SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene20224

Length: 1416 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene20224 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectin lyase-like protein [Arabidopsis thaliana] gb\|AAB71972.1\| putative polygalacturonase [Arabidopsis thaliana] gb\|AEE33700.1\| Pectin lyase-like protein [Arabidopsis thaliana]	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	43%
Blast2go	polygalacturonase at1g48100-like	-	-	75%

Source	InterPros	Term	Type	Identity
Sma3	Ribosome-binding factor A	IPR000238	-	-
Sma3	Regulator of chromosome condensation, RCC1	IPR000408	-	-
Sma3	Glycoside hydrolase, family 28	IPR000743	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Parallel beta-helix repeat	IPR006626	-	-
Sma3	Tetratricopeptide-like helical	IPR011990	-	-
Sma3	Pectin lyase fold	IPR012334	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQX9

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 18 aas, your sequence is shorter than subject: 395 - 493

Fln protein: KIFDVLSYGAKGDGVTDDTKAFQIAWQTACNVESAIVEVPAEFRFLVGPISFSGPCQPKITFQIDGTIIAPVNPRVWGPAGMLQWIQFSKLNSITIQGCGVIDGRGSVWWGSSSCEINSQKGKRMPNIKPTALRFYGSIGATVTGITIQNSPQCHLKFDSCTSVEVSSVTISSPSDSPNTDGIHLQNSQDVEIHHSSMACGDDCVSIQTGCSNVRVHNINCGPGHGISIGGLGKDQTKACVSNITVYNSSIGDTLNGVRIKTWQGGLGSVKGVSFSNIQVSNVKIPIVIDQFYCDKSVCTNQSAAVAISGVTYENIRGTYAYKAVHLACSDDVPCTQVVLTDIELHPVVQNLRQPFCWKTYGEAQNPSIPPIYCLHTGQPYKNPGSIQSNQDGC*

Protein Length: 396

Fln nts: AAAATTTTTGATGTGCTTTCCTATGGCGCCAAGGGAGATGGAGTGACGGATGATACCAAGGCTTTCCAGATTGCTTGGCAGACAGCCTGTAATGTGGAGTCAGCTATAGTAGAAGTACCTGCTGAGTTTCGGTTTCTTGTTGGACCCATTTCATTTTCTGGACCTTGTCAGCCTAAAATCACCTTTCAGATTGATGGGACAATCATAGCACCAGTGAATCCTCGTGTATGGGGGCCTGCAGGGATGCTACAGTGGATACAGTTTAGTAAATTGAATAGCATTACCATTCAGGGATGTGGGGTCATTGATGGAAGAGGATCAGTTTGGTGGGGATCTTCTAGCTGTGAGATAAACAGCCAAAAGGGAAAACGAATGCCAAACATTAAGCCCACTGCATTGAGATTCTACGGAAGCATAGGTGCGACTGTAACTGGCATAACCATTCAAAACAGCCCGCAGTGTCACCTTAAATTCGACAGTTGCACTTCCGTCGAAGTTTCCAGCGTCACAATCTCTTCTCCGTCGGACAGCCCAAATACAGATGGAATACATCTTCAGAATTCTCAGGACGTTGAAATACATCATTCCTCAATGGCATGCGGTGATGACTGCGTTTCTATACAAACAGGATGTTCAAACGTACGCGTGCACAACATTAACTGCGGACCTGGTCATGGTATCAGCATTGGAGGACTCGGAAAGGACCAAACAAAAGCATGTGTTTCCAATATCACTGTCTACAACAGTTCTATTGGAGATACCTTAAACGGAGTTAGAATCAAGACATGGCAGGGAGGACTAGGGTCGGTAAAAGGAGTTTCCTTCTCCAACATTCAAGTCTCCAACGTGAAAATTCCAATTGTGATCGATCAATTTTACTGTGACAAAAGTGTTTGCACCAATCAGAGCGCAGCTGTTGCAATATCAGGCGTCACATACGAGAATATTCGAGGAACATATGCCTACAAAGCCGTCCATCTCGCCTGCAGTGATGATGTACCTTGCACACAAGTGGTTTTAACAGATATTGAACTTCATCCCGTGGTGCAGAACCTACGGCAGCCATTCTGCTGGAAGACTTACGGCGAAGCTCAGAACCCATCAATACCACCAATTTATTGCTTGCATACCGGACAACCTTACAAGAATCCTGGCAGCATACAATCAAACCAGGATGGTTGCTAG___TTTTTTTTTACCTGGCATAATCTTAGGTGGGTTTATCAGGAGGGGCCACATGTTTAGTCCACTCAACCATGATTTTGATAAACGGAAAGAGAATTGAACTCACGCTTCATGACATAAGACGAATACATCACAGTTAAGTTGGTTGGTAGGGTTAAAATGGTGGATAGTTACCATATTTAGGTTTCTGCGGCCATTGGCACATGTACATGATAATTTGGGTATTGTAATAAA

Fln Alignment:

Contig20224___YGAKGDGVTDDTKAFQIAWQTACNVESAIVEVPAEFRFLVGPISFSGPCQPKITFQIDGTIIAPVNPRVWGPAGMLQWIQFSKLNSITIQGCGVIDGRGSVWWGSSSCEINSQKGKRMPNIKPTALRFYGSIGATVTGITIQNSPQCHLKFXXXXXXXXXXXXXXXXXXXPNTDGIHLQNSQDVEIHHSSMACGDDCVSIQTGCSNVRVHNINCXXXXXXXXXXXGKDQTKACVSNITVYNSSIGDTLNGVRIKTWQGGLGSVKGVSFSNIQVSNVKIPIVIDQFYCD-KSVCTNQSAAVAISGVTYENIRGTYAYKAV-HLACSDDVPCTQVVLTDIELHPVVQNLRQPFCWKTYGEAQNPSIPPIYCLHTGQPYKNPG
B8LQX9_______________FGAKGDGENDDTKAFNNAWSKACSSAPAVFLVPHGKKYLVKPIKFSGPCKSALTMQVSGTIIAPADPRVWDGLNRRQWLRFKEVDDLTVEGGGIFYGSGEQWW-AESCKINKTNPCRP---APTAITFELCNNLRVMNLIVQNSQQMHIKFRKCVGVKADNLKVLAPGHSPNTDGIHVSASKDVVIKNTITGTGDDCISIVSDSFNIYIENITCGPGHGISIGSLGEGSAKARVADVMVHGAFLHNTTNGLRIKTWQGSSGSARRILFQNVHMENVKHPIIIDQYYCDSKKPCSNKTSAVKVSEVAYLNIKGTSASKEVMRFACSQTVPCENILLADIDLTATSGCTPTTYCENAIG-CTTGSVSPHSCL---QNYNQKG

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
22	33			66	0.997452
46	33		66		0.997452
66	66		33		0.997452
72	33	66			0.997452
842		18		81	0.999994
956	12		87		0.992554
958		85	14		0.993533
961	14			85	0.993533
990			16	83	0.994512
995		16		83	0.994512
998		83	16		0.994512
999	66		16	16	0.988813
1002			16	83	0.994512
1004		16		83	0.994512
1231		16		83	0.994512
1288		80		20	0.995493
1291	80		20		0.995493
1292	80			20	0.995493
1293		80	20		0.995493
1294	20			80	0.995493
1308			20	80	0.995493
1309	20			80	0.995493
1311			16	83	0.994512
1340		40		60	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain