SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene25931

Length: 1952 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene25931
GATGTGAACAAAGTTAAGGAAGCGACCAAACAGTTTAAGCTTGGGGATAGTTTGCCTCTTCCTGGCAGCAGAACACGATCATCTTCTGGAGGATCTGGAGGATCACAAGATCTCTCAATCATTTTAGCTCAACCTTCAGCCTTAGTTCCTCAAAATGGACCCTCGACTGTACCAGCACCAGAACCAACGGCAATGATGATGGGCATGGGGTGTCAGCTGGACCAATTCCAGAGGATTTCTTCCAAAATACAATATCTTCCTTCCAGGCTGCAGCATCTCTGCCTCCTGTTGGGGTATACAATCAGTCTCAAAATCAGACCTTTCAGGGTACCCCCAGTAGTGGACAACCTCCTAATTATCAAGGGGTGCCAACTGATTTTGGTCTCTTTGGTAGTGGTGTACCTCCCCAAGTGTCCCAGTCTAGTGGAATCAGTTCTGGCCTGTCAATGGATGGTACACCTGTTCAAGTTTTGGGCCTTCCTGATGGGGGCATTCCTCCACACGGGCAAGTTACTCCAGTGTCAACCTCATCATCAGCTATTGATCTTGGTTTATTTGAAACACCTACTTCAGCATTAGCGAGTAAGAATAATGCCGAAACTCCAGTGCTTGCTTCTGCAAAAGGTGGTACAACGAATCTTGGGCGTCCCAACTCACCACCAATTGCTGTACGACCGGGACAGGTGCCTCGTGGAGCTGCAGCATCAAAGTGCTTTAAAGCTGGACTTGCTCATCTTGAACAAAACCAACTTTCAGATGCGATGTCCTGTCTGGATGAGGCTTTCTTAGCATTGGCAAAAAGATCAGTCACTAGGAGTTGACATCAGAGCTCAAGCCTCGATTTGTGCACAGTACAAGATTGCTGTCTTACTACTTCAGGAAATTTCTCGCCTACAGCGTGTGCAAGGGCCTAGTGCTGTCAGTGCGAAAGAGGAAATGGCCAGACTTTCTCGCCATCTTGGCTCTTTGCCTCTTCTTGCAAAGCACCGAATAAGTTGCATCCGAACTGCTATTAAAAGGAATATGGATGTGCAGAACTATGCATTTGCAAAATCTGACCTTGATCTTCTCCTCTCAAAGGCCCCTCCTAACAAACAGGATGAACTCAGAAGCTTGATCAATGTATGCACACAAAGAGGTCTCACTGATAAATCCATTGATCCGACAGAGGATGCCTCACAGTTTTGTTCTGCAACACTTGGCCGCTTGCCAACAATAGGACATGATGTCTGTGATCTATGTGGTGCAAAATTTTCAGCTCTTTCATCACCAGGCTGTATAATATGTGGTATGGGAAGTATTAAAAGATCCGATGCAGTGGCAGGCCCAGTTCCATCACCATTTGGTTAAGAGGCAGGAAGAGCTAGTTTGCCAGCTAAAGAGTGAAGATAGTGAATTCTTCATGCTACATTTTCGAGCGAAAGAGAAACATTTCATGGGGAAATCAATTATGACATATGGAGGCAATGTTTTCTTGTAAAGAAAAATGAGTGTAGGGTTGAGCAGAGCAAAGACTTGGAAAAGTGTGTGATTATACTAGTTTTTCCCCTTTGATAAACACCTTACAAGAGAATAAATGGTAGCTCCTGGTATTTATGGTTTTCTTCCAACAGAAATGTAGGTGGCACAAGTTAGAAATGGCATTTGTGCCCAGTTAACAATAGAGGAAATGATTTTATCTGACTCCAGCTTTATTCATGAATATTTTGGTAGGCCAACAGAAGCCTTGAAAAAATAGAAATGCAGCAAATGGACTTACAATAGTTGACATGATTGTGAAAGAGTCTGTGAGGCTATATCCACTAGTAATTGCTTTGATTGTAAGAACTAACAAAGCTACACTGCTTGGAAAGCTTAATTTTCCACAAGAGATGGAGTTAATGATTGCAATCTTAGCCATTCACCATGATTAGGCCTTGTGGGTGATGATGTGAATGAATTCAATATCCAAA

Ace file of the UniGene sp_v2.0_unigene25931 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	p450 domain containing protein	-	-	6.0e-06	26%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	39%
Blast2go	vascular protein	-	-	0.0	88%

Source	GOs	Term	Type	e value	Identity
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	88%

Source	InterPros	Term	Type	Identity
Sma3	WD40 repeat	IPR001680	-	-
Sma3	WD40/YVTN repeat-like-containing domain	IPR015943	-	-
Sma3	WD40-repeat-containing domain	IPR017986	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-
Sma3		IPR019782	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5ABC4

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 81 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 61 - 181

Fln protein: KNRNAANGLTIVDMIVKESVRLYPLVIALIVRTNKATLLGKLNFPQEMELMIAILAIHHD*

Protein Length: 62

Fln nts: GATGTGAACAAAGTTAAGGAAGCGACCAAACAGTTTAAGCTTGGGGATAGTTTGCCTCTTCCTGGCAGCAGAACACGATCATCTTCTGGAGGATCTGGAGGATCACAAGATCTCTCAATCATTTTAGCTCAACCTTCAGCCTTAGTTCCTCAAAATGGACCCTCGACTGTACCAGCACCAGAACCAACGGCAATGATGATGGGCATGGGGTGTCAGCTGGACCAATTCCAGAGGATTTCTTCCAAAATACAATATCTTCCTTCCAGGCTGCAGCATCTCTGCCTCCTGTTGGGGTATACAATCAGTCTCAAAATCAGACCTTTCAGGGTACCCCCAGTAGTGGACAACCTCCTAATTATCAAGGGGTGCCAACTGATTTTGGTCTCTTTGGTAGTGGTGTACCTCCCCAAGTGTCCCAGTCTAGTGGAATCAGTTCTGGCCTGTCAATGGATGGTACACCTGTTCAAGTTTTGGGCCTTCCTGATGGGGGCATTCCTCCACACGGGCAAGTTACTCCAGTGTCAACCTCATCATCAGCTATTGATCTTGGTTTATTTGAAACACCTACTTCAGCATTAGCGAGTAAGAATAATGCCGAAACTCCAGTGCTTGCTTCTGCAAAAGGTGGTACAACGAATCTTGGGCGTCCCAACTCACCACCAATTGCTGTACGACCGGGACAGGTGCCTCGTGGAGCTGCAGCATCAAAGTGCTTTAAAGCTGGACTTGCTCATCTTGAACAAAACCAACTTTCAGATGCGATGTCCTGTCTGGATGAGGCTTTCTTAGCATTGGCAAAAAGATCAGTCACTAGGAGTTGACATCAGAGCTCAAGCCTCGATTTGTGCACAGTACAAGATTGCTGTCTTACTACTTCAGGAAATTTCTCGCCTACAGCGTGTGCAAGGGCCTAGTGCTGTCAGTGCGAAAGAGGAAATGGCCAGACTTTCTCGCCATCTTGGCTCTTTGCCTCTTCTTGCAAAGCACCGAATAAGTTGCATCCGAACTGCTATTAAAAGGAATATGGATGTGCAGAACTATGCATTTGCAAAATCTGACCTTGATCTTCTCCTCTCAAAGGCCCCTCCTAACAAACAGGATGAACTCAGAAGCTTGATCAATGTATGCACACAAAGAGGTCTCACTGATAAATCCATTGATCCGACAGAGGATGCCTCACAGTTTTGTTCTGCAACACTTGGCCGCTTGCCAACAATAGGACATGATGTCTGTGATCTATGTGGTGCAAAATTTTCAGCTCTTTCATCACCAGGCTGTATAATATGTGGTATGGGAAGTATTAAAAGATCCGATGCAGTGGCAGGCCCAGTTCCATCACCATTTGGTTAAGAGGCAGGAAGAGCTAGTTTGCCAGCTAAAGAGTGAAGATAGTGAATTCTTCATGCTACATTTTCGAGCGAAAGAGAAACATTTCATGGGGAAATCAATTATGACATATGGAGGCAATGTTTTCTTGTAAAGAAAAATGAGTGTAGGGTTGAGCAGAGCAAAGACTTGGAAAAGTGTGTGATTATACTAGTTTTTCCCCTTTGATAAACACCTTACAAGAGAATAAATGGTAGCTCCTGGTATTTATGGTTTTCTTCCAACAGAAATGTAGGTGGCACAAGTTAGAAATGGCATTTGTGCCCAGTTAACAATAGAGGAAATGATTTTATCTGACTCCAGCTTTATTCATGAATATTTTGGTAGGCCAACAGAAGCCTTGAAAAAATAGAAATGCAGCAAATGGACTTACAATAGTTGACATGATTGTGAAAGAGTCTGTGAGGCTATATCCACTAGTAATTGCTTTGATTGTAAGAACTAACAAAGCTACACTGCTTGGAAAGCTTAATTTTCCACAAGAGATGGAGTTAATGATTGCAATCTTAGCCATTCACCATGATTAGGCCTTGTGGGTGATGATGTGAATGAATTCAATATCCAAA

Fln Alignment:

Contig25931___ILVGQQKP*KNRNAANGLTIVDMIVKESVRLYPLVIALIVRTNKATLLGKLNFPQEMELMIAILAIHHD*ALW
D5ABC4_______________ISICRHQPPKPENVSQ-LKIMGMVLNESLRLYPPVVALIRQAGQDMKLGNLSVPGGTQLLFPIIALHHDPSLW

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
668	85			14	0.999981
669			14	85	0.999981
671			14	85	0.999981
673	85		14		0.999981
681	14			85	0.999981
685		14	85		0.999981
687		14		85	0.999981
690	85	14			0.999981
691	78		14	7	0.999974
749			90	10	0.90298
826		87		12	0.992554
902		25		75	0.996473
903			25	75	0.996473
904		25	75		0.996473
905	25	75			0.996473
1447		75		25	1.0
1508			87	12	0.992554
1525			28	71	1.0
1528		71	28		1.0
1534	71	28			1.0
1536	28			71	1.0
1537	28		71		1.0
1540	71			28	1.0
1544			28	71	1.0
1545	28			71	1.0
1546		28	71		1.0

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain