SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene24442

Length: 1498 nt

Origin of reads: Inia v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene24442

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=D5AB84_PICSI	-	-	71%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	71%
Blast2go	probable pectinesterase pectinesterase inhibitor 51-like	-	-	78%

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Metallophosphoesterase domain	IPR004843	-	-
Sma3	Pectinesterase inhibitor	IPR006501	-	-
Sma3	Carbohydrate-binding/sugar hydrolysis domain	IPR006633	-	-
Sma3	Phosphate-induced protein 1	IPR006766	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AB84

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 261 - 302

Fln protein: MLYANSGGSFWYIFYSFLVPNSARGGSATVGVNADGFVARDIAFENTAGPEMHQAVALRVDSDLSAFERCAFLGHQDTLYAHALRQFYRNCRIEGNVDFIFGNAAAIFHNCSILVRSRQVPSNLSEANPITAHGRLDPGQTTGFVFENCSIDGTEEYMAEFYGNPKMHKAYLGRPWKAYSRTIFMRSYLGPVIQPDGWLPWDGTFALDTLYYGEFENYGPGAGNVSARVPWSSEIPPGAVGMYSIQNFIEGDLWLPQIHM*

Protein Length: 262

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGGTGGGAGTGCTGTGTATATGGTACTTACATCTGAGGATGTTGTAGTGGAAGGATTCTGCATGAGTTCTTGTGGATTCCATGCCAATCTCAATCTATTATCGCATCAACAGTCGATAAAAAGCCAAATGATGGGGGAGGGGAAGCAGCAACAACAACTCCTTCCATACGCGTGGGTTGGGAACTCTGCAAGCCAGTGCCCTGGACTGTGCGCGTGGCCCTTCCATCAGCCCATCTACGGACCCCAGACTGCCCCTCTGGTGGCACCGAACGGTGACATGGGCATCGATGGGATGATCATCAATATTGCCAATGTTGTTGCAGGAGCAGTCACCAATCCCTTTAACACCGGCTACTTCCAAGGTGACGCAGGTGCACCACTAGAGGCTGTGTCCGCATGCCCGGGAATATATGGAAAGGGGGCATATCCTGGGTTCCCTGGGGAGCTTTTGGTAGATGAATCCACGGGGTCAAGCTACAATGCCCATGGAATCAATGGGCGACAGTTTCTACTGCCCGCAATGTGGGATCCTGCTACCAGATCTTGCAAGGCATTGGTATAACCATTTTTTCGGTTTTGTGCTAATTCTAG_-_ATGCTCTATGCAAACAGTGGAGGAAGCTTCTGGTATATATTTTATTCTTTCCTCGTGCCGAATTCGGCACGAGGCGGCTCTGCAACTGTCGGGGTGAACGCGGACGGTTTTGTGGCGCGGGACATTGCGTTCGAGAATACTGCGGGGCCGGAGATGCATCAGGCTGTCGCCCTCAGAGTCGATAGCGATCTCTCAGCATTCGAGAGGTGCGCCTTTCTCGGACACCAGGACACCCTATACGCGCACGCCCTCCGCCAGTTTTATCGGAATTGCAGGATCGAAGGCAACGTCGACTTCATATTTGGAAACGCAGCGGCCATCTTCCACAACTGCTCCATTCTCGTCCGCTCTCGCCAGGTGCCGTCTAACCTTTCCGAAGCGAACCCCATAACTGCCCACGGGCGATTGGATCCGGGTCAGACTACTGGCTTCGTATTCGAGAACTGCAGCATCGATGGTACGGAGGAATACATGGCAGAATTCTACGGCAATCCGAAAATGCACAAAGCCTACCTGGGTCGGCCGTGGAAAGCGTATTCACGGACCATTTTCATGCGGTCCTACCTGGGGCCAGTTATTCAGCCGGACGGGTGGCTGCCTTGGGATGGAACCTTTGCCCTGGACACGCTGTACTATGGAGAGTTTGAAAATTACGGGCCGGGTGCCGGCAATGTCTCTGCCAGAGTGCCATGGTCTAGTGAGATTCCTCCAGGAGCAGTGGGTATGTATTCTATCCAGAACTTCATCGAGGGAGACCTGTGGCTACCCCAGATTCATATGTGATTCCCATTTAGCTTGCTGTCTGTCAGCACCACCCTCTGAATCTTCAATATAGTAGTAATATAATGCTGCAAAAGACCATCTCTGGAAACTATTCATTGTAA

Fln Alignment:

Contig24442___GSATVGVNADGFVARDIAFENTAGPEMHQAVALRVDSDLSAFERCAFLGHQDTLYAHALRQFYRNCRIEGNVDFIFGNAAAIFHNCSILVRSRQVPSNLSEANPITAHGRLDPGQTTGFVFENCSIDGTEEYMAEFYGNPKMHKAYLGRPWKAYSRTIFMRSYLGPVIQPDGWLPWDGTFALDTLYYGEFENYGPGAGNVSARVPWSSEIPPGAVGMYSIQNFIEGDLWLP
D5AB84_______________GSATVAVNADGFVARDITFENAAGPGSQQAVALRVDSDLSAFYSCAFLGHQDTLYTHTLRQFYRNCRIEGTVDFIFGNSAAIFDNCLILVRPRQINSNKGSSNPVTAQGRTDPAEPTGFVFHNCTINGTEEYTRQFYANPKIYKAYLGRPWKMYSRAIIINSYLGELISPEGWMPWIGDFALDTLYYGEYQNFGPGA-KVSGRMPWSNQIPEINVGMYSARSFIQGDEWLP

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
300	12		87		0.992554
303	87		12		0.992554
304		87	12		0.992554
305	57	42			1.0
306	14			85	0.993533
307			57	42	1.0
308		14	85		0.993533
310	14	85			0.993533
316			14	85	0.993533
317		28	71		1.0
318	42		57		1.0
319		28	57		1.0
320	57			42	1.0
321		28		57	1.0
322	28		71		1.0
391		22		77	0.999999
647		37		62	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain