SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene21795

Length: 1279 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene21795 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Calmodulin binding protein, putative n=1 Tax=Ricinus communis RepID=B9RQR6_RICCO	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	36%
Blast2go	calmodulin binding	-	-	68%

Source	InterPros	Term	Type	Identity
Sma3	IQ motif, EF-hand binding site	IPR000048	-	-
Sma3		IPR000097	-	-
Sma3	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain	IPR000194	-	-
Sma3	Reverse transcriptase	IPR000477	-	-
Sma3	Endonuclease/exonuclease/phosphatase	IPR005135	-	-
Sma3		IPR015706	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NV67

Fln msg: your sequence is shorter than subject: 411 - 499

Fln protein: NVHESEGRDEGSFHKRVNSNGETSEVISAAAEAYNAAVATIVRAPPKDFKVVREEWAAIRIQTAFRGFLARRALRALKGFVRLQALFRGRQVRKQAAVTLRCMQALVRVQARVRARRVRMSTEGLAVQKMIDEHRIRQNKLRESEEGWCDSQGTLEEVRAKLQMRQEGAIKRERAIAYALSHQQWRSNSKTNMNYAKIEPDKNSWGWSWLERWMAAKLWENRLMEQAQMDACEIQSLKSYDDAGENLRKSHEPGKVKVRRNNISVRVAAKPPSAVSSHPTGRSSSNPGSDIQSDESSTISRGSCTPKSGNTLQTSDESVGRPNYMNSTESAKAKIRSSSAPKIRPSYDDLQLQKKVSSYNAEVKSTAGSDSSLHACKLIPPMLRSDKSSTKSGEISSCSYGESRKPFYQC*

Protein Length: 412

Fln nts: AATGTCCATGAATCCGAGGGGAGAGATGAAGGAAGTTTCCATAAGAGGGTAAATTCAAATGGCGAGACATCTGAAGTTATATCTGCTGCTGCGGAGGCTTATAATGCTGCAGTGGCCACTATAGTGAGGGCTCCTCCTAAAGACTTCAAGGTTGTCAGAGAAGAATGGGCCGCCATTCGGATACAGACTGCTTTTCGAGGATTTTTGGCCAGGAGGGCACTTCGAGCGTTGAAAGGGTTTGTGAGATTACAAGCATTGTTTCGAGGTCGTCAGGTAAGGAAACAAGCAGCAGTTACCTTGAGGTGCATGCAAGCACTTGTTAGAGTTCAGGCCCGTGTAAGAGCTCGCCGTGTTCGGATGTCGACTGAAGGCTTAGCGGTGCAGAAAATGATTGATGAACATCGAATTCGCCAAAACAAACTGCGAGAATCTGAGGAGGGGTGGTGTGATAGTCAAGGAACCCTTGAAGAAGTTCGTGCAAAACTGCAAATGAGACAAGAGGGTGCAATCAAACGTGAGAGAGCAATTGCTTATGCTCTCTCCCATCAGCAATGGAGAAGCAATTCGAAAACAAACATGAATTATGCTAAAATTGAGCCAGACAAAAATAGCTGGGGTTGGAGCTGGTTAGAACGCTGGATGGCTGCAAAGCTTTGGGAGAATAGACTCATGGAACAAGCCCAAATGGATGCTTGTGAAATTCAGTCTCTCAAAAGCTATGATGATGCTGGAGAGAATCTGAGGAAATCTCATGAACCAGGCAAAGTTAAAGTTCGCAGAAACAATATTAGTGTTCGTGTTGCTGCAAAACCACCTTCAGCAGTTTCCAGTCATCCTACTGGTAGATCATCTTCAAACCCTGGCTCTGATATCCAATCTGATGAGAGCTCAACAATCTCAAGGGGATCATGCACTCCAAAATCTGGAAATACTCTGCAGACATCAGATGAGAGTGTGGGAAGGCCTAATTACATGAACTCTACAGAGTCTGCCAAGGCCAAAATACGCTCGTCCAGTGCTCCAAAGATAAGACCCTCATATGATGATCTTCAATTGCAGAAGAAAGTTTCTTCATATAATGCTGAAGTGAAGAGCACAGCCGGTTCAGATTCTTCTCTGCATGCTTGCAAATTGATTCCACCTATGTTGAGATCTGACAAATCATCTACCAAGAGTGGAGAGATTTCTTCCTGTTCTTATGGAGAGTCCCGAAAACCCTTTTATCAATGCTAA___GTTTCAGAGTTCTATCCTGTCACTAACTAAGTAGGACTGAAGGTTG

Fln Alignment:

Contig21795___EEWAAIRIQTAFRGFXXXXXXXXLKGFVRLQALFRGRQVRKQAAVTLRCMQALXXXXXXXXXXXXRMSTEGLAVQKMIDEHRIRQ------NKLRESEEGWCDSQGTLEEVRAKLQMRQEGAIKRERAIAYALSHQQWRSNSKT-NMNYAKIEPDKNSWGWSWLERWMAAKLWENRLMEQAQMDACEIQSLKSYDDAGENLRKSHEPGKVKVRRNNISV----RVAAKPPSAVSSHPTGRSSSNPGSDIQSD----ESSTISRGSCTPKSGNTLQTSDESVGR----PNYMNSTESAKAKIRSSSAPKIRPSYDDLQ----LQKKVS-----SYNAEVKSTAGS--DSSLHACKLIPPMLRSDKSSTKSGEI------SSCSYGESRKPF
A9NV67_______________EECAAIKIQTAFRGYLVRRNFHTLRGLMRLQALVQGQSVRRQATNTMRCMQALVRVHSQICSRRIRMFEENQALQHHLQQKYEKELENRTSNSEADHQQDWESSLLTKEEIEARLQSKIEAAIKRERALAYAFSHHLWKNPPKSVQTMLMEIDPDKPHWGWSWLERWMATRPWDNHRMTMKENSTRKLQTI------GEIGQKTSHIG---LKQHNAEVTNIGTIKSDPFTPLSKPSIPNKMPLTGTDIKSDVNVLRSERPRYSSRYGVAGTSSLRDDESLMSSPRIPNYMASTESAKAKVRSQSTPKQRPGTPDTEPTSYRRKRLSFPLSEASSGPYRSTKGSMFPHTSPSLKGISGTSKSKKSVSSLRDLSIESDNSSLNWGH-RKPF

SNPs (tot: 30)

Position	A %	C %	G %	T %	Probability
179	12		87		0.992554
196		87		12	0.992554
212	62		37		1.0
223	12		87		0.992554
281	14		85		0.993533
339	11		88		0.923564
340		11		88	0.923564
341	11	88			0.923564
342			22	77	0.999999
343			88	11	0.923564
411	11			88	0.923564
414			88	11	0.923564
415	88			11	0.923564
418	12	87			0.992554
420			87	12	0.992554
421			87	12	0.992554
458		20		80	0.995493
728		20		80	0.995493
883	10	90			0.90298
886		90	10		0.90298
887	90			10	0.90298
902	20	80			0.999996
948	11		88		0.923564
950	11	88			0.923564
952			88	11	0.923564
990	12		87		0.992554
993			87	12	0.992554
1058	83	16			0.994512
1076	75			25	0.996473
1081		25		75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain