SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene25904

Length: 2523 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene25904
ATGGGGGCGAAAGATCAAAAGCAGAGCTCGGCAGTTTTTAACATTTCTTGAGCATTGACCGGCATTGACTACTTGAATTTGTTGATCGGTAGCCGAGAAATGCACAAGGATTTAAGTAATAAGGATCAAACTTCCAATCTCCATCTGGGGGAAAACGAAGTTCAGATTGTAAGGAGTATATTGTGTAATCTTACGGATGACAGATAAATCAGTAGTTGTAGTGACCGTCTTCACTGTTTTTCAAGAGATTTAGTATATATTATTATTATTATTACCATTAACAAGGAAGAAAAAGTTTTCTCTGGAATCTGCAGGTTTCTGTAGCTCGTGCTAACATCCAAGATCTCTTCAGATTCAATCATATATTTCTAAAGACAAGTACCGGGTCGCGCCTACTGAAATGCAGAACCCGTTAATAGTTGAAACGAGCTGTGGCTCCTTACTGCAGGAGCTACAGCAAATATGGGACGAAGTAGGAGAAAGTGATGCGGAACGAGATAGGATGTTGCTTCAGTTGGAGCAAGAATGTTTAGATGTCTACAAGCGGAAAGTTGATCAAGCCACCCGCTCAAGGGCTCGATTACTTCAGACCCTGGCTGATTCCAGAGCTGAACTCACCAATCTTCTCTCTGCACTTGGCGAGCACTCTTTTCATGGCCTTCCTGAAAAGCCTACAGGCACCCTGAAGGAACAACTTGCTGCTATTACACCGGCTTTGGAACAGCTTTGGCAGCAAAAGGAAGAGAGGATCAAGCAATTTTTGGATGTTCAAGTACAAATTCAGAGGATTTGCGGAGAAATTGCTGGAAAATTGCAGCTCAATGAAGCTATAACTCCGAAAGTCGATGATCAGGACTTATCATTGAAAAAGTTTGATGAATTTCATCTACAGTTTCAAGAACTTCAAAGAGAAAAGAGTGATCGGCTCCACAAGGTTCTTGAGTACGTTAATACTGTACGTGATCTTTGCGCTGTATTGGGAATGGACTTTTTTAATATCATTACCGAAGTTCATCCTAGCTTAGGCGATTCGGTGGGGATTCAGTCAAAAAGTATTAGCAATGATACCTTGGCCAGGCTAGCCAGAACAGTGCAATCGTTAAAGGAGGAGAAGAAGCAAAGGTTGCAAAAGCTTCAAGAACTAGGGACTTCACTGACAGAATTGTGGAATCTAATGGATACCCCTATGGAAGAACAAAATTTATTTGATCATGTGACTTGCAACATTTCAGCATCTGTGGAGGAGGTCACGGTCCCAGGTGCACTTGCTATTGATATTATCGAACAGGCTGAGGTAGAAGTTGAAAGATTGGATCAGCTAAAGGCTAGTAAAATGAAAGAGCTTGTGCTTAAGAAGCAATTAGAGCTTGAAGAAATATATAGACGGGCCCACATGGAAGTAGATTCTGAGTCTGCCCAAGAGAAATTAATAGCTTTGATTAATTCTGGCAATGTCGATCCTTCGGAGCTGCTAGCTAGCATGGATGACCAGATCTCCAAAGCAAAAGAAGAGGCATTTAGTAGAAAGGAAATAATGGAGAAGGTGGAGAAATGGATGTCGGCTTGTGAAGAAGAAAGCTGGCTTGAAGATTACAGCAGGGATGATAATAGGTACAATTCCAGCAGGGGGGCTCACATTAATCTCAAACGTGCAGAACGAGCTCGAGTAATTGTTAACAAAATTCCTGCTCTTGTGGATTCCTTGATTGCAAAAACAAGGGCTTGGGAGGAGGAAAGGTCGACTACCTTTTTATATGACGCAGTACCCCTCCTGGCGATGTTAGATGAGTACACTATGTTAAGGCATGAAAGAGAAGACGAGAAACGGAGGCTCAGGGATCAAAAACGTTTTCATGAGCAGCTGACAACCGAGCAAGAAACATTGTTTGGTTCAAGGCCAAGTCCCAACAGGCCACAATCTTCAAAGAAGGGTGCAGGCCCCCGTGCAAATGGTAGTAGTTCAGGTGGCACTCCTTTGAATCGACGCCTTTCACTCGGTGTGCAGCAATCTGGAACAAATGGAGTCACACCTGTTAGGCAGGGCATGTCTTCCAGCAAGGAAGGTAAAAGAGAGTTGTCTCGGCCAGTGGCCCCTGTCAATTATGTTTCTCTTCCCAAAGAAGACTCTGTTTCACAAGTTTCTGAGAATGAGTCCACTCCTTCCACCCCATAACTGAATAATGGCGTTGACGATGCTATCTATTATAAAGTTAGCCGTAACTGTTGTTTCTGCTGAACTGACTTTGAAGATAAGAAGATAGGTCAGTATATATTATTGCTGAGAATGCTTGATAATCTATTTGAGATTATCAAGCACTGTTCACAGCTATTTCTCATATCGTGCTTAGAGACAACTACGTGTAAAACATATTTCATTATAGCTATTTCAAATAATGTTGTAGGTTACGGGTTTGGTTCATACCCCTTATTAAAATGACCATGAGTAGCATGTTTCCAGGCTAAACTTATTAACTTTAAGGTGTATAAGGTAAATTTAAGATTACTCTCATGTAATACAACATT

Ace file of the UniGene sp_v2.0_unigene25904 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Protein regulator of cytokinesis, putative n=1 Tax=Ricinus communis RepID=B9SYY2_RICCO	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	69%
Blast2go	microtubule-associated proteins 65-1	-	-	83%

Source	GOs	Term	Type	Identity
Blast2go	anaphase	GO:0051322	Biological Process	83%
Blast2go	microtubule bundle formation	GO:0001578	Biological Process	83%
Blast2go	cytokinesis	GO:0000910	Biological Process	83%
Blast2go	cortical microtubule organization	GO:0043622	Biological Process	83%
Blast2go	microtubule nucleation	GO:0007020	Biological Process	83%
Blast2go	positive regulation of microtubule polymerization	GO:0031116	Biological Process	83%
Blast2go	microtubule binding	GO:0008017	Molecular Function	83%
Blast2go	protein dimerization activity	GO:0046983	Molecular Function	83%
Blast2go	cortical microtubule, transverse to long axis	GO:0010005	Cellular Component	83%
Blast2go	preprophase band	GO:0009574	Cellular Component	83%
Blast2go	spindle	GO:0005819	Cellular Component	83%
Blast2go	phragmoplast	GO:0009524	Cellular Component	83%
Blast2go	nucleus	GO:0005634	Cellular Component	83%

Source	InterPros	Term	Type	Identity
Sma3	Domain of unknown function DUF296	IPR005175	-	-
Sma3	Microtubule-associated protein, MAP65/ASE1-type	IPR007145	-	-
Sma3	AT hook, DNA-binding motif	IPR017956	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AC28

Fln msg:

Fln protein: MQNPLIVETSCGSLLQELQQIWDEVGESDAERDRMLLQLEQECLDVYKRKVDQATRSRARLLQTLADSRAELTNLLSALGEHSFHGLPEKPTGTLKEQLAAITPALEQLWQQKEERIKQFLDVQVQIQRICGEIAGKLQLNEAITPKVDDQDLSLKKFDEFHLQFQELQREKSDRLHKVLEYVNTVRDLCAVLGMDFFNIITEVHPSLGDSVGIQSKSISNDTLARLARTVQSLKEEKKQRLQKLQELGTSLTELWNLMDTPMEEQNLFDHVTCNISASVEEVTVPGALAIDIIEQAEVEVERLDQLKASKMKELVLKKQLELEEIYRRAHMEVDSESAQEKLIALINSGNVDPSELLASMDDQISKAKEEAFSRKEIMEKVEKWMSACEEESWLEDYSRDDNRYNSSRGAHINLKRAERARVIVNKIPALVDSLIAKTRAWEEERSTTFLYDAVPLLAMLDEYTMLRHEREDEKRRLRDQKRFHEQLTTEQETLFGSRPSPNRPQSSKKGAGPRANGSSSGGTPLNRRLSLGVQQSGTNGVTPVRQGMSSSKEGKRELSRPVAPVNYVSLPKEDSVSQVSENESTPSTP*

Protein Length: 592

Fln nts: ATGGGGGCGAAAGATCAAAAGCAGAGCTCGGCAGTTTTTAACATTTCTTGAGCATTGACCGGCATTGACTACTTGAATTTGTTGATCGGTAGCCGAGAAATGCACAAGGATTTAAGTAATAAGGATCAAACTTCCAATCTCCATCTGGGGGAAAACGAAGTTCAGATTGTAAGGAGTATATTGTGTAATCTTACGGATGACAGATAAATCAGTAGTTGTAGTGACCGTCTTCACTGTTTTTCAAGAGATTTAGTATATATTATTATTATTATTACCATTAACAAGGAAGAAAAAGTTTTCTCTGGAATCTGCAGGTTTCTGTAGCTCGTGCTAACATCCAAGATCTCTTCAGATTCAATCATATATTTCTAAAGACAAGTACCGGGTCGCGCCTACTGAA_-_ATGCAGAACCCGTTAATAGTTGAAACGAGCTGTGGCTCCTTACTGCAGGAGCTACAGCAAATATGGGACGAAGTAGGAGAAAGTGATGCGGAACGAGATAGGATGTTGCTTCAGTTGGAGCAAGAATGTTTAGATGTCTACAAGCGGAAAGTTGATCAAGCCACCCGCTCAAGGGCTCGATTACTTCAGACCCTGGCTGATTCCAGAGCTGAACTCACCAATCTTCTCTCTGCACTTGGCGAGCACTCTTTTCATGGCCTTCCTGAAAAGCCTACAGGCACCCTGAAGGAACAACTTGCTGCTATTACACCGGCTTTGGAACAGCTTTGGCAGCAAAAGGAAGAGAGGATCAAGCAATTTTTGGATGTTCAAGTACAAATTCAGAGGATTTGCGGAGAAATTGCTGGAAAATTGCAGCTCAATGAAGCTATAACTCCGAAAGTCGATGATCAGGACTTATCATTGAAAAAGTTTGATGAATTTCATCTACAGTTTCAAGAACTTCAAAGAGAAAAGAGTGATCGGCTCCACAAGGTTCTTGAGTACGTTAATACTGTACGTGATCTTTGCGCTGTATTGGGAATGGACTTTTTTAATATCATTACCGAAGTTCATCCTAGCTTAGGCGATTCGGTGGGGATTCAGTCAAAAAGTATTAGCAATGATACCTTGGCCAGGCTAGCCAGAACAGTGCAATCGTTAAAGGAGGAGAAGAAGCAAAGGTTGCAAAAGCTTCAAGAACTAGGGACTTCACTGACAGAATTGTGGAATCTAATGGATACCCCTATGGAAGAACAAAATTTATTTGATCATGTGACTTGCAACATTTCAGCATCTGTGGAGGAGGTCACGGTCCCAGGTGCACTTGCTATTGATATTATCGAACAGGCTGAGGTAGAAGTTGAAAGATTGGATCAGCTAAAGGCTAGTAAAATGAAAGAGCTTGTGCTTAAGAAGCAATTAGAGCTTGAAGAAATATATAGACGGGCCCACATGGAAGTAGATTCTGAGTCTGCCCAAGAGAAATTAATAGCTTTGATTAATTCTGGCAATGTCGATCCTTCGGAGCTGCTAGCTAGCATGGATGACCAGATCTCCAAAGCAAAAGAAGAGGCATTTAGTAGAAAGGAAATAATGGAGAAGGTGGAGAAATGGATGTCGGCTTGTGAAGAAGAAAGCTGGCTTGAAGATTACAGCAGGGATGATAATAGGTACAATTCCAGCAGGGGGGCTCACATTAATCTCAAACGTGCAGAACGAGCTCGAGTAATTGTTAACAAAATTCCTGCTCTTGTGGATTCCTTGATTGCAAAAACAAGGGCTTGGGAGGAGGAAAGGTCGACTACCTTTTTATATGACGCAGTACCCCTCCTGGCGATGTTAGATGAGTACACTATGTTAAGGCATGAAAGAGAAGACGAGAAACGGAGGCTCAGGGATCAAAAACGTTTTCATGAGCAGCTGACAACCGAGCAAGAAACATTGTTTGGTTCAAGGCCAAGTCCCAACAGGCCACAATCTTCAAAGAAGGGTGCAGGCCCCCGTGCAAATGGTAGTAGTTCAGGTGGCACTCCTTTGAATCGACGCCTTTCACTCGGTGTGCAGCAATCTGGAACAAATGGAGTCACACCTGTTAGGCAGGGCATGTCTTCCAGCAAGGAAGGTAAAAGAGAGTTGTCTCGGCCAGTGGCCCCTGTCAATTATGTTTCTCTTCCCAAAGAAGACTCTGTTTCACAAGTTTCTGAGAATGAGTCCACTCCTTCCACCCCATAA___CTGAATAATGGCGTTGACGATGCTATCTATTATAAAGTTAGCCGTAACTGTTGTTTCTGCTGAACTGACTTTGAAGATAAGAAGATAGGTCAGTATATATTATTGCTGAGAATGCTTGATAATCTATTTGAGATTATCAAGCACTGTTCACAGCTATTTCTCATATCGTGCTTAGAGACAACTACGTGTAAAACATATTTCATTATAGCTATTTCAAATAATGTTGTAGGTTACGGGTTTGGTTCATACCCCTTATTAAAATGACCATGAGTAGCATGTTTCCAGGCTAAACTTATTAACTTTAAGGTGTATAAGGTAAATTTAAGATTACTCTCATGTAATACAACATT

Fln Alignment:

Contig25904___VAPTEMQNPLIVETSCGSLLQELQQIWDEVGESDAERDRMLLQLEQECLDVYKRKVDQATRSRARLLQTLADSRAELTNLLSALGEHSFHGLPEKPTGTLKEQLAAITPALEQLWQQKEERIKQFLDVQVQIQRICGEIAGKLQLNE-AITPKVDDQDLSLKKFDEFHLQFQELQREKSDRLHKVLEYVNTVRDLCAVLGMDFFNIITEVHPSLGDSVGIQSKSISNDTLARLARTVXXXXXXXXXXXXXXXXLGTSLTELWNLMDTPMEEQNLFDHVTCNISASVEEVTVPGALAIDIIEQAEVEVERLDQLKASXXXXXXXXXXXXXXEIYRRAHMEVDSESAQEKLIALINSGNVDPSELLASMDDQISKAKEEAFSRKEIMEKVEKWMSACEEESWLEDYSRDDNRYNSSRGAHINLKRAERARVIVNKIPALVDSLIAKTRAWEEERSTTFLYDAVPLLAMLDEYTMLRHEREDEKRRLRDQKRFHEQLTTEQETLFGSRPSPNRPQSSKKGAGPRANGSSSGGTPLNRRLSLGVQQSGTNGVTPVRQGMSSSKEGKRELSRPVAPVNYVSLPKEDSVSQVSENESTPSTP
D5AC28_______________MATKKLQRPMFSDTNCGSLLQDLELIWDEVGASDGEREKMLLQLEQECLEVYRRKVDQANLSRARLHQLLADSEAELEHLISALGERSFVGRPEKQTGTLKEQLAAIAPALGDLRKKKEERVKKFMDVQVQIQSICAEIAGNLNVNDLCTTPKVDEHDLSLKKLDEFHDQLQELHREKNDRLQKVFDYVNTVHALSAVMGLDFSKIVSEVHPSLDDSAGNQSKSISNDTLAKLAETVESLKEEKQRRLQKIQELGTTLIELWNLMDITMGEQKHFDHVTCYISASVDEVSMPRALASNIIDQAEVEVKRLDRLKAGKMKQLLHKKQTELEEIYRKAHMEIDTNSAQEKIMAMIDSGTINHSDLLASMDDQIAKAKEEALSRKEIMEKVDKWMAACEEESWLEDYNRDENRYSVSRGAHINLKRAERARVIVNKIPALVDSLMGKTRAWEEERGTTFFYDEVPLLAMLDEYNMMRQEREEEKLRLRDKKRLQGQLANEPEPIFVAKPTPHRQLSTKRSTGLRANGNSVDATPLNRRLSLGVQQTGMNGVNPARQGLSVQRDGKREHNRSMIPRNYFSLSKEDSASQPSANGSTVSSP

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
1985	25			75	0.996473
1987			75	25	0.996473
1988	75		25		0.996473
1989		75		25	0.996473
2214		88	11		0.923564
2220	12	87			0.992554
2223			87	12	0.992554
2224	80			20	0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
2250	2266	TAA TAA TAA TAA TAA TA	17

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain