SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene22096

Length: 2720 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene22096
CCTAGGTTTCTCGACCCTTTTAGATTGCTGTCTGGAGTTAGTAATTCACGCGTTTAGCATCCCGGCTAAACTTGCTGTAGGTCTGTCCATGATATTTGCATGCGGAGAATTTTCTTGAAAGGCTTTGCAGAGGGCTAGAATTTGCAGCTGCTAGGGTTAAAGATTGATTGTGGATGTTGCTGCTCGGGCATGCTTTACATGATCTTTACTTGCTGTAGGTCTCTCCATGATATTTGCGTGCGGCGAATTTCCTTGAAAGGCTTTGCAGAGGGCTAGAATTTGCAGCCGCTAGGATTAAAGATTGATTGTGTCTGTTGTTGTAGCTGCTCGGACTTGCTTTACATGATTTTGCATGATGCCGGATACAATGCCAGAGGATTCGGAAACTGAGTTCGGTAACACGGATTCCCAGCGTCCCGGGATTCGAACTTGTGACCAGAGCTCGACGAGTCGGGAGATTCGAAACGATCCGCAGAGCTGCCATGAATCTGCGTTTGTTGGCCTGAGTTCGGTGATGGAGAAGAACGAAGCGTTTTCTTGTGGGAACGAGGAGGATAAAGGGAAAACGCGGTTTTCTGGAGATGAATGCGCGAGGCCTGAAATTGGCTGTTCCAGTGCCAGAGAATACGATTACATAGGCTTTAAATCTGAAGCTGCTACTATCGGGAATAAGTCGAAAGAGGAGAATTGTATCAAGCTGGGTCCTAAGACGAAAGAAACCGAGCTAAGATTAGGTCTTCCAGAGGACGACGAGCTGGATGGTGGAGCCGACGTCGCGGGTTCGAGACGGACGCCGGATGCGAAAAGTCTGACTATTGAGGTAACGGAGCCGCCTCGGCTTGATCTTTCTAGTGTCCACAGAGGGAAAGCTGACGAGTACGGTCCCCAGCGGGATTCGATCCTGGAACGGGAGAGCCGCTACGTTAATAGCCAAGAGATTTCCGGATATAATAGCTTTGTTTTCGCGAAGAAAGGTTCTAATCTGGTGGACGAAGCTGGCCAGGCAAAGCTTAATGATGTTTCTCCTTCTTCTCTGAAGGATCTCCGTTTACCTTTGCACCCGGGAGCATATGATTCTCGGTTCATCCAAAGCGGGGAGGCCCAGAAATGCTGGATGAACTCGTTCAACAGCCGTGAGTTTTCCCAGGTTGTACAAGATTACAATTACAGGGCTCTTCACGCGGCATCGAAGGGTATTGCTTCAGGAGCAAAGCGAGGTTTCTCTGATGCACTGGGAGTGAATTTGATGCGTGATTCTGGGTTTGGATTACCAGAGTCGGACTCAAAGTTGTTGAATCAGTCACAGCCAAGGAATTTCATATTTCCTTGGGCTGTTCCGCAGCAGCATGGCCCTGCAAACGCTTGGCTCACTTGGGAGCAGAAAAGCAAGCAGAATTTGCAACACTATTCCAAGGCTTTAGCGCCCAGCGGGATACCAGATGATAAAACGAACAATCGATTTGGGGAGGGTGCTATATCCACCCAAAATACTCAATCTGTAGTCAAAGCTTCTCAGGAGAGCCAGAGACCGCCGGCACCTGTTGCTGCCTCTGAAAAGATACAAACCCCAGCTGAGACTGATCGTGCTCCGAACCAAAGTGTAACCCCTTCCAGAGCACCCCCGGTGGTAGGATGGCCTCCTATCCGGAGCTTCAGAAAGAATTTGGCTGCGCAACCCAAAGTAGCCGCGGCCCCCTCTTGCAATCCTCCTCCACCTCCAGCAGCAGATCCAGGGGAAAAGAAAATAAACACCATGTTCGTCAAAGTAAATGTGGATGGCGTGCCTATAGGCCGCAAAATTGACTTGAAGGCCTATGACAGTTATGAAAAGCTCACTGCTGCTCTGGATGAAATGTTCCGAGTCGCCATAAATGCTCAAACATCGGATGCAAATCCTTTACCTGAAAATAATAATAATCAGGCGAGTCTCTTGAATGGAAGGGACTATGTTTTTGTGTACGAGGACAATGAAGGAGATCGCATGCTTGTTGGAGATGTGCCTTGGGACATGTTTGTGAAGTCTGTGAAGAGATTGCGAGTAATGAAGAGTTCAGATGCTCGTCGTCTAGCAGCAAACAGGACCCAGTGATCCCAACTGCCTAATGCTGCTGGAATCAAGAGGGGAACCCTAGCAAGTTTATCTCACAGTAACCGTAAGAAAGAAGATGAACCCAATGTTCCTTCTCTGAGAGAGTTCATGTACTATTTTATCCAGCTGCATTGTGGAGTGAAACTGGAGAGGAGTTTTTGTAGCATATATTAGACAAAGTTAAGGTCAATAGAATAGTGTTGAAAGATTCAGTTCATTGAGAGATCAGTAATACGGGTTGAGCAAAATAAAACAGTAAATGGTGTAAAGATGGCTGGTTTGGTCCAGTGAGACCAATTTCCTTTATTTTTTTCTTGGTGTTGATTGTACTACCTTGTTCGATTATTAAATGTTTGTTGTTCAAGATACCATGCTTTGCTCAGCGACTGTACGCCATTGTAGAGGTATGTGATTAGCTAACAGCGCAAACCATGGAGTCACTCTTCTGTTCGTCTCGTTATATTGTATATTAATTGCCACATGTATAATATTAAATGCGGTCTTCTTTTATAGAATCCCACATGTATACCAATAGGAATTTTGTAATTTTCTGTCTGTTGTACAGGCATCATACTCCTATCCATTCTTGGGTTATCAACGGACTCATTTATAGATATAATCATTTGAATTA

Ace file of the UniGene sp_v2.0_unigene22096 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Auxin-responsive protein IAA7 n=1 Tax=Picea abies RepID=B2KZF8_PICAB	-	-	74%
FL-Next	tr=Auxin-responsive protein IAA7; Picea abies (Norway spruce) (Picea excelsa).	-	-	78%
Blast2go	phytochrome-associated protein 1	-	-	59%

Source	ECs	Term	Type	e value	Identity
Blast2go	Phosphatidate phosphatase.	EC:3.1.3.4	-	0.0	59%

Source	KEGGs	Term	Identity
Blast2go	Glycerolipid metabolism	00561	59%
Blast2go	Glycerophospholipid metabolism	00564	59%
Blast2go	Ether lipid metabolism	00565	59%
Blast2go	Sphingolipid metabolism	00600	59%
Blast2go	Metabolic pathways	01100	59%

Source	GOs	Term	Type	Identity
Blast2go	cellular response to phosphate starvation	GO:0016036	Biological Process	59%
Blast2go	response to salt stress	GO:0009651	Biological Process	59%
Blast2go	response to jasmonic acid stimulus	GO:0009753	Biological Process	59%
Blast2go	removal of superoxide radicals	GO:0019430	Biological Process	59%
Blast2go	regulation of anthocyanin biosynthetic process	GO:0031540	Biological Process	59%
Blast2go	sucrose mediated signaling	GO:0009745	Biological Process	59%
Blast2go	response to ethylene stimulus	GO:0009723	Biological Process	59%
Blast2go	response to auxin stimulus	GO:0009733	Biological Process	59%
Blast2go	protein binding	GO:0005515	Molecular Function	59%
Blast2go	phosphatidate phosphatase activity	GO:0008195	Molecular Function	59%
Blast2go	plant-type cell wall	GO:0009505	Cellular Component	59%
Blast2go	plasma membrane	GO:0005886	Cellular Component	59%
Blast2go	nucleus	GO:0005634	Cellular Component	59%

Source	InterPros	Term	Type	e value	Identity
Sma3	AUX/IAA protein	IPR003311	-	0.0	-
Sma3	Aux/IAA-ARF-dimerisation	IPR011525	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B2KZF8

Fln msg: your sequence is longer than subject: 579 - 256

Fln protein: MMPDTMPEDSETEFGNTDSQRPGIRTCDQSSTSREIRNDPQSCHESAFVGLSSVMEKNEAFSCGNEEDKGKTRFSGDECARPEIGCSSAREYDYIGFKSEAATIGNKSKEENCIKLGPKTKETELRLGLPEDDELDGGADVAGSRRTPDAKSLTIEVTEPPRLDLSSVHRGKADEYGPQRDSILERESRYVNSQEISGYNSFVFAKKGSNLVDEAGQAKLNDVSPSSLKDLRLPLHPGAYDSRFIQSGEAQKCWMNSFNSREFSQVVQDYNYRALHAASKGIASGAKRGFSDALGVNLMRDSGFGLPESDSKLLNQSQPRNFIFPWAVPQQHGPANAWLTWEQKSKQNLQHYSKALAPSGIPDDKTNNRFGEGAISTQNTQSVVKASQESQRPPAPVAASEKIQTPAETDRAPNQSVTPSRAPPVVGWPPIRSFRKNLAAQPKVAAAPSCNPPPPPAADPGEKKINTMFVKVNVDGVPIGRKIDLKAYDSYEKLTAALDEMFRVAINAQTSDANPLPENNNNQASLLNGRDYVFVYEDNEGDRMLVGDVPWDMFVKSVKRLRVMKSSDARRLAANRTQ*

Protein Length: 580

Fln nts: CCTAGGTTTCTCGACCCTTTTAGATTGCTGTCTGGAGTTAGTAATTCACGCGTTTAGCATCCCGGCTAAACTTGCTGTAGGTCTGTCCATGATATTTGCATGCGGAGAATTTTCTTGAAAGGCTTTGCAGAGGGCTAGAATTTGCAGCTGCTAGGGTTAAAGATTGATTGTGGATGTTGCTGCTCGGGCATGCTTTACATGATCTTTACTTGCTGTAGGTCTCTCCATGATATTTGCGTGCGGCGAATTTCCTTGAAAGGCTTTGCAGAGGGCTAGAATTTGCAGCCGCTAGGATTAAAGATTGATTGTGTCTGTTGTTGTAGCTGCTCGGACTTGCTTTACATGATTTTGC_-_ATGATGCCGGATACAATGCCAGAGGATTCGGAAACTGAGTTCGGTAACACGGATTCCCAGCGTCCCGGGATTCGAACTTGTGACCAGAGCTCGACGAGTCGGGAGATTCGAAACGATCCGCAGAGCTGCCATGAATCTGCGTTTGTTGGCCTGAGTTCGGTGATGGAGAAGAACGAAGCGTTTTCTTGTGGGAACGAGGAGGATAAAGGGAAAACGCGGTTTTCTGGAGATGAATGCGCGAGGCCTGAAATTGGCTGTTCCAGTGCCAGAGAATACGATTACATAGGCTTTAAATCTGAAGCTGCTACTATCGGGAATAAGTCGAAAGAGGAGAATTGTATCAAGCTGGGTCCTAAGACGAAAGAAACCGAGCTAAGATTAGGTCTTCCAGAGGACGACGAGCTGGATGGTGGAGCCGACGTCGCGGGTTCGAGACGGACGCCGGATGCGAAAAGTCTGACTATTGAGGTAACGGAGCCGCCTCGGCTTGATCTTTCTAGTGTCCACAGAGGGAAAGCTGACGAGTACGGTCCCCAGCGGGATTCGATCCTGGAACGGGAGAGCCGCTACGTTAATAGCCAAGAGATTTCCGGATATAATAGCTTTGTTTTCGCGAAGAAAGGTTCTAATCTGGTGGACGAAGCTGGCCAGGCAAAGCTTAATGATGTTTCTCCTTCTTCTCTGAAGGATCTCCGTTTACCTTTGCACCCGGGAGCATATGATTCTCGGTTCATCCAAAGCGGGGAGGCCCAGAAATGCTGGATGAACTCGTTCAACAGCCGTGAGTTTTCCCAGGTTGTACAAGATTACAATTACAGGGCTCTTCACGCGGCATCGAAGGGTATTGCTTCAGGAGCAAAGCGAGGTTTCTCTGATGCACTGGGAGTGAATTTGATGCGTGATTCTGGGTTTGGATTACCAGAGTCGGACTCAAAGTTGTTGAATCAGTCACAGCCAAGGAATTTCATATTTCCTTGGGCTGTTCCGCAGCAGCATGGCCCTGCAAACGCTTGGCTCACTTGGGAGCAGAAAAGCAAGCAGAATTTGCAACACTATTCCAAGGCTTTAGCGCCCAGCGGGATACCAGATGATAAAACGAACAATCGATTTGGGGAGGGTGCTATATCCACCCAAAATACTCAATCTGTAGTCAAAGCTTCTCAGGAGAGCCAGAGACCGCCGGCACCTGTTGCTGCCTCTGAAAAGATACAAACCCCAGCTGAGACTGATCGTGCTCCGAACCAAAGTGTAACCCCTTCCAGAGCACCCCCGGTGGTAGGATGGCCTCCTATCCGGAGCTTCAGAAAGAATTTGGCTGCGCAACCCAAAGTAGCCGCGGCCCCCTCTTGCAATCCTCCTCCACCTCCAGCAGCAGATCCAGGGGAAAAGAAAATAAACACCATGTTCGTCAAAGTAAATGTGGATGGCGTGCCTATAGGCCGCAAAATTGACTTGAAGGCCTATGACAGTTATGAAAAGCTCACTGCTGCTCTGGATGAAATGTTCCGAGTCGCCATAAATGCTCAAACATCGGATGCAAATCCTTTACCTGAAAATAATAATAATCAGGCGAGTCTCTTGAATGGAAGGGACTATGTTTTTGTGTACGAGGACAATGAAGGAGATCGCATGCTTGTTGGAGATGTGCCTTGGGACATGTTTGTGAAGTCTGTGAAGAGATTGCGAGTAATGAAGAGTTCAGATGCTCGTCGTCTAGCAGCAAACAGGACCCAGTGA___TCCCAACTGCCTAATGCTGCTGGAATCAAGAGGGGAACCCTAGCAAGTTTATCTCACAGTAACCGTAAGAAAGAAGATGAACCCAATGTTCCTTCTCTGAGAGAGTTCATGTACTATTTTATCCAGCTGCATTGTGGAGTGAAACTGGAGAGGAGTTTTTGTAGCATATATTAGACAAAGTTAAGGTCAATAGAATAGTGTTGAAAGATTCAGTTCATTGAGAGATCAGTAATACGGGTTGAGCAAAATAAAACAGTAAATGGTGTAAAGATGGCTGGTTTGGTCCAGTGAGACCAATTTCCTTTATTTTTTTCTTGGTGTTGATTGTACTACCTTGTTCGATTATTAAATGTTTGTTGTTCAAGATACCATGCTTTGCTCAGCGACTGTACGCCATTGTAGAGGTATGTGATTAGCTAACAGCGCAAACCATGGAGTCACTCTTCTGTTCGTCTCGTTATATTGTATATTAATTGCCACATGTATAATATTAAATGCGGTCTTCTTTTATAGAATCCCACATGTATACCAATAGGAATTTTGTAATTTTCTGTCTGTTGTACAGGCATCATACTCCTATCCATTCTTGGGTTATCAACGGACTCATTTATAGATATAATCATTTGAATTA

Fln Alignment:

Contig22096___IFPWAVPQQHGPANAW-LTWEQKSKQNLQHYSKALAPSGIPDDKTNNRFGEGAISTQNTQSVVKASQESQRPPAPVAASEKIQTPAETDRAPNQSVTPSRAPPVVGWPPIRSFRKNLAAQPKVXXXXXXXXXXXXXXXPGEKKINTMFVKVNVDGVPIGRKIDLKAYDSYEKLTAALDEMFRVAINAQTSDANPLPE-NNNNQASLLNGRDYVFVYEDNEGDRMLVGDVPWDMFVKSVKRLRVMKSSDARRLAANRTQ
B2KZF8_______________MFPWAVPQQYGSSNTWQINWEQKNKQNFQQYPQAIGPSEIPDEKTNNRFGEAAKSTANTQSVVKDSHESQRLPSAVAASEKIQTPAETERAPNQTGTPSRAPPVVGWPPIRSFRKNLASQPKVAAAPSCNPPPPAAEPV-EKKINTMFVKVNVDGVPIGRKIDLKAYDSYEKLSVALDEMFRGSINALTSDASPLAENNNNNQASLLNGRDYVFVYEDIEGDRMLVGDVPXXXFVNTVXRLRIMXXSXAGRL-ANRTQ

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
2244		88	11		0.923564
2319		10		90	0.90298
2353		11	88		0.923564
2451		42	42		1.0
2479		40	60		1.0
2552		80	20		0.995493
2571	25	75			0.996473
2573	75			25	0.996473
2622			83	16	0.994512
2658	80	20			0.995493
2659		20	80		0.995493
2662	20		80		0.995493
2663			80	20	0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
803	814	ATT ATT ATT ATT	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain