SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene23863

Length: 1980 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

>sp_v2.0_unigene23863
TTGATCCCATACTCGGGCAACGGGACGCGTGTAGCCCGATTTTAATTCAGAAAAAGTCACCTACTCTGCAAGCAGGGGTTGCAATGTATTCTAGTAATGAATCTTGCTCTTCAGATGGAAAAAAGTGGCACATTTTATTGAGTGGCATTTTCAGATTCATTCCAGATAATTCAACAAGTTTGTTGGAGAATAAATGTGACAGCTACCTTTTGTTTCATTTTGAAACTAATGTTGGGATTGGCACCCGGTCATTTCTATATGTTTTGGGCCTTGCTTATTGTTTTATTGGATTGTCTGCCATAACTGCTTGCTTCTTTCGTTCAATGGAGAATGTTGTGAAACAGACAAGGGAGATTATTTATGTGGATCCTGTAACGGGTGCTAAAAGAGTCAGGAGAGAAAGGATATGGAACTATACATTGGCAGATATTAGTTTATTAGCTCTTGGGACAAGCTTTCCACAGATATCACTTTCTACGATTGACGCTTTTCAGAACCTTGGTCAACGATATGCTGGGGGTTTGGGTCCTGCAACACTCGTGGGTTCTGCTGCATTTGACCTCTACCCTATCCATGCCATTTGTGTAGTAGTTCCAAGAGCAGGCACAGTGAAAAAAATCTCAGATTTAGGAGTATGGTTGGTTGAACTTACATGGTCTTTTTGGGCATATGTTTGGCTTTACATAATAATAGAGGAAAATGTTGTGGGGAAACCCAAGGTTGCTTCCCGACATTCATGGATGACATCCTTAGTAAACTACTTGTTGTACACTGGTGTGCCTAATCCAGGGGGATTCAATCCAGGTGTGGACTCCGAATGTGGTGACTATCTGGGAGGCTGGATTGACTATAACACAGTTTGGCTTACTAATGTTACATGCATATGGACAAGACAGAGGGTGGCGCTGTGTTGCGATTCCCTTGCCAAGACAAGAGAGGCCAGCAGCTTGGGTGCCTGTTGATACTACATATGGTTGCAGCCAAGATGGAAATCATGAAAAGTATTCTGAGTCTGCTTTATCTGACTGCATGCCTTCAGATTCTGCTGTTGATATCTTCTCAATACACAATGATTCCATATCTGAGGAAGTACCTGGGAGGGAAAATGTTGAATCTTCCTTTCTACCCTGGGATCCTGATGACATCATTGGAACTGAAAGAAAGGATATGTTCTTGGTTTGGCGTCAACAGTTCCACAATGTTCTCACTCTGGAAGATCGTAAAACAAGAACGAATAGTAAGGCTCCATTGCGAGTAATGAGAATAGTTTGGCAATTGATAATTCTACCATGGAGGTTTTTATTTGCCTTCGTCCCCCCATGTGATATTGCACATGGATGGGTAGCTTTCAGTGTTTCGCTAATTTTTATAACTGGCATTTCCTATGTTCTTACAAAGCTTACAGATTTGATCAGCTGTGTGACAGGAGTGAATCCATATATCATAGCTTTCACAGCCCTTGCAAGCGGAACCTCATGGCCTGATCTAGTTGCAAGTAAAATAGCGGCAGAACGCCAAGAGACAGCAGATTCTGCTATTGCAAACATCACATGCAGATGTTTGGTGCACCTTATGTTGGTTTTGCTGATGGTGCGTACCGCAGTGCCCGCAACTTGTCTTCTGCGGCATGGGTGATCTACGATCCTCATGGTGAGTTGATAGACCTCCAAGGTGTTTGTCTAGGTTGTACCACAAACAATGTCGCTGAATATAGTGTTGTTATTGAGTTACCGGCTGAGGCGATCAATCTCGGTATTCGTAATTGGTTGTGAACCTGGACTCTCAGCTTGTCGTCCACCAGATCAATGGGCGATTTTTTGTCAGAGACCATCGGATTTTAAGGTTGTATTTACGTGTTAAATTGTTAGAAAGGAATTTTGATTTTATCACATATCAGTATATTCCACGAAGATTAAACACGTTAACTGATGCATTAGCTAATCATGTATTAGACAAACATTTGCTTAACATGTAATCAAA

Ace file of the UniGene sp_v2.0_unigene23863

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	magnesium/proton exchanger [Arabidopsis thaliana] gb\|AAF14229.1\|AF109178_1 magnesium/proton exchanger AtMHX [Arabidopsis thaliana] gb\|AAF14230.1\|AF109182_1 magnesium/proton exchanger AtMHX [Arabidopsis thaliana] gb\|AAC62871.2\| putative Na+/Ca2+ antiporter	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	33%
Blast2go	magnesium proton exchanger	-	-	71%

Source	GOs	Term	Type	Identity
Blast2go	magnesium ion transport	GO:0015693	Biological Process	71%
Blast2go	zinc ion transport	GO:0006829	Biological Process	71%
Blast2go	iron ion transport	GO:0006826	Biological Process	71%
Blast2go	cation:cation antiporter activity	GO:0015491	Molecular Function	71%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	71%

Source	InterPros	Term	Type	Identity
Sma3	Kinesin, motor domain	IPR001752	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Sodium/calcium exchanger membrane region	IPR004837	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A901

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 83 aas, your sequence is shorter than subject: 74 - 242

Fln protein: MQMFGAPYVGFADGAYRSARNLSSAAWVIYDPHGELIDLQGVCLGCTTNNVAEYSVVIELPAEAINLGIRNWL*

Protein Length: 75

Fln nts: TTGATactATACTCGGGCAACGGGAgGCGTGTAGCCCGATTTTAATTCAGAAAAAGTCACCTACTCTGCAAGCAGGGGTTGCAATGTATTCTAGTAATGAATCTTGCTCTTCAGATGGAAAAAAGTGGCACATTTTATTGAGTGGCATTTTCAGATTCATTCCAGATAATTCAACAAGTTTGTTGGAGAATAAATGTGACAGCTACCTTTTGTTTCATTTTGAAACTAATGTTGGGATTGGCACCCGGTCATTTCTATATGTTTTGGGCCTTGCTTATTGTTTTATTGGATTGTCTGCCATAACTGCTTGCTTCTTTCGTTCAATGGAGAATGTTGTGAAACAGACAAGGGAGATTATTTATGTGGATCCTGTAACGGGTGCTAAAAGAGTCAGGAGAGAAAGGATATGGAACTATACATTGGCAGATATTAGTTTATTAGCTCTTGGGACAAGCTTTCCACAGATATCACTTTCTACGATTGACGCTTTTCAGAACCTTGGTCAACGATATGCTGGGGGTTTGGGTCCTGCAACACTCGTGGGTTCTGCTGCATTTGACCTCTACCCTATCCATGCCATTTGTGTAGTAGTTCCAAGAGCAGGCACAGTGAAAAAAATCTCAGATTTAGGAGTATGGTTGGTTGAACTTACATGGTCTTTTTGGGCATATGTTTGGCTTTACATAATAATAGAGGAAAATGTTGTGGGGAAACCCAAGGTTGCTTCCCGACATTCATGGATGACATCCTTAGTAAACTACTTGTTGTACACTGGTGTGCCTAATCCAGGGGGATTCAATCCAGGTGTGGACTCCGAATGTGGTGACTATCTGGGAGGCTGGATTGACTATAACACAGTTTGGCTTACTAATGTTACATGCATATGGACAAGACAGAGGGTGGCGCTGTGTTGCGATTCCCTTGCCAAGACAAGAGAGGCCAGCAGCTTGGGTGCCTGTTGATACTACATATGGTTGCAGCCAAGATGGAAATCATGAAAAGTATTCTGAGTCTGCTTTATCTGACTGCATGCCTTCAGATTCTGCTGTTGATATCTTCTCAATACACAATGATTCCATATCTGAGGAAGTACCTGGGAGGGAAAATGTTGAATCTTCCTTTCTACCCTGGGATCCTGATGACATCATTGGAACTGAAAGAAAGGATATGTTCTTGGTTTGGCGTCAACAGTTCCACAATGTTCTCACTCTGGAAGATCGTAAAACAAGAACGAATAGTAAGGCTCCATTGCGAGTAATGAGAATAGTTTGGCAATTGATAATTCTACCATGGAGGTTTTTATTTGCCTTCGTCCCCCCATGTGATATTGCACATGGATGGGTAGCTTTCAGTGTTTCGCTAATTTTTATAACTGGCATTTCCTATGTTCTTACAAAGCTTACAGATTTGATCAGCTGTGTGACAGGAGTGAATCCATATATCATAGCTTTCACAGCCCTTGCAAGCGGAACCTCATGGCCTGATCTAGTTGCAAGTAAAATAGCGGCAGAACGCCAAGAGACAGCAGATTCTGCTATTGCAAACATCAC_-_ATGCAGATGTTTGGTGCACCTTATGTTGGTTTTGCTGATGGTGCGTACCGCAGTGCCCGCAACTTGTCTTCTGCGGCATGGGTGATCTACGATCCTCATGGTGAGTTGATAGACCTCCAAGGTGTTTGTCTAGGTTGTACCACAAACAATGTCGCTGAATATAGTGTTGTTATTGAGTTACCGGCTGAGGCGATCAATCTCGGTATTCGTAATTGGTTGTGAACCTGGACTCTCAGCTTGTCGTCCACCAGATCAATGGGCGATTTTTTGTCAGAGACCATCGGATTTTAAGGTTGTATTTACGTGTTAAATTGTTAGAAAGGAATTTTGATTTTATCACATATCAGTATATTCCACGAAGATTAAACACGTTAACTGATGCATTAGCTAATCATGTATTAGACAAACATTTGCTTAACATGTAATCAAA

Fln Alignment:

Contig23863___FADGAYRSARNLSSAAWVIYDPHGELIDLQGVCLGCT-TNNVAEYSVVIELPAEAINLGI
D5A901_______________YFDGACRGNPGTAGIGFLLKDSNGEVVERFCSLLGYKLTNNVAEYKALIAGLQHALDHGI

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
286	25			75	0.996473
506	87		12		0.992554
1669	90		10		0.90298
1756	11			88	0.923564
1776		12		87	0.992554
1828		85		14	0.993533
1861	85		14		0.993533
1869	85		14		0.993533
1919	87		12		0.992554
1934		12		87	0.992554

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain