SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene20704

Length: 1133 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uagpf v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene20704

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Phospholipase D n=1 Tax=Citrus sinensis RepID=B2L042_CITSI	-	-	79%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	phospholipase d alpha	-	-	90%

Source	InterPros	Term	Type	Identity
Sma3	C2 calcium-dependent membrane targeting	IPR000008	-	-
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Phospholipase D/Transphosphatidylase	IPR001736	-	-
Sma3	YLP motif	IPR004019	-	-
Sma3	Phospholipase D, plant	IPR011402	-	-
Sma3	Phospholipase D	IPR015679	-	-
Sma3	C2 membrane targeting protein	IPR018029	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NV59

Fln msg: Separated hits, possible frame ERROR between 621 and 626, Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 328 - 482

Fln protein: RRDLGCHSTIPLELRSFDVEHYVPLMLCIYLKFWNFNI*ERFLTWNPWSSLTIF*VWMYLHTL*DEFRCH**FLKSCCVFICPSIHLHISFDNS*MYLHVSFDNPQNSEMWSSIRTSQNFGGEPIVIFFCEYLLLYNFCSATY*LFNVLVA*V*FDMLEF*SVGFLKMICFF*YPACFFYAVDDEYIIIGSANINQRSMDGARDSExxxSMGAYQPYHLAVKEPARGQIHGFRMSLWYEHTGLLDNLFLQPESLECIRKMNKIAERYWDLYSSESLERDLPGHLLMYPVGVTNAGEVTELPGTEFFPDTKARVLGTKSDYLPPILTT*

Protein Length: 329

Fln nts: CGTCGTGACCTTGGCTGTCACTCAACCATACCACTTGAGCTACGTTCCTTTGACGTTGAACATTATGTGCCATTAATGTTATGTATATATTTAAAATTTTGGAATTTTAATATTTAAGAAAGGTTCTTAACGTGGAATCCCTGGTCCTCATTGACAATCTTTTAAGTTTGGATGTACCTCCATACACTTTAGGATGAATTTCGGTGTCATTGATAATTTTTAAAATCTTGTTGTGTCTTCATATGCCCCAGTATTCATCTCCATATCTCGTTTGACAATTCTTAAATGTATCTCCATGTCTCGTTTGACAATCCTCAAAATTCAGAAATGTGGAGCAGCATCCGCACATCCCAAAATTTTGGTGGAGAACCCATTGTAATCTTTTTCTGTGAATACCTCTTGCTCTATAATTTCTGTTCTGCAACTTATTAACTTTTCAATGTACTGGTGGCATGAGTCTGATTTGATATGCTTGAATTTTAGTCCGTGGGATTTCTCAAGATGATATGCTTTTTCTGATACCCGGCTTGTTTCTTTTATGCAGTTGACGATGAGTACATTATTATTGGATCAGCAAATATCAACCAACGTTCCATGGATGGTGCAAGAGACTCTGAGATxxxxxxTTCAATGGGGGCTTACCAACCATATCATCTGGCAGTTAAaGAGCCAGCCAGAGGGCAAATCCATGGTTTCAGGATGTCTCTCTGGTACGAACACACTGGATTGCTAGATAACTTGTTCCTTCAGCCCGAAAGCCTAGAATGTATCAGGAAAATGAACAAAATAGCAGAACGATACTGGGATTTATATAGCAGTGAAAGTTTGGAACGTGATCTGCCGGGCCATTTGTTGATGTATCCTGTGGGTGTCACAAATGCTGGTGAAGTGACAGAGTTACCTGGTACAGAATTCTTTCCAGATACAAAGGCTCGTGTATTGGGCACCAAATCAGACTACCTGCCACCCATTCTGACCACATAGAGAGTTGGAGCGGTAGTACTCCAATAGCTGTTTGGTCTTTCGTAACACATACTATATTTAGAGCTCTTGCCCTTCTTTTTTTCCCGTGTTGGTATTTGCACTAAAATACAGAGTCGTCTTTAGAGCTCACACAAAGCTTGTGGAAATATCT

Fln Alignment:

Contig20704___VDDEYIIIGSANINQRSMDGARDSEIxxSMGAYQPYHLAVKEPARGQIHGFRMSLWYEHTGLLDNLFLQPESLECIRKMNKIAERYWDLYSSESLERDLPGHLLMYPVGVTNAGEVTELPGTEFFPDTKARVLGTKSDYLPPILTT
A9NV59_______________VDDEYILIGSANINQRSMDGARDSEIxxAMGAYQPFHLAVNEPARGQIHGFRMSLWYEHTGMLDNLFLQPENVQCIRKVNKIAERYWDLYSSESLERDLPGHLLMYPVGITNEGEVTELPGSEFFPDTKARIFGTKSDLLPPILTT

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
725		75	25		0.996473
726			25	75	0.996473
834		11	88		0.923564
835	88		11		0.923564
836	88			11	0.923564
842		11		88	0.923564
846		87	12		0.992554
848			87	12	0.992554
850			87	12	0.992554
853	87	12			0.992554
854	12			87	0.992554
908	14			85	0.993533
909		14	85		0.993533
910	14		85		0.993533
912	85			14	0.993533
983		83		16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain