88	11	0.923564
822		88		11	0.923564
825	88			11	0.923564
829	11		88		0.923564
838	50		50		1.0
857	87		12		0.992554
1053		20	80		0.995493
1055		80		20	0.995493
1056		80		20	0.995493
1229	25		75		0.996473
1396	66		33		0.997452
1459	33			66	0.997452
1699	16	83			0.994512
1700		16	83		0.994512
1702	83			16	0.994512
1703			83	16	0.994512
1748	66		33		0.997452
1761	66			33	0.997452

SSRs (tot: 1)

Start position	End position	Sequence	Length
913	932	ATAGCC ATAGCC ATAGCC AT	20

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain