SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene7219

Length: 1416 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene7219

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	chloroplast polyprotein of elongation factor Ts precursor [Arabidopsis thaliana]	-	-	0.0	43%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	38%
Sma3	Elongation factor Ts	-	-	4.088e-07	-

Source	Gene names
Sma3	AT4g29060; At4g29060; F19B15.90; GSVIVT00016702001; PHYPADRAFT_129127; PHYPADRAFT_164715; POPTRDRAFT_261946; RCOM_1051260;

Source	GOs	Term	Type	Identity
Sma3	intracellular	GO:0005622	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	translation elongation factor activity	GO:0003746	Molecular Function	-
Sma3	translational elongation	GO:0006414	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Ubiquitin-associated/translation elongation factor EF1B, N-terminal	IPR000449	-	-
Sma3	Translation elongation factor EFTs/EF1B	IPR001816	-	-
Sma3	Ribosomal protein S1, RNA-binding domain	IPR003029	-	-
Sma3	Nucleic acid-binding, OB-fold	IPR012340	-	-
Sma3	Translation elongation factor EFTs/EF1B, dimerisation	IPR014039	-	-
Sma3	Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote	IPR015940	-	-
Sma3	Translation elongation factor Ts, conserved site	IPR018101	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NXR9

Fln msg: STOP codon was not found. Distance to subject end: 167 aas, your sequence is longer than subject: 471 - 381

Fln protein: CILSLRSFSINFSPEVRRRGCDLDHSLISYRLSASVGNEVVMQESDVETKQATGEGGVSDGPSEGEKSVEIGSLSEGESVAAASSRTAGQSKRTGIIRKNENPRVQNEELVPGAIFSGKVRAIQPFGAFIDLGASTDGLVHISRLSDSFVKQVSDVVSIGQEVTVRVLEANIETGRISLTMKANDDRNQPRGEKTSAPSSGISSGKVVRTGKVAGRFSQKKADETQKRSKFQKGQTLEGKVKNIIRSGAFISLPDGEEGFLRSSEVSEGYGNLDGNMDLQIGQDVTVRVLRIEKGRVNLTMKREEDVAGLNMQLNQGEIEVASNPFELAFRKNPVISAFLKEQETLKKSDKSEKPLVEAEEQNYDKQVKAPVGTIDSEEEVVSNEKAGYEIAEKEMTEIPENTEDKGSGTTETKDGLTNGNAEKIDESVRLGTEQILKSEISDIQGDEEKVLDSTAAEMENVLANDSAYLH

Protein Length: 472

Fln nts: AATGTATATTATCATTGAGATCATTTTCAATTAATTTCTCTCCTGAGGTCAGAAGAAGAGGTTGTGACTTAGATCACAGCTTAATATCATATAGACTTTCAGCATCGGTAGGGAATGAAGTGGTAATGCAGGAATCTGATGTTGAGACTAAGCAGGCTACTGGAGAAGGTGGAGTGTCTGATGGTCCATCAGAAGGTGAGAAATCAGTAGAAATTGGTTCATTATCAGAAGGTGAATCTGTTGCTGCAGCATCTTCTCGAACTGCAGGGCAGTCCAAACGAACTGGTATTATCAGGAAGAATGAGAATCCACGAGTACAAAATGAGGAATTGGTTCCTGGTGCGATTTTCTCAGGTAAAGTAAGAGCAATTCAACCATTTGGTGCATTTATTGATTTGGGAGCGTCAACAGATGGACTAGTACATATTTCCAGGCTCAGTGACTCCTTTGTGAAACAAGTAAGCGATGTGGTTTCCATTGGGCAGGAGGTTACTGTCAGGGTATTAGAAGCAAACATAGAAACGGGACGTATTTCTTTGACAATGAAAGCAAATGATGATCGGAACCAGCCAAGAGGAGAGAAAACTTCTGCTCCTAGTTCTGGCATAAGCAGTGGTAAGGTTGTTCGAACTGGAAAGGTTGCTGGAAGATTCAGCCAGAAGAAGGCAGACGAGACTCAGAAGAGATCCAAGTTCCAAAAGGGACAAACTCTAGAAGGAAAAGTTAAGAATATAATCAGATCTGGAGCATTTATATCACTTCCTGATGGTGAAGAGGGTTTTCTTCGCTCATCTGAAGTATCTGAGGGCTATGGAAATCTTGATGGAAATATGGATCTACAAATTGGGCAAGATGTTACTGTCCGAGTTTTGCGTATTGAGAAGGGAAGGGTGAATTTGACTATGAAAAGAGAGGAAGACGTTGCGGGTCTGAATATGCAACTTAACCAAGGTGAGATTGAAGTTGCTTCCAATCCTTTTGAATTGGCTTTCCGTAAAAATCCTGTTATTTCTGCTTTCTTAAAAGAACAGGAGACTCTGAAGAAATCTGATAAATCAGAAAAACCTCTGGTAGAGGCTGAAGAACAAAATTATGATAAACAGGTAAAAGCACCTGTTGGCACAATTGACTCTGAGGAGGAAGTTGTATCCAATGAGAAAGCTGGTTATGAAATTGCAGAGAAGGAGATGACTGAAATACCAGAAAACACAGAGGATAAAGGCTCTGGAACTACTGAAACTAAGGATGGATTAACAAATGGAAATGCAGAGAAAATAGATGAATCAGTTAGATTAGGAACTGAACAAATACTAAAAAGCGAAATCAGTGACATACAAGGGGATGAAGAGAAGGTTTTGGATTCTACAGCAGCAGAAATGGAGAATGTCTTGGCAAATGATTCTGCATATCTACATT

Fln Alignment:

Contig7219___GAIFSGKVRAIQPFGAFIDL-----GASTDGLVHISRLSDSFVKQVSDVVSIGQEVTVRVLEANIETGRISLTMK
A9NXR9______________GDVFEGRVSSVTKFGAFVNLRFPDGGYYVDGLVHISEVSWDLVRDVRDILKKGDDVRVKVIQINVEKSRLAFSIK

SNPs (tot: 28)

Position	A %	C %	G %	T %	Probability
468	25			75	0.996473
469	25		75		0.996473
472			75	25	0.996473
516		80		20	0.995493
572		60		40	1.0
587	20	80			0.995493
634	14		85		0.993533
635		14	85		0.993533
637	85			14	0.993533
638	85			14	0.993533
780		16		83	0.994512
800		12		87	0.992554
801	87		12		0.992554
806	87			12	0.992554
808	12		87		0.992554
809		12	87		0.992554
816	87	12			0.992554
817	87			12	0.992554
818	87		12		0.992554
821		12		87	0.992554
823		12	87		0.992554
861		12		87	0.992554
862	12		87		0.992554
864		87		12	0.992554
931	20			80	0.995493
934			60	40	1.0
963	75		25		0.996473
1195		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain