SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene22388

Length: 2043 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene22388
GTAGCACATGTCATAGAACCATGCGTTTTCCTTGAGCTGCGTTGAAAATTGGGGATTAATGAAATCCTGTTGAAAATGGCCAGAGGTGGAGAAGTGTCTACTAAAGCCATGGCTTCCAAATGGATAGTGTTACTTTGTACTGCAAGCTTCTGCGTGGGAATGTTTTTTACCAATAGAATGTGGAGCATTCCAGAAGCTAAAGATGTAATTAAATTTAGCAGAGAACTGGGAGATCAGCTCAGGCTTGTTTCTGAAGGATGTGATCCCAAACCAAAACCTGTAGATTCCAGGCAATATTTGGGAGAATTTTCTAAAACAGATCAAAGTATAGAAAACATTGGTAAAAAAATCTCAGAGTTGGAAAGAGAGCTAGCAGCAGCAAGGTCTGCTCAAGTATCCCTACTGAATGGTGCTCCAATTTCAGAAGAGCCTGTTATTACGAATACAGCAGAACGGCCAAAGGCTTTTATGATTGTTGGAATCAATACTGCTTTCAGTAGCAGAAAGCGCCGTGATTCAGTCCGTTCAACATGGATGCCACGGGGTGAAAGGCGAAGGAAGATGGAAGAGAAAGGCATCATTGTCCGTTTTGTCATAGGTCACAGTGCCACAGCAGGTGGAATTCTGGACAGAGCAATTGAAGCTGAAGACATACAACACCACGACTTCATGAGACTGGATCATGTTGAAGGGTATCTTGAGCTGTCTGCTAAGACAAAAATATACTTTGCTACGGCTTATGCAAAATGGGATGCAGATTTTTATATAAAAGTTGACGATGATGTCCATGTAAATCTAGGGACCCTTGCAAAAACTCTTGCAAGGCATCGATCAAAGCCTCGAGTGTACACAGGATGTATGAAGTCTGGTCCTGTCCTGGCGAATAAGGGGGTTAGGTACCATGAACCGGAATACTGGAAATTTGGTGAAGTGGGAAATAAATACTTCAGACATGCAACTGGTCAACTTTATGCCATCTCAAAGGATTTGGCATACTATGTATCCCAAAATCTAGATATATTGCATAGATATGCAAATGAGGATGTTTCTATCGGATCCTGGTTTATAGGGTTGGATGTGAAACACATTGACGATCGGAAAATGTGTTGTGGAACTCCTCCAGATTGTGAATGGAAGGCACAGTCAGGGAATGTATGCGTTGCATCTTTTGATTGGAGTTGCAGTGGCATTTGCAAGTCTGCAGAGAGAATTAAGGAAGTGCATCGTCGCTGCAAGGAAAGCGACAATGTTGTATGGAATGCATCCTTTGCAGGGCTTAATCTCAACTACCAAAGGAGTTGACATGATCATTAGACATGGCCATTAGCCCGTTAAGCATATGTATCCATGACTGTAAGGTTGGAAAGGTGAAGCAAGATTCACGCAAGTTGAGACTACTACTTATCAAATTGCTTTCCTGTCCAGTATAAATAGTGAAATTTGTTTGATAGCTATATATGAGTGCCATTTGTATGCAACTCAAGTTTAGATTTGGGTAAGGAGTATGTTTCTCACAAGGCAACGATTTTTCCTTCTCTTGCCATGGAGATGACTATAGACAAATAACCTGGGCTTCTACTTCAAAAGGAAGTTATGGAACTCATGTGGATCATTATTTTAGAGGGCATCTACTAGTCTGTGAGCAATTGCCTTAATTGCTCGGAATTAACACACTGACAGTCACGGACAGTTCTAATGATTGATCTTGCATTCCTGTGGGTATTTAATCCCACATCAACAAGTATGATTTCTACACTGGTTAAAGCATCCAACTCCTGGAGCCAAAGCTGAAGATTCATTTAGGATATATTGTATAAGAGCTGGGGTTGATATTGTCTGGCAGTCACTTTGTGCCATACATCATTGCCTCATTCCTATTAAATGTAATACCAATCATAAAAGGATATTATCATGTAGTGGATGGCTGTATTATCAGCTATGAAAATTAGGTGGGTAAATGATCATCCTTATTTTAACAAGGACTTGGAAAAATCACAATACAAGTATCATTGATAATGCAATCTATTATTCTTTGTTCAAAGG

Ace file of the UniGene sp_v2.0_unigene22388 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative beta-1,3-galactosyltransferase 2 [Arabidopsis thaliana] gb\|AAK63859.1\|AF389287_1 At1g05170/YUP8H12_22 [Arabidopsis thaliana] gb\|AAB71461.1\| Similar to Sequence 10 from patent 5477002 (gb\|1253956) [Arabidopsis thaliana] gb\|AAM47315.1\| At1g05170/YU	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	75%
Blast2go	probable beta- -galactosyltransferase 2-like	-	-	81%

Source	GOs	Term	Type	e value	Identity
Blast2go	transferase activity	GO:0016740	Molecular Function	0.0	81%

Source	InterPros	Term	Type	Identity
Sma3	GPCR, rhodopsin-like, 7TM	IPR000276	-	-
Sma3	RNA recognition motif domain	IPR000504	-	-
Sma3	Glycosyl transferase, family 31	IPR002659	-	-
Sma3	2Fe-2S ferredoxin, iron-sulphur binding site	IPR006058	-	-
Sma3	Nucleotide-binding, alpha-beta plait	IPR012677	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AE09

Fln msg: your sequence is longer than subject: 409 - 335

Fln protein: MARGGEVSTKAMASKWIVLLCTASFCVGMFFTNRMWSIPEAKDVIKFSRELGDQLRLVSEGCDPKPKPVDSRQYLGEFSKTDQSIENIGKKISELERELAAARSAQVSLLNGAPISEEPVITNTAERPKAFMIVGINTAFSSRKRRDSVRSTWMPRGERRRKMEEKGIIVRFVIGHSATAGGILDRAIEAEDIQHHDFMRLDHVEGYLELSAKTKIYFATAYAKWDADFYIKVDDDVHVNLGTLAKTLARHRSKPRVYTGCMKSGPVLANKGVRYHEPEYWKFGEVGNKYFRHATGQLYAISKDLAYYVSQNLDILHRYANEDVSIGSWFIGLDVKHIDDRKMCCGTPPDCEWKAQSGNVCVASFDWSCSGICKSAERIKEVHRRCKESDNVVWNASFAGLNLNYQRS*

Protein Length: 410

Fln nts: GTAGCACATGTCATAGAACCATGCGTTTTCCTTGAGCTGCGTTGAAAATTGGGGATTAATGAAATCCTGTTGAAA_-_ATGGCCAGAGGTGGAGAAGTGTCTACTAAAGCCATGGCTTCCAAATGGATAGTGTTACTTTGTACTGCAAGCTTCTGCGTGGGAATGTTTTTTACCAATAGAATGTGGAGCATTCCAGAAGCTAAAGATGTAATTAAATTTAGCAGAGAACTGGGAGATCAGCTCAGGCTTGTTTCTGAAGGATGTGATCCCAAACCAAAACCTGTAGATTCCAGGCAATATTTGGGAGAATTTTCTAAAACAGATCAAAGTATAGAAAACATTGGTAAAAAAATCTCAGAGTTGGAAAGAGAGCTAGCAGCAGCAAGGTCTGCTCAAGTATCCCTACTGAATGGTGCTCCAATTTCAGAAGAGCCTGTTATTACGAATACAGCAGAACGGCCAAAGGCTTTTATGATTGTTGGAATCAATACTGCTTTCAGTAGCAGAAAGCGCCGTGATTCAGTCCGTTCAACATGGATGCCACGGGGTGAAAGGCGAAGGAAGATGGAAGAGAAAGGCATCATTGTCCGTTTTGTCATAGGTCACAGTGCCACAGCAGGTGGAATTCTGGACAGAGCAATTGAAGCTGAAGACATACAACACCACGACTTCATGAGACTGGATCATGTTGAAGGGTATCTTGAGCTGTCTGCTAAGACAAAAATATACTTTGCTACGGCTTATGCAAAATGGGATGCAGATTTTTATATAAAAGTTGACGATGATGTCCATGTAAATCTAGGGACCCTTGCAAAAACTCTTGCAAGGCATCGATCAAAGCCTCGAGTGTACACAGGATGTATGAAGTCTGGTCCTGTCCTGGCGAATAAGGGGGTTAGGTACCATGAACCGGAATACTGGAAATTTGGTGAAGTGGGAAATAAATACTTCAGACATGCAACTGGTCAACTTTATGCCATCTCAAAGGATTTGGCATACTATGTATCCCAAAATCTAGATATATTGCATAGATATGCAAATGAGGATGTTTCTATCGGATCCTGGTTTATAGGGTTGGATGTGAAACACATTGACGATCGGAAAATGTGTTGTGGAACTCCTCCAGATTGTGAATGGAAGGCACAGTCAGGGAATGTATGCGTTGCATCTTTTGATTGGAGTTGCAGTGGCATTTGCAAGTCTGCAGAGAGAATTAAGGAAGTGCATCGTCGCTGCAAGGAAAGCGACAATGTTGTATGGAATGCATCCTTTGCAGGGCTTAATCTCAACTACCAAAGGAGTTGA___CATGATCATTAGACATGGCCATTAGCCCGTTAAGCATATGTATCCATGACTGTAAGGTTGGAAAGGTGAAGCAAGATTCACGCAAGTTGAGACTACTACTTATCAAATTGCTTTCCTGTCCAGTATAAATAGTGAAATTTGTTTGATAGCTATATATGAGTGCCATTTGTATGCAACTCAAGTTTAGATTTGGGTAAGGAGTATGTTTCTCACAAGGCAACGATTTTTCCTTCTCTTGCCATGGAGATGACTATAGACAAATAACCTGGGCTTCTACTTCAAAAGGAAGTTATGGAACTCATGTGGATCATTATTTTAGAGGGCATCTACTAGTCTGTGAGCAATTGCCTTAATTGCTCGGAATTAACACACTGACAGTCACGGACAGTTCTAATGATTGATCTTGCATTCCTGTGGGTATTTAATCCCACATCAACAAGTATGATTTCTACACTGGTTAAAGCATCCAACTCCTGGAGCCAAAGCTGAAGATTCATTTAGGATATATTGTATAAGAGCTGGGGTTGATATTGTCTGGCAGTCACTTTGTGCCATACATCATTGCCTCATTCCTATTAAATGTAATACCAATCATAAAAGGATATTATCATGTAGTGGATGGCTGTATTATCAGCTATGAAAATTAGGTGGGTAAATGATCATCCTTATTTTAACAAGGACTTGGAAAAATCACAATACAAGTATCATTGATAATGCAATCTATTATTCTTTGTTCAAAGG

Fln Alignment:

Contig22388___LNGAPISEEPVITNTAERPKAFMIVGINTAFSSRKRRDSVRSTWMPXXXXXXXXXXXG-IIVRFVIGHSATAGGILDRAIEAEDIQHHDFMRLDHVEGYLELSAKTKIYFATAYAKWDADFYIKVDDDVHVNLGTLAKTLARHRSKPRVYTGCMKSGPVLANKGVRYHEPEYWKFGEVGNKYFRHATGQLYAISKDLAYYVSQNLDILHRYANEDVSIGSWFIGLDVKHIDDRKMCCGTPPDCEWKAQSGNVCVASFDWSCSGICKSAERIKEVHRRCKESDNVVWN
D5AE09_______________LNGSPVLEKPDGRGSEVRQKAFVVIGINTAFSSRKRRDSVRETWMPQGPKLKQLEEKKGIVIRFVIGHSVTPGGILDRAIDAEDAQHNDFLRLEHVEGYHELSAKTKTYFSTAVAKWDADFYVKVDDDVHVNLGMLATTLSRYRSKPRIYIGCMKSGPVLAQKGVKYHEPEYWKFGEEGNRYFRHATGQIYAISRDLATYISINSPILHRYANEDVSLGAWFIGLDVEHIDERSMCCGTPPDCEWKAQAGNVCVASFDWTCSGICKSVERLKEVHRTCSEGDGAVWS

SNPs (tot: 65)

Position	A %	C %	G %	T %	Probability
341		20		80	0.995493
359		20		80	0.995493
434	25			75	0.996473
447		75		25	0.996473
548		66		33	0.997452
666	12			87	0.992554
736	14		85		0.993533
1000	83			16	0.994512
1152		83		16	0.994512
1153		83	16		0.994512
1154		83		16	0.994512
1202	12	87			0.992554
1203		12	87		0.992554
1205			87	12	0.992554
1206			87	12	0.992554
1280		12		87	0.992554
1282	87			12	0.992554
1283	87			12	0.992554
1288		71		28	1.0
1378	90		10		0.90298
1382	10			90	0.90298
1383		10	90		0.90298
1384	10		90		0.90298
1418	87			12	0.992554
1421	87		12		0.992554
1423			12	87	0.992554
1424	12	87			0.992554
1427		87	12		0.992554
1429		12		87	0.992554
1430	12		87		0.992554
1431	12	87			0.992554
1432			12	87	0.992554
1435		14	85		0.993533
1436	14		85		0.993533
1452	83			16	0.994512
1456	83			16	0.994512
1458		83		16	0.994512
1459			83	16	0.994512
1462	16	83			0.994512
1463	83		16		0.994512
1465			16	83	0.994512
1467	83		16		0.994512
1468	20			80	0.995493
1470	40	60			1.0
1471		80	20		0.995493
1472	40			60	1.0
1473	20			80	0.995493
1474	20			80	0.995493
1475		75	25		0.996473
1476			25	75	0.996473
1478	25			75	0.996473
1484		75		25	0.996473
1485			25	75	0.996473
1496	25			75	0.996473
1498		75		25	0.996473
1500		66		33	0.997452
1501		66		33	0.997452
1696	20			80	0.995493
1697		20		80	0.995493
1699	20			80	0.995493
1700			20	80	0.995493
1788	16	83			0.994512
1789	16	83			0.994512
1791			14	85	0.993533
1792		83		16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain