SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene21399

Length: 1254 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene21399 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam05193, Peptidase_M16_C, Peptidase M16 inactive domain	-	-	3.0e-06	21%
FL-Next	sp=Zinc-metallopeptidase, peroxisomal; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	30%
Blast2go	insulin-degrading enzyme-like	-	-	0.0	72%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 32	IPR001362	-	-
Sma3	Peptidase M16, zinc-binding site	IPR001431	-	-
Sma3	Peptidase M16, C-terminal	IPR007863	-	-
Sma3	Peptidase M16, core	IPR011237	-	-
Sma3	Peptidase M16, N-terminal	IPR011765	-	-
Sma3	Glycosyl hydrolases family 32, N-terminal	IPR013148	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: sp_plants

Fln subject: O22941

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 126 aas, your sequence is shorter than subject: 402 - 970

Fln protein: FNPHNMRVDILTKSFDCQSSGVQLEPWFGVPYRIENIPSTLIEDWENPPIVDPSIHLPDKNDFIPCDFSIKSLSDINIHGTMEIPICIIDDPLIKIWYKLDRTFNVPRANTYFLITLKEAYKSTRTCVLTELYVNLLRDALNETLYQANVARLDTTFSVLGDKLELKISGFNEKLPALASKILKFLTTFTPAADRFEVMKEDMERGYVNTNMKPLKHSAYLRLQILRKDFWHVDDKLSCLLSLTLADLITFIPELFSQIYIEGLCHGNLLKDEAISLANMFKDLFSATVLPSELRHAEQILRLPSGIGLLQNANVKNKSEENSSIEIYFQVDQDIGQSSNRARAIADLFEDIVHEPCFNQLRTKEQLGYVVDCGSRMTHRVLGFCSVFNLPSIAHYIYRKD*

Protein Length: 403

Fln nts: CTTCAATCCACATAATATGAGAGTTGATATATTGACAAAATCTTTTGATTGCCAATCTTCAGGTGTCCAGCTTGAACCATGGTTTGGTGTACCCTACCGTATTGAAAATATTCCGTCTACTCTTATAGAGGATTGGGAAAATCCCCCTATTGTAGACCCATCTATACATTTACCAGATAAGAATGATTTCATCCCATGTGATTTTTCAATTAAAAGCTTATCTGACATCAACATCCATGGCACGATGGAAATTCCAATATGTATTATTGATGATCCATTGATCAAGATTTGGTATAAACTAGATCGAACATTTAATGTTCCACGTGCAAATACATACTTCTTGATCACTTTAAAAGAGGCTTATAAAAGCACAAGGACTTGTGTACTCACTGAGTTGTATGTGAACCTTCTCCGGGATGCGCTCAACGAGACACTATATCAGGCAAATGTTGCTAGATTGGACACAACATTTTCTGTTTTAGGAGACAAACTTGAGTTAAAGATTTCTGGGTTCAATGAAAAGCTACCAGCTTTAGCATCAAAAATTCTAAAATTCTTGACAACCTTTACACCTGCTGCGGATCGTTTTGAGGTGATGAAGGAGGACATGGAAAGAGGCTATGTGAATACCAACATGAAACCTTTAAAGCATTCAGCATATTTAAGATTACAGATTTTGCGAAAGGATTTTTGGCATGTGGATGACAAACTTTCTTGTCTCCTTTCACTGACTCTAGCAGACTTGATAACTTTTATCCCAGAACTTTTTAGCCAGATTTATATTGAAGGCCTCTGTCATGGTAACTTATTAAAGGATGAAGCTATTTCTCTTGCAAATATGTTCAAAGATTTATTTTCGGCAACAGTTTTGCCATCTGAGTTGCGGCATGCAGAGCAAATCCTGAGGCTGCCATCTGGCATTGGCCTACTTCAGAATGCAAATGTGAAGAATAAATCAGAGGAAAATTCTTCTATTGAGATTTATTTTCAAGTGGATCAAGATATTGGTCAAAGTTCAAATAGAGCAAGGGCCATAGCAGATCTCTTTGAAGATATTGTTCACGAGCCATGTTTTAATCAGCTCAGGACAAAGGAACAGCTTGGATATGTTGTGGATTGTGGCAGTAGGATGACACACCGTGTACTGGGATTTTGTTCCGTGTTCAATCTTCCAAGTATAGCCCACTATATTTACAGGAAAGACTAGATGCATTTATCGATAAATTACAGCAAATTCTGGGTGAGATGGATGAT

Fln Alignment:

Contig21399___NPHNMRVDILTKSFDCQSSGVQLEPWFGVPYRIENIPSTLIEDW-ENPPIVDPSIHLPDKNDFIPCDFSIKSLSDINIHGTMEIPICIIDDPLIKIWYKLDRTFNVPRANTYFLITLKEAYKSTRTCVLTELYVNLLRDALNETLYQANVARLDTTFSVLGDKLELKISGFNEKLPALASKILKFLTTFTPAADRFEVMKEDMERGYVNTNMKPLKHSA-YLRLQILRKDFWHVXXXXXXXXXXXXXXLITFIPELFSQIYIEGLCHGNLLKDEAISLANMFKDL-------FSATVLPSELRHAEQILRLPSGIGLLQNANVKNKSEENSSIEIYFQVDQDIGQSSNRARAIADLFEDIVHEPCFNQLRTKEQLGYVVDCGSRMTHRVLG
O22941_______________SPSNFRIFWESQKFEGQTD--KAEPWYNTAYSLEKITSSTIQEWVQSAP--DVHLHLPAPNVFIPTDLSLKDADDKET-----VPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNCPLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLGYNHKLRILLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQTWPWTEELDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDPKPICRPLFPSQ-HLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQ----LFGLVAKQATFHQLRTVEQLGYITALAQRNDSGIYG

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
538	85			14	0.993533
540			85	14	0.993533
549	85	14			0.993533
558	66		33		1.0
678	83	16			0.994512
682		14	85		0.993533
685			85	14	0.993533
687	50	50			1.0
1007	12		87		0.992554
1008	85	14			0.993533
1041		25		75	0.996473
1109		33		66	0.997452
1128	33		66		0.997452
1146		66		33	0.997452
1158	66	33			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain