SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene26515

Length: 1812 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene26515

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	EST1075749 Normalized pine embryo library, Lib_D Pinus taeda cDNA clone PWAB656 3' end, mRNA sequence.	-	-	97%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	s-adenosylmethionine-dependent methyltransferase domain-containing protein	-	-	69%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACF2

Fln msg: Separated hits, possible frame ERROR between 1332 and 1334,

Fln protein: MGKHGKKQHGRSNSRLKADDDPLPSSAYDSEPPQQQHPLTADEFPLEEEEEKHEKYKYEEQSSCDLPSKFVLYQRSVQSPKGDISYMQKFFWTYVGGRVPLHLREDFCGTALISAEWLRSDSRRTAVGVDLDIEALTWGLENNVQKVGADAYSRIYLFRGNVLHSLTSAKCIKSALLGSENYKSLRETEDISGSVIDDDEADLVKKLEISEHVRDYCKRKESSFPGADIVCAFNYSCCCLQSRSELVLYFKQAWNAISEKGGIFVMDLYGGTSSEHALKLRRRYEDFMYVWEQEDFNIINRTTKISLHFYLNKNQRSLRHAFTYHWRLWTLPEIKDCLEEAGFNSVHFWMREMPNIQEKMEVEDYEMNDNVxxHEEVSSFNQKDAWNAYIVGIANKI*

Protein Length: 399

Fln nts: TCGTGACCTTGGCTGTCACTCAACGTCATAGTCTGTGACATTCGTTGTAATTTTGTAGCATGCAATTTCTCAAGTAAGGAACAATTTTGAAAACCTAAAATTTCGTATCATTCAATTTCGAAGTTCACATGTAAAACTTCTCGTCTTAATTCGAATGACCACTCATGAAAATCGTTCTGCATCATGAAAGTTAGTATGTGAATGCAGTGCAGCTGA_-_ATGGGAAAGCATGGAAAGAAACAGCATGGCCGTAGTAATTCTCGTTTGAAGGCAGATGATGATCCATTGCCTTCCTCTGCTTATGACTCTGAACCACCACAACAGCAACACCCTTTAACCGCTGATGAGTTTCCTCTCGAGGAAGAAGAAGAGAAACACGAAAAATATAAATATGAAGAGCAGTCTTCGTGCGACTTACCATCCAAGTTTGTACTCTATCAGCGTTCTGTGCAGTCTCCTAAGGGAGATATTAGCTACATGCAAAAGTTTTTTTGGACCTATGTGGGAGGACGTGTGCCTCTTCACCTGCGGGAGGATTTTTGTGGAACTGCTTTAATAAGTGCAGAGTGGTTACGTAGTGATTCTCGACGAACAGCTGTAGGAGTTGATTTGGATATTGAAGCACTTACATGGGGCCTGGAAAACAATGTTCAAAAAGTTGGAGCTGATGCCTACTCTAGAATATATCTATTTCGTGGGAATGTCTTGCATTCACTGACCAGTGCAAAATGTATCAAGTCTGCTTTGTTGGGTTCAGAAAACTACAAATCATTGAGAGAAACTGAGGATATTTCTGGCTCTGTAATAGATGATGATGAGGCCGATCTTGTTAAAAAGCTCGAAATCAGTGAACATGTACGAGATTATTGTAAACGAAAAGAGAGTTCATTTCCAGGAGCAGATATTGTATGTGCATTTAACTACAGTTGTTGTTGCCTTCAAAGTCGTTCAGAGTTGGTATTGTATTTTAAGCAAGCTTGGAATGCAATATCAGAGAAGGGTGGAATCTTTGTAATGGATCTCTATGGAGGCACATCATCAGAGCATGCTCTAAAACTCCGCAGAAGATATGAGGATTTTATGTATGTATGGGAGCAAGAGGATTTCAATATCATTAATAGAACAACAAAAATTAGTCTACATTTTTATCTCAATAAAAATCAGCGCAGTCTTCGGCATGCTTTTACTTATCACTGGCGTCTATGGACCTTACCAGAAATCAAAGATTGCTTAGAAGAGGCTGGATTCAATTCTGTGCACTTTTGGATGCGTGAAATGCCAAATATACAAGAAAAGATGGAAGTGGAGGACTATGAAATGAATGATAATGTCAAxxxTCATGAAGAAGTTTCTAGCTTCAATCAGAAGGATGCTTGGAATGCCTACATTGTTGGAATTGCAAATAAAATTTAG___AAACGGAGCTGAAGTTTGGCAGATATTTCTTCATTTCAACATTTTAAACGTTAATGTTTTTGTCTGTGTCTTGAGTTCTTTGGGTGCACTGTCAACAGCAATGAAAAAACTCAAGTTTTGCTCGTACTTTGTCTAATTAAAGAAGTTGATTATCTTTGAACAGTTTGATTTTGACAGGAAAACCTCCATGTTGGACATCTCTATATATACTTACTATAGGCAGCAGTTGTTTCTACATTCCTTAATTTCCTTTTCAGATTTTGATTGTGAATGATCAAGCACCATATACTATGTCTTTAAATTTCCATTATGCTTTGTTTAGTTGTGTTGGAGGCAACTGATCGGACTTTGTGGCACACCAAAAATGAACAATGAAGTCGATATTTTGGGAGTTATTTTACTTT

Fln Alignment:

Contig26515___MGKHGKKQHGRSNSRLKADDDPLPSSAYDSEPPQQQHPLTADEFPLXXXXXXXXXXXXXXQSSCDLPSKFVLYQRSVQSPKGDISYMQKFFWTYVGGRVPLHLREDFCGTALISAEWLRSDSRRTAVGVDLDIEALTWGLENNVQKVGADAYSRIYLFRGNVLHSLTSAKCIKSALLGSENYKSLRETEDISGSVIDDDEADLVKKLEISEHVRDYCKRKESSFPGADIVCAFNYSCCCLQSRSELVLYFKQAWNAISEKGGIFVMDLYGGTSSEHALKLRRRYEDFMYVWEQEDFNIINRTTKISLHFYLNKNQRSLRHAFTYHWRLWTLPEIKDCLEEAGFNSVHFWMREMPNIQEKMEVEDYEMNDNVKxHEEVSSFNQKDAWNAYIVGIANKI*KRS
D5ACF2_______________MGKQGKKHHGRSHSRLREDDDPLPSSAYDSEPPQQQHPLTIDEFPLEEEEKEAKYKYEE-QSSCDLPSKFVLYQRSVQSPKGDISYMQKFFWTYVGGRLPLHLREDFCGTALISAEWLRSDSRRTAVGVDLDIEALTWGLENNVQQVGADAYSRICLFHGNVLHSLTNAKCIKSASLGSENYKSLRETEDNSGSVTDDDEADLIKKLEVSENVQDYCGQKESSFPAADIVCAFNYSCCCLQSRSELVLYFKQAWSAISKKGGIFVMDLYGGTSSEHALKLRRRYEDFMYVWEQEDFNIINRTTKISLHFYLSKNQRSLRYAFSYHWRLWTLPEIKDCLEEAGFNSVHFWMREMPNIQERREVEEFEVNDNVKxYEEISSFNQKDAWNAYIVGIANKVEKRS

SNPs (tot: 71)

Position	A %	C %	G %	T %	Probability
159	50		50		1.0
160		50	50		1.0
161		50	50		1.0
174		85	14		0.993533
328		87		12	0.992554
480	77			22	0.999999
484	14		85		0.993533
526			77	22	0.999999
529	11		88		0.923564
859	85		14		0.993533
870	42		57		1.0
873	16		50		1.0
970		10	90		0.90298
973	90			10	0.90298
974	90		10		0.90298
978			10	90	0.90298
979			90	10	0.90298
980		10	90		0.90298
982	10	20			0.999998
983	88			11	0.923564
984		11		88	0.923564
985	11		77	11	0.968132
1101		11	88		0.923564
1205	25		75		0.996473
1206			75	25	0.996473
1207	75			25	0.996473
1260	66		33		0.997452
1264			33	66	0.997452
1265		33		66	0.997452
1266	33		66		0.997452
1268	66			33	0.997452
1270	33		66		0.997452
1320		25		75	0.996473
1322	75			25	0.996473
1323	75	25			0.996473
1328		25		75	0.996473
1329	75	25			0.996473
1330	75			25	0.996473
1333			25	75	0.996473
1335	75			25	0.996473
1341		25		75	0.996473
1342			75	25	0.996473
1343	75	25			0.996473
1422			83	16	0.994512
1423	83	16			0.994512
1429	83	16			0.994512
1430		16	83		0.994512
1432		16		83	0.994512
1437	83			16	0.994512
1439	83			16	0.994512
1440		16		83	0.994512
1442		16		83	0.994512
1446	16			83	0.994512
1457	85		14		0.993533
1459		14		85	0.993533
1460			14	85	0.993533
1462	85		14		0.993533
1464	85	14			0.993533
1466			85	14	0.993533
1468			14	85	0.993533
1469	85		14		0.993533
1470	85	14			0.993533
1474		14		85	0.993533
1475	14			85	0.993533
1476		14		85	0.993533
1527		30		70	1.0
1539		75		25	1.0
1545	25			75	1.0
1552	75		25		1.0
1659		12		87	0.992554
1711		80		20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain