SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene25872

Length: 2162 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene25872
ATAATGTATAGAAATAGCAAAAGAATAAGAGAGTAAGGAAGATCAACCGATCTTACTTCAACAGCGGCGGAGTGGCAAGAAGATCCCAGTGTCCACAACCACAACGAAAACAATGGCAGCAACTAGGACGTTGGGTACTTCTTCTTGCATAGCCTTCTCACACAACCCACAGTGCATTCCCATTCCCTTTCCCTGCAACATAAGCCTCTCCTCTAAAACCTCCTTCTGGAACCCAATACCCACTGCTCAGAAACATCCCCTCATGGCCAGAGAATCAGGGCGACGTAGAAATTCAAAGGTTGAGATTATTTGTCAGAAGAGAACCCATGGTAGATTTCAGAATGACAGGAACTCTCCTCATGCAGATGGTGGAAAAGGAGATAACAGTTGGTCTGAACTAACAATAGAGAAATCTCTGTTGAAGAATGCCTCCAATAACCTGAAAAATGAACAGGTTATTGATGCAGAAGATCTAAAAGATGTCCCTCTTCTTAATGCAGTTGTGAAGGTTTATTGCACTCATACTGAACCAGATTATTCCCTTCCTTGGCAAAAGCAAAGGCAGTATACGAGCACAGGCAGTGCTTTTATGATAGGTGGGGGAAAACTGTTGACAAATGCCCATTGTGTTGAATATCGTACACAGGTAAAAGTGAAGAGGAGAGGAGATGACACTAAATATGTTGCTACGGTTTTAGCCAAAGGTGTAGAGTGTGACATTGCTCTGCTTTCAGTTGAGAGTGAAGGCTTTTGGAAAGGGACTGAGCCTCTTCAATTTGGACGTCTGCCATGTCTTCAGGATGCAGTGACTGTTGTAGGTTATCCACTAGGAGGAGATACAATTTCAGTTACTAAGGGTGTCGTTTCACGTATTGAGGTGACATCATATGCACATGGAGCTGCTGATCTGTTGGGTATCCAAATTGATGCAGCAATAAATCCTGGAAACAGTGGGGGTCCAGCTTTTAATGATAGTGGAGAGTGCATTGGAATAGCATTTCAGGTTTATAGATCAGCAGATGCTGAGAATATTGGGTATGTGATTCCAACAACTGTTGTTTCTCATTTTCTAAATGATTATGAACGAAATGGGAAGTACACAGGATTTCCTTGTCTTGGCGTGTTACTTCAGAAATTGGAGAATCCAGCACTTAGGGCATGTCTGAATGTCACTGTGAATGAGGGTGTATTGATTCGAGCAGTTGAACCAACATCTCTTGCAAAGGAAGTTTTGAAGAAGGGAGATGTCATTGTAAGCTTTGATGGTGTACCAGTGGCTAATGAAGGAACTGTACCTTTCCGTTCCACAGAGCGTATTGCATTTGGATTTCTCATCAGTCAAAAATATACAGGTGATACAGCAGAGCTTGGGATTATTCGAGAAGGAAAGAAAATGAATGTTGTAACAACATTGAAGCCCCGTGTTCATCTGGTACCATATCACATTGAAGGAGGGCAACCTTCATACCTAATTGTTGCAGGATTAGTGTTTACACCTCTATCAGAGTCACTAATAGACGATGAATGTGAAGAATCTATGGGGTTAAAACTCATGGCTAAAGCTCGGTACTCTTTGCCAAAATTTGAAGGGGAACAGATGGTTATCTTATCACAGGTTTTGGCCAGTGATGTTAATATTGGATATGAAGACATGAGCAATCAACAGGTCCTGAAGCTTAATGAGATCAAAATTAAAAATATAAGGCATCTAGCTCATATTGTGGATTCATGCAATGATAAATACCTGATTTTCGAGTTGGAGGATAACTTTCTTGTAGTCATGGAACGACAAGCAGCCTCAGCAGCAACCCCTCAGATCTTGAAGGACTATTGCATTGCATGTGAAAGGTCCCCTGATTTATTGGAGCCCTATGTGAATTCGTCAGAAATTGTTAATGAAGTAAATGAGAACTTGGACGGTAGCCCTGTATTAAATTCTGAAATTGATTTTGATGGGCTTCTTTGGGCATGAGTTGTATATTTATCTGTAAAATATTTCATATATTCAATGTCATTATCTCGCAATCATTTCAATTCATCTTTGTGGTGATAAATGCTAGTTTGAGCCTTTGCTCAAGCAAATAAGTCAATTCAATAGTTGAAAATTGCGTTTCTGATTCTTGATTCTTTAGAAGTTCTCCTCATATCATAGGTTCGAGAGG

Ace file of the UniGene sp_v2.0_unigene25872 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	protease Do-like 2 [Arabidopsis thaliana] sp\|O82261.2\|DEGP2_ARATH RecName: Full=Protease Do-like 2, chloroplastic; Flags: Precursor gb\|AAG41447.1\|AF326865_1 putative DegP2 protease [Arabidopsis thaliana] gb\|AAK14061.1\|AF245171_1 DegP2 protease [Arabidopsi	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	protease do-like chloroplastic-like	-	-	79%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on peptide bonds (peptide hydrolases), Serine endopeptidases.	EC:3.4.21.-	-	0.0	79%

Source	GOs	Term	Type	Identity
Blast2go	photosystem II repair	GO:0010206	Biological Process	79%
Blast2go	serine-type endopeptidase activity	GO:0004252	Molecular Function	79%
Blast2go	chloroplast stromal thylakoid	GO:0009533	Cellular Component	79%
Blast2go	chloroplast thylakoid membrane	GO:0009535	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	HMG-I/HMG-Y, DNA-binding, conserved site	IPR000637	-	-
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Peptidase S1/S6, chymotrypsin/Hap	IPR001254	-	-
Sma3	PDZ domain	IPR001478	-	-
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Peptidase S1C, HrtA/DegP2/Q/S	IPR001940	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Peptidase S1B, glutamyl endopeptidase I	IPR008256	-	-
Sma3	Serine endopeptidase DegP2	IPR015724	-	-
Sma3	AT hook, DNA-binding motif	IPR017956	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NMT7

Fln msg: your sequence is longer than subject: 620 - 192

Fln protein: MAATRTLGTSSCIAFSHNPQCIPIPFPCNISLSSKTSFWNPIPTAQKHPLMARESGRRRNSKVEIICQKRTHGRFQNDRNSPHADGGKGDNSWSELTIEKSLLKNASNNLKNEQVIDAEDLKDVPLLNAVVKVYCTHTEPDYSLPWQKQRQYTSTGSAFMIGGGKLLTNAHCVEYRTQVKVKRRGDDTKYVATVLAKGVECDIALLSVESEGFWKGTEPLQFGRLPCLQDAVTVVGYPLGGDTISVTKGVVSRIEVTSYAHGAADLLGIQIDAAINPGNSGGPAFNDSGECIGIAFQVYRSADAENIGYVIPTTVVSHFLNDYERNGKYTGFPCLGVLLQKLENPALRACLNVTVNEGVLIRAVEPTSLAKEVLKKGDVIVSFDGVPVANEGTVPFRSTERIAFGFLISQKYTGDTAELGIIREGKKMNVVTTLKPRVHLVPYHIEGGQPSYLIVAGLVFTPLSESLIDDECEESMGLKLMAKARYSLPKFEGEQMVILSQVLASDVNIGYEDMSNQQVLKLNEIKIKNIRHLAHIVDSCNDKYLIFELEDNFLVVMERQAASAATPQILKDYCIACERSPDLLEPYVNSSEIVNEVNENLDGSPVLNSEIDFDGLLWA*

Protein Length: 621

Fln nts: ATAATGTATAGAAATAGCAAAAGAATAAGAGAGTAAGGAAGATCAACCGATCTTACTTCAACAGCGGCGGAGTGGCAAGAAGATCCCAGTGTCCACAACCACAACGAAAACA_-_ATGGCAGCAACTAGGACGTTGGGTACTTCTTCTTGCATAGCCTTCTCACACAACCCACAGTGCATTCCCATTCCCTTTCCCTGCAACATAAGCCTCTCCTCTAAAACCTCCTTCTGGAACCCAATACCCACTGCTCAGAAACATCCCCTCATGGCCAGAGAATCAGGGCGACGTAGAAATTCAAAGGTTGAGATTATTTGTCAGAAGAGAACCCATGGTAGATTTCAGAATGACAGGAACTCTCCTCATGCAGATGGTGGAAAAGGAGATAACAGTTGGTCTGAACTAACAATAGAGAAATCTCTGTTGAAGAATGCCTCCAATAACCTGAAAAATGAACAGGTTATTGATGCAGAAGATCTAAAAGATGTCCCTCTTCTTAATGCAGTTGTGAAGGTTTATTGCACTCATACTGAACCAGATTATTCCCTTCCTTGGCAAAAGCAAAGGCAGTATACGAGCACAGGCAGTGCTTTTATGATAGGTGGGGGAAAACTGTTGACAAATGCCCATTGTGTTGAATATCGTACACAGGTAAAAGTGAAGAGGAGAGGAGATGACACTAAATATGTTGCTACGGTTTTAGCCAAAGGTGTAGAGTGTGACATTGCTCTGCTTTCAGTTGAGAGTGAAGGCTTTTGGAAAGGGACTGAGCCTCTTCAATTTGGACGTCTGCCATGTCTTCAGGATGCAGTGACTGTTGTAGGTTATCCACTAGGAGGAGATACAATTTCAGTTACTAAGGGTGTCGTTTCACGTATTGAGGTGACATCATATGCACATGGAGCTGCTGATCTGTTGGGTATCCAAATTGATGCAGCAATAAATCCTGGAAACAGTGGGGGTCCAGCTTTTAATGATAGTGGAGAGTGCATTGGAATAGCATTTCAGGTTTATAGATCAGCAGATGCTGAGAATATTGGGTATGTGATTCCAACAACTGTTGTTTCTCATTTTCTAAATGATTATGAACGAAATGGGAAGTACACAGGATTTCCTTGTCTTGGCGTGTTACTTCAGAAATTGGAGAATCCAGCACTTAGGGCATGTCTGAATGTCACTGTGAATGAGGGTGTATTGATTCGAGCAGTTGAACCAACATCTCTTGCAAAGGAAGTTTTGAAGAAGGGAGATGTCATTGTAAGCTTTGATGGTGTACCAGTGGCTAATGAAGGAACTGTACCTTTCCGTTCCACAGAGCGTATTGCATTTGGATTTCTCATCAGTCAAAAATATACAGGTGATACAGCAGAGCTTGGGATTATTCGAGAAGGAAAGAAAATGAATGTTGTAACAACATTGAAGCCCCGTGTTCATCTGGTACCATATCACATTGAAGGAGGGCAACCTTCATACCTAATTGTTGCAGGATTAGTGTTTACACCTCTATCAGAGTCACTAATAGACGATGAATGTGAAGAATCTATGGGGTTAAAACTCATGGCTAAAGCTCGGTACTCTTTGCCAAAATTTGAAGGGGAACAGATGGTTATCTTATCACAGGTTTTGGCCAGTGATGTTAATATTGGATATGAAGACATGAGCAATCAACAGGTCCTGAAGCTTAATGAGATCAAAATTAAAAATATAAGGCATCTAGCTCATATTGTGGATTCATGCAATGATAAATACCTGATTTTCGAGTTGGAGGATAACTTTCTTGTAGTCATGGAACGACAAGCAGCCTCAGCAGCAACCCCTCAGATCTTGAAGGACTATTGCATTGCATGTGAAAGGTCCCCTGATTTATTGGAGCCCTATGTGAATTCGTCAGAAATTGTTAATGAAGTAAATGAGAACTTGGACGGTAGCCCTGTATTAAATTCTGAAATTGATTTTGATGGGCTTCTTTGGGCATGA___GTTGTATATTTATCTGTAAAATATTTCATATATTCAATGTCATTATCTCGCAATCATTTCAATTCATCTTTGTGGTGATAAATGCTAGTTTGAGCCTTTGCTCAAGCAAATAAGTCAATTCAATAGTTGAAAATTGCGTTTCTGATTCTTGATTCTTTAGAAGTTCTCCTCATATCATAGGTTCGAGAGG

Fln Alignment:

Contig25872___MNVVTTLKPRVHLVPYHIEGGQPSYLIVAGLVFTPLSESLIDDECEESMGLKLMAKARYSLPKFEGEQMVILSQVLASDVNIGYEDMSNQQVLKLNEIKIKNIRHLAHIVDSCNDKYLIFELEDNFLVVMERQAASAATPQILKDYCIACERSPDLLEPYXXXXXXXXXXXXNLDGSPVLNSEIDFDGLLWA
A9NMT7_______________MNVLTTLKPRVHLVPYHIEGGQPSYLIVAGLVFTPLSESLIEDECEESMGLKLMAKARYSLPKFEGEQMVILSQVLANDVNIGYEDMSNQQVLKLNEIKIKNIRHLAHIVDSCNDKYLIFELEDNFLVVMERQAASAETPQILKDYGIACERSPDLSEPYVNSSEIVNEVNENLDGSPVLNSEIDFDGLLWA

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
875			75	25	0.996473
1303			50	50	1.0
1336		20		80	0.995493
1337	80	20			0.995493
1338		20	80		0.995493
1345	75	25			0.996473
1346	25	75			0.996473
1347		75		25	0.996473
1359	33	66			0.997452
1365		66		33	0.997452
1526		66		33	0.997452
2111	62	37			1.0
2112	62	37			1.0
2113		37	62		1.0
2126		20		80	0.995493
2129	20	80			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain