SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene21274

Length: 2436 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene21274
ATGGGGCAGTAGCGGACGAGAAGCGTGTCGAGACATTAATATGTGATATTAAGAGTATGCTCAGAGGTATGGGTGATGGGGAAATAAGTCCCTCTGCGTATGACACTGCTTGGGTAGCAAAGATTCCAGCACTTGATGGCTCTGACCACCCTCACTTTCCTCAAACACTCCAATGGATTCTTCAGAATCAGCTGCAAGATGGCTCTTGGGGCGAAGACAACCATTTCCTAAGATATGACAGAGTACTCGCTACTCTTGCGTGTATTATTACCCTCACCGTATGGCGCACTGGCCAGATACAAGTGCAGAAAGGTCTTGAATTCTTCAAGAAACACGCTGAAATGATGGAGGATGAAGCGGACAATCACCGGCCGAGTGGATTCGAAATTGTCTTTCCTGCTATGCTAAACCAAGCAAAACAATTATGCTTGGATCTGCCTTATGAGTTGCCATTCATTAAACAAATCATTAAGAAGGCGGAAGCTAAGCTTAAAAGGATTCCCACTGACACTCTCTATACGGTTCCAACAATATACTTGTATTATTTGGAAGGCCTGCAAGAAATAGTGGAGTGGCCCAGAATCATCAAACTTCAATCAAAGGATGGATCGTTCCTCGGCTCGCCAGCATCTACAGCCGCTGTATTCATGCGTACAGGGAACACCAAGTGCTTGGAATTCTTGAACTTGGTCTTAACGAAATTCGGAAACCACGTGCCGTGTCACTATCCGCTTAATCTATTTGAACGGCTGTGGGCCATTGATACTGTTGAACGGTTAGGGATCGATCGCCATTTCAAAAAGGAAATCAAGCACGCATTGGATTATGTTTACAGCCATTGGAACGAAAGAGGCATTGGATGGGCGAGAGAGAATCATGTTGCTGATATTGATGTTACAGCCATGGGACTTCGAATCTTGAGACTGCATGGATACAATGTATCCGCAGATGTTTTAAAAACTTTTAGAGATGAGAATGGGGAGTTCTTTCGCTTTTCGGGTCAAACGGAGAGAGGAGTAACAGACATGTTAAGCCTCAATCGTTGCTCACACGTTGCGATACCAGGAGAAACCGTCATGGAAGAAGCACAACTCTGTACCGAAAGATATCTGTGGAGCGCTCTGGAAAATGTGGATGCGTTTGACAAATGGGCTCTTAAAAAGAACATTCGCGGAGAGGTGGAGTACGCCCTCAGACATCCATGGCATAGGAGTCTGCCAAGGCTGGAGGCTAGAAGCTACATTCAACATTATGGGTCAAACGATGTCTGGCTTGGGAAAACTATGTATATGATGCCATATATTAACAACCGAAAATACTTGGAACTGGCCAAACTGGACTTCAATTATGTGCAGTCCATACACCAAAAAGAGATTCACGAACTTCGAAGGTGGTGGAAGTCATCCGGTTTCGCGGAGCTCAATTTCACACGCGACCGTGTGACTGAAATATTTTTCTCAATCGCGTCTTCCCTGTTTGAACCAGAGCTCGCTTCGTGTAGAGCCATTTATACGAAATCTACCATCTTGATTGTCATTTTAGACGACCTATATGACGCACATGCATCTTTAGAAGACATCAAGCTGTTCACAGAAGCAGTTAAAAGATGGGATCTGTTTCTGTTAGACCGAATGCCAGAACACATCAAAACATGCTACCTGGGTTTGTACAATCTTGTCAATGAAATAGCAGAAGAAGGGCGCAAGAGACAGGGTCGTGACGTACTAGGCTACATTAGAAATTTATGGGAGATCCTCGTTGAAGCATATACGAAAGAAGCAGAATGGTCGGAAGCTGCATATGTGCCCTCCTTCAATGAATACATAGAGAACGCTACTGTATCAATATCCCTGGCAACTATCGTTCTGATCAGTGTTCTTTTCACTGGAGAGATTCTTACAGATCACATACTCTCCCAAATTGGTCACCGATCCAAATTTGTATATCTCATGTGCTTGACAGGGCGTTTGGTAAACGATACCAAAAGTTACCAGGCAGAGAGAGATCGAGGAGAGGTAGCTTCTTCCATACAGTGTTACATGAAGGACCATCCCGAATTGTCAGAGGAAGAAGCTCTCAAATATGTTTATATTCTATTGGGAAACGCCCTCGCAGATTTGAAGGGTGAATTTCTGACTACAAAAGAGGTGCCGGACAATATCAGAAGACTGATTTTTGACAATGCAAGATCATCGCAACTGTTTTGCATGGAAGAGGACGGTTTCACACATTCACATGAAACGGAAATTAAAGAGCATGTCAAAAGGATACTGTTCGAACCAGTTGCGTAGAATACATTACTCAAATAAAGGCCCGGCACTTTCATTTTGTACTGTTACCACACATACTTGCTCAGCGGGTATTATGACCATTGTGTTTTTCGCATTTTCTAATGCCAGCAATGAAGTTCATGGTTGCCCACGTGTTTCTGTGTTT

Ace file of the UniGene sp_v2.0_unigene21274 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Abietadiene synthase, chloroplastic n=1 Tax=Abies grandis RepID=TPSDV_ABIGR	-	-	74%
FL-Next	tr=Levopimaradiene/abietadiene synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	76%
Blast2go	a chain structure of a three-domain sesquiterpene synthase: a prospective target for advanced biofuels production	-	-	79%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: F2XFB2

Fln msg: your sequence is shorter than subject: 763 - 859

Fln protein: GAVADEKRVETLICDIKSMLRGMGDGEISPSAYDTAWVAKIPALDGSDHPHFPQTLQWILQNQLQDGSWGEDNHFLRYDRVLATLACIITLTVWRTGQIQVQKGLEFFKKHAEMMEDEADNHRPSGFEIVFPAMLNQAKQLCLDLPYELPFIKQIIKKAEAKLKRIPTDTLYTVPTIYLYYLEGLQEIVEWPRIIKLQSKDGSFLGSPASTAAVFMRTGNTKCLEFLNLVLTKFGNHVPCHYPLNLFERLWAIDTVERLGIDRHFKKEIKHALDYVYSHWNERGIGWARENHVADIDVTAMGLRILRLHGYNVSADVLKTFRDENGEFFRFSGQTERGVTDMLSLNRCSHVAIPGETVMEEAQLCTERYLWSALENVDAFDKWALKKNIRGEVEYALRHPWHRSLPRLEARSYIQHYGSNDVWLGKTMYMMPYINNRKYLELAKLDFNYVQSIHQKEIHELRRWWKSSGFAELNFTRDRVTEIFFSIASSLFEPELASCRAIYTKSTILIVILDDLYDAHASLEDIKLFTEAVKRWDLFLLDRMPEHIKTCYLGLYNLVNEIAEEGRKRQGRDVLGYIRNLWEILVEAYTKEAEWSEAAYVPSFNEYIENATVSISLATIVLISVLFTGEILTDHILSQIGHRSKFVYLMCLTGRLVNDTKSYQAERDRGEVASSIQCYMKDHPELSEEEALKYVYILLGNALADLKGEFLTTKEVPDNIRRLIFDNARSSQLFCMEEDGFTHSHETEIKEHVKRILFEPVA*

Protein Length: 764

Fln nts: ATGGGGCAGTAGCGGACGAGAAGCGTGTCGAGACATTAATATGTGATATTAAGAGTATGCTCAGAGGTATGGGTGATGGGGAAATAAGTCCCTCTGCGTATGACACTGCTTGGGTAGCAAAGATTCCAGCACTTGATGGCTCTGACCACCCTCACTTTCCTCAAACACTCCAATGGATTCTTCAGAATCAGCTGCAAGATGGCTCTTGGGGCGAAGACAACCATTTCCTAAGATATGACAGAGTACTCGCTACTCTTGCGTGTATTATTACCCTCACCGTATGGCGCACTGGCCAGATACAAGTGCAGAAAGGTCTTGAATTCTTCAAGAAACACGCTGAAATGATGGAGGATGAAGCGGACAATCACCGGCCGAGTGGATTCGAAATTGTCTTTCCTGCTATGCTAAACCAAGCAAAACAATTATGCTTGGATCTGCCTTATGAGTTGCCATTCATTAAACAAATCATTAAGAAGGCGGAAGCTAAGCTTAAAAGGATTCCCACTGACACTCTCTATACGGTTCCAACAATATACTTGTATTATTTGGAAGGCCTGCAAGAAATAGTGGAGTGGCCCAGAATCATCAAACTTCAATCAAAGGATGGATCGTTCCTCGGCTCGCCAGCATCTACAGCCGCTGTATTCATGCGTACAGGGAACACCAAGTGCTTGGAATTCTTGAACTTGGTCTTAACGAAATTCGGAAACCACGTGCCGTGTCACTATCCGCTTAATCTATTTGAACGGCTGTGGGCCATTGATACTGTTGAACGGTTAGGGATCGATCGCCATTTCAAAAAGGAAATCAAGCACGCATTGGATTATGTTTACAGCCATTGGAACGAAAGAGGCATTGGATGGGCGAGAGAGAATCATGTTGCTGATATTGATGTTACAGCCATGGGACTTCGAATCTTGAGACTGCATGGATACAATGTATCCGCAGATGTTTTAAAAACTTTTAGAGATGAGAATGGGGAGTTCTTTCGCTTTTCGGGTCAAACGGAGAGAGGAGTAACAGACATGTTAAGCCTCAATCGTTGCTCACACGTTGCGATACCAGGAGAAACCGTCATGGAAGAAGCACAACTCTGTACCGAAAGATATCTGTGGAGCGCTCTGGAAAATGTGGATGCGTTTGACAAATGGGCTCTTAAAAAGAACATTCGCGGAGAGGTGGAGTACGCCCTCAGACATCCATGGCATAGGAGTCTGCCAAGGCTGGAGGCTAGAAGCTACATTCAACATTATGGGTCAAACGATGTCTGGCTTGGGAAAACTATGTATATGATGCCATATATTAACAACCGAAAATACTTGGAACTGGCCAAACTGGACTTCAATTATGTGCAGTCCATACACCAAAAAGAGATTCACGAACTTCGAAGGTGGTGGAAGTCATCCGGTTTCGCGGAGCTCAATTTCACACGCGACCGTGTGACTGAAATATTTTTCTCAATCGCGTCTTCCCTGTTTGAACCAGAGCTCGCTTCGTGTAGAGCCATTTATACGAAATCTACCATCTTGATTGTCATTTTAGACGACCTATATGACGCACATGCATCTTTAGAAGACATCAAGCTGTTCACAGAAGCAGTTAAAAGATGGGATCTGTTTCTGTTAGACCGAATGCCAGAACACATCAAAACATGCTACCTGGGTTTGTACAATCTTGTCAATGAAATAGCAGAAGAAGGGCGCAAGAGACAGGGTCGTGACGTACTAGGCTACATTAGAAATTTATGGGAGATCCTCGTTGAAGCATATACGAAAGAAGCAGAATGGTCGGAAGCTGCATATGTGCCCTCCTTCAATGAATACATAGAGAACGCTACTGTATCAATATCCCTGGCAACTATCGTTCTGATCAGTGTTCTTTTCACTGGAGAGATTCTTACAGATCACATACTCTCCCAAATTGGTCACCGATCCAAATTTGTATATCTCATGTGCTTGACAGGGCGTTTGGTAAACGATACCAAAAGTTACCAGGCAGAGAGAGATCGAGGAGAGGTAGCTTCTTCCATACAGTGTTACATGAAGGACCATCCCGAATTGTCAGAGGAAGAAGCTCTCAAATATGTTTATATTCTATTGGGAAACGCCCTCGCAGATTTGAAGGGTGAATTTCTGACTACAAAAGAGGTGCCGGACAATATCAGAAGACTGATTTTTGACAATGCAAGATCATCGCAACTGTTTTGCATGGAAGAGGACGGTTTCACACATTCACATGAAACGGAAATTAAAGAGCATGTCAAAAGGATACTGTTCGAACCAGTTGCGTAG___AATACATTACTCAAATAAAGGCCCGGCACTTTCATTTTGTACTGTTACCACACATACTTGCTCAGCGGGTATTATGACCATTGTGTTTTTCGCATTTTCTAATGCCAGCAATGAAGTTCATGGTTGCCCACGTGTTTCTGTGTTT

Fln Alignment:

Contig21274___AVADEKRVETLICDIKSMLRGMGDGEISPSAYDTAWVAKIPALDGSDHPHFPQTLQWILQNQLQDGSWGEDNHFLRYDRVLATLACIITLTVWRTGQIQVQKGLEFFKKHAEMMEDEADNHRPSGFEIVFPAMLNQAKQLCLDLPYELPFIKQIIKKAEAKLKRIPTDTLYTVPTIYLYYLEGLQEIVEWPRIIKLQSKDGSFLGSPASTAAVFMRTGNTKCLEFLNLVLTKFGNHVPCHYPLNLFERLWAIDTVERLGIDRHFKKEIKHALDYVYSHWNERGIGWARENHVADIDVTAMGLRILRLHGYNVSADVLKTFRDENGEFFRFSGQTERGVTDMLSLNRCSHVAIPGETVMEEAQLCTERYLWSALENVDAFDKWALKKNIRGEVEYALRHPWHRSLPRLEARSYIQHYGSNDVWLGKTMYMMPYINNRKYLELAKLDFNYVQSIHQKEIHELRRWWKSSGFAELNFTRDRVTEIFFSIASSLFEPELASCRAIYTKSTILIVILDDLYDAHASLEDIKLFTEAVKRWDLFLLDRMPEHIKTCYLGLYNLVNEIAEEGRKRQGRDVLGYIRNLWEILVEAYTKEAEWSEAAYVPSFNEYIENATVSISLATIVLISVLFTGEILTDHILSQIGHRSKFVYLMCLTGRLVNDTKSYQAERDRGEVASSIQCYMKDHPELSEEEALKYVYILLGNALADLKGEFLTTKEVPDNIRRLIFDNARSSQLFCMEEDGFTHSHETEIKEHVKRILFEPVA
F2XFB2_______________AASDEKRIETLISEIKNMFRSMGYGETNPSAYDTAWVARIPAVDGSEQPEFPETLEWILQNQLKDGSWGEGFYFLAYDRILATLACIITLTLWRTGEIQVQKGIEFFKTQAVKIEDEADSHRPSGFEIVFPAMLKEAKVLGLDLPYELPFIKKIIEKREAKLERLPTNILYALPTTLLYSLEGLQEIVDWQKIIKLQSKDGSFLTSPASTAAVFMRTGNKKCLEFLNFVLKKFGNHVPCHYPLDLFERLWAVDTVERLGIDRHFKEEIKDALDYVYSHWDERGIGWARENLVPDIDDTAMGLRILRLHGYNVSSDVLKTFRDENGEFFCFLGQTQRGVTDMLNVNRCSHVAFPGETIMEEAKTCTERYLRNALEDVGAFDKWALKKNIRGEVEYALKYPWHRSMPRLEARSYIEHYGPNDVWLGKTMYMMPYISNEKYLELAKLDFNHVQSLHQKELRDLRRWWTSSGFTELKFTRERVTEIYFSPASFMFEPEFATCRAVYTKTSNFTVILDDLYDAHGTLDDLKLFSDSVKKWDLSLVDRMPEDMKICFMGFYNTFNEIAEEGRKRQGRDVLGYIRNVWEIQLEAYTKEAEWSAARYVPSFDEYIENASVSIALGTVVLISALFTGEILTDDVLSKIGRGSRFLQLMGLTGRLVNDTKTYEAERGQGEVASAVQCYMKDHPEISEEEALKHVYTVMENALDELNREFVNNREVPDSCRRLVFETARIMQLFYMDGDGLTLSHETEIKEHVKNCLFHPVA

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
30		33		66	0.997452
315		33		66	1.0
355	33		66		1.0
1959		20	80		0.995493
2024		25		75	0.996473
2063		83		16	0.994512
2066		83	16		0.994512
2075			80	20	0.995493
2100	14		85		0.993533
2129		85		14	0.993533
2149		14		85	0.993533
2174	85		14		0.993533
2189	33		66		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain