SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene23452

Length: 1643 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene23452 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Calcineurin B-like protein 01 n=1 Tax=Selaginella moellendorffii RepID=C4P7X6_SELML	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	73%
Blast2go	calcineurin b-like protein 4	-	-	84%

Source	InterPros	Term	Type	Identity
Sma3	Toll/interleukin-1 receptor homology (TIR) domain	IPR000157	-	-
Sma3	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	-
Sma3	Recoverin	IPR001125	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Protein phosphatase 2C-like	IPR001932	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	NB-ARC	IPR002182	-	-
Sma3	AAA+ ATPase domain	IPR003593	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3		IPR014045	-	-
Sma3		IPR015757	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKN8

Fln msg:

Fln protein: MGCVGSKTKTVKQSPANEDPVVLSAETAFTVSEVEALHELFKKLSSAVFNDGRIHKEEFQLALFRNSQRENLFVDRVFDLFDIKRNGVIEFGEFVRSLSIFHPNASKEGKIAFAFKLYDLRQTGYIEREEVKQMVIALLNESDMNLSDEVVETILDKTFAEADTKGDGRIDPEEWRDFVRCNPSLMKNMTLPYLKDITTAFPSFVFKSEVDEAAS*

Protein Length: 217

Fln nts: ATGGGGAAACGAAAGCGGGGCTGTAGGAGGAGCTGTGGCACCTGCTATCGGGACCGGGGAAAGGGAGAAAAGAGGAGTTCCAGTCGCAGGAGTCTTGAATTTATATATCGCATATATACGCATAGTGATTGAGAGATTCTGAACTCGGAGGAAAGTTTGTGGCAGTTGCACTGTTGGATCATGCAACACATTTTGTTATGGGCTGTGTTGGATCCAAGACTAAGACGGTTAAGCAATCACCTGCAAACGAAGACCCTGTTGTTCTTTCTGCAGAAACAGCATTGATTGtGAGATTCTGAACTCGGAGGAAAGTTTGTGGCAGTTGCACTGTTGGATTATGCAACACATTTTGTT_-_ATGGGCTGTGTTGGATCCAAGACTAAGACGGTTAAGCAATCACCTGCAAACGAAGACCCTGTTGTTCTTTCTGCAGAAACAGCATTCACTGTGAGTGAAGTTGAGGCCTTACACGAGCTTTTCAAGAAATTAAGTAGTGCTGTCTTTAACGATGGGCGGATTCACAAGGAAGAATTTCAGCTAGCACTCTTTAGAAACTCTCAAAGGGAGAACCTCTTTGTAGACAGGGTTTTTGATCTCTTTGACATCAAGCGTAATGGAGTTATAGAGTTTGGAGAATTTGTGCGATCATTGAGTATTTTCCATCCAAATGCATCTAAAGAAGGCAAAATTGCATTTGCCTTTAAACTGTATGACTTGAGACAAACTGGGTACATTGAACGTGAAGAGGTGAAGCAGATGGTGATAGCTCTTCTAAATGAATCTGACATGAACCTTTCAGATGAAGTTGTTGAAACGATTTTAGATAAGACATTTGCTGAAGCAGATACCAAGGGCGATGGAAGAATTGATCCAGAGGAATGGCGAGATTTTGTGAGGTGCAATCCCTCTCTGATGAAGAATATGACTCTACCCTACCTGAAAGATATCACTACTGCTTTTCCAAGCTTTGTCTTCAAATCCGAAGTGGATGAGGCAGCAAGTTGA___TTAAACATTTTGGGAATCACCTTCCGGTGTGCTAGTCAGCTTCAAAGCAATTCGGATTGATATTGTTATGGAATCTGGTGAAGAACTTCAACAAATCCTTTTTAGTACAAAATTCCTTATAATATATAACTATTCTCAAGCAGGATCCCAGTACCCATCGTTACATACGAATGTGTATGTGCTGATTGAATACACAATCTTAGTGGTGATATTTATTGCACAAAATGACCAAAGCTTTTTGATTTGGCCAAAGTGATCTAGGAATAACCCTCTAAATAATGAGGGTTTTGTTAACTGCATGGTTTTGGGATTCGTGAGGTAAACTGTTGAATTATATTATATCTGAAACTTGATGTTCTGCAAAGAAAACTGACGTAGTCTGAAGGGAAAGGTCAGTTTCACATATGCAAAACAGCAATATATGAAAAGAATTCCTACTGGTTTTTGAGGGAACTATTTTAACTTTTATCAGAGTCAGTTTATGAAGGGGTTACATGAGAGAACTCCAGCCATGAATTACTGAATAGAAAATGTGCAAACAAAAATGAGGAAGTATTCATTTCCTATGCACAAATGGTCACAATTCCTTAAAATTGATTTCGTGTGTATAAACAAAGCTGAAGCCATTTAACGAATCAGTT

Fln Alignment:

Contig23452___MGCVGSKTKTVKQSPANEDPVVLSAETAFTVSEVEALHELFKKLSSAVFNDGRIHKEEFQLALFRNSQRENLFVDRVFDLFDIKRNGVIEFGEFVRSLSIFHPNASKEGKIAFAFKLYDLRQTGYIEREEVKQMVIALLNESDMNLSDEVVETILDKTFAEADTKGDGRIDPEEWRDFVRCNPSLMKNMTLPYLKDITTAFPSFVFKSEVDEAAS
B8LKN8_______________MGCFNSKF--TKHSPGYEDPVKLAAETAFSVSEVEALYELFKKISSSVIDDGLINKEEFQLALFKNSKKENLFADRVFDLFDVKRKGVIDFGDFVRSLSVFHPNAPQEDKIDFAFKLYDLQCTGYIEREEVKQMLIALLCESEMKLADEVIETILDKTFQEADVDQDGRIDRDEWQKFVSHNPSLMKIMTLPYLKDITTTFPSFVFNSEVDDIAT

SNPs (tot: 38)

Position	A %	C %	G %	T %	Probability
212			25	75	0.996473
213	25	75			0.996473
214			25	75	0.996473
216			25	75	0.996473
217	25			75	0.996473
220			25	75	0.996473
221	75	25			0.996473
222		25		75	0.996473
224	25			75	0.996473
225			25	75	0.996473
227		75		25	0.996473
228		25		75	0.996473
229	25		75		0.996473
230		25		75	0.996473
231			25	75	0.996473
232	25			75	0.996473
233		25		75	0.996473
475		12	87		0.992554
540	50			50	1.0
605		16		83	0.994512
606		16	83		0.994512
607	83	16			0.994512
608	16	83			0.994512
640		20		80	0.995493
693		85		14	0.993533
819	10			90	0.90298
845		11		88	0.923564
873		10		90	0.90298
907	88			11	0.923564
1282	85		14		0.993533
1334		25		75	0.996473
1469	28		71		1.0
1522		83		16	0.994512
1529		83		16	0.994512
1534	83		16		0.994512
1554	83	16			0.994512
1555	16		83		0.994512
1576	16			83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain