SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene21516

Length: 1007 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene21516 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	ssDNA-binding transcriptional regulator [Arabidopsis thaliana] gb\|AAF43941.1\|AC012188_18 Contains similarity to a hypothetical protein from Arabidopsis thaliana gb\|AC002521.2. EST gb\|AI995686 comes from this gene gb\|AAG48815.1\|AF332452_1 putative DNA-bind	-	-	56%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	70%
Blast2go	ssdna-binding transcriptional regulator	-	-	79%

Source	GOs	Term	Type	Identity
Blast2go	negative regulation of telomere maintenance via telomerase	GO:0032211	Biological Process	79%
Blast2go	telomeric DNA binding	GO:0042162	Molecular Function	79%
Blast2go	plastid chromosome	GO:0009508	Cellular Component	79%
Blast2go	chloroplast	GO:0009507	Cellular Component	79%
Blast2go	nucleus	GO:0005634	Cellular Component	79%

Source	InterPros	Term	Type	e value	Identity
Sma3	ssDNA-binding transcriptional regulator	IPR009044	-	0.0	-
Sma3	Plant transcription factor	IPR013742	-	0.0	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NLD1

Fln msg: Overlapping hits, possible frame ERROR between 457 and 451, your sequence is shorter than subject: 204 - 257

Fln protein: VVTLAVTQPGFLRGQPSKIYVKYTLYKGKAALGIQPKAPEYTTLDSGGIRLSKEGCVFLEFAPAVGTRQYDWSKKQIFALSVLELGTLLSFDPKEPCEFFHDPFMGKSEAGKIQKILKVGMLQDAGGYFFNLRVSDRTLNVDESLFMPIxxxxSPLCRWRFDFILPYLMGWHAYMNSEKPYESGHFTSASPSMGKKPDLEWGR*

Protein Length: 205

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGCCGGGCTTTTTGCGTGGTCAACCCAGCAAAATATATGTTAAGTATACATTATACAAAGGTAAAGCAGCACTTGGGATTCAACCCAAAGCACCCGAGTATACTACACTGGATTCTGGAGGAATCAGGCTTTCAAAAGAAGGTTGTGTGTTCCTTGAGTTTGCTCCTGCTGTTGGTACTCGCCAGTATGATTGGAGTAAGAAGCAGATATTTGCTTTATCTGTTCTTGAACTTGGAACCCTGTTAAGCTTTGATCCAAAAGAGCCATGTGAATTCTTCCACGATCCTTTTATGGGAAAAAGTGAAGCAGGTAAAATACAGAAAATTTTGAAGGTTGGCATGTTGCAAGACGCAGGAGGATACTTCTTCAACCTTCGTGTTTCTGACAGGACTCTCAATGTTGATGAGAGCTTGTTTATGCCTATAxxxxxxxxxxxxTCTCCGTTGTGCCGTTGGCGTTTCGATTTTATCTTGCCGTATCTCATGGGCTGGCATGCGTATATGAACTCCGAAAAACCCTATGAATCTGGCCACTTCACGTCTGCTAGTCCTAGTATGGGAAAAAAGCCTGATTTAGAGTGGGGTAGGTAGTTTAATTTTGCAGGAAGATTACGCAGAGCTCAGGTTAAAGTACTTTAACTGTATATTAACTGTTTGTAATGCTTACCCAGATGTGCCATTTTAATGGCTAAGACGATCGTAATTCACTGGAGGGAAAAGGTGTTGATTTTGGAAGGGATTGATTTGCAAGTGCTAGATATATTGGAGCAGAGGGATTCTCTTCCAATCTTCACTAGTTCGTCCATCCAATGGATTAAATGTTTTAGATCAATGGTGTTAGGGGCACACTGAACATTGATGATCCGCCTGTAGTTTTATTTAGAACTATTGTAAATTGTCTGATTCATGGAGGTTTCGTTGATGACAGTTTATATCTCGTTACCATGTACGATGAAGATTTAAGGGTTTTCCTGAAAATTTTGTAG

Fln Alignment:

Contig21516___AVTQPGFLRGQPSKIYVKYTLYKGKAALGIQPKAPEYTTLDSGGIRLSKEGCVFLEFAPAVGTRQYDWSKKQIFALSVLELGTLLSFDPKEPCEFFHDPFMGKSEAGKIQKILKVGMLQDAGGYFFNLRVSDRTLNVDESLFMPITxxNSPLCRWRFDFILPYLMGWHAYMNSEKPYESGHFTSASPSMGKKPDLEWGR
A9NLD1_______________ALTQPEFLRRQTNKIYVKHTVYKGEGALTMKPKLPDYITLNMGGVTVAKEGCMFLEFAPAVGPRQYDWSKKKIIALSVVEVGTLLSLGPDESCEFTHDPFMGKSEAGKIMKVLKVGNLQDTGGYFFNLSVTDRIADVDESFSIPITxxEFSVMQSIFNFILPYLMGWHAYMDSTKLNESGHFKSGGPSIAKRPDLEWGR

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
392			10	90	0.90298
394	90		10		0.90298
397	10			90	0.90298
398	90		10		0.90298
401	10			90	0.90298
403		90	10		0.90298
404		90	10		0.90298
405		90		10	0.90298
409			10	90	0.90298
411			10	90	0.90298
418		11	66	22	0.999999
419	11		88		0.923564
420	22	66		11	0.999999
421			22	77	0.999999
423			25	75	1.0
424	25			75	1.0
425		25		75	1.0
426	25			75	1.0
427		75	25		1.0
431	25			75	1.0
432	75		25		1.0
433		75	25		1.0
437		25	75		1.0
441			25	75	1.0
442	83			16	0.994512
444	16	83			0.994512
445	83		16		0.994512
450		16		83	0.994512
451		16	83		0.994512
457			83	16	0.994512
459	16	83			0.994512
460	83	16			0.994512
461	16		83		0.994512
463		16	16	66	0.988813
464			16	83	0.994512
465		80	20		0.995493
467		20		80	0.995493
470		20	80		0.995493
471		20	80		0.995493
472	20			80	0.995493
473	20			80	0.995493
474			20	80	0.995493
475			25	75	0.996473
478	25		75		0.996473
512	66		33		0.997452
513	33	66			0.997452
515			66	33	0.997452
516		66	33		0.997452
521	66	33			0.997452
522		33		66	0.997452
525	66		33		0.997452
526	66		33		0.997452
528		33	33	33	0.997753
530	33		66		0.997452
531	33	33		33	0.997753
532	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain